MytuD.17532.a

Protease-related protein

CENTER ID: MytuD.17532.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.17532.a.A1.GE30994 Full length( MytuD.17532.a ) 1 341
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.1162
RefSeq: NP_215559.1
TubercuList: Rv1043c
MTB Network Portal: Rv1043c
UniProt: P96358

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MCAHQFFGLV HNPVVAAAIG KPEPPPVDSD IGLPTTVPFE PWSVADFSRY LSTLGLPAAG DAVTLHRILS SMERAGLLLP LGWDPRLPVM GQKYISQGAI SKGQRGGNLW LSEVFGAELI IPSYNAVTVQ LAGHDDAGNP VDSWGTGLVV DHNHVITNKH VVTGLAGTSA GLSVYPSSNH AEAELVNFSG TAHPHPTLDV AVIKFEMPEG KYIPRLGGMA FRDPDWADEV YVFGYPRVPM TAEMAITVQR GEVVNPAATT IPGRQKIFLY SAIARPGNSG GPIVAQDGRV IGLVVEDSAE APSTGTGPNA APFYRGIPSS EVIRALDELD FGGIVEMDTL P
NT Sequence
ATGTGCGCCC ATCAATTCTT CGGGCTGGTC CACAACCCGG TGGTTGCGGC GGCGATCGGC AAACCCGAAC CACCACCGGT TGACTCGGAT ATCGGTCTGC CGACGACGGT GCCCTTCGAA CCGTGGTCGG TCGCAGATTT CTCCCGATAC CTGAGCACCC TCGGCCTGCC CGCCGCGGGC GACGCGGTGA CCCTCCACCG CATCCTCAGC TCGATGGAAC GCGCGGGACT TCTGCTGCCG CTCGGTTGGG ACCCGCGATT GCCAGTCATG GGGCAGAAGT ACATCTCGCA GGGGGCGATA TCGAAGGGAC AGCGTGGCGG CAACCTCTGG TTGTCCGAGG TGTTCGGTGC CGAGCTCATC ATCCCGAGCT ACAACGCGGT GACCGTTCAA CTCGCCGGTC ACGACGACGC TGGCAATCCG GTGGATAGTT GGGGAACCGG CCTCGTCGTC GACCACAATC ACGTCATCAC CAACAAACAT GTCGTGACCG GGCTCGCGGG CACGAGCGCA GGTCTGTCCG TCTATCCGTC AAGCAACCAC GCCGAAGCCG AGTTGGTGAA CTTCTCGGGC ACCGCTCACC CGCACCCCAC GCTCGACGTC GCAGTAATCA AATTCGAGAT GCCCGAAGGC AAGTACATCC CGCGGCTTGG CGGCATGGCG TTCCGTGATC CCGACTGGGC GGATGAGGTC TACGTGTTCG GTTACCCGCG GGTGCCGATG ACTGCGGAGA TGGCGATCAC GGTGCAGCGT GGCGAGGTGG TCAACCCGGC CGCGACGACT ATCCCCGGCC GGCAGAAGAT TTTCCTCTAC TCGGCAATCG CGCGGCCGGG CAACAGCGGC GGTCCGATCG TTGCGCAAGA CGGCCGTGTG ATCGGCCTCG TGGTCGAGGA CTCTGCGGAA GCACCGTCGA CGGGCACCGG ACCGAACGCG GCACCCTTCT ATCGCGGCAT CCCGTCGAGC GAAGTGATCC GAGCACTCGA CGAACTCGAC TTCGGCGGCA TCGTGGAGAT GGACACCCTC CCA
Details for MytuD.17532.a.A1.GE30994
HARVESTED ON: 12/14/2010
SEQUENCED ON: 12/20/2010
EXPECTED MW: 38kDa
OBSERVED MW: 38kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 87
PERCENT COVERAGE: 106
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMCAHQFFGL VHNPVVAAAI GKPEPPPVDS DIGLPTTVPF EPWSVADFSR YLSTLGLPAA GDAVTLHRIL SSMERAGLLL PLGWDPRLPV MGQKYISQGA ISKGQRGGNL WLSEVFGAEL IIPSYNAVTV QLAGHDDAGN PVDSWGTGLV VDHNHVITNK HVVTGLAGTS AGLSVYPSSN HAEAELVNFS GTAHPHPTLD VAVIKFEMPE GKYIPRLGGM AFRDPDWADE VYVFGYPRVP MTAEMAITVQ RGEVVNPAAT TIPGRQKIFL YSAIARPGNS GGPIVAQXXR VIGXVXXXLX KXXXGXXXER XXXXXASXXA XDXALXXXDX XXXXXGXXXX XXXXXXXXXX XXXXCXXXXX XXXXXXX
Validated NT Sequence
ttttgtttnn ctttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgtgcg cccatcaatt cttcgggctg gtccacaacc cggtggttgc ggcggcgatc ggcaaacccg aaccaccacc ggttgactcg gatatcggtc tgccgacgac ggtgcccttc gaaccgtggt cggtcgcaga tttctcccga tacctgagca ccctcggcct gcccgccgcg ggcgacgcgg tgaccctcca ccgcatcctc agctcgatgg aacgcgcggg acttctgctg ccgctcggtt gggacccgcg attgccagtc atggggcaga agtacatctc gcagggggcg atatcgaagg gacagcgtgg cggcaacctc tggttgtccg aggtgttcgg tgccgagctc atcatcccga gctacaacgc ggtgaccgtt caactcgccg gtcacgacga cgctggcaat ccggtggata gttggggaac cggcctcgtc gtcgaccaca atcacgtcat caccaacaaa catgtcgtga ccgggctcgc gggcacgagc gcaggtctgt ccgtctatcc gtcaagcaac cacgccgaag ccgagttggt gaacttctcg ggcaccgctc acccgcaccc cacgctcgac gtcgcagtaa tcaaattcga gatgcccgaa ggcaagtaca tcccgcggct tggcggcatg gcgttccgtg atcccgactg ggcggatgag gtctacgtgt tcggttaccc gcgggtgccg atgactgcgg agatggcgat cacggtgcag cgtggcgagg tggtcaaccc ggccgcgacg actatccccg gccggcagaa gattttcctc tactcggcaa tcgcgcggcc gggcaacagc ggcggtccga tcgttgcgca agangnccgt gtgatcggcn tcgtggncan nantctgcng aagcannncg angggcancn gnacgaacgc ngcancncnn tcncggcatc ccnncnagcg nntgatcnng cactcnacnn annngacntc nnnnnntnnn nnnntggnnn ccnnnccnnn nnnannnnnn nnnnatnnnn cannnnnnnn nnnnnnnnnn nntgctannn nnnnnnnnnn nnnnnaannn nnnnnnnnan na
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMCAHQFFGL VHNPVVAAAI GKPEPPPVDS DIGLPTTVPF EPWSVADFSR YLSTLGLPAA GDAVTLHRIL SSMERAGLLL PLGWDPRLPV MGQKYISQGA ISKGQRGGNL WLSEVFGAEL IIPSYNAVTV QLAGHDDAGN PVDSWGTGLV VDHNHVITNK HVVTGLAGTS AGLSVYPSSN HAEAELVNFS GTAHPHPTLD VAVIKFEMPE GKYIPRLGGM AFRDPDWADE VYVFGYPRVP MTAEMAITVQ RGEVVNPAAT TIPGRQKIFL YSAIARPGNS GGPIVAQDGR VIGLVVEDSA EAPSTGTGPN AAPFYRGIPS SEVIRALDEL DFGGIVEMDT LP
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtgcgcccat caattcttcg ggctggtcca caacccggtg gttgcggcgg cgatcggcaa acccgaacca ccaccggttg actcggatat cggtctgccg acgacggtgc ccttcgaacc gtggtcggtc gcagatttct cccgatacct gagcaccctc ggcctgcccg ccgcgggcga cgcggtgacc ctccaccgca tcctcagctc gatggaacgc gcgggacttc tgctgccgct cggttgggac ccgcgattgc cagtcatggg gcagaagtac atctcgcagg gggcgatatc gaagggacag cgtggcggca acctctggtt gtccgaggtg ttcggtgccg agctcatcat cccgagctac aacgcggtga ccgttcaact cgccggtcac gacgacgctg gcaatccggt ggatagttgg ggaaccggcc tcgtcgtcga ccacaatcac gtcatcacca acaaacatgt cgtgaccggg ctcgcgggca cgagcgcagg tctgtccgtc tatccgtcaa gcaaccacgc cgaagccgag ttggtgaact tctcgggcac cgctcacccg caccccacgc tcgacgtcgc agtaatcaaa ttcgagatgc ccgaaggcaa gtacatcccg cggcttggcg gcatggcgtt ccgtgatccc gactgggcgg atgaggtcta cgtgttcggt tacccgcggg tgccgatgac tgcggagatg gcgatcacgg tgcagcgtgg cgaggtggtc aacccggccg cgacgactat ccccggccgg cagaagattt tcctctactc ggcaatcgcg cggccgggca acagcggcgg tccgatcgtt gcgcaagacg gccgtgtgat cggcctcgtg gtcgaggact ctgcggaagc accgtcgacg ggcaccggac cgaacgcggc acccttctat cgcggcatcc cgtcgagcga agtgatccga gcactcgacg aactcgactt cggcggcatc gtggagatgg acaccctccc ataaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa