SSGCID: Target Page

MytuD.17531.a

Putative uncharacterized protein

CENTER ID:	MytuD.17531.a
ORGANISM:	Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE:	Tuberculosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MytuD.17531.a.A1.GE30993	Full length( MytuD.17531.a )		1	251

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|83332.12.peg.409
RefSeq:	NP_214886.1
TubercuList:	Rv0372c
MTB Network Portal:	Rv0372c
UniProt:	O53707

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSISDRAAQL VAARTPFVRA TVVRAQQPTS ARPGDEAILL ADGTIEGFVG GHCAQNSVRK AAMGVLQAGE SVLLRVLPDG DVHFPEAPGA CVVVNPCLAG GSLEIFLTPQ LPAPLIQIYG ETPIADALIE LCGLLGYDAR RDTDPADTDA LPTAIVIASH GGPEAEIIRT ALDNGVGYVG LVASTVRGAS ILDSLDLSDA ERARVHTPVG LAIGAKTPAE IAVSIAAELI ATLRGGGPRG RKALADENGG A

NT Sequence

ATGTCCATCA GTGACCGGGC CGCACAGCTG GTGGCCGCGC GGACACCGTT CGTGCGCGCG ACCGTGGTGC GGGCCCAGCA GCCCACGTCG GCCCGCCCGG GCGATGAGGC AATCCTGTTG GCGGACGGCA CCATCGAGGG CTTTGTCGGC GGCCACTGCG CGCAGAACTC GGTCCGCAAG GCGGCGATGG GCGTGTTGCA GGCCGGCGAG AGCGTGCTGC TGCGCGTGCT GCCCGACGGT GACGTGCACT TCCCGGAAGC TCCCGGGGCG TGTGTGGTGG TCAACCCGTG CCTGGCCGGC GGATCCCTGG AGATCTTCCT GACCCCGCAG CTGCCGGCCC CGCTGATCCA GATTTACGGC GAAACCCCGA TCGCCGACGC GTTGATCGAA CTGTGCGGCC TGTTGGGCTA CGACGCGCGC CGCGACACCG ATCCGGCCGA CACCGACGCC TTGCCAACTG CGATTGTGAT CGCCAGCCAC GGCGGGCCCG AAGCCGAAAT CATCCGCACG GCGCTGGATA ACGGCGTTGG GTATGTCGGG CTGGTGGCCA GCACGGTACG CGGCGCGTCC ATCCTGGATT CGCTGGACCT GTCCGATGCC GAGCGGGCCC GGGTGCATAC CCCGGTCGGA TTGGCCATCG GCGCCAAAAC TCCGGCGGAG ATCGCGGTGT CGATTGCGGC GGAACTGATC GCCACGCTGC GCGGCGGCGG CCCCAGAGGC CGGAAGGCGT TGGCGGACGA GAACGGCGGC GCA

Details for MytuD.17531.a.A1.GE30993

HARVESTED ON:	12/14/2010
SEQUENCED ON:	12/20/2010
EXPECTED MW:	27kDa
OBSERVED MW:	27kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	92

Validated AA Sequence

Validated NT Sequence

ttattatgcg ccgccgttct cgtccgccaa cgccttccgg cctctggggc cgccgccgcg cagcgtggcg atcagttccg ccgcaatcga caccgcgatc tccgccggag ttttggcgcc gatggccaat ccgaccgggg tatgcacccg ggcccgctcg gcatcggaca ggtccagcga atccaggatg gacgcgccgc gtaccgtgct ggccaccagc ccgacatacc caacgccgtt atccagcgcc gtgcggatga tttcggcttc gggcccgccg tggctggcga tcacaatcgc agttggcaag gcgtcggtgt cggccggatc ggtgtcgcgg cgcgcgtcgt agcccaacag gccgcacagt tcgatcaacg cgtcggcgat cggggtttcg ccgtaaatct ggatcagcgg ggccggcagc tgcggggtca ggaagatctc cagggatccg ccggccaggc acgggttgac caccacacac gccccgggag cttccgggaa gtgcacgtca ccgtcgggca gcacgcgcag cagcacgctc tcgccggcct gcaacacgcc catcgccgcc ttgcggaccg agttctgcgc gcagtggccg ccgacaaagc cctcgatggt gccgtccgcc aacaggattg cctcatcgcc cgggcgggcc gacgtgggct gctgggcccg caccacggtc gcgcgcacga acggtgtccg cgcggccacc agctgtgcgg cccggtcact gatggacatc gaaccaggac cctgggtctg agcttccagg gtacc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMSISDRAAQ LVAARTPFVR ATVVRAQQPT SARPGDEAIL LADGTIEGFV GGHCAQNSVR KAAMGVLQAG ESVLLRVLPD GDVHFPEAPG ACVVVNPCLA GGSLEIFLTP QLPAPLIQIY GETPIADALI ELCGLLGYDA RRDTDPADTD ALPTAIVIAS HGGPEAEIIR TALDNGVGYV GLVASTVRGA SILDSLDLSD AERARVHTPV GLAIGAKTPA EIAVSIAAEL IATLRGGGPR GRKALADENG GA

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtccatcagt gaccgggccg cacagctggt ggccgcgcgg acaccgttcg tgcgcgcgac cgtggtgcgg gcccagcagc ccacgtcggc ccgcccgggc gatgaggcaa tcctgttggc ggacggcacc atcgagggct ttgtcggcgg ccactgcgcg cagaactcgg tccgcaaggc ggcgatgggc gtgttgcagg ccggcgagag cgtgctgctg cgcgtgctgc ccgacggtga cgtgcacttc ccggaagctc ccggggcgtg tgtggtggtc aacccgtgcc tggccggcgg atccctggag atcttcctga ccccgcagct gccggccccg ctgatccaga tttacggcga aaccccgatc gccgacgcgt tgatcgaact gtgcggcctg ttgggctacg acgcgcgccg cgacaccgat ccggccgaca ccgacgcctt gccaactgcg attgtgatcg ccagccacgg cgggcccgaa gccgaaatca tccgcacggc gctggataac ggcgttgggt atgtcgggct ggtggccagc acggtacgcg gcgcgtccat cctggattcg ctggacctgt ccgatgccga gcgggcccgg gtgcataccc cggtcggatt ggccatcggc gccaaaactc cggcggagat cgcggtgtcg attgcggcgg aactgatcgc cacgctgcgc ggcggcggcc ccagaggccg gaaggcgttg gcggacgaga acggcggcgc ataaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa