MytuD.17509.a

Putative uncharacterized protein

CENTER ID: MytuD.17509.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.17509.a.A1.GE30991 Full length( MytuD.17509.a ) 1 284
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.3045
RefSeq: NP_217250.1
TubercuList: Rv2734
MTB Network Portal: Rv2734
UniProt: I6YA42

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSDRSAIEWT GATWNPVTGC DRVSPGCDHC YAMTLAKRLK AMGSDKYQTD GDPRTSGPGF GVTIHPRSLD EPFRWRSPRT VFVNSMADLF HARVALWFIR EVFEVMRATP QHTYQILTKR SLRLRRLAHK LEWPSNVWMG VSVENVDAFR RIEDLRQVPA AVRFLSCEPL LGPLDGINLG SIDWVIAGGE SGPNFRPIDP QWVRHIRDTC TAADVPFFFK QWGGRTPKAF GRELDGRCWD EMPLIEIRNP DPRTTSRVHA DPMLATAPTE SAQRSNPGQL VRQR
NT Sequence
ATGTCGGATC GCTCAGCGAT CGAATGGACG GGGGCAACCT GGAACCCGGT CACCGGATGC GACCGTGTAT CGCCGGGATG TGACCACTGC TACGCAATGA CGTTAGCGAA GCGGCTAAAG GCGATGGGCT CCGACAAGTA TCAAACCGAT GGTGACCCCA GAACCTCCGG TCCGGGATTT GGCGTCACCA TCCATCCCCG CAGTCTTGAC GAGCCGTTCC GGTGGCGAAG CCCCCGCACA GTGTTCGTGA ACTCGATGGC GGACCTATTT CACGCCAGGG TGGCGCTCTG GTTCATTAGG GAAGTGTTCG AGGTGATGCG AGCCACACCA CAGCACACTT ACCAGATCTT GACCAAGCGC AGCCTGCGAC TGCGTCGCCT CGCTCACAAG CTGGAGTGGC CCTCGAACGT TTGGATGGGG GTGTCGGTGG AAAATGTCGA CGCCTTCCGC CGTATCGAGG ACCTACGACA GGTGCCCGCA GCAGTAAGGT TCCTCTCCTG CGAGCCATTA CTCGGGCCCC TGGACGGAAT AAATCTAGGT TCGATTGATT GGGTTATCGC CGGAGGCGAA TCTGGTCCAA ATTTCCGCCC GATCGATCCA CAATGGGTTC GCCATATTCG CGATACCTGT ACTGCCGCTG ATGTCCCATT CTTCTTCAAG CAATGGGGCG GTAGAACACC AAAGGCATTT GGACGTGAAC TCGACGGACG TTGTTGGGAT GAAATGCCGC TTATTGAGAT TAGAAACCCG GATCCTCGGA CCACCAGCCG CGTGCACGCG GATCCCATGT TGGCGACGGC GCCCACAGAA TCTGCCCAGC GTTCGAATCC TGGACAGCTA GTTCGCCAAC GC
Details for MytuD.17509.a.A1.GE30991
HARVESTED ON: 12/14/2010
SEQUENCED ON: 12/20/2010
EXPECTED MW: 34kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 98
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSDRSAIEW TGATWNPVTG CDRVSPGCDH CYAMTLAKRL KAMGSDKYQT DGDPRTSGPG FGVTIHPRSL DEPFRWRSPR TVFVNSMADL FHARVALWFI REVFEVMRAT PQHTYQILTK RSLRLRRLAH KLEWPSNVWM GVSVENVDAF RRIEDLRQVP AAVRFLSCEP LLGPLDGINL GSIDWVIAGG ESGPNFRPID PQWVRHIRDT CTAADVPFFF KQXXGRTPKA FGRELDGRCW DEMPLIEIRN PDPRTTSRVH ADPMLAXXPT ESAQRSNPGQ LVRQR
Validated NT Sequence
ttgtttnnct ttaagaagga gatataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgtcggat cgctcagcga tcgaatggac gggggcaacc tggaacccgg tcaccggatg cgaccgtgta tcgccgggat gtgaccactg ctacgcaatg acgttagcga agcggctaaa ggcgatgggc tccgacaagt atcaaaccga tggtgacccc agaacctccg gtccgggatt tggcgtcacc atccatcccc gcagtcttga cgagccgttc cggtggcgaa gcccccgcac agtgttcgtg aactcgatgg cggacctatt tcacgccagg gtggcgctct ggttcattag ggaagtgttc gaggtgatgc gagccacacc acagcacact taccagatct tgaccaagcg cagcctgcga ctgcgtcgcc tcgctcacaa gctggagtgg ccctcgaacg tttggatggg ggtgtcggtg gaaaatgtcg acgccttccg ccgtatcgag gacctacgac aggtgcccgc agcagtaagg ttcctctcct gcgagccatt actcgggccc ctggacggaa taaatctagg ttcgattgat tgggttatcg ccggaggcga atctggtcca aatttccgcc cgatcgatcc acaatgggtt cgccatattc gcgatacctg tactgccgct gatgtcccat tcttcttcaa gcaatgnngc ggtagaacac caaaggcatt tggacgtgaa ctcgacggac gttgttggga tgaaatgccg cttattgaga ttagaaaccc ggatcctcgg accaccagcc gcgtgcacgc ggatcccatg ttggcgangn cgcccacaga atctgcccag cgttcgaatc ctggncagct agttcgccaa cgctaataaa cagcacgaac aagttctgca gccaagctnc tcgaggatcn nnctgctaac aaagcccgaa aggnagctga nttggctgcn nnnnnnnnnn nnnnnnanaa aaaaa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSDRSAIEW TGATWNPVTG CDRVSPGCDH CYAMTLAKRL KAMGSDKYQT DGDPRTSGPG FGVTIHPRSL DEPFRWRSPR TVFVNSMADL FHARVALWFI REVFEVMRAT PQHTYQILTK RSLRLRRLAH KLEWPSNVWM GVSVENVDAF RRIEDLRQVP AAVRFLSCEP LLGPLDGINL GSIDWVIAGG ESGPNFRPID PQWVRHIRDT CTAADVPFFF KQWGGRTPKA FGRELDGRCW DEMPLIEIRN PDPRTTSRVH ADPMLATAPT ESAQRSNPGQ LVRQR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcggatcgc tcagcgatcg aatggacggg ggcaacctgg aacccggtca ccggatgcga ccgtgtatcg ccgggatgtg accactgcta cgcaatgacg ttagcgaagc ggctaaaggc gatgggctcc gacaagtatc aaaccgatgg tgaccccaga acctccggtc cgggatttgg cgtcaccatc catccccgca gtcttgacga gccgttccgg tggcgaagcc cccgcacagt gttcgtgaac tcgatggcgg acctatttca cgccagggtg gcgctctggt tcattaggga agtgttcgag gtgatgcgag ccacaccaca gcacacttac cagatcttga ccaagcgcag cctgcgactg cgtcgcctcg ctcacaagct ggagtggccc tcgaacgttt ggatgggggt gtcggtggaa aatgtcgacg ccttccgccg tatcgaggac ctacgacagg tgcccgcagc agtaaggttc ctctcctgcg agccattact cgggcccctg gacggaataa atctaggttc gattgattgg gttatcgccg gaggcgaatc tggtccaaat ttccgcccga tcgatccaca atgggttcgc catattcgcg atacctgtac tgccgctgat gtcccattct tcttcaagca atggggcggt agaacaccaa aggcatttgg acgtgaactc gacggacgtt gttgggatga aatgccgctt attgagatta gaaacccgga tcctcggacc accagccgcg tgcacgcgga tcccatgttg gcgacggcgc ccacagaatc tgcccagcgt tcgaatcctg gacagctagt tcgccaacgc taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa