MytuD.17486.a
Putative uncharacterized protein
| CENTER ID: | MytuD.17486.a |
| ORGANISM: | Mycobacterium tuberculosis H37Rv |
| ASSOCIATED DISEASE: | Tuberculosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MytuD.17486.a.A1.GE30990 | Full length( MytuD.17486.a ) | 1 | 115 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
5IRD
DEPOSITED: 3/13/2016
DETERMINATION: NMR
CLONE: MytuD.17486.a.A1.GE30990
PROTEIN: MytuD.17486.a.A1.PB00072
Publications by SSGCID
A phenotarget approach for identifying an alkaloid interacting with the tuberculosis protein Rv1466
Buchko GW, Myler PJ, Quinn RJ, Xie Y, Feng Y, Mak T, Liu M, Di Capua A
Mar Drugs - 2020
volume 18, issue 3, pages 149
PMID: 32150903; PMCID: PMC7143284
PDB:
5IRD
Discovery of a natural product that binds to the Mycobacterium tuberculosis protein Rv1466 using native mass spectrometry
Buchko GW, Myler PJ, Quinn RJ, Feng YR, Laureanti J, Mak T, Liu M, Elnaas A, Grice D, Han J, Cook G, Di Capua A
Molecules - 2020
volume 25, issue 10, pages 2385
PMID: 32455540; PMCID: PMC7288112
PDB:
5IRD
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_132611 |
| PATRIC ID: | fig|83332.12.peg.1637 |
| RefSeq: | NP_215982.1 |
| TubercuList: | Rv1466 |
| MTB Network Portal: | Rv1466 |
| UniProt: | O53157 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSETSAPAEE LLADVEEAMR DVVDPELGIN VVDLGLVYGL DVQDGDEGTV ALIDMTLTSA ACPLTDVIED QSRSALVGSG LVDDIRINWV WNPPWGPDKI TEDGREQLRA LGFTV
NT Sequence
ATGAGCGAAA CCAGCGCACC GGCTGAGGAA TTGCTCGCCG ACGTCGAGGA GGCGATGCGC GACGTCGTCG ACCCGGAGCT GGGGATCAAC GTCGTTGACC TGGGCCTGGT CTACGGCTTG GACGTGCAAG ACGGTGACGA AGGGACCGTC GCGCTGATCG ACATGACCCT CACGTCGGCG GCGTGCCCGC TGACCGATGT CATCGAGGAT CAGTCGCGCA GCGCGCTGGT CGGCAGTGGC CTGGTCGACG ACATCCGCAT CAACTGGGTG TGGAACCCGC CGTGGGGCCC GGACAAGATC ACCGAAGACG GCCGCGAACA ATTGCGGGCG CTCGGCTTCA CCGTC
Details for MytuD.17486.a.A1.GE30990
| HARVESTED ON: | 12/14/2010 |
| SEQUENCED ON: | 12/20/2010 |
| EXPECTED MW: | 14kDa |
| OBSERVED MW: | 21kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSETSAPAE ELLADVEEAM RDVVDPELGI NVVDLGLVYG LDVQDGDEGT VALIDMTLTS AACPLTDVIE DQSRSALVGS GLVDDIRINW VWNPPWGPDK ITEDGREQLR ALG
Validated NT Sequence
aagccgagcg cccgcaattg ttcgcggccg tcttcggtga tcttgtccgg gccccacggc gggttccaca cccagttgat gcggatgtcg tcgaccaggc cactgccgac cagcgcgctg cgcgactgat cctcgatgac atcggtcagc gggcacgccg ccgacgtgag ggtcatgtcg atcagcgcga cggtcccttc gtcaccgtct tgcacgtcca agccgtagac caggcccagg tcaacgacgt tgatccccag ctccgggtcg acgacgtcgc gcatcgcctc ctcgacgtcg gcgagcaatt cctcagccgg tgcgctggtt tcgctcatcg aaccaggacc ctgggtctga gcttccaggg tacccatatg atggtgatgg tgatgagcca t
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSETSAPAE ELLADVEEAM RDVVDPELGI NVVDLGLVYG LDVQDGDEGT VALIDMTLTS AACPLTDVIE DQSRSALVGS GLVDDIRINW VWNPPWGPDK ITEDGREQLR ALGFTV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcgaaacc agcgcaccgg ctgaggaatt gctcgccgac gtcgaggagg cgatgcgcga cgtcgtcgac ccggagctgg ggatcaacgt cgttgacctg ggcctggtct acggcttgga cgtgcaagac ggtgacgaag ggaccgtcgc gctgatcgac atgaccctca cgtcggcggc gtgcccgctg accgatgtca tcgaggatca gtcgcgcagc gcgctggtcg gcagtggcct ggtcgacgac atccgcatca actgggtgtg gaacccgccg tggggcccgg acaagatcac cgaagacggc cgcgaacaat tgcgggcgct cggcttcacc gtctaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa