SSGCID: Target Page

MytuD.17479.a

Putative uncharacterized protein

CENTER ID:	MytuD.17479.a
ORGANISM:	Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE:	Tuberculosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MytuD.17479.a.A1.GE30989	Full length( MytuD.17479.a )		1	275

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|83332.12.peg.4012
RefSeq:	NP_218111.1
TubercuList:	Rv3594
MTB Network Portal:	Rv3594
UniProt:	I6Y3Z2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MGWIGDPIWL EEVLRPALGE RLRVLDGWRE RGHGDFRDIR GVMWHHTGNS RETAKSIARG RPDLPGPLAN LHIAHSGVVT IVAVGVCWHA GRGSYPWLPT DNANWHMIGV ECAWPTIRRD GSYDAGERWP DAQIVSMRDV AAALTLKLGY GPERNIGHKE YAGAAQGKWD PGNLSMDWFR AEVAKDTRGE FDHPLTPPPA VIARPPILPK PRNPRDDRIL LEEVWDQLRG IEGRGWPVLG DKTIVDYLAE LGNKVDALAA KLDAREGLDR PSDTR

NT Sequence

GTGGGATGGA TCGGCGATCC GATTTGGCTC GAGGAGGTGC TACGGCCGGC ACTCGGCGAG CGCCTGCGGG TGCTCGACGG CTGGCGGGAA CGCGGACACG GCGACTTTCG CGATATCCGC GGTGTGATGT GGCACCACAC CGGCAACTCA CGTGAGACCG CCAAAAGCAT TGCCCGCGGC CGGCCCGACT TACCCGGCCC GCTGGCCAAT CTGCACATCG CGCACAGCGG GGTCGTAACG ATCGTCGCGG TAGGCGTGTG CTGGCACGCC GGCCGCGGCA GCTACCCGTG GCTGCCAACC GACAACGCCA ACTGGCACAT GATTGGCGTC GAGTGCGCGT GGCCGACCAT CCGGCGTGAC GGCTCCTACG ACGCCGGTGA GCGCTGGCCT GACGCGCAGA TCGTGAGCAT GCGAGACGTC GCCGCGGCGC TCACGCTCAA GCTCGGCTAC GGGCCCGAAC GCAATATTGG GCACAAAGAG TATGCCGGGG CGGCTCAAGG CAAATGGGAC CCGGGAAACC TGTCGATGGA CTGGTTTCGC GCCGAGGTGG CAAAGGACAC GCGGGGCGAG TTCGACCACC CCCTCACCCC GCCGCCGGCG GTGATTGCCC GCCCACCGAT TCTGCCCAAG CCGCGCAACC CGCGTGACGA TCGCATCCTG CTCGAGGAGG TGTGGGACCA GCTACGCGGC ATCGAGGGCC GCGGCTGGCC GGTACTCGGC GACAAGACGA TCGTCGACTA CCTAGCCGAG CTCGGCAATA AGGTCGACGC CCTGGCCGCA AAACTCGACG CGCGCGAGGG CCTCGACCGG CCCAGTGACA CTCGG

Details for MytuD.17479.a.A1.GE30989

HARVESTED ON:	12/14/2010
SEQUENCED ON:	12/20/2010
EXPECTED MW:	33kDa
OBSERVED MW:	33kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	98
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMVGWIGDPI WLEEVLRPAL GERLRVLDGW RERGHGDFRD IRGVMWHHTG NSRETAKSIA RGRPDLPGPL ANLHIAHSGV VTIVAVGVCW HAGRGSYPWL PTDNANWHMI GVECAWPTIR RDGSYDAGER WPDAQIVSMR DVAAALTLKL GYGPERNIGH KEYAGAAQGK WDPGNLSMDW FRAEVAKDTR GEFDHPLTPP PAVIARPPIL PKPRNPRDDR ILLEEVWDQL RGIEGRGWPV LGDKTIVDYL AELGNKVDAL AAKLDAREGP RPAQ

Validated NT Sequence

ttttgttnnn tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatggtggg atggatcggc gatccgattt ggctcgagga ggtgctacgg ccggcactcg gcgagcgcct gcgggtgctc gacggctggc gggaacgcgg acacggcgac tttcgcgata tccgcggtgt gatgtggcac cacaccggca actcacgtga gaccgccaaa agcattgccc gcggccggcc cgacttaccc ggcccgctgg ccaatctgca catcgcgcac agcggggtcg taacgatcgt cgcggtaggc gtgtgctggc acgccggccg cggcagctac ccgtggctgc caaccgacaa cgccaactgg cacatgattg gcgtcgagtg cgcgtggccg accatccggc gtgacggctc ctacgacgcc ggtgagcgct ggcctgacgc gcagatcgtg agcatgcgag acgtcgccgc ggcgctcacg ctcaagctcg gctacgggcc cgaacgcaat attgggcaca aagagtatgc cggggcggct caaggcaaat gggacccggg aaacctgtcg atggactggt ttcgcgccga ggtggcaaag gacacgcggg gcgagttcga ccaccccctc accccgccgc cggcggtgat tgcccgccca ccgattctgc ccaagccgcg caacccgcgt gacgatcgca tcctgctcga ggaggtgtgg gaccagctac gcggcatcga gggccgcggc tggccggtac tcggcgacaa gacgatcgtc gactacctag ccgagctcgg caataaggtc gacgccctgg ccgcaaaact cgacgcgcgc gaggggcctc gaccggccca gtgacactcg gtaataaaca gcacgaacaa gttctgcagc cnagcttctc gaggatccnn ctgctaacaa agcccgaaag gnagctnagt nggctgctgc aa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgggatgg atcggcgatc cgatttggct cgaggaggtg ctacggccgg cactcggcga gcgcctgcgg gtgctcgacg gctggcggga acgcggacac ggcgactttc gcgatatccg cggtgtgatg tggcaccaca ccggcaactc acgtgagacc gccaaaagca ttgcccgcgg ccggcccgac ttacccggcc cgctggccaa tctgcacatc gcgcacagcg gggtcgtaac gatcgtcgcg gtaggcgtgt gctggcacgc cggccgcggc agctacccgt ggctgccaac cgacaacgcc aactggcaca tgattggcgt cgagtgcgcg tggccgacca tccggcgtga cggctcctac gacgccggtg agcgctggcc tgacgcgcag atcgtgagca tgcgagacgt cgccgcggcg ctcacgctca agctcggcta cgggcccgaa cgcaatattg ggcacaaaga gtatgccggg gcggctcaag gcaaatggga cccgggaaac ctgtcgatgg actggtttcg cgccgaggtg gcaaaggaca cgcggggcga gttcgaccac cccctcaccc cgccgccggc ggtgattgcc cgcccaccga ttctgcccaa gccgcgcaac ccgcgtgacg atcgcatcct gctcgaggag gtgtgggacc agctacgcgg catcgagggc cgcggctggc cggtactcgg cgacaagacg atcgtcgact acctagccga gctcggcaat aaggtcgacg ccctggccgc aaaactcgac gcgcgcgagg gcctcgaccg gcccagtgac actcggtaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa