MytuD.17470.a

Putative NAD(P)H nitroreductase RV3131/MT3217

CENTER ID: MytuD.17470.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.17470.a.B1.GE37759 full length 1 332
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.3496
RefSeq: NP_217647.3
RefSeq: YP_006516593.1
TubercuList: Rv3131
MTB Network Portal: Rv3131
UniProt: P9WIZ7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNTHFPDAET VRTVLTLAVR APSIHNTQPW RWRVCPTSLE LFSRPDMQLR STDPDGRELI LSCGVALHHC VVALASLGWQ AKVNRFPDPK DRCHLATIGV QPLVPDQADV ALAAAIPRRR TDRRAYSCWP VPGGDIALMA ARAARGGVML RQVSALDRMK AIVAQAVLDH VTDEEYLREL TIWSGRYGSV AGVPARNEPP SDPSAPIPGR LFAGPGLSQP SDVLPADDGA AILALGTETD DRLARLRAGE AASIVLLTAT AMGLACCPIT EPLEIAKTRD AVRAEVFGAG GYPQMLLRVG WAPINADPLP PTPRRELSQV VEWPEELLRQ RC
NT Sequence
atgaacaccc atttcccgga cgccgaaacc gtgcgaacgg ttctcaccct ggccgtccgg gccccctcca tccacaacac gcagccgtgg cggtggcggg tatgcccgac gagtctggag ctgttctcta gacccgatat gcagctgcgt agcaccgatc cggacgggcg tgagttgatc ctcagctgtg gtgtggcatt gcaccactgc gtcgtcgctt tggcgtcgct gggctggcag gccaaggtaa accgtttccc cgatcccaag gaccgctgcc atctggccac catcggggta caaccgcttg ttcccgatca ggccgatgtc gccttggcgg cggccatacc gcggcgacgc accgatcggc gcgcctacag ttgctggccg gtgccaggag gtgacatcgc gttgatggcc gcaagagcag cccgtggcgg ggtcatgctg cggcaggtca gtgccctaga ccgaatgaaa gccattgtgg cgcaggctgt cttggaccac gtgaccgacg aggaatatct gcgcgagctc accatttgga gtgggcgcta cggttcagtg gccggggttc ccgcccgcaa cgagccgcca tcagacccca gtgccccgat ccccggtcgc ctgttcgccg ggcccggtct gtctcagccg tccgacgtct tacccgctga cgacggcgcc gcgatcctgg cactaggcac cgagacagac gaccggttgg cccggctgcg cgccggcgag gccgccagca tcgtcttgtt gaccgcgacg gcaatggggc tggcgtgctg cccgatcacc gaaccgctgg agatcgccaa gacccgcgac gcggtccgtg ccgaggtgtt cggcgccggc ggctaccccc agatgctgct gcgagtgggt tgggcaccga tcaatgccga cccgttgcca ccgacgccac ggcgcgaact gtcccaggtc gttgagtggc cggaagagct actgcgacaa cggtgctga
Details for MytuD.17470.a.B1.GE37759
HARVESTED ON: 2/5/2014
SEQUENCED ON: 2/6/2014
EXPECTED MW: 37kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMN THFPDAETVR TVLTLAVRAP SIHNTQPWRW RVCPTSLELF SRPDMQLRST DPDGRELILS CGVALHHCVV ALASLGWQAK VNRFPDPKDR CHLATIGVQP LVPDQADVAL AAAIPRRRTD RRAYSCWPVP GGDIALMAAR AARGGVMLRQ VSALDRMKAI VAQAVLDHVT DEEYLRELTI WSGRYGSVAG VPARNEPPSD PSAPIPGRLF AGPGLSQPSD VLPADDGAAI LALGTETDDR LARLRAGEAA SIVLLTATAM GLACCPITEP LEIAKTRDAV RAEVFGAGGY PQMLLRVGWA PINADPLPPT PRRELSQVVE WPEELLRQRC
Validated NT Sequence
cttagcaccg ttgtcgcagt agctcttccg gccactcaac gacctgggac agttcgcgcc gtggcgtcgg tggcaacggg tcggcattga tcggtgccca acccactcgc agcagcatct gggggtagcc gccggcgccg aacacctcgg cacggaccgc gtcgcgggtc ttggcgatct ccagcggttc ggtgatcggg cagcacgcca gccccattgc cgtcgcggtc aacaagacga tgctggcggc ctcgccggcg cgcagccggg ccaaccggtc gtctgtctcg gtgcctagtg ccaggatcgc ggcgccgtcg tcagcgggta agacgtcgga cggctgagac agaccgggcc cggcgaacag gcgaccgggg atcggggcac tggggtctga tggcggctcg ttgcgggcgg gaaccccggc cactgaaccg tagcgcccac tccaaatggt gagctcgcgc agatattcct cgtcggtcac gtggtccaag acagcctgcg ccacaatggc tttcattcgg tctagggcac tgacctgccg cagcatgacc ccgccacggg ctgctcttgc ggccatcaac gcgatgtcac ctcctggcac cggccagcaa ctgtaggcgc gccgatcggt gcgtcgccgc ggtatggccg ccgccaaggc gacatcggcc tgatcgggaa caagcggttg taccccgatg gtggccagat ggcagcggtc cttgggatcg gggaaacggt ttaccttggc ctgccagccc agcgacgcca aagcgacgac gcagtggtgc aatgccacac cacagctgag gatcaactca cgcccgtccg gatcggtgct acgcagctgc atatcgggtc tagagaacag ctccagactc gtcgggcata cccgccaccg ccacggctgc gtgttgtgga tggagggggc ccggacggcc agggtgagaa ccgttcgcac ggtttcggcg tccgggaaat gggtgttcat atggtggtgg tggtggtgag ccat
Expected Protein Sequence
MAHHHHHHMN THFPDAETVR TVLTLAVRAP SIHNTQPWRW RVCPTSLELF SRPDMQLRST DPDGRELILS CGVALHHCVV ALASLGWQAK VNRFPDPKDR CHLATIGVQP LVPDQADVAL AAAIPRRRTD RRAYSCWPVP GGDIALMAAR AARGGVMLRQ VSALDRMKAI VAQAVLDHVT DEEYLRELTI WSGRYGSVAG VPARNEPPSD PSAPIPGRLF AGPGLSQPSD VLPADDGAAI LALGTETDDR LARLRAGEAA SIVLLTATAM GLACCPITEP LEIAKTRDAV RAEVFGAGGY PQMLLRVGWA PINADPLPPT PRRELSQVVE WPEELLRQRC
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaca cccatttccc ggacgccgaa accgtgcgaa cggttctcac cctggccgtc cgggccccct ccatccacaa cacgcagccg tggcggtggc gggtatgccc gacgagtctg gagctgttct ctagacccga tatgcagctg cgtagcaccg atccggacgg gcgtgagttg atcctcagct gtggtgtggc attgcaccac tgcgtcgtcg ctttggcgtc gctgggctgg caggccaagg taaaccgttt ccccgatccc aaggaccgct gccatctggc caccatcggg gtacaaccgc ttgttcccga tcaggccgat gtcgccttgg cggcggccat accgcggcga cgcaccgatc ggcgcgccta cagttgctgg ccggtgccag gaggtgacat cgcgttgatg gccgcaagag cagcccgtgg cggggtcatg ctgcggcagg tcagtgccct agaccgaatg aaagccattg tggcgcaggc tgtcttggac cacgtgaccg acgaggaata tctgcgcgag ctcaccattt ggagtgggcg ctacggttca gtggccgggg ttcccgcccg caacgagccg ccatcagacc ccagtgcccc gatccccggt cgcctgttcg ccgggcccgg tctgtctcag ccgtccgacg tcttacccgc tgacgacggc gccgcgatcc tggcactagg caccgagaca gacgaccggt tggcccggct gcgcgccggc gaggccgcca gcatcgtctt gttgaccgcg acggcaatgg ggctggcgtg ctgcccgatc accgaaccgc tggagatcgc caagacccgc gacgcggtcc gtgccgaggt gttcggcgcc ggcggctacc cccagatgct gctgcgagtg ggttgggcac cgatcaatgc cgacccgttg ccaccgacgc cacggcgcga actgtcccag gtcgttgagt ggccggaaga gctactgcga caacggtgct gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat