MytuD.17446.a

Putative uncharacterized protein

CENTER ID: MytuD.17446.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.17446.a.A1.GE30984 Full length( MytuD.17446.a ) 1 242
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.17446.a.A1.PW35471 Full length( MytuD.17446.a ) 1 242

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.813
RefSeq: NP_215244.1
TubercuList: Rv0730
MTB Network Portal: Rv0730
UniProt: I6XW38

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MHGARTGVSF YAYAMTDHDQ TAARREIADA LLAALERRHE VADAIVEAAN KAAAVEAIVN LLGTSHLAAE AVMSMSFDQL TQDARTKIIA ELDDLNKQLS FTVKERPASS GEGLELRPFS PDEDRDIFAR RTEEMGAAGD GSGGPAGSVD DEIRAAQKRV DDEEAAWFVA VDSGVKVGMV FGELVHGEVD VRIWIHPDHR KKGYGTAALR KSRSEMAWAF PAVPMVARAP AAQPAQPGSA GR
NT Sequence
ATGCATGGCG CAAGAACTGG TGTGTCGTTC TACGCTTATG CAATGACAGA TCACGACCAG ACCGCGGCCC GTCGAGAGAT CGCCGATGCC CTGCTCGCCG CGCTGGAACG TCGGCATGAG GTCGCAGACG CCATCGTGGA GGCCGCCAAC AAGGCCGCCG CCGTCGAGGC GATCGTGAAC TTGCTGGGCA CCTCGCACTT GGCCGCCGAA GCGGTGATGA GCATGTCTTT CGATCAGCTC ACCCAGGATG CGCGCACAAA GATCATCGCC GAGCTCGACG ACCTGAACAA ACAGCTGAGC TTCACCGTCA AGGAGCGTCC AGCCAGCTCT GGTGAGGGCC TGGAGCTGCG GCCGTTCTCC CCAGATGAGG ACCGCGACAT CTTCGCTCGA CGAACCGAAG AAATGGGCGC CGCCGGCGAT GGATCCGGGG GACCCGCCGG CAGCGTCGAC GACGAGATCC GAGCCGCACA GAAGCGCGTC GACGACGAGG AGGCGGCTTG GTTCGTGGCT GTTGATTCCG GCGTCAAGGT CGGGATGGTG TTCGGCGAGC TTGTCCACGG CGAGGTGGAC GTCCGGATCT GGATTCACCC CGATCATCGA AAAAAGGGTT ACGGAACCGC GGCATTGCGC AAGTCGCGCT CGGAGATGGC CTGGGCGTTC CCGGCCGTGC CGATGGTCGC CCGCGCGCCC GCGGCCCAAC CCGCCCAGCC GGGAAGTGCC GGCCGG
Details for MytuD.17446.a.A1.GE30984
HARVESTED ON: 12/14/2010
SEQUENCED ON: 12/20/2010
EXPECTED MW: 28kDa
OBSERVED MW: 28kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMHGARTGVS FYAYAMTDHD QTAARREIAD ALLAALERRH EVADAIVEAA NKAAAVEAIV NLLGTSHLAA EAVMSMSFDQ LTQDARTKII AELDDLNKQL SFTVKERPAS SGEGLELRPF SPDEDRDIFA RRTEEMGAAG DGSGGPAGSV DDEIRAAQKR VDDEEAAWFV AVDSGVKVGM VFGELVHGEV DVRIWIHPDH RKKGYGTAAL RKSRSEMAWA FPAVPMVARA PAAQPAQPGS AGR
Validated NT Sequence
ttattaccgg ccggcacttc ccggctgggc gggttgggcc gcgggcgcgc gggcgaccat cggcacggcc gggaacgccc aggccatctc cgagcgcgac ttgcgcaatg ccgcggttcc gtaacccttt tttcgatgat cggggtgaat ccagatccgg acgtccacct cgccgtggac aagctcgccg aacaccatcc cgaccttgac gccggaatca acagccacga accaagccgc ctcctcgtcg tcgacgcgct tctgtgcggc tcggatctcg tcgtcgacgc tgccggcggg tcccccggat ccatcgccgg cggcgcccat ttcttcggtt cgtcgagcga agatgtcgcg gtcctcatct ggggagaacg gccgcagctc caggccctca ccagagctgg ctggacgctc cttgacggtg aagctcagct gtttgttcag gtcgtcgagc tcggcgatga tctttgtgcg cgcatcctgg gtgagctgat cgaaagacat gctcatcacc gcttcggcgg ccaagtgcga ggtgcccagc aagttcacga tcgcctcgac ggcggcggcc ttgttggcgg cctccacgat ggcgtctgcg acctcatgcc gacgttccag cgcggcgagc agggcatcgg cgatctctcg acgggccgcg gtctggtcgt gatctgtcat tgcataagcg tagaacgaca caccagttct tgcgccatgc atcgaaccag gaccctgggt ctgagcttcc agggtaccca tatgatggtg atggtgatga gccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMHGARTGVS FYAYAMTDHD QTAARREIAD ALLAALERRH EVADAIVEAA NKAAAVEAIV NLLGTSHLAA EAVMSMSFDQ LTQDARTKII AELDDLNKQL SFTVKERPAS SGEGLELRPF SPDEDRDIFA RRTEEMGAAG DGSGGPAGSV DDEIRAAQKR VDDEEAAWFV AVDSGVKVGM VFGELVHGEV DVRIWIHPDH RKKGYGTAAL RKSRSEMAWA FPAVPMVARA PAAQPAQPGS AGR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcatggcgca agaactggtg tgtcgttcta cgcttatgca atgacagatc acgaccagac cgcggcccgt cgagagatcg ccgatgccct gctcgccgcg ctggaacgtc ggcatgaggt cgcagacgcc atcgtggagg ccgccaacaa ggccgccgcc gtcgaggcga tcgtgaactt gctgggcacc tcgcacttgg ccgccgaagc ggtgatgagc atgtctttcg atcagctcac ccaggatgcg cgcacaaaga tcatcgccga gctcgacgac ctgaacaaac agctgagctt caccgtcaag gagcgtccag ccagctctgg tgagggcctg gagctgcggc cgttctcccc agatgaggac cgcgacatct tcgctcgacg aaccgaagaa atgggcgccg ccggcgatgg atccggggga cccgccggca gcgtcgacga cgagatccga gccgcacaga agcgcgtcga cgacgaggag gcggcttggt tcgtggctgt tgattccggc gtcaaggtcg ggatggtgtt cggcgagctt gtccacggcg aggtggacgt ccggatctgg attcaccccg atcatcgaaa aaagggttac ggaaccgcgg cattgcgcaa gtcgcgctcg gagatggcct gggcgttccc ggccgtgccg atggtcgccc gcgcgcccgc ggcccaaccc gcccagccgg gaagtgccgg ccggtaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for MytuD.17446.a.A1.PW35471
PURIFICATION DATe: 1/14/2012
CONCENTRATION: 34mg/ml
OBSERVED MW: 30kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 1
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMHGARTGVS FYAYAMTDHD QTAARREIAD ALLAALERRH EVADAIVEAA NKAAAVEAIV NLLGTSHLAA EAVMSMSFDQ LTQDARTKII AELDDLNKQL SFTVKERPAS SGEGLELRPF SPDEDRDIFA RRTEEMGAAG DGSGGPAGSV DDEIRAAQKR VDDEEAAWFV AVDSGVKVGM VFGELVHGEV DVRIWIHPDH RKKGYGTAAL RKSRSEMAWA FPAVPMVARA PAAQPAQPGS AGR
Validated NT Sequence
ttattaccgg ccggcacttc ccggctgggc gggttgggcc gcgggcgcgc gggcgaccat cggcacggcc gggaacgccc aggccatctc cgagcgcgac ttgcgcaatg ccgcggttcc gtaacccttt tttcgatgat cggggtgaat ccagatccgg acgtccacct cgccgtggac aagctcgccg aacaccatcc cgaccttgac gccggaatca acagccacga accaagccgc ctcctcgtcg tcgacgcgct tctgtgcggc tcggatctcg tcgtcgacgc tgccggcggg tcccccggat ccatcgccgg cggcgcccat ttcttcggtt cgtcgagcga agatgtcgcg gtcctcatct ggggagaacg gccgcagctc caggccctca ccagagctgg ctggacgctc cttgacggtg aagctcagct gtttgttcag gtcgtcgagc tcggcgatga tctttgtgcg cgcatcctgg gtgagctgat cgaaagacat gctcatcacc gcttcggcgg ccaagtgcga ggtgcccagc aagttcacga tcgcctcgac ggcggcggcc ttgttggcgg cctccacgat ggcgtctgcg acctcatgcc gacgttccag cgcggcgagc agggcatcgg cgatctctcg acgggccgcg gtctggtcgt gatctgtcat tgcataagcg tagaacgaca caccagttct tgcgccatgc atcgaaccag gaccctgggt ctgagcttcc agggtaccca tatgatggtg atggtgatga gccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMHGARTGVS FYAYAMTDHD QTAARREIAD ALLAALERRH EVADAIVEAA NKAAAVEAIV NLLGTSHLAA EAVMSMSFDQ LTQDARTKII AELDDLNKQL SFTVKERPAS SGEGLELRPF SPDEDRDIFA RRTEEMGAAG DGSGGPAGSV DDEIRAAQKR VDDEEAAWFV AVDSGVKVGM VFGELVHGEV DVRIWIHPDH RKKGYGTAAL RKSRSEMAWA FPAVPMVARA PAAQPAQPGS AGR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCATGGCGCA AGAACTGGTG TGTCGTTCTA CGCTTATGCA ATGACAGATC ACGACCAGAC CGCGGCCCGT CGAGAGATCG CCGATGCCCT GCTCGCCGCG CTGGAACGTC GGCATGAGGT CGCAGACGCC ATCGTGGAGG CCGCCAACAA GGCCGCCGCC GTCGAGGCGA TCGTGAACTT GCTGGGCACC TCGCACTTGG CCGCCGAAGC GGTGATGAGC ATGTCTTTCG ATCAGCTCAC CCAGGATGCG CGCACAAAGA TCATCGCCGA GCTCGACGAC CTGAACAAAC AGCTGAGCTT CACCGTCAAG GAGCGTCCAG CCAGCTCTGG TGAGGGCCTG GAGCTGCGGC CGTTCTCCCC AGATGAGGAC CGCGACATCT TCGCTCGACG AACCGAAGAA ATGGGCGCCG CCGGCGATGG ATCCGGGGGA CCCGCCGGCA GCGTCGACGA CGAGATCCGA GCCGCACAGA AGCGCGTCGA CGACGAGGAG GCGGCTTGGT TCGTGGCTGT TGATTCCGGC GTCAAGGTCG GGATGGTGTT CGGCGAGCTT GTCCACGGCG AGGTGGACGT CCGGATCTGG ATTCACCCCG ATCATCGAAA AAAGGGTTAC GGAACCGCGG CATTGCGCAA GTCGCGCTCG GAGATGGCCT GGGCGTTCCC GGCCGTGCCG ATGGTCGCCC GCGCGCCCGC GGCCCAACCC GCCCAGCCGG GAAGTGCCGG CCGGTAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA