MytuD.17417.a

Putative uncharacterized protein

CENTER ID: MytuD.17417.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.17417.a.A1.GE30982 Full length( MytuD.17417.a ) 1 335
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.4299
RefSeq: NP_218373.1
TubercuList: Rv3856c
MTB Network Portal: Rv3856c
UniProt: P96221

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDPVTALRQI AYYKDRNRHD PRRVMAYRNA ADIIEGLDDA ARQRHGQANS WQSLAGIGPK TAKVIAQAWS GREPDLLAEL RADAEDLGGG AIRAALRGDL HLHSNWSDGS APIEEMMATA AALGHQYCAL TDHSPRLTIA NGLSPDRLRK QLDVIDELRE KFAPLRILTG IEVDILEDGS LDQEPEMLDR LDIVVASVHS KLSMDSAAMT RRMVRAVANG HTDVLGHCTG RLIAGNRGIR PESKFDAEAV FTACREHGTA VEINSRPERR DPPTRLLHLA RDIGCVFSID TDAHAPGQLD FLGYGAQRAL DAEVPADRIV NTWPADTLLA WTGSH
NT Sequence
ATGGACCCGG TAACAGCGCT GCGGCAGATC GCCTATTACA AGGACCGGAA CCGCCACGAC CCGAGGCGGG TGATGGCCTA TCGCAATGCC GCCGACATTA TCGAGGGCCT TGACGACGCG GCGCGGCAGC GGCACGGCCA GGCCAACAGC TGGCAGTCGC TGGCGGGTAT CGGTCCCAAA ACCGCGAAAG TCATCGCCCA GGCCTGGTCT GGCCGCGAGC CGGACCTGCT GGCTGAATTA CGCGCTGACG CTGAGGATCT CGGTGGAGGG GCGATCCGTG CCGCATTGCG CGGGGATCTG CATCTGCATT CGAACTGGTC GGACGGGTCC GCGCCGATCG AGGAGATGAT GGCCACCGCG GCGGCGCTAG GTCACCAGTA CTGCGCATTG ACCGATCACT CACCGCGGTT GACGATCGCC AACGGCCTGT CTCCGGACCG GTTGCGCAAG CAGCTGGACG TGATCGACGA GCTGCGCGAG AAGTTCGCGC CGCTGCGCAT CCTGACCGGG ATCGAGGTCG ACATCCTCGA AGACGGGAGC TTGGACCAGG AACCCGAGAT GCTGGACCGC CTCGACATCG TGGTGGCCAG CGTGCACTCC AAGCTGTCGA TGGATTCGGC AGCGATGACG CGACGGATGG TGCGCGCCGT GGCCAACGGT CACACCGACG TGCTGGGCCA CTGCACCGGC CGGCTGATCG CCGGCAATCG TGGCATCCGG CCAGAATCGA AGTTCGATGC AGAGGCGGTG TTCACCGCCT GCCGTGAGCA CGGCACCGCC GTGGAGATCA ACTCCCGTCC GGAACGCCGA GACCCACCGA CGCGCCTGTT GCACCTAGCG CGCGACATCG GCTGCGTGTT CAGCATCGAC ACCGACGCGC ACGCACCCGG CCAGCTGGAC TTCCTCGGCT ACGGCGCCCA GCGCGCCTTG GACGCGGAGG TGCCCGCCGA CCGGATCGTC AACACCTGGC CGGCCGACAC GCTCCTGGCG TGGACCGGGT CGCAT
Details for MytuD.17417.a.A1.GE30982
HARVESTED ON: 12/14/2010
SEQUENCED ON: 12/20/2010
EXPECTED MW: 38kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 87
PERCENT COVERAGE: 108
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMDPVTALRQ IAYYKDRNRH DPRRVMAYRN AADIIEGLDD AARQRHGQAN SWQSLAGIGP KTAKVIAQAW SGREPDLLAE LRADAEDLGG GAIRAALRGD LHLHSNWSDG SAPIEEMMAT AAALGHQYCA LTDHSPRLTI ANGLSPDRLR KQLDVIDELR EKFAPLRILT GIEVDILEDG SLDQEPEMLD RLDIVVASVH SKLSMDSAAM TRRMVRAVXN GHTDVLGHCT GRLIAGNRGI RPESKFDAEA VFTAXREHGT AVXINSRPEX XDPPTRLLHL ARDIXCVFSI XXDAHAPXXW TSSAXXXSAX WXXPXXXIXX XXXXXXXXGX XXXXXXXXXX XXXXXXXXXX XXXLXXXXXX XXXXXXXX
Validated NT Sequence
ttntttnnct ttaagaagga ganataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatggacccg gtaacagcgc tgcggcagat cgcctattac aaggaccgga accgccacga cccgaggcgg gtgatggcct atcgcaatgc cgccgacatt atcgagggcc ttgacgacgc ggcgcggcag cggcacggcc aggccaacag ctggcagtcg ctggcgggta tcggtcccaa aaccgcgaaa gtcatcgccc aggcctggtc tggccgcgag ccggacctgc tggctgaatt acgcgctgac gctgaggatc tcggtggagg ggcgatccgt gccgcattgc gcggggatct gcatctgcat tcgaactggt cggacgggtc cgcgccgatc gaggagatga tggccaccgc ggcggcgcta ggtcaccagt actgcgcatt gaccgatcac tcaccgcggt tgacgatcgc caacggcctg tctccggacc ggttgcgcaa gcagctggac gtgatcgacg agctgcgcga gaagttcgcg ccgctgcgca tcctgaccgg gatcgaggtc gacatcctcg aagacgggag cttggaccag gaacccgaga tgctggaccg cctcgacatc gtggtggcca gcgtgcactc caagctgtcg atggattcgg cagcgatgac gcgacggatg gtgcgcgccg tgnccaacgg tcacaccgac gtgctgggcc actgcaccgg ccggctgatc gccggcaatc gtggcatccg gccagaatcg aagttcgatg cagaggcggt gttcaccgcc tgncgtgagc acggcaccgc cgtgnnnatc aactcncgtc cggaangccn agacccaccg acgcgcctgt tgcacctagc gcgcgacatc ngctgcgtgt tcagcatcga nnccgacgcg cacgcncccn nnngctggac ttcctcggct angnnnncca gcgcgncntg gnngnngccn nccgancnga tcgnnannnn cnggnnnnnn nannnnncnn cnggngnnnn nnnnnnannn ntaancannn nnnnnnntnt gcnnnnnntn nnnnnngntn nnnnnaannn nnnnnnnctn nnngnnnnnt nnnnnnnnnn nnnnnnnnnn aannnnnnnn nnnnt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDPVTALRQ IAYYKDRNRH DPRRVMAYRN AADIIEGLDD AARQRHGQAN SWQSLAGIGP KTAKVIAQAW SGREPDLLAE LRADAEDLGG GAIRAALRGD LHLHSNWSDG SAPIEEMMAT AAALGHQYCA LTDHSPRLTI ANGLSPDRLR KQLDVIDELR EKFAPLRILT GIEVDILEDG SLDQEPEMLD RLDIVVASVH SKLSMDSAAM TRRMVRAVAN GHTDVLGHCT GRLIAGNRGI RPESKFDAEA VFTACREHGT AVEINSRPER RDPPTRLLHL ARDIGCVFSI DTDAHAPGQL DFLGYGAQRA LDAEVPADRI VNTWPADTLL AWTGSH
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggacccggta acagcgctgc ggcagatcgc ctattacaag gaccggaacc gccacgaccc gaggcgggtg atggcctatc gcaatgccgc cgacattatc gagggccttg acgacgcggc gcggcagcgg cacggccagg ccaacagctg gcagtcgctg gcgggtatcg gtcccaaaac cgcgaaagtc atcgcccagg cctggtctgg ccgcgagccg gacctgctgg ctgaattacg cgctgacgct gaggatctcg gtggaggggc gatccgtgcc gcattgcgcg gggatctgca tctgcattcg aactggtcgg acgggtccgc gccgatcgag gagatgatgg ccaccgcggc ggcgctaggt caccagtact gcgcattgac cgatcactca ccgcggttga cgatcgccaa cggcctgtct ccggaccggt tgcgcaagca gctggacgtg atcgacgagc tgcgcgagaa gttcgcgccg ctgcgcatcc tgaccgggat cgaggtcgac atcctcgaag acgggagctt ggaccaggaa cccgagatgc tggaccgcct cgacatcgtg gtggccagcg tgcactccaa gctgtcgatg gattcggcag cgatgacgcg acggatggtg cgcgccgtgg ccaacggtca caccgacgtg ctgggccact gcaccggccg gctgatcgcc ggcaatcgtg gcatccggcc agaatcgaag ttcgatgcag aggcggtgtt caccgcctgc cgtgagcacg gcaccgccgt ggagatcaac tcccgtccgg aacgccgaga cccaccgacg cgcctgttgc acctagcgcg cgacatcggc tgcgtgttca gcatcgacac cgacgcgcac gcacccggcc agctggactt cctcggctac ggcgcccagc gcgccttgga cgcggaggtg cccgccgacc ggatcgtcaa cacctggccg gccgacacgc tcctggcgtg gaccgggtcg cattaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa