MytuD.17397.a

Putative uncharacterized protein

CENTER ID: MytuD.17397.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.17397.a.A1.GE30979 Full length( MytuD.17397.a ) 1 352
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.2394
RefSeq: NP_216659.1
TubercuList: Rv2143
MTB Network Portal: Rv2143
UniProt: O06232

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEAPPYAGDP TFERLRRSFQ PADLLPELQA AGVHYTIAVE AADDPAENES LLATARHHDW IARVIGWVPL ADPDEVTESS THGRHRPDAS WRRDLRCPGL LPPGCHQPVL VVGLVGQQPE MRPMNPPSGF LRRTPTRRFR DRRDAGRVLA DELASYRGRD RLLVLGLARG GVPVGWEVAS ALGAELDVFL VRKLGVPQWR ELAMGALASG GGVVMNDDVV SSLRITDQQV RAAIDSETAE LQRRELAYRG GRPVVDPRAR IVILVDDGIA TGASMLAAVR TIRATGPESI VVAVPVGPAT ACRELAAEAD DVVCATMPAA FEAVGQVYND FHQVTDDEVR ELLATPTTGA AT
NT Sequence
ATGGAAGCGC CACCATATGC CGGCGATCCG ACGTTCGAGC GTTTGCGGCG CTCGTTTCAG CCAGCCGATC TACTGCCGGA ACTGCAAGCG GCAGGAGTGC ATTACACAAT CGCTGTCGAG GCGGCGGACG ATCCGGCCGA GAATGAGTCT CTGTTGGCCA CTGCGCGCCA CCATGATTGG ATAGCGCGCG TGATCGGTTG GGTCCCACTC GCCGATCCGG ATGAGGTTAC CGAGAGCTCG ACGCACGGGC GGCACCGCCC GGACGCCTCC TGGCGACGAG ATCTGCGGTG CCCCGGCCTG CTGCCGCCCG GGTGCCACCA GCCAGTCTTG GTCGTAGGCT TGGTAGGTCA GCAGCCGGAA ATGCGACCGA TGAATCCACC AAGTGGTTTT CTCCGGCGGA CGCCGACCCG CAGGTTTCGC GACCGCCGCG ATGCTGGTCG CGTATTGGCC GACGAACTTG CGTCCTATCG CGGCAGGGAC CGGTTGCTCG TCCTCGGCCT TGCCCGCGGT GGCGTCCCCG TCGGCTGGGA AGTCGCGTCG GCGCTAGGCG CCGAATTGGA TGTATTTCTG GTTCGCAAGC TCGGCGTGCC GCAGTGGCGC GAGCTGGCGA TGGGCGCGTT GGCCAGTGGG GGCGGGGTCG TGATGAACGA CGACGTGGTT TCCAGCTTGC GCATCACCGA CCAGCAGGTG CGTGCGGCGA TCGACAGCGA GACGGCAGAG CTGCAGCGGC GCGAGCTGGC GTATCGCGGC GGACGCCCTG TCGTCGATCC GCGCGCCAGG ATCGTGATCC TGGTTGACGA CGGCATCGCC ACCGGCGCGA GCATGCTGGC GGCGGTGCGC ACCATCCGTG CCACCGGACC GGAGTCGATC GTCGTCGCGG TCCCGGTCGG TCCGGCCACA GCCTGCCGCG AGCTCGCGGC GGAAGCCGAC GACGTGGTGT GCGCAACCAT GCCGGCAGCG TTTGAGGCCG TCGGCCAGGT CTATAACGAC TTTCATCAGG TCACCGACGA CGAGGTCCGC GAGCTGCTCG CGACGCCAAC CACAGGCGCA GCGACC
Details for MytuD.17397.a.A1.GE30979
HARVESTED ON: 12/14/2010
SEQUENCED ON: 12/20/2010
EXPECTED MW: 40kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAXTQG PGSMEAPPYA GDPTFERLRR SFQPADLLPE LQAAGVHYTI AVEAADDPAE NESLLATARH HDWIARVIGW VPLADPDEVT ESSTHGRHRP DASWRRDLRC PGLLPPGCHQ PVLVVGLVGQ QPEMRPMNPP SGFLRRTPTR RFRDRRDAGR VLADELASYR GRDRLLVLGL ARGGVPVGWE VASALGAELD VFLVRKLGVP QWRELAMGAL ASGGGVVMND DVVSSLRITD QQVRAAIDSE TAELQRRELA YRGGRPVVDP RARIVILVDD GIATGASMLA AVRTIRATGP ESIVVAVPVG PATACRELAA EADDVVCATM PAAFEAVGQV YNDFHQVTDD EVRELLATPT TGAA
Validated NT Sequence
gtcgctgcgc ctgtggttgg cgtcgcgagc agctcgcgga cctcgtcgtc ggtgacctga tgaaagtcgt tatagacctg gccgacggcc tcaaacgctg ccggcatggt tgcgcacacc acgtcgtcgg cttccgccgc gagctcgcgg caggctgtgg ccggaccgac cgggaccgcg acgacgatcg actccggtcc ggtggcacgg atggtgcgca ccgccgccag catgctcgcg ccggtggcga tgccgtcgtc aaccaggatc acgatcctgg cgcgcggatc gacgacaggg cgtccgccgc gatacgccag ctcgcgccgc tgcagctctg ccgtctcgct gtcgatcgcc gcacgcacct gctggtcggt gatgcgcaag ctggaaacca cgtcgtcgtt catcacgacc ccgcccccac tggccaacgc gcccatcgcc agctcgcgcc actgcggcac gccgagcttg cgaaccagaa atacatccaa ttcggcgcct agcgccgacg cgacttccca gccgacgggg acgccaccgc gggcaaggcc gaggacgagc aaccggtccc tgccgcgata ggacgcaagt tcgtcggcca atacgcgacc agcatcgcgg cggtcgcgaa acctgcgggt cggcgtccgc cggagaaaac cacttggtgg attcatcggt cgcatttccg gctgctgacc taccaagcct acgaccaaga ctggctggtg gcacccgggc ggcagcaggc cggggcaccg cagatctcgt cgccaggagg cgtccgggcg gtgccgcccg tgcgtcgagc tctcggtaac ctcatccgga tcggcgagtg ggacccaacc gatcacgcgc gctatccaat catggtggcg cgcagtggcc aacagagact cattctcggc cggatcgtcc gccgcctcga cagcgattgt gtaatgcact cctgccgctt gcagttccgg cagtagatcg gctggctgaa acgagcgccg caaacgctcg aacgtcggat cgccggcata tggtggcgct tccatcgaac caggaccctg ggtntgagct tccagggtac ccatat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMEAPPYAGD PTFERLRRSF QPADLLPELQ AAGVHYTIAV EAADDPAENE SLLATARHHD WIARVIGWVP LADPDEVTES STHGRHRPDA SWRRDLRCPG LLPPGCHQPV LVVGLVGQQP EMRPMNPPSG FLRRTPTRRF RDRRDAGRVL ADELASYRGR DRLLVLGLAR GGVPVGWEVA SALGAELDVF LVRKLGVPQW RELAMGALAS GGGVVMNDDV VSSLRITDQQ VRAAIDSETA ELQRRELAYR GGRPVVDPRA RIVILVDDGI ATGASMLAAV RTIRATGPES IVVAVPVGPA TACRELAAEA DDVVCATMPA AFEAVGQVYN DFHQVTDDEV RELLATPTTG AAT
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggaagcgcca ccatatgccg gcgatccgac gttcgagcgt ttgcggcgct cgtttcagcc agccgatcta ctgccggaac tgcaagcggc aggagtgcat tacacaatcg ctgtcgaggc ggcggacgat ccggccgaga atgagtctct gttggccact gcgcgccacc atgattggat agcgcgcgtg atcggttggg tcccactcgc cgatccggat gaggttaccg agagctcgac gcacgggcgg caccgcccgg acgcctcctg gcgacgagat ctgcggtgcc ccggcctgct gccgcccggg tgccaccagc cagtcttggt cgtaggcttg gtaggtcagc agccggaaat gcgaccgatg aatccaccaa gtggttttct ccggcggacg ccgacccgca ggtttcgcga ccgccgcgat gctggtcgcg tattggccga cgaacttgcg tcctatcgcg gcagggaccg gttgctcgtc ctcggccttg cccgcggtgg cgtccccgtc ggctgggaag tcgcgtcggc gctaggcgcc gaattggatg tatttctggt tcgcaagctc ggcgtgccgc agtggcgcga gctggcgatg ggcgcgttgg ccagtggggg cggggtcgtg atgaacgacg acgtggtttc cagcttgcgc atcaccgacc agcaggtgcg tgcggcgatc gacagcgaga cggcagagct gcagcggcgc gagctggcgt atcgcggcgg acgccctgtc gtcgatccgc gcgccaggat cgtgatcctg gttgacgacg gcatcgccac cggcgcgagc atgctggcgg cggtgcgcac catccgtgcc accggaccgg agtcgatcgt cgtcgcggtc ccggtcggtc cggccacagc ctgccgcgag ctcgcggcgg aagccgacga cgtggtgtgc gcaaccatgc cggcagcgtt tgaggccgtc ggccaggtct ataacgactt tcatcaggtc accgacgacg aggtccgcga gctgctcgcg acgccaacca caggcgcagc gacctaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa