MytuD.17396.a

POSSIBLE ALTERNATIVE RNA POLYMERASE SIGMA FACTOR SIGI

CENTER ID: MytuD.17396.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.17396.a.A1.GE30978 Full length( MytuD.17396.a ) 1 290
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.1335
RefSeq: NP_215705.1
TubercuList: Rv1189
MTB Network Portal: Rv1189
UniProt: P9WGH3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSQHDPVSAA WRAHRAYLVD LAFRMVGDIG VAEDMVQEAF SRLLRAPVGD IDDERGWLIV VTSRLCLDHI KSASTRRERP QDIAAWHDGD ASVSSVDPAD RVTLDDEVRL ALLIMLERLG PAERVVFVLH EIFGLPYQQI ATTIGSQAST CRQLAHRARR KINESRIAAS VEPAQHRVVT RAFIEACSNG DLDTLLEVLD PGVAGEIDAR KGVVVVGADR VGPTILRHWS HPATVLVAQP VCGQPAVLAF VNRALAGVLA LSIEAGKITK IHVLVQPSTL DPLRAELGGG
NT Sequence
ATGTCGCAAC ACGACCCGGT AAGTGCGGCC TGGCGGGCGC ATCGGGCCTA CCTGGTGGAC CTCGCGTTTC GTATGGTAGG TGACATCGGC GTGGCCGAAG ACATGGTGCA AGAGGCATTT TCCCGCTTGC TGCGGGCTCC GGTCGGCGAC ATCGACGACG AGCGTGGCTG GCTGATCGTG GTCACCAGCC GGCTGTGCCT GGATCACATC AAGTCGGCGT CGACACGCCG GGAGCGCCCG CAGGACATCG CCGCATGGCA CGACGGTGAC GCCAGCGTGT CATCGGTTGA CCCGGCTGAC CGGGTGACTC TCGACGACGA GGTCCGGCTG GCTTTGCTGA TCATGCTCGA GCGCCTCGGC CCCGCGGAGC GGGTGGTGTT CGTGCTGCAC GAGATCTTTG GGCTGCCCTA CCAGCAAATC GCCACGACGA TTGGCAGCCA GGCCTCCACA TGCCGGCAGC TGGCTCATCG GGCCCGTCGC AAGATCAACG AATCGCGCAT TGCGGCCAGC GTGGAGCCAG CCCAGCATCG CGTCGTCACC AGAGCTTTCA TCGAAGCCTG CTCCAACGGA GACCTGGACA CCCTGCTCGA GGTGCTGGAT CCGGGTGTCG CCGGCGAGAT CGACGCCCGC AAAGGCGTTG TCGTCGTGGG CGCGGATCGG GTTGGCCCGA CCATCCTGCG CCACTGGAGT CACCCCGCCA CCGTCCTGGT AGCCCAGCCG GTGTGCGGTC AACCGGCGGT GCTGGCCTTT GTCAACCGAG CGCTTGCCGG CGTGTTGGCC CTGTCGATCG AGGCCGGCAA GATCACAAAA ATCCATGTCT TAGTGCAGCC TTCAACATTG GACCCGTTAC GGGCCGAACT CGGCGGCGGT
Details for MytuD.17396.a.A1.GE30978
HARVESTED ON: 12/14/2010
SEQUENCED ON: 8/27/2015
EXPECTED MW: 33kDa
OBSERVED MW: 33kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSQHDPVSA AWRAHRAYLV DLAFRMVGDI GVAEDMVQEA FSRLLRAPVG DIDDERGWLI VVTSRLCLDH IKSASTRRER PQDIAAWHDG DASVSSVDPA DRVTLDDEVR LALLIMLERL GPAERVVFVL HEIFGLPYQQ IATTIGSQAS TCRQLAHRAR RKINESRIAA SVEPAQHRVV TRAFIEACSN GDLDTLLEVL DPGVAGEIDA RKGVVVVGAD RVGPTILRHW SHPATVLVAQ PVCGQPAVLA FVNRALAGVL ALSIEAGKIT KIHVLVQPST LDPLRAELGG G
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgtcgc aacacgaccc ggtaagtgcg gcctggcggg cgcatcgggc ctacctggtg gacctcgcgt ttcgtatggt aggtgacatc ggcgtggccg aagacatggt gcaagaggca ttttcccgct tgctgcgggc tccggtcggc gacatcgacg acgagcgtgg ctggctgatc gtggtcacca gccggctgtg cctggatcac atcaagtcgg cgtcgacacg ccgggagcgc ccgcaggaca tcgccgcatg gcacgacggt gacgccagcg tgtcatcggt tgacccggct gaccgggtga ctctcgacga cgaggtccgg ctggctttgc tgatcatgct cgagcgcctc ggccccgcgg agcgggtggt gttcgtgctg cacgagatct ttgggctgcc ctaccagcaa atcgccacga cgattggcag ccaggcctcc acatgccggc agctggctca tcgggcccgt cgcaagatca acgaatcgcg cattgcggcc agcgtggagc cagcccagca tcgcgtcgtc accagagctt tcatcgaagc ctgctccaac ggagacctgg acaccctgct cgaggtgctg gatccgggtg tcgccggcga gatcgacgcc cgcaaaggcg ttgtcgtcgt gggcgcggat cgggttggcc cgaccatcct gcgccactgg agtcaccccg ccaccgtcct ggtagcccag ccggtgtgcg gtcaaccggc ggtgctggcc tttgtcaacc gagcgcttgc cggcgtgttg gccctgtcga tcgaggccgg caagatcaca aaaatccatg tcttagtgca gccttcaaca ttggacccgt tacgggccga actcggcggc ggttaataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSQHDPVSA AWRAHRAYLV DLAFRMVGDI GVAEDMVQEA FSRLLRAPVG DIDDERGWLI VVTSRLCLDH IKSASTRRER PQDIAAWHDG DASVSSVDPA DRVTLDDEVR LALLIMLERL GPAERVVFVL HEIFGLPYQQ IATTIGSQAS TCRQLAHRAR RKINESRIAA SVEPAQHRVV TRAFIEACSN GDLDTLLEVL DPGVAGEIDA RKGVVVVGAD RVGPTILRHW SHPATVLVAQ PVCGQPAVLA FVNRALAGVL ALSIEAGKIT KIHVLVQPST LDPLRAELGG G
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcgcaacac gacccggtaa gtgcggcctg gcgggcgcat cgggcctacc tggtggacct cgcgtttcgt atggtaggtg acatcggcgt ggccgaagac atggtgcaag aggcattttc ccgcttgctg cgggctccgg tcggcgacat cgacgacgag cgtggctggc tgatcgtggt caccagccgg ctgtgcctgg atcacatcaa gtcggcgtcg acacgccggg agcgcccgca ggacatcgcc gcatggcacg acggtgacgc cagcgtgtca tcggttgacc cggctgaccg ggtgactctc gacgacgagg tccggctggc tttgctgatc atgctcgagc gcctcggccc cgcggagcgg gtggtgttcg tgctgcacga gatctttggg ctgccctacc agcaaatcgc cacgacgatt ggcagccagg cctccacatg ccggcagctg gctcatcggg cccgtcgcaa gatcaacgaa tcgcgcattg cggccagcgt ggagccagcc cagcatcgcg tcgtcaccag agctttcatc gaagcctgct ccaacggaga cctggacacc ctgctcgagg tgctggatcc gggtgtcgcc ggcgagatcg acgcccgcaa aggcgttgtc gtcgtgggcg cggatcgggt tggcccgacc atcctgcgcc actggagtca ccccgccacc gtcctggtag cccagccggt gtgcggtcaa ccggcggtgc tggcctttgt caaccgagcg cttgccggcg tgttggccct gtcgatcgag gccggcaaga tcacaaaaat ccatgtctta gtgcagcctt caacattgga cccgttacgg gccgaactcg gcggcggtta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a