SSGCID: Target Page

MytuD.17394.a

Putative uncharacterized protein

CENTER ID:	MytuD.17394.a
ORGANISM:	Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE:	Tuberculosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MytuD.17394.a.A1.GE30733	Full length( MytuD.17394.a )		1	246

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|83332.12.peg.3378
RefSeq:	NP_217543.1
TubercuList:	Rv3027c
MTB Network Portal:	Rv3027c
UniProt:	I6YEZ8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MVEAAQRLRY DVFSTTPGFA LPAAADTRRD GDRFDEYCDH LLVRDDDTGE LVGCYRMLAP AGAIAAGGLY TATEFDVCAF DPLRPSLVEM GRAVVREGHR NGGVVLLMWA GILAYLDRYG YDYVTGCVSV PIGGDGETPG SRLRGVRDFI LNRHAAPPQC QVYPYRPVRV DGRSLDDILP PPRPAVPPLM RGYLRLGARA CGEPAHDPDF GVGDFCLLLD KDHADTRYLR RLRSVAAASE MVNDAR

NT Sequence

ATGGTCGAGG CGGCGCAGCG GCTCCGCTAT GACGTGTTCA GCACTACGCC AGGCTTCGCA CTGCCGGCGG CCGCGGATAC GCGCCGGGAT GGCGACCGGT TCGACGAGTA CTGCGATCAC CTGCTGGTCC GCGACGACGA CACCGGTGAG CTGGTGGGTT GCTACCGGAT GCTGGCACCG GCGGGTGCCA TCGCCGCCGG AGGACTCTAC ACCGCAACGG AATTCGACGT TTGCGCGTTC GATCCGCTGC GGCCGTCGTT GGTGGAGATG GGCCGCGCCG TGGTGCGCGA GGGTCACCGC AACGGTGGGG TGGTGTTGTT GATGTGGGCG GGCATCCTGG CCTACCTGGA CCGATACGGC TATGACTATG TGACCGGATG TGTGTCAGTA CCAATCGGGG GAGACGGTGA AACGCCGGGC AGCCGGCTGC GTGGGGTGCG CGACTTCATC CTCAACCGGC ATGCCGCGCC GCCGCAGTGC CAGGTGTACC CCTACCGGCC GGTGCGCGTG GACGGGAGGT CCCTCGACGA CATTCTCCCT CCGCCACGGC CAGCAGTTCC CCCGCTGATG CGCGGTTACC TGAGACTGGG CGCGCGGGCC TGCGGGGAGC CGGCGCACGA CCCGGACTTT GGCGTGGGCG ACTTCTGCCT GCTACTGGAT AAGGACCACG CCGATACGCG ATATCTGCGA CGACTGCGGT CGGTTGCGGC GGCCTCGGAA ATGGTGAACG ACGCGCGA

Details for MytuD.17394.a.A1.GE30733

HARVESTED ON:	11/9/2010
SEQUENCED ON:	11/15/2010
EXPECTED MW:	29kDa
OBSERVED MW:	29kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMVEAAQRLR YDVFSTTPGF ALPAAADTRR DGDRFDEYCD HLLVRDDDTG ELVGCYRMLA PAGAIAAGGL YTATEFDVCA FDPLRPSLVE MGRAVVREGH RNGGVVLLMW AGILAYLDRY GYDYVTGCVS VPIGGDGETP GSRLRGVRDF ILNRHAAPPQ CQVYPYRPVR VDGRSLDDIL PPPRPAVPPL MRGYLRLGAR ACGEPAHDPD FGVGDFCLLL DKDHADTRYL RRLRSVAAAS EMVNDAR

Validated NT Sequence

attttgtttn ctttaagaag gaganatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtcg aggcggcgca gcggctccgc tatgacgtgt tcagcactac gccaggcttc gcactgccgg cggccgcgga tacgcgccgg gatggcgacc ggttcgacga gtactgcgat cacctgctgg tccgcgacga cgacaccggt gagctggtgg gttgctaccg gatgctggca ccggcgggtg ccatcgccgc cggaggactc tacaccgcaa cggaattcga cgtttgcgcg ttcgatccgc tgcggccgtc gttggtggag atgggccgcg ccgtggtgcg cgagggtcac cgcaacggtg gggtggtgtt gttgatgtgg gcgggcatcc tggcctacct ggaccgatac ggctatgact atgtgaccgg atgtgtgtca gtaccaatcg ggggagacgg tgaaacgccg ggcagccggc tgcgtggggt gcgcgacttc atcctcaacc ggcatgccgc gccgccgcag tgccaggtgt acccctaccg gccggtgcgc gtggacggga ggtccctcga cgacattctc cctccgccac ggccagcagt tcccccgctg atgcgcggtt acctgagact gggcgcgcgg gcctgcgggg agccggcgca cgacccggac tttggcgtgg gcgacttctg cctgctactg gataaggacc acgccgatac gcgatatctg cgacgactgc ggtcggttgc ggcggcctcg gaaatggtga acgacgcgcg ataataaaca gcacgaacaa gttctgcagc cnagcttctc gaggnnccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctnnn nnnna

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtcgaggcg gcgcagcggc tccgctatga cgtgttcagc actacgccag gcttcgcact gccggcggcc gcggatacgc gccgggatgg cgaccggttc gacgagtact gcgatcacct gctggtccgc gacgacgaca ccggtgagct ggtgggttgc taccggatgc tggcaccggc gggtgccatc gccgccggag gactctacac cgcaacggaa ttcgacgttt gcgcgttcga tccgctgcgg ccgtcgttgg tggagatggg ccgcgccgtg gtgcgcgagg gtcaccgcaa cggtggggtg gtgttgttga tgtgggcggg catcctggcc tacctggacc gatacggcta tgactatgtg accggatgtg tgtcagtacc aatcggggga gacggtgaaa cgccgggcag ccggctgcgt ggggtgcgcg acttcatcct caaccggcat gccgcgccgc cgcagtgcca ggtgtacccc taccggccgg tgcgcgtgga cgggaggtcc ctcgacgaca ttctccctcc gccacggcca gcagttcccc cgctgatgcg cggttacctg agactgggcg cgcgggcctg cggggagccg gcgcacgacc cggactttgg cgtgggcgac ttctgcctgc tactggataa ggaccacgcc gatacgcgat atctgcgacg actgcggtcg gttgcggcgg cctcggaaat ggtgaacgac gcgcgataaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa