SSGCID: Target Page

MytuD.17393.a

PROBABLE PHOH-LIKE PROTEIN PHOH2 (PHOSPHATE STARVATION-INDUCIBLE PROTEIN PSIH)

CENTER ID:	MytuD.17393.a
ORGANISM:	Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE:	Tuberculosis
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MytuD.17393.a.A1.GE30732	Full length( MytuD.17393.a )		1	433

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MytuD.17393.a.A1.PS01021	Full length( MytuD.17393.a )		1	433

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|83332.12.peg.1226
RefSeq:	NP_215611.1
TubercuList:	Rv1095
MTB Network Portal:	Rv1095
UniProt:	O53443

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTDTRTYVLD TSVLLSDPWA CSRFAEHDVV VPLVVISELE AKRHHHELGW FARQALRLFD DLRLEHGRLD QPIPVGTQGG TLHVELNHTD PAVLPAGFRT DSNDSRILSC AANLAAEGKR VTLVSKDIPL RVKAAAVGLA ADEYHAQDVV VSGWSGMHEL ETASADIDAL FADGEIDLVE ARDLPCHTGI RLLGGGSHAL GRVNAHKRVQ LVRGDREAFG LRGRSAEQRV ALDLLLDESV GIVSLGGKAG TGKSALALCA GLEAVLERRT HRKVVVFRPL YAVGGQELGY LPGSESEKMG PWAQAVFDTL EGLASPAVLE EVLSRGMLEV LPLTHIRGRS LHDSFVIVDE AQSLERNVLL TVLSRLGTGS RVVLTHDIAQ RDNLRVGRHD GVAAVIEKLK GHPLFAHITL LRSERSPIAA LVTEMLEEIT GPR

NT Sequence

GTGACCGATA CCCGCACGTA CGTGCTCGAC ACCTCTGTGC TGCTGTCCGA TCCGTGGGCG TGCAGCCGGT TCGCCGAACA CGATGTGGTG GTTCCGTTGG TGGTGATCAG CGAGCTAGAA GCCAAGCGCC ACCACCACGA GCTGGGATGG TTCGCCCGCC AGGCGTTGCG TCTGTTCGAC GATCTGCGCC TAGAACACGG GCGGTTGGAT CAGCCGATTC CGGTTGGCAC CCAAGGCGGT ACGCTGCACG TCGAACTCAA TCACACCGAC CCGGCGGTGC TGCCCGCAGG CTTTCGCACC GACAGCAACG ACTCGAGGAT CTTGAGTTGC GCCGCCAACC TCGCCGCCGA GGGCAAGCGG GTCACGTTGG TCAGCAAGGA CATTCCGCTG CGCGTTAAGG CCGCCGCGGT GGGGCTGGCC GCCGACGAGT ACCACGCGCA GGACGTCGTT GTGTCCGGAT GGTCGGGGAT GCACGAGCTC GAGACCGCTT CCGCGGATAT CGATGCGTTG TTCGCCGATG GCGAGATCGA CCTGGTCGAA GCCCGGGACC TACCGTGTCA CACCGGGATT CGGTTGCTGG GCGGCGGTTC CCACGCGCTG GGCCGGGTCA ATGCGCATAA ACGTGTTCAG CTGGTGCGAG GTGACCGTGA GGCGTTCGGT CTGCGTGGCC GCTCCGCCGA GCAGCGGGTG GCGCTGGATT TGCTGCTCGA TGAGTCGGTG GGCATCGTGT CGCTGGGCGG CAAAGCCGGC ACGGGCAAGT CCGCTTTGGC GTTGTGTGCG GGTCTGGAAG CCGTGCTGGA GCGACGCACC CACCGCAAGG TGGTGGTCTT CCGCCCGCTG TACGCGGTCG GCGGCCAGGA GCTGGGCTAC CTGCCCGGTA GCGAGAGCGA GAAGATGGGC CCGTGGGCGC AGGCGGTCTT CGACACCCTC GAGGGGCTGG CCAGCCCGGC GGTGCTCGAG GAAGTGCTGT CCCGTGGCAT GCTCGAGGTG CTGCCGCTGA CCCACATCCG GGGCCGCTCG TTGCATGACT CGTTCGTCAT CGTCGACGAG GCACAGTCGC TGGAGCGCAA TGTGTTGCTG ACCGTGCTGT CCCGGTTGGG GACCGGTTCC CGGGTGGTGT TGACCCACGA CATCGCCCAG CGCGACAACC TGCGGGTCGG CCGCCACGAC GGGGTCGCCG CGGTGATCGA GAAGCTCAAA GGTCATCCGT TGTTCGCCCA CATCACCTTG CTGCGCAGTG AGCGCTCGCC GATCGCCGCG CTGGTCACCG AGATGCTCGA GGAGATCACC GGGCCGCGC

Details for MytuD.17393.a.A1.GE30732

HARVESTED ON:	11/9/2010
SEQUENCED ON:	11/15/2010
EXPECTED MW:	49kDa
OBSERVED MW:	50kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	60

Validated AA Sequence

MHELETASAD IDALFADGEI DLVEARDLPC HTGIRLLGGG SHALGRVNAH KRVQLVRGDR EAFGLRGRSA EQRVALDLLL DESVGIVSLG GKAGTGKSAL ALCAGLEAVL ERRTHRKVVV FRPLYAVGGQ ELGYLPGSES EKMGPWAQAV FDTLEGLASP AVLEEVLSRG MLEVLPLTHI RGRSLHDSFV IVDEAQSLER NVLLTVLSRL GTGSRVVLTH DIAQRDNLRV GRHDGVAAVI EKLKGHPLFA HITLLRSERS PIAALVTEML EEITGP

Validated NT Sequence

gcgcggcccg gtgatctcct cgagcatctc ggtgaccagc gcggcgatcg gcgagcgctc actgcgcagc aaggtgatgt gggcgaacaa cggatgacct ttgagcttct cgatcaccgc ggcgaccccg tcgtggcggc cgacccgcag gttgtcgcgc tgggcgatgt cgtgggtcaa caccacccgg gaaccggtcc ccaaccggga cagcacggtc agcaacacat tgcgctccag cgactgtgcc tcgtcgacga tgacgaacga gtcatgcaac gagcggcccc ggatgtgggt cagcggcagc acctcgagca tgccacggga cagcacttcc tcgagcaccg ccgggctggc cagcccctcg agggtgtcga agaccgcctg cgcccacggg cccatcttct cgctctcgct accgggcagg tagcccagct cctggccgcc gaccgcgtac agcgggcgga agaccaccac cttgcggtgg gtgcgtcgct ccagcacggc ttccagaccc gcacacaacg ccaaagcgga cttgcccgtg ccggctttgc cgcccagcga cacgatgccc accgactcat cgagcagcaa atccagcgcc acccgctgct cggcggagcg gccacgcaga ccgaacgcct cacggtcacc tcgcaccagc tgaacacgtt tatgcgcatt gacccggccc agcgcgtggg aaccgccgcc cagcaaccga atcccggtgt gacacggtag gtcccgggct tcgaccaggt cgatctcgcc atcggcgaac aacgcatcga tatccgcgga agcggtctcg agctcgtgca tccccgacca tccggacaca acgacgtcct gcgcgtggta ctcgtcggcg gccagcccca ccgcggcggc cttaacgcgc agcggaatgt ccttgctgac caacgtgacc cgcttgccct cggcggcgag gttggcggcg caactcaaga tcctcgagtc gttgctgtcg gtgcgaaagc ctgcgggcag caccgccggg tcggtgtgat tgagttcgac gtgcagcgta ccgccttggg tgccaaccgg aatcggctga tccaaccgcc cgtgttctag gcgcagatcg tcgaacagac gcaacgcctg gcgggcgaac catcccagct cgtggtggtg gcgcttggct tctagctcgc tgatcaccac caacggaacc accacatcgt gttcggcgaa ccggctgcac gcccacggat cggacagcag cacagagntg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVTDTRTYV LDTSVLLSDP WACSRFAEHD VVVPLVVISE LEAKRHHHEL GWFARQALRL FDDLRLEHGR LDQPIPVGTQ GGTLHVELNH TDPAVLPAGF RTDSNDSRIL SCAANLAAEG KRVTLVSKDI PLRVKAAAVG LAADEYHAQD VVVSGWSGMH ELETASADID ALFADGEIDL VEARDLPCHT GIRLLGGGSH ALGRVNAHKR VQLVRGDREA FGLRGRSAEQ RVALDLLLDE SVGIVSLGGK AGTGKSALAL CAGLEAVLER RTHRKVVVFR PLYAVGGQEL GYLPGSESEK MGPWAQAVFD TLEGLASPAV LEEVLSRGML EVLPLTHIRG RSLHDSFVIV DEAQSLERNV LLTVLSRLGT GSRVVLTHDI AQRDNLRVGR HDGVAAVIEK LKGHPLFAHI TLLRSERSPI AALVTEMLEE ITGPR

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgaccgat acccgcacgt acgtgctcga cacctctgtg ctgctgtccg atccgtgggc gtgcagccgg ttcgccgaac acgatgtggt ggttccgttg gtggtgatca gcgagctaga agccaagcgc caccaccacg agctgggatg gttcgcccgc caggcgttgc gtctgttcga cgatctgcgc ctagaacacg ggcggttgga tcagccgatt ccggttggca cccaaggcgg tacgctgcac gtcgaactca atcacaccga cccggcggtg ctgcccgcag gctttcgcac cgacagcaac gactcgagga tcttgagttg cgccgccaac ctcgccgccg agggcaagcg ggtcacgttg gtcagcaagg acattccgct gcgcgttaag gccgccgcgg tggggctggc cgccgacgag taccacgcgc aggacgtcgt tgtgtccgga tggtcgggga tgcacgagct cgagaccgct tccgcggata tcgatgcgtt gttcgccgat ggcgagatcg acctggtcga agcccgggac ctaccgtgtc acaccgggat tcggttgctg ggcggcggtt cccacgcgct gggccgggtc aatgcgcata aacgtgttca gctggtgcga ggtgaccgtg aggcgttcgg tctgcgtggc cgctccgccg agcagcgggt ggcgctggat ttgctgctcg atgagtcggt gggcatcgtg tcgctgggcg gcaaagccgg cacgggcaag tccgctttgg cgttgtgtgc gggtctggaa gccgtgctgg agcgacgcac ccaccgcaag gtggtggtct tccgcccgct gtacgcggtc ggcggccagg agctgggcta cctgcccggt agcgagagcg agaagatggg cccgtgggcg caggcggtct tcgacaccct cgaggggctg gccagcccgg cggtgctcga ggaagtgctg tcccgtggca tgctcgaggt gctgccgctg acccacatcc ggggccgctc gttgcatgac tcgttcgtca tcgtcgacga ggcacagtcg ctggagcgca atgtgttgct gaccgtgctg tcccggttgg ggaccggttc ccgggtggtg ttgacccacg acatcgccca gcgcgacaac ctgcgggtcg gccgccacga cggggtcgcc gcggtgatcg agaagctcaa aggtcatccg ttgttcgccc acatcacctt gctgcgcagt gagcgctcgc cgatcgccgc gctggtcacc gagatgctcg aggagatcac cgggccgcgc taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa

Details for MytuD.17393.a.A1.PS01021

PURIFICATION DATe:	3/30/2011
CONCENTRATION:	36.2mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	2
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	60

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGACCGAT ACCCGCACGT ACGTGCTCGA CACCTCTGTG CTGCTGTCCG ATCCGTGGGC GTGCAGCCGG TTCGCCGAAC ACGATGTGGT GGTTCCGTTG GTGGTGATCA GCGAGCTAGA AGCCAAGCGC CACCACCACG AGCTGGGATG GTTCGCCCGC CAGGCGTTGC GTCTGTTCGA CGATCTGCGC CTAGAACACG GGCGGTTGGA TCAGCCGATT CCGGTTGGCA CCCAAGGCGG TACGCTGCAC GTCGAACTCA ATCACACCGA CCCGGCGGTG CTGCCCGCAG GCTTTCGCAC CGACAGCAAC GACTCGAGGA TCTTGAGTTG CGCCGCCAAC CTCGCCGCCG AGGGCAAGCG GGTCACGTTG GTCAGCAAGG ACATTCCGCT GCGCGTTAAG GCCGCCGCGG TGGGGCTGGC CGCCGACGAG TACCACGCGC AGGACGTCGT TGTGTCCGGA TGGTCGGGGA TGCACGAGCT CGAGACCGCT TCCGCGGATA TCGATGCGTT GTTCGCCGAT GGCGAGATCG ACCTGGTCGA AGCCCGGGAC CTACCGTGTC ACACCGGGAT TCGGTTGCTG GGCGGCGGTT CCCACGCGCT GGGCCGGGTC AATGCGCATA AACGTGTTCA GCTGGTGCGA GGTGACCGTG AGGCGTTCGG TCTGCGTGGC CGCTCCGCCG AGCAGCGGGT GGCGCTGGAT TTGCTGCTCG ATGAGTCGGT GGGCATCGTG TCGCTGGGCG GCAAAGCCGG CACGGGCAAG TCCGCTTTGG CGTTGTGTGC GGGTCTGGAA GCCGTGCTGG AGCGACGCAC CCACCGCAAG GTGGTGGTCT TCCGCCCGCT GTACGCGGTC GGCGGCCAGG AGCTGGGCTA CCTGCCCGGT AGCGAGAGCG AGAAGATGGG CCCGTGGGCG CAGGCGGTCT TCGACACCCT CGAGGGGCTG GCCAGCCCGG CGGTGCTCGA GGAAGTGCTG TCCCGTGGCA TGCTCGAGGT GCTGCCGCTG ACCCACATCC GGGGCCGCTC GTTGCATGAC TCGTTCGTCA TCGTCGACGA GGCACAGTCG CTGGAGCGCA ATGTGTTGCT GACCGTGCTG TCCCGGTTGG GGACCGGTTC CCGGGTGGTG TTGACCCACG ACATCGCCCA GCGCGACAAC CTGCGGGTCG GCCGCCACGA CGGGGTCGCC GCGGTGATCG AGAAGCTCAA AGGTCATCCG TTGTTCGCCC ACATCACCTT GCTGCGCAGT GAGCGCTCGC CGATCGCCGC GCTGGTCACC GAGATGCTCG AGGAGATCAC CGGGCCGCGC TAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA