MytuD.17389.a
Uncharacterized protein Rv3404c/MT3512
| CENTER ID: | MytuD.17389.a |
| ORGANISM: | Mycobacterium tuberculosis H37Rv |
| ASSOCIATED DISEASE: | Tuberculosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MytuD.17389.a.A1.GE30729 | Full length( MytuD.17389.a ) | 1 | 234 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
4PZU
DEPOSITED: 3/31/2014
DETERMINATION: XRay
CLONE: MytuD.17389.a.A1.GE30729
PROTEIN: MytuD.17389.a.A1.PW30616
4Q12
DEPOSITED: 4/2/2014
DETERMINATION: XRay
CLONE: MytuD.17389.a.A1.GE30729
PROTEIN: MytuD.17389.a.A1.PW30616
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_193480 |
| PATRIC ID: | fig|83332.12.peg.3801 |
| RefSeq: | NP_217921.1 |
| TubercuList: | Rv3404c |
| MTB Network Portal: | Rv3404c |
| UniProt: | P9WKZ3 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTILILTDNV HAHALAVDLQ ARHGDMDVYQ SPIGQLPGVP RCDVAERVAE IVERYDLVLS FHCKQRFPAA LIDGVRCVNV HPGFNPYNRG WFPQVFSIID GQKVGVTIHE IDDQLDHGPI IAQRECAIES WDSSGSVYAR LMDIERELVL EHFDAIRDGS YTAKSPATEG NLNLKKDFEQ LRRLDLNERG TFGHFLNRLR ALTHDDFRNA WFVDASGRKV FVRVVLEPEK PAEA
NT Sequence
GTGACGATAT TGATCCTGAC CGACAACGTC CACGCCCATG CTCTGGCGGT CGATCTGCAG GCCAGGCATG GCGATATGGA CGTCTATCAG TCCCCCATCG GCCAGCTGCC GGGTGTCCCG CGATGTGATG TCGCAGAGCG CGTCGCGGAA ATCGTGGAGC GGTATGACCT CGTCCTTTCC TTCCACTGTA AACAGAGGTT TCCCGCCGCT TTGATCGATG GGGTCAGGTG TGTGAATGTT CATCCGGGTT TCAACCCCTA CAACCGCGGC TGGTTTCCCC AGGTCTTCTC GATCATCGAC GGGCAAAAAG TCGGCGTGAC GATCCACGAG ATCGACGATC AGTTGGACCA TGGTCCGATC ATCGCCCAGC GGGAATGCGC GATCGAGTCG TGGGATTCCT CGGGAAGTGT CTACGCCCGG CTGATGGACA TCGAGCGTGA GTTGGTGCTG GAACATTTCG ACGCCATCCG GGACGGCAGC TACACGGCTA AATCGCCGGC CACCGAGGGC AACCTCAACC TGAAAAAGGA TTTCGAACAA CTCCGGCGGC TAGACCTGAA CGAGCGCGGA ACGTTTGGGC ATTTCCTGAA TCGCCTGCGC GCGTTGACCC ATGATGATTT CCGCAACGCT TGGTTCGTCG ATGCGTCAGG CCGCAAGGTG TTTGTCCGCG TCGTGCTCGA ACCGGAGAAG CCCGCGGAAG CC
Details for MytuD.17389.a.A1.GE30729
| HARVESTED ON: | 11/9/2010 |
| SEQUENCED ON: | 11/15/2010 |
| EXPECTED MW: | 28kDa |
| OBSERVED MW: | 29kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 99 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVTILILTD NVHAHALAVD LQARHGDMDV YQSPIGQLPG VPRCDVAERV AEIVERYDLV LSFHCKQRFP AALIDGVRCV NVHPGFNPYN RGWFPQVFSI IDGQKVGVTI HEIDDQLDHG PIIAQRECAI ESWDSSGSVY ARLMDIEREL VLEHFDAIRD GSYTAKSPAT EGNLNLKKDF EQLRRLDLNE RGTFGHFLNR LRALTHDDFR NAWFVDASGR KVFVRVVLEP EKPA
Validated NT Sequence
ccgcgggctt ctccggttcg agcacgacgc ggacaaacac cttgcggcct gacgcatcga cgaaccaagc gttgcggaaa tcatcatggg tcaacgcgcg caggcgattc aggaaatgcc caaacgttcc gcgctcgttc aggtctagcc gccggagttg ttcgaaatcc tttttcaggt tgaggttgcc ctcggtggcc ggcgatttag ccgtgtagct gccgtcccgg atggcgtcga aatgttccag caccaactca cgctcgatgt ccatcagccg ggcgtagaca cttcccgagg aatcccacga ctcgatcgcg cattcccgct gggcgatgat cggaccatgg tccaactgat cgtcgatctc gtggatcgtc acgccgactt tttgcccgtc gatgatcgag aagacctggg gaaaccagcc gcggttgtag gggttgaaac ccggatgaac attcacacac ctgaccccat cgatcaaagc ggcgggaaac ctctgtttac agtggaagga aaggacgagg tcataccgct ccacgatttc cgcgacgcgc tctgcgacat cacatcgcgg gacacccggc agctggccga tgggggactg atagacgtcc atatcgccat gcctggcctg cagatcgacc gccagagcat gggcgtggac gttgtcggtc aggatcaata tcgtcaccat cgaaccagga ccctgggtct gagcttccag ggtacccata tgatggtgat ggtgatgagc cat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVTILILTD NVHAHALAVD LQARHGDMDV YQSPIGQLPG VPRCDVAERV AEIVERYDLV LSFHCKQRFP AALIDGVRCV NVHPGFNPYN RGWFPQVFSI IDGQKVGVTI HEIDDQLDHG PIIAQRECAI ESWDSSGSVY ARLMDIEREL VLEHFDAIRD GSYTAKSPAT EGNLNLKKDF EQLRRLDLNE RGTFGHFLNR LRALTHDDFR NAWFVDASGR KVFVRVVLEP EKPAEA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgacgata ttgatcctga ccgacaacgt ccacgcccat gctctggcgg tcgatctgca ggccaggcat ggcgatatgg acgtctatca gtcccccatc ggccagctgc cgggtgtccc gcgatgtgat gtcgcagagc gcgtcgcgga aatcgtggag cggtatgacc tcgtcctttc cttccactgt aaacagaggt ttcccgccgc tttgatcgat ggggtcaggt gtgtgaatgt tcatccgggt ttcaacccct acaaccgcgg ctggtttccc caggtcttct cgatcatcga cgggcaaaaa gtcggcgtga cgatccacga gatcgacgat cagttggacc atggtccgat catcgcccag cgggaatgcg cgatcgagtc gtgggattcc tcgggaagtg tctacgcccg gctgatggac atcgagcgtg agttggtgct ggaacatttc gacgccatcc gggacggcag ctacacggct aaatcgccgg ccaccgaggg caacctcaac ctgaaaaagg atttcgaaca actccggcgg ctagacctga acgagcgcgg aacgtttggg catttcctga atcgcctgcg cgcgttgacc catgatgatt tccgcaacgc ttggttcgtc gatgcgtcag gccgcaaggt gtttgtccgc gtcgtgctcg aaccggagaa gcccgcggaa gcctaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa