MytuD.17369.b

Transcription termination factor Rho (Rv1297)

CENTER ID: MytuD.17369.b
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:
IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
MytuD.17369.b.MB2.GE40924 truncation 1 226 602
MytuD.17369.b.MB3.GE40925 truncation 2 257 602
MytuD.17369.b.MB4.GE40926 truncation 3 271 602
MytuD.17369.b.MB5.GE40927 truncation 4 291 602
MytuD.17369.b.MB6.GE40928 truncation 5 300 602
MytuD.17369.b.MB7.GE40929 truncation 6 312 602
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.1453
RefSeq: WP_003898814.1
TubercuList: Rv1297
MTB Network Portal: Rv1297
UniProt: P9WHF3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTDTDLITAG ESTDGKPSDA AATDPPDLNA DEPAGSLATM VLPELRALAN RAGVKGTSGM RKNELIAAIE EIRRQANGAP AVDRSAQEHD KGDRPPSSEA PATQGEQTPT EQIDSQSQQV RPERRSATRE AGPSGSGERA GTAADDTDNR QGGQQDAKTE ERGTDAGGDQ GGDQQASGGQ QARGDEDGEA RQGRRGRRFR DRRRRGERSG DGAEAELRED DVVQPVAGIL DVLDNYAFVR TSGYLPGPHD VYVSMNMVRK NGMRRGDAVT GAVRVPKEGE QPNQRQKFNP LVRLDSINGG SVEDAKKRPE FGKLTPLYPN QRLRLETSTE RLTTRVIDLI MPIGKGQRAL IVSPPKAGKT TILQDIANAI TRNNPECHLM VVLVDERPEE VTDMQRSVKG EVIASTFDRP PSDHTSVAEL AIERAKRLVE QGKDVVVLLD SITRLGRAYN NASPASGRIL SGGVDSTALY PPKRFLGAAR NIEEGGSLTI IATAMVETGS TGDTVIFEEF KGTGNAELKL DRKIAERRVF PAVDVNPSGT RKDELLLSPD EFAIVHKLRR VLSGLDSHQA IDLLMSQLRK TKNNYEFLVQ VSKTTPGSMD SD
NT Sequence
gtgaccgata cggacctcat tacggctggc gaaagtaccg acggcaagcc gtcggatgcc gctgccacag atcccccaga cctcaacgcc gacgagccgg ccggctcgct ggccaccatg gtgctgcccg aactgcgtgc gctggctaat cgagccggcg tgaagggaac atcgggtatg cggaagaacg aactgatcgc tgcgattgag gagatcaggc gacaggccaa cggcgcccca gccgttgacc ggtcggctca agagcacgac aagggcgacc ggccgcccag ttccgaggca ccggccaccc agggggaaca gaccccgacc gaacagatcg attcccaaag ccaacaggtc cgcccggagc ggcgcagcgc cacccgtgaa gcgggaccct ccggctccgg tgagcgtgcg ggcacagccg cagacgacac cgacaaccgc caaggcggtc aacaggacgc caagaccgag gagcgtggca ccgacgcggg tggcgaccaa gggggtgacc agcaggcttc gggcggtcag caggcgcgcg gcgacgagga cggagaagcg cgtcagggcc ggcgcggacg ccggttccgc gatcggcggc gccgcggtga acgatccggc gacggcgccg aggctgaact gcgtgaggac gacgtcgtcc agccggtagc cggcatactc gacgtcctgg acaactacgc gtttgtgcgc acctccggct acctacccgg tccgcacgac gtgtatgtgt cgatgaacat ggtgcgcaag aacggcatgc gccgtggtga tgcggtgacc ggtgcggtgc gggtgcccaa ggaaggggag caacccaacc agcggcagaa gttcaacccg ctggtccgcc tggacagcat caacggcgga tcggtcgaag acgccaagaa gcggcccgag ttcggcaaac tgacgccgtt gtaccccaac cagcggcttc gtctggaaac cagtaccgag cggctgacca cccgggtcat cgacctcatc atgccgatcg gcaagggtca acgcgcgttg attgtgtcgc cgcccaaagc gggcaagaca acgatcctgc aggacatcgc caacgcgatc accaggaaca acccggaatg ccacctcatg gtcgtgctcg tcgacgagcg gcctgaggag gtcaccgata tgcagcgctc ggtcaaaggc gaggtcatcg cttcaacttt cgaccggccg ccgtcggacc acacgtcggt cgccgagctg gcgatcgaac gcgccaagcg gctggtggag caaggcaagg acgtcgtggt gctgctcgat tcaatcaccc ggctaggccg cgcttacaac aacgcgtcgc cggcgtcggg ccggatcctg tccggtggtg tcgattccac ggcgttgtac ccgcccaagc gcttcctggg ggccgcgcgc aacatcgaag agggcgggtc gctgaccatc atcgccactg cgatggtcga gaccgggtcc actggtgaca cggtcatttt cgaggagttc aagggcaccg gcaacgccga gctcaagctg gaccgcaaga tcgccgagcg gcgggttttc cctgcggtcg acgtgaaccc ttctggaacc cgcaaggacg agctactgct gtcgcccgac gagttcgcta ttgtgcacaa gctgcgccgc gtgctatcgg gcctggattc ccaccaggcc atcgacctgc tgatgtcgca gctgcgtaag acgaagaaca actacgaatt ccttgttcag gtgtccaaga ccacgccagg gtccatggac agcgactga
Details for MytuD.17369.b.MB2.GE40924
HARVESTED ON: 9/23/2016
SEQUENCED ON: 9/9/2016
EXPECTED MW: 86kDa
OBSERVED MW: 90kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: Rosetta2 (DE3)
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 51
Validated AA Sequence
MNADTDYSIA EAAFNKGETA MTINGPWAWS NIDTSKVNYG VTVLPTFKGQ PSKPFVGVLS AGINAASPNK ELAKEFLENY LLTDEGLEAV NKDKPLGAVA LKSYEEELAK DPRIAATMEN AQKGEIMPNI PQMSAFWYAV RTAVINAASG RQTVDEALKD AQTNSSSNNN NNNNNNNLGI EGRISEGTLE AQTQGPGSRV AGILDVLDNY AFVRTSGYLP GPHDVYVSMN MVRKNGMRRG DAVTGAVRVP KEGEQPNQRQ KFNPLVRLDS INGGSVEDAK KRPEFGKLTP LYPNQRLRLE TSTERLTTRV IDLIMPIGKG QRALIVSPPK AGKTTILQDI ANAITRNNPE CHLMVVLVDE RPEEVTDMQR SVKGEVIAST FDRPPSDHTS VAELAIERAK RLVEQGKDV
Validated NT Sequence
ataacgctgg cgcgaaagcg ggtctgacct tcctggttga cctgattaaa aacaaacaca tgaatgcaga caccgattac tccatcgcag aagctgcctt taataaaggc gaaacagcga tgaccatcaa cggcccgtgg gcatggtcca acatcgacac cagcaaagtg aattatggtg taacggtact gccgaccttc aagggtcaac catccaaacc gttcgttggc gtgctgagcg caggtattaa cgccgccagt ccgaacaaag agctggcaaa agagttcctc gaaaactatc tgctgactga tgaaggtctg gaagcggtta ataaagacaa accgctgggt gccgtagcgc tgaagtctta cgaggaagag ttggcgaaag atccacgtat tgccgccact atggaaaacg cccagaaagg tgaaatcatg ccgaacatcc cgcagatgtc cgctttctgg tatgccgtgc gtactgcggt gatcaacgcc gccagcggtc gtcagactgt cgatgaagcc ctgaaagacg cgcagactaa ttcgagctcg aacaacaaca acaacaacaa taacaacaac ctcgggatcg agggaaggat ttcagaaggt accctggaag ctcagaccca gggtcctggt tcgcgagtgg ctggcattct ggatgttctg gacaactatg cctttgtgcg taccagtggt tacctgccgg gtccgcacga tgtttatgtc tccatgaaca tggttcgtaa aaatggtatg cgtcgcggcg atgctgtcac cggtgccgtg cgtgttccga aagaaggcga acagccgaac cagcgtcaaa aatttaatcc gctggtccgc ctggacagca tcaacggcgg ttctgtggaa gatgcgaaaa aacgtccgga attcggtaaa ctgaccccgc tgtacccgaa tcagcgtctg cgcctggaaa ccagtacgga acgtctgacc acgcgcgtta tcgatctgat tatgccgatc ggcaagggtc agcgtgccct gattgtgtcc ccgccgaaag caggcaaaac cacgattctg caagatatcg caaatgctat tacgcgcaac aatccggaat gccatctgat ggtcgtgctg gttgacgaac gtccggaaga agtcaccgat atgcagcgct cagttaaagg tgaagtcatc gcctcgacgt ttgaccgtcc gccgagtgat cacaccagcg tggcagaact ggctattgaa cgtgcgaaac gcctggttga acagggcaaa gacgttgt
Expected Protein Sequence
MAHHHHHHMK IEEGKLVIWI NGDKGYNGLA EVGKKFEKDT GIKVTVEHPD KLEEKFPQVA ATGDGPDIIF WAHDRFGGYA QSGLLAEITP DKAFQDKLYP FTWDAVRYNG KLIAYPIAVE ALSLIYNKDL LPNPPKTWEE IPALDKELKA KGKSALMFNL QEPYFTWPLI AADGGYAFKY ENGKYDIKDV GVDNAGAKAG LTFLVDLIKN KHMNADTDYS IAEAAFNKGE TAMTINGPWA WSNIDTSKVN YGVTVLPTFK GQPSKPFVGV LSAGINAASP NKELAKEFLE NYLLTDEGLE AVNKDKPLGA VALKSYEEEL AKDPRIAATM ENAQKGEIMP NIPQMSAFWY AVRTAVINAA SGRQTVDEAL KDAQTNSSSN NNNNNNNNNL GIEGRISEGT LEAQTQGPGS VAGILDVLDN YAFVRTSGYL PGPHDVYVSM NMVRKNGMRR GDAVTGAVRV PKEGEQPNQR QKFNPLVRLD SINGGSVEDA KKRPEFGKLT PLYPNQRLRL ETSTERLTTR VIDLIMPIGK GQRALIVSPP KAGKTTILQD IANAITRNNP ECHLMVVLVD ERPEEVTDMQ RSVKGEVIAS TFDRPPSDHT SVAELAIERA KRLVEQGKDV VVLLDSITRL GRAYNNASPA SGRILSGGVD STALYPPKRF LGAARNIEEG GSLTIIATAM VETGSTGDTV IFEEFKGTGN AELKLDRKIA ERRVFPAVDV NPSGTRKDEL LLSPDEFAIV HKLRRVLSGL DSHQAIDLLM SQLRKTKNNY EFLVQVSKTT PGSMDSD
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcaca tgaaaatcga agaaggtaaa ctggtaatct ggattaacgg cgataaaggc tataacggtc tcgctgaagt cggtaagaaa ttcgagaaag ataccggaat taaagtcacc gttgagcatc cggataaact ggaagagaaa ttcccacagg ttgcggcaac tggcgatggc cctgacatta tcttctgggc acacgaccgc tttggtggct acgctcaatc tggcctgttg gctgaaatca ccccggacaa agcgttccag gacaagctgt atccgtttac ctgggatgcc gtacgttaca acggcaagct gattgcttac ccgatcgctg ttgaagcgtt atcgctgatt tataacaaag atctgctgcc gaacccgcca aaaacctggg aagagatccc ggcgctggat aaagaactga aagcgaaagg taagagcgcg ctgatgttca acctgcaaga accgtacttc acctggccgc tgattgctgc tgacgggggt tatgcgttca agtatgaaaa cggcaagtac gacattaaag acgtgggcgt ggataacgct ggcgcgaaag cgggtctgac cttcctggtt gacctgatta aaaacaaaca catgaatgca gacaccgatt actccatcgc agaagctgcc tttaataaag gcgaaacagc gatgaccatc aacggcccgt gggcatggtc caacatcgac accagcaaag tgaattatgg tgtaacggta ctgccgacct tcaagggtca accatccaaa ccgttcgttg gcgtgctgag cgcaggtatt aacgccgcca gtccgaacaa agagctggca aaagagttcc tcgaaaacta tctgctgact gatgaaggtc tggaagcggt taataaagac aaaccgctgg gtgccgtagc gctgaagtct tacgaggaag agttggcgaa agatccacgt attgccgcca ctatggaaaa cgcccagaaa ggtgaaatca tgccgaacat cccgcagatg tccgctttct ggtatgccgt gcgtactgcg gtgatcaacg ccgccagcgg tcgtcagact gtcgatgaag ccctgaaaga cgcgcagact aattcgagct cgaacaacaa caacaataac aataacaaca acctcgggat cgagggaagg atttcagaag gtaccctgga agctcagacc cagggtcctg gttcggtagc cggcatactc gacgtcctgg acaactacgc gtttgtgcgc acctccggct acctacccgg tccgcacgac gtgtatgtgt cgatgaacat ggtgcgcaag aacggcatgc gccgtggtga tgcggtgacc ggtgcggtgc gggtgcccaa ggaaggggag caacccaacc agcggcagaa gttcaacccg ctggtccgcc tggacagcat caacggcgga tcggtcgaag acgccaagaa gcggcccgag ttcggcaaac tgacgccgtt gtaccccaac cagcggcttc gtctggaaac cagtaccgag cggctgacca cccgggtcat cgacctcatc atgccgatcg gcaagggtca acgcgcgttg attgtgtcgc cgcccaaagc gggcaagaca acgatcctgc aggacatcgc caacgcgatc accaggaaca acccggaatg ccacctcatg gtcgtgctcg tcgacgagcg gcctgaggag gtcaccgata tgcagcgctc ggtcaaaggc gaggtcatcg cttcaacttt cgaccggccg ccgtcggacc acacgtcggt cgccgagctg gcgatcgaac gcgccaagcg gctggtggag caaggcaagg acgtcgtggt gctgctcgat tcaatcaccc ggctaggccg cgcttacaac aacgcgtcgc cggcgtcggg ccggatcctg tccggtggtg tcgattccac ggcgttgtac ccgcccaagc gcttcctggg ggccgcgcgc aacatcgaag agggcgggtc gctgaccatc atcgccactg cgatggtcga gaccgggtcc actggtgaca cggtcatttt cgaggagttc aagggcaccg gcaacgccga gctcaagctg gaccgcaaga tcgccgagcg gcgggttttc cctgcggtcg acgtgaaccc ttctggaacc cgcaaggacg agctactgct gtcgcccgac gagttcgcta ttgtgcacaa gctgcgccgc gtgctatcgg gcctggattc ccaccaggcc atcgacctgc tgatgtcgca gctgcgtaag acgaagaaca actacgaatt ccttgttcag gtgtccaaga ccacgccagg gtccatggac agcgactaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for MytuD.17369.b.MB3.GE40925
HARVESTED ON: 9/23/2016
SEQUENCED ON: 9/9/2016
EXPECTED MW: 83kDa
OBSERVED MW: 85kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: Rosetta2 (DE3)
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 50
Validated AA Sequence
MNADTDYSIA EAAFNKGETA MTINGPWAWS NIDTSKVNYG VTVLPTFKGQ PSKPFVGVLS AGINAASPNK ELAKEFLENY LLTDEGLEAV NKDKPLGAVA LKSYEEELAK DPRIAATMEN AQKGEIMPNI PQMSAFWYAV RTAVINAASG RQTVDEALKD AQTNSSSNNN NNNNNNNLGI EGRISEGTLE AQTQGPGSRM VRKNGMRRGD AVTGAVRVPK EGEQPNQRQK FNPLVRLDSI NGGSVEDAKK RPEFGKLTPL YPNQRLRLET STERLTTRVI DLIMPIGKGQ RALIVSPPKA GKTTILQDIA NAITRNNPEC HLMVVLVDER PEEVTDMQRS VKGEVIASTF DRPPSDHTSV AELAIERAKR LVEQGKDV
Validated NT Sequence
gataacgctg gcgcgaaagc gggtctgacc ttcctggttg acctgattaa aaacaaacac atgaatgcag acaccgatta ctccatcgca gaagctgcct ttaataaagg cgaaacagcg atgaccatca acggcccgtg ggcatggtcc aacatcgaca ccagcaaagt gaattatggt gtaacggtac tgccgacctt caagggtcaa ccatccaaac cgttcgttgg cgtgctgagc gcaggtatta acgccgccag tccgaacaaa gagctggcaa aagagttcct cgaaaactat ctgctgactg atgaaggtct ggaagcggtt aataaagaca aaccgctggg tgccgtagcg ctgaagtctt acgaggaaga gttggcgaaa gatccacgta ttgccgccac tatggaaaac gcccagaaag gtgaaatcat gccgaacatc ccgcagatgt ccgctttctg gtatgccgtg cgtactgcgg tgatcaacgc cgccagcggt cgtcagactg tcgatgaagc cctgaaagac gcgcagacta attcgagctc gaacaacaac aacaacaaca ataacaacaa cctcgggatc gagggaagga tttcagaagg taccctggaa gctcagaccc agggtcctgg ttcgcgaatg gttcgtaaaa atggtatgcg tcgcggcgat gctgtcaccg gtgccgtgcg tgttccgaaa gaaggcgaac agccgaacca gcgtcaaaaa tttaatccgc tggtccgcct ggacagcatc aacggcggtt ctgtggaaga tgcgaaaaaa cgtccggaat tcggtaaact gaccccgctg tacccgaatc agcgtctgcg cctggaaacc agtacggaac gtctgaccac gcgcgttatc gatctgatta tgccgatcgg caagggtcag cgtgccctga ttgtgtcccc gccgaaagca ggcaaaacca cgattctgca agatatcgca aatgctatta cgcgcaacaa tccggaatgc catctgatgg tcgtgctggt tgacgaacgt ccggaagaag tcaccgatat gcagcgctca gttaaaggtg aagtcatcgc ctcgacgttt gaccgtccgc cgagtgatca caccagcgtg gcagaactgg ctattgaacg tgcgaaacgc ctggttgaac agggcaaaga cgttgtc
Expected Protein Sequence
MAHHHHHHMK IEEGKLVIWI NGDKGYNGLA EVGKKFEKDT GIKVTVEHPD KLEEKFPQVA ATGDGPDIIF WAHDRFGGYA QSGLLAEITP DKAFQDKLYP FTWDAVRYNG KLIAYPIAVE ALSLIYNKDL LPNPPKTWEE IPALDKELKA KGKSALMFNL QEPYFTWPLI AADGGYAFKY ENGKYDIKDV GVDNAGAKAG LTFLVDLIKN KHMNADTDYS IAEAAFNKGE TAMTINGPWA WSNIDTSKVN YGVTVLPTFK GQPSKPFVGV LSAGINAASP NKELAKEFLE NYLLTDEGLE AVNKDKPLGA VALKSYEEEL AKDPRIAATM ENAQKGEIMP NIPQMSAFWY AVRTAVINAA SGRQTVDEAL KDAQTNSSSN NNNNNNNNNL GIEGRISEGT LEAQTQGPGS MVRKNGMRRG DAVTGAVRVP KEGEQPNQRQ KFNPLVRLDS INGGSVEDAK KRPEFGKLTP LYPNQRLRLE TSTERLTTRV IDLIMPIGKG QRALIVSPPK AGKTTILQDI ANAITRNNPE CHLMVVLVDE RPEEVTDMQR SVKGEVIAST FDRPPSDHTS VAELAIERAK RLVEQGKDVV VLLDSITRLG RAYNNASPAS GRILSGGVDS TALYPPKRFL GAARNIEEGG SLTIIATAMV ETGSTGDTVI FEEFKGTGNA ELKLDRKIAE RRVFPAVDVN PSGTRKDELL LSPDEFAIVH KLRRVLSGLD SHQAIDLLMS QLRKTKNNYE FLVQVSKTTP GSMDSD
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcaca tgaaaatcga agaaggtaaa ctggtaatct ggattaacgg cgataaaggc tataacggtc tcgctgaagt cggtaagaaa ttcgagaaag ataccggaat taaagtcacc gttgagcatc cggataaact ggaagagaaa ttcccacagg ttgcggcaac tggcgatggc cctgacatta tcttctgggc acacgaccgc tttggtggct acgctcaatc tggcctgttg gctgaaatca ccccggacaa agcgttccag gacaagctgt atccgtttac ctgggatgcc gtacgttaca acggcaagct gattgcttac ccgatcgctg ttgaagcgtt atcgctgatt tataacaaag atctgctgcc gaacccgcca aaaacctggg aagagatccc ggcgctggat aaagaactga aagcgaaagg taagagcgcg ctgatgttca acctgcaaga accgtacttc acctggccgc tgattgctgc tgacgggggt tatgcgttca agtatgaaaa cggcaagtac gacattaaag acgtgggcgt ggataacgct ggcgcgaaag cgggtctgac cttcctggtt gacctgatta aaaacaaaca catgaatgca gacaccgatt actccatcgc agaagctgcc tttaataaag gcgaaacagc gatgaccatc aacggcccgt gggcatggtc caacatcgac accagcaaag tgaattatgg tgtaacggta ctgccgacct tcaagggtca accatccaaa ccgttcgttg gcgtgctgag cgcaggtatt aacgccgcca gtccgaacaa agagctggca aaagagttcc tcgaaaacta tctgctgact gatgaaggtc tggaagcggt taataaagac aaaccgctgg gtgccgtagc gctgaagtct tacgaggaag agttggcgaa agatccacgt attgccgcca ctatggaaaa cgcccagaaa ggtgaaatca tgccgaacat cccgcagatg tccgctttct ggtatgccgt gcgtactgcg gtgatcaacg ccgccagcgg tcgtcagact gtcgatgaag ccctgaaaga cgcgcagact aattcgagct cgaacaacaa caacaataac aataacaaca acctcgggat cgagggaagg atttcagaag gtaccctgga agctcagacc cagggtcctg gttcgatggt gcgcaagaac ggcatgcgcc gtggtgatgc ggtgaccggt gcggtgcggg tgcccaagga aggggagcaa cccaaccagc ggcagaagtt caacccgctg gtccgcctgg acagcatcaa cggcggatcg gtcgaagacg ccaagaagcg gcccgagttc ggcaaactga cgccgttgta ccccaaccag cggcttcgtc tggaaaccag taccgagcgg ctgaccaccc gggtcatcga cctcatcatg ccgatcggca agggtcaacg cgcgttgatt gtgtcgccgc ccaaagcggg caagacaacg atcctgcagg acatcgccaa cgcgatcacc aggaacaacc cggaatgcca cctcatggtc gtgctcgtcg acgagcggcc tgaggaggtc accgatatgc agcgctcggt caaaggcgag gtcatcgctt caactttcga ccggccgccg tcggaccaca cgtcggtcgc cgagctggcg atcgaacgcg ccaagcggct ggtggagcaa ggcaaggacg tcgtggtgct gctcgattca atcacccggc taggccgcgc ttacaacaac gcgtcgccgg cgtcgggccg gatcctgtcc ggtggtgtcg attccacggc gttgtacccg cccaagcgct tcctgggggc cgcgcgcaac atcgaagagg gcgggtcgct gaccatcatc gccactgcga tggtcgagac cgggtccact ggtgacacgg tcattttcga ggagttcaag ggcaccggca acgccgagct caagctggac cgcaagatcg ccgagcggcg ggttttccct gcggtcgacg tgaacccttc tggaacccgc aaggacgagc tactgctgtc gcccgacgag ttcgctattg tgcacaagct gcgccgcgtg ctatcgggcc tggattccca ccaggccatc gacctgctga tgtcgcagct gcgtaagacg aagaacaact acgaattcct tgttcaggtg tccaagacca cgccagggtc catggacagc gactaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for MytuD.17369.b.MB4.GE40926
HARVESTED ON: 9/23/2016
SEQUENCED ON: 9/9/2016
EXPECTED MW: 81kDa
OBSERVED MW: 80kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: Rosetta2 (DE3)
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 49
Validated AA Sequence
MNADTDYSIA EAAFNKGETA MTINGPWAWS NIDTSKVNYG VTVLPTFKGQ PSKPFVGVLS AGINAASPNK ELAKEFLENY LLTDEGLEAV NKDKPLGAVA LKSYEEELAK DPRIAATMEN AQKGEIMPNI PQMSAFWYAV RTAVINAASG RQTVDEALKD AQTNSSSNNN NNNNNNNLGI EGRISEGTLE AQTQGPGSRG AVRVPKEGEQ PNQRQKFNPL VRLDSINGGS VEDAKKRPEF GKLTPLYPNQ RLRLETSTER LTTRVIDLIM PIGKGQRALI VSPPKAGKTT ILQDIANAIT RNNPECHLMV VLVDERPEEV TDMQRSVKGE VIASTFDRPP SDHTSVAELA IERAKRLVEQ GKDV
Validated NT Sequence
gataacgctg gcgcgaaagc gggtctgacc ttcctggttg acctgattaa aaacaaacac atgaatgcag acaccgatta ctccatcgca gaagctgcct ttaataaagg cgaaacagcg atgaccatca acggcccgtg ggcatggtcc aacatcgaca ccagcaaagt gaattatggt gtaacggtac tgccgacctt caagggtcaa ccatccaaac cgttcgttgg cgtgctgagc gcaggtatta acgccgccag tccgaacaaa gagctggcaa aagagttcct cgaaaactat ctgctgactg atgaaggtct ggaagcggtt aataaagaca aaccgctggg tgccgtagcg ctgaagtctt acgaggaaga gttggcgaaa gatccacgta ttgccgccac tatggaaaac gcccagaaag gtgaaatcat gccgaacatc ccgcagatgt ccgctttctg gtatgccgtg cgtactgcgg tgatcaacgc cgccagcggt cgtcagactg tcgatgaagc cctgaaagac gcgcagacta attcgagctc gaacaacaac aacaacaaca ataacaacaa cctcgggatc gagggaagga tttcagaagg taccctggaa gctcagaccc agggtcctgg ttcgcgaggt gccgtgcgtg ttccgaaaga aggcgaacag ccgaaccagc gtcaaaaatt taatccgctg gtccgcctgg acagcatcaa cggcggttct gtggaagatg cgaaaaaacg tccggaattc ggtaaactga ccccgctgta cccgaatcag cgtctgcgcc tggaaaccag tacggaacgt ctgaccacgc gcgttatcga tctgattatg ccgatcggca agggtcagcg tgccctgatt gtgtccccgc cgaaagcagg caaaaccacg attctgcaag atatcgcaaa tgctattacg cgcaacaatc cggaatgcca tctgatggtc gtgctggttg acgaacgtcc ggaagaagtc accgatatgc agcgctcagt taaaggtgaa gtcatcgcct cgacgtttga ccgtccgccg agtgatcaca ccagcgtggc agaactggct attgaacgtg cgaaacgcct ggttgaacag ggcaaagacg ttg
Expected Protein Sequence
MAHHHHHHMK IEEGKLVIWI NGDKGYNGLA EVGKKFEKDT GIKVTVEHPD KLEEKFPQVA ATGDGPDIIF WAHDRFGGYA QSGLLAEITP DKAFQDKLYP FTWDAVRYNG KLIAYPIAVE ALSLIYNKDL LPNPPKTWEE IPALDKELKA KGKSALMFNL QEPYFTWPLI AADGGYAFKY ENGKYDIKDV GVDNAGAKAG LTFLVDLIKN KHMNADTDYS IAEAAFNKGE TAMTINGPWA WSNIDTSKVN YGVTVLPTFK GQPSKPFVGV LSAGINAASP NKELAKEFLE NYLLTDEGLE AVNKDKPLGA VALKSYEEEL AKDPRIAATM ENAQKGEIMP NIPQMSAFWY AVRTAVINAA SGRQTVDEAL KDAQTNSSSN NNNNNNNNNL GIEGRISEGT LEAQTQGPGS GAVRVPKEGE QPNQRQKFNP LVRLDSINGG SVEDAKKRPE FGKLTPLYPN QRLRLETSTE RLTTRVIDLI MPIGKGQRAL IVSPPKAGKT TILQDIANAI TRNNPECHLM VVLVDERPEE VTDMQRSVKG EVIASTFDRP PSDHTSVAEL AIERAKRLVE QGKDVVVLLD SITRLGRAYN NASPASGRIL SGGVDSTALY PPKRFLGAAR NIEEGGSLTI IATAMVETGS TGDTVIFEEF KGTGNAELKL DRKIAERRVF PAVDVNPSGT RKDELLLSPD EFAIVHKLRR VLSGLDSHQA IDLLMSQLRK TKNNYEFLVQ VSKTTPGSMD SD
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcaca tgaaaatcga agaaggtaaa ctggtaatct ggattaacgg cgataaaggc tataacggtc tcgctgaagt cggtaagaaa ttcgagaaag ataccggaat taaagtcacc gttgagcatc cggataaact ggaagagaaa ttcccacagg ttgcggcaac tggcgatggc cctgacatta tcttctgggc acacgaccgc tttggtggct acgctcaatc tggcctgttg gctgaaatca ccccggacaa agcgttccag gacaagctgt atccgtttac ctgggatgcc gtacgttaca acggcaagct gattgcttac ccgatcgctg ttgaagcgtt atcgctgatt tataacaaag atctgctgcc gaacccgcca aaaacctggg aagagatccc ggcgctggat aaagaactga aagcgaaagg taagagcgcg ctgatgttca acctgcaaga accgtacttc acctggccgc tgattgctgc tgacgggggt tatgcgttca agtatgaaaa cggcaagtac gacattaaag acgtgggcgt ggataacgct ggcgcgaaag cgggtctgac cttcctggtt gacctgatta aaaacaaaca catgaatgca gacaccgatt actccatcgc agaagctgcc tttaataaag gcgaaacagc gatgaccatc aacggcccgt gggcatggtc caacatcgac accagcaaag tgaattatgg tgtaacggta ctgccgacct tcaagggtca accatccaaa ccgttcgttg gcgtgctgag cgcaggtatt aacgccgcca gtccgaacaa agagctggca aaagagttcc tcgaaaacta tctgctgact gatgaaggtc tggaagcggt taataaagac aaaccgctgg gtgccgtagc gctgaagtct tacgaggaag agttggcgaa agatccacgt attgccgcca ctatggaaaa cgcccagaaa ggtgaaatca tgccgaacat cccgcagatg tccgctttct ggtatgccgt gcgtactgcg gtgatcaacg ccgccagcgg tcgtcagact gtcgatgaag ccctgaaaga cgcgcagact aattcgagct cgaacaacaa caacaataac aataacaaca acctcgggat cgagggaagg atttcagaag gtaccctgga agctcagacc cagggtcctg gttcgggtgc ggtgcgggtg cccaaggaag gggagcaacc caaccagcgg cagaagttca acccgctggt ccgcctggac agcatcaacg gcggatcggt cgaagacgcc aagaagcggc ccgagttcgg caaactgacg ccgttgtacc ccaaccagcg gcttcgtctg gaaaccagta ccgagcggct gaccacccgg gtcatcgacc tcatcatgcc gatcggcaag ggtcaacgcg cgttgattgt gtcgccgccc aaagcgggca agacaacgat cctgcaggac atcgccaacg cgatcaccag gaacaacccg gaatgccacc tcatggtcgt gctcgtcgac gagcggcctg aggaggtcac cgatatgcag cgctcggtca aaggcgaggt catcgcttca actttcgacc ggccgccgtc ggaccacacg tcggtcgccg agctggcgat cgaacgcgcc aagcggctgg tggagcaagg caaggacgtc gtggtgctgc tcgattcaat cacccggcta ggccgcgctt acaacaacgc gtcgccggcg tcgggccgga tcctgtccgg tggtgtcgat tccacggcgt tgtacccgcc caagcgcttc ctgggggccg cgcgcaacat cgaagagggc gggtcgctga ccatcatcgc cactgcgatg gtcgagaccg ggtccactgg tgacacggtc attttcgagg agttcaaggg caccggcaac gccgagctca agctggaccg caagatcgcc gagcggcggg ttttccctgc ggtcgacgtg aacccttctg gaacccgcaa ggacgagcta ctgctgtcgc ccgacgagtt cgctattgtg cacaagctgc gccgcgtgct atcgggcctg gattcccacc aggccatcga cctgctgatg tcgcagctgc gtaagacgaa gaacaactac gaattccttg ttcaggtgtc caagaccacg ccagggtcca tggacagcga ctaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa
Details for MytuD.17369.b.MB5.GE40927
HARVESTED ON: 9/23/2016
SEQUENCED ON: 9/9/2016
EXPECTED MW: 79kDa
OBSERVED MW: 80kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: Rosetta2 (DE3)
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 47
Validated AA Sequence
MNADTDYSIA EAAFNKGETA MTINGPWAWS NIDTSKVNYG VTVLPTFKGQ PSKPFVGVLS AGINAASPNK ELAKEFLENY LLTDEGLEAV NKDKPLGAVA LKSYEEELAK DPRIAATMEN AQKGEIMPNI PQMSAFWYAV RTAVINAASG RQTVDEALKD AQTNSSSNNN NNNNNNNLGI EGRISEGTLE AQTQGPGSRL VRLDSINGGS VEDAKKRPEF GKLTPLYPNQ RLRLETSTER LTTRVIDLIM PIGKGQRALI VSPPKAGKTT ILQDIANAIT RNNPECHLMV VLVDERPEEV TDMQRSVKGE VIASTFDRPP SDHTSVAELA IERAKRLVEQ GKDV
Validated NT Sequence
ataacgctgg cgcgaaagcg ggtctgacct tcctggttga cctgattaaa aacaaacaca tgaatgcaga caccgattac tccatcgcag aagctgcctt taataaaggc gaaacagcga tgaccatcaa cggcccgtgg gcatggtcca acatcgacac cagcaaagtg aattatggtg taacggtact gccgaccttc aagggtcaac catccaaacc gttcgttggc gtgctgagcg caggtattaa cgccgccagt ccgaacaaag agctggcaaa agagttcctc gaaaactatc tgctgactga tgaaggtctg gaagcggtta ataaagacaa accgctgggt gccgtagcgc tgaagtctta cgaggaagag ttggcgaaag atccacgtat tgccgccact atggaaaacg cccagaaagg tgaaatcatg ccgaacatcc cgcagatgtc cgctttctgg tatgccgtgc gtactgcggt gatcaacgcc gccagcggtc gtcagactgt cgatgaagcc ctgaaagacg cgcagactaa ttcgagctcg aacaacaaca acaacaacaa taacaacaac ctcgggatcg agggaaggat ttcagaaggt accctggaag ctcagaccca gggtcctggt tcgcgactgg tccgcctgga cagcatcaac ggcggttctg tggaagatgc gaaaaaacgt ccggaattcg gtaaactgac cccgctgtac ccgaatcagc gtctgcgcct ggaaaccagt acggaacgtc tgaccacgcg cgttatcgat ctgattatgc cgatcggcaa gggtcagcgt gccctgattg tgtccccgcc gaaagcaggc aaaaccacga ttctgcaaga tatcgcaaat gctattacgc gcaacaatcc ggaatgccat ctgatggtcg tgctggttga cgaacgtccg gaagaagtca ccgatatgca gcgctcagtt aaaggtgaag tcatcgcctc gacgtttgac cgtccgccga gtgatcacac cagcgtggca gaactggcta ttgaacgtgc gaaacgcctg gttgaacagg gcaaagacgt tgt
Expected Protein Sequence
MAHHHHHHMK IEEGKLVIWI NGDKGYNGLA EVGKKFEKDT GIKVTVEHPD KLEEKFPQVA ATGDGPDIIF WAHDRFGGYA QSGLLAEITP DKAFQDKLYP FTWDAVRYNG KLIAYPIAVE ALSLIYNKDL LPNPPKTWEE IPALDKELKA KGKSALMFNL QEPYFTWPLI AADGGYAFKY ENGKYDIKDV GVDNAGAKAG LTFLVDLIKN KHMNADTDYS IAEAAFNKGE TAMTINGPWA WSNIDTSKVN YGVTVLPTFK GQPSKPFVGV LSAGINAASP NKELAKEFLE NYLLTDEGLE AVNKDKPLGA VALKSYEEEL AKDPRIAATM ENAQKGEIMP NIPQMSAFWY AVRTAVINAA SGRQTVDEAL KDAQTNSSSN NNNNNNNNNL GIEGRISEGT LEAQTQGPGS LVRLDSINGG SVEDAKKRPE FGKLTPLYPN QRLRLETSTE RLTTRVIDLI MPIGKGQRAL IVSPPKAGKT TILQDIANAI TRNNPECHLM VVLVDERPEE VTDMQRSVKG EVIASTFDRP PSDHTSVAEL AIERAKRLVE QGKDVVVLLD SITRLGRAYN NASPASGRIL SGGVDSTALY PPKRFLGAAR NIEEGGSLTI IATAMVETGS TGDTVIFEEF KGTGNAELKL DRKIAERRVF PAVDVNPSGT RKDELLLSPD EFAIVHKLRR VLSGLDSHQA IDLLMSQLRK TKNNYEFLVQ VSKTTPGSMD SD
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcaca tgaaaatcga agaaggtaaa ctggtaatct ggattaacgg cgataaaggc tataacggtc tcgctgaagt cggtaagaaa ttcgagaaag ataccggaat taaagtcacc gttgagcatc cggataaact ggaagagaaa ttcccacagg ttgcggcaac tggcgatggc cctgacatta tcttctgggc acacgaccgc tttggtggct acgctcaatc tggcctgttg gctgaaatca ccccggacaa agcgttccag gacaagctgt atccgtttac ctgggatgcc gtacgttaca acggcaagct gattgcttac ccgatcgctg ttgaagcgtt atcgctgatt tataacaaag atctgctgcc gaacccgcca aaaacctggg aagagatccc ggcgctggat aaagaactga aagcgaaagg taagagcgcg ctgatgttca acctgcaaga accgtacttc acctggccgc tgattgctgc tgacgggggt tatgcgttca agtatgaaaa cggcaagtac gacattaaag acgtgggcgt ggataacgct ggcgcgaaag cgggtctgac cttcctggtt gacctgatta aaaacaaaca catgaatgca gacaccgatt actccatcgc agaagctgcc tttaataaag gcgaaacagc gatgaccatc aacggcccgt gggcatggtc caacatcgac accagcaaag tgaattatgg tgtaacggta ctgccgacct tcaagggtca accatccaaa ccgttcgttg gcgtgctgag cgcaggtatt aacgccgcca gtccgaacaa agagctggca aaagagttcc tcgaaaacta tctgctgact gatgaaggtc tggaagcggt taataaagac aaaccgctgg gtgccgtagc gctgaagtct tacgaggaag agttggcgaa agatccacgt attgccgcca ctatggaaaa cgcccagaaa ggtgaaatca tgccgaacat cccgcagatg tccgctttct ggtatgccgt gcgtactgcg gtgatcaacg ccgccagcgg tcgtcagact gtcgatgaag ccctgaaaga cgcgcagact aattcgagct cgaacaacaa caacaataac aataacaaca acctcgggat cgagggaagg atttcagaag gtaccctgga agctcagacc cagggtcctg gttcgctggt ccgcctggac agcatcaacg gcggatcggt cgaagacgcc aagaagcggc ccgagttcgg caaactgacg ccgttgtacc ccaaccagcg gcttcgtctg gaaaccagta ccgagcggct gaccacccgg gtcatcgacc tcatcatgcc gatcggcaag ggtcaacgcg cgttgattgt gtcgccgccc aaagcgggca agacaacgat cctgcaggac atcgccaacg cgatcaccag gaacaacccg gaatgccacc tcatggtcgt gctcgtcgac gagcggcctg aggaggtcac cgatatgcag cgctcggtca aaggcgaggt catcgcttca actttcgacc ggccgccgtc ggaccacacg tcggtcgccg agctggcgat cgaacgcgcc aagcggctgg tggagcaagg caaggacgtc gtggtgctgc tcgattcaat cacccggcta ggccgcgctt acaacaacgc gtcgccggcg tcgggccgga tcctgtccgg tggtgtcgat tccacggcgt tgtacccgcc caagcgcttc ctgggggccg cgcgcaacat cgaagagggc gggtcgctga ccatcatcgc cactgcgatg gtcgagaccg ggtccactgg tgacacggtc attttcgagg agttcaaggg caccggcaac gccgagctca agctggaccg caagatcgcc gagcggcggg ttttccctgc ggtcgacgtg aacccttctg gaacccgcaa ggacgagcta ctgctgtcgc ccgacgagtt cgctattgtg cacaagctgc gccgcgtgct atcgggcctg gattcccacc aggccatcga cctgctgatg tcgcagctgc gtaagacgaa gaacaactac gaattccttg ttcaggtgtc caagaccacg ccagggtcca tggacagcga ctaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa
Details for MytuD.17369.b.MB6.GE40928
HARVESTED ON: 9/23/2016
SEQUENCED ON: 9/9/2016
EXPECTED MW: 78kDa
OBSERVED MW: 80kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: Rosetta2 (DE3)
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 46
Validated AA Sequence
MNADTDYSIA EAAFNKGETA MTINGPWAWS NIDTSKVNYG VTVLPTFKGQ PSKPFVGVLS AGINAASPNK ELAKEFLENY LLTDEGLEAV NKDKPLGAVA LKSYEEELAK DPRIAATMEN AQKGEIMPNI PQMSAFWYAV RTAVINAASG RQTVDEALKD AQTNSSSNNN NNNNNNNLGI EGRISEGTLE AQTQGPGSRG SVEDAKKRPE FGKLTPLYPN QRLRLETSTE RLTTRVIDLI MPIGKGQRAL IVSPPKAGKT TILQDIANAI TRNNPECHLM VVLVDERPEE VTDMQRSVKG EVIASTFDRP PSDHTSVAEL AIERAKRLVE QGKDV
Validated NT Sequence
gataacgctg gcgcgaaagc gggtctgacc ttcctggttg acctgattaa aaacaaacac atgaatgcag acaccgatta ctccatcgca gaagctgcct ttaataaagg cgaaacagcg atgaccatca acggcccgtg ggcatggtcc aacatcgaca ccagcaaagt gaattatggt gtaacggtac tgccgacctt caagggtcaa ccatccaaac cgttcgttgg cgtgctgagc gcaggtatta acgccgccag tccgaacaaa gagctggcaa aagagttcct cgaaaactat ctgctgactg atgaaggtct ggaagcggtt aataaagaca aaccgctggg tgccgtagcg ctgaagtctt acgaggaaga gttggcgaaa gatccacgta ttgccgccac tatggaaaac gcccagaaag gtgaaatcat gccgaacatc ccgcagatgt ccgctttctg gtatgccgtg cgtactgcgg tgatcaacgc cgccagcggt cgtcagactg tcgatgaagc cctgaaagac gcgcagacta attcgagctc gaacaacaac aacaacaaca ataacaacaa cctcgggatc gagggaagga tttcagaagg taccctggaa gctcagaccc agggtcctgg ttcgcgaggt tctgtggaag atgcgaaaaa acgtccggaa ttcggtaaac tgaccccgct gtacccgaat cagcgtctgc gcctggaaac cagtacggaa cgtctgacca cgcgcgttat cgatctgatt atgccgatcg gcaagggtca gcgtgccctg attgtgtccc cgccgaaagc aggcaaaacc acgattctgc aagatatcgc aaatgctatt acgcgcaaca atccggaatg ccatctgatg gtcgtgctgg ttgacgaacg tccggaagaa gtcaccgata tgcagcgctc agttaaaggt gaagtcatcg cctcgacgtt tgaccgtccg ccgagtgatc acaccagcgt ggcagaactg gctattgaac gtgcgaaacg cctggttgaa cagggcaaag acgttg
Expected Protein Sequence
MAHHHHHHMK IEEGKLVIWI NGDKGYNGLA EVGKKFEKDT GIKVTVEHPD KLEEKFPQVA ATGDGPDIIF WAHDRFGGYA QSGLLAEITP DKAFQDKLYP FTWDAVRYNG KLIAYPIAVE ALSLIYNKDL LPNPPKTWEE IPALDKELKA KGKSALMFNL QEPYFTWPLI AADGGYAFKY ENGKYDIKDV GVDNAGAKAG LTFLVDLIKN KHMNADTDYS IAEAAFNKGE TAMTINGPWA WSNIDTSKVN YGVTVLPTFK GQPSKPFVGV LSAGINAASP NKELAKEFLE NYLLTDEGLE AVNKDKPLGA VALKSYEEEL AKDPRIAATM ENAQKGEIMP NIPQMSAFWY AVRTAVINAA SGRQTVDEAL KDAQTNSSSN NNNNNNNNNL GIEGRISEGT LEAQTQGPGS GSVEDAKKRP EFGKLTPLYP NQRLRLETST ERLTTRVIDL IMPIGKGQRA LIVSPPKAGK TTILQDIANA ITRNNPECHL MVVLVDERPE EVTDMQRSVK GEVIASTFDR PPSDHTSVAE LAIERAKRLV EQGKDVVVLL DSITRLGRAY NNASPASGRI LSGGVDSTAL YPPKRFLGAA RNIEEGGSLT IIATAMVETG STGDTVIFEE FKGTGNAELK LDRKIAERRV FPAVDVNPSG TRKDELLLSP DEFAIVHKLR RVLSGLDSHQ AIDLLMSQLR KTKNNYEFLV QVSKTTPGSM DSD
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcaca tgaaaatcga agaaggtaaa ctggtaatct ggattaacgg cgataaaggc tataacggtc tcgctgaagt cggtaagaaa ttcgagaaag ataccggaat taaagtcacc gttgagcatc cggataaact ggaagagaaa ttcccacagg ttgcggcaac tggcgatggc cctgacatta tcttctgggc acacgaccgc tttggtggct acgctcaatc tggcctgttg gctgaaatca ccccggacaa agcgttccag gacaagctgt atccgtttac ctgggatgcc gtacgttaca acggcaagct gattgcttac ccgatcgctg ttgaagcgtt atcgctgatt tataacaaag atctgctgcc gaacccgcca aaaacctggg aagagatccc ggcgctggat aaagaactga aagcgaaagg taagagcgcg ctgatgttca acctgcaaga accgtacttc acctggccgc tgattgctgc tgacgggggt tatgcgttca agtatgaaaa cggcaagtac gacattaaag acgtgggcgt ggataacgct ggcgcgaaag cgggtctgac cttcctggtt gacctgatta aaaacaaaca catgaatgca gacaccgatt actccatcgc agaagctgcc tttaataaag gcgaaacagc gatgaccatc aacggcccgt gggcatggtc caacatcgac accagcaaag tgaattatgg tgtaacggta ctgccgacct tcaagggtca accatccaaa ccgttcgttg gcgtgctgag cgcaggtatt aacgccgcca gtccgaacaa agagctggca aaagagttcc tcgaaaacta tctgctgact gatgaaggtc tggaagcggt taataaagac aaaccgctgg gtgccgtagc gctgaagtct tacgaggaag agttggcgaa agatccacgt attgccgcca ctatggaaaa cgcccagaaa ggtgaaatca tgccgaacat cccgcagatg tccgctttct ggtatgccgt gcgtactgcg gtgatcaacg ccgccagcgg tcgtcagact gtcgatgaag ccctgaaaga cgcgcagact aattcgagct cgaacaacaa caacaataac aataacaaca acctcgggat cgagggaagg atttcagaag gtaccctgga agctcagacc cagggtcctg gttcgggatc ggtcgaagac gccaagaagc ggcccgagtt cggcaaactg acgccgttgt accccaacca gcggcttcgt ctggaaacca gtaccgagcg gctgaccacc cgggtcatcg acctcatcat gccgatcggc aagggtcaac gcgcgttgat tgtgtcgccg cccaaagcgg gcaagacaac gatcctgcag gacatcgcca acgcgatcac caggaacaac ccggaatgcc acctcatggt cgtgctcgtc gacgagcggc ctgaggaggt caccgatatg cagcgctcgg tcaaaggcga ggtcatcgct tcaactttcg accggccgcc gtcggaccac acgtcggtcg ccgagctggc gatcgaacgc gccaagcggc tggtggagca aggcaaggac gtcgtggtgc tgctcgattc aatcacccgg ctaggccgcg cttacaacaa cgcgtcgccg gcgtcgggcc ggatcctgtc cggtggtgtc gattccacgg cgttgtaccc gcccaagcgc ttcctggggg ccgcgcgcaa catcgaagag ggcgggtcgc tgaccatcat cgccactgcg atggtcgaga ccgggtccac tggtgacacg gtcattttcg aggagttcaa gggcaccggc aacgccgagc tcaagctgga ccgcaagatc gccgagcggc gggttttccc tgcggtcgac gtgaaccctt ctggaacccg caaggacgag ctactgctgt cgcccgacga gttcgctatt gtgcacaagc tgcgccgcgt gctatcgggc ctggattccc accaggccat cgacctgctg atgtcgcagc tgcgtaagac gaagaacaac tacgaattcc ttgttcaggt gtccaagacc acgccagggt ccatggacag cgactaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for MytuD.17369.b.MB7.GE40929
HARVESTED ON: 9/23/2016
SEQUENCED ON: 9/9/2016
EXPECTED MW: 77kDa
OBSERVED MW: 79kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: Rosetta2 (DE3)
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 77
Validated AA Sequence
MFNLQEPYFT WPLIAADGGY AFKYENGKYD IKDVGVDNAG AKAGLTFLVD LIKNKHMNAD TDYSIAEAAF NKGETAMTIN GPWAWSNIDT SKVNYGVTVL PTFKGQPSKP FVGVLSAGIN AASPNKELAK EFLENYLLTD EGLEAVNKDK PLGAVALKSY EEELAKDPRI AATMENAQKG EIMPNIPQMS AFWYAVRTAV INAASGRQTV DEALKDAQTN SSSNNNNNNN NNNLGIEGRI SEGTLEAQTQ GPGSRGKLTP LYPNQRLRLE TSTERLTTRV IDLIMPIGKG QRALIVSPPK AGKTTILQDI ANAITRNNPE CHLMVVLVDE RPEEVTDMQR SVKGEVIAST FDRPPSDHTS VAELAIERAK RLVEQGKDVV VLLDSITRLG RAYNNASPAS GRILSGGVDS TALYPPKRFL GAARNIEEGG SLTIIATAMV ETGSTGDTVI FEEFKGTGNA ELKLDRKIAE RRVFPAVDVN PSGTRKDELL LSPDEFAIVH KLRRVLSGLD SHQAIDLLMS QLRKTKNNYE FLVQVSKTTP GSMDS
Validated NT Sequence
tgaaaatcga agaaggtaaa ctggtaatct ggattaacgg cgataaaggc tataacggtc tcgctgaagt cggtaagaaa ttcgagaaag ataccggaat taaagtcacc gttgagcatc cggataaact ggaagagaaa ttcccacagg ttgcggcaac tggcgatggc cctgacatta tcttctgggc acacgaccgc tttggtggct acgctcaatc tggcctgttg gctgaaatca ccccggacaa agcgttccag gacaagctgt atccgtttac ctgggatgcc gtacgttaca acggcaagct gattgcttac ccgatcgctg ttgaagcgtt atcgctgatt tataacaaag atctgctgcc gaacccgcca aaaacctggg aagagatccc ggcgctggat aaagaactga aagcgaaagg taagagcgcg ctgatgttca acctgcaaga accgtacttc acctggccgc tgattgctgc tgacgggggt tatgcgttca agtatgaaaa cggcaagtac gacattaaag acgtgggcgt ggataacgct ggcgcgaaag cgggtctgac cttcctggtt gacctgatta aaaacaaaca catgaatgca gacaccgatt actccatcgc agaagctgcc tttaataaag gcgaaacagc gatgaccatc aacggcccgt gggcatggtc caacatcgac accagcaaag tgaattatgg tgtaacggta ctgccgacct tcaagggtca accatccaaa ccgttcgttg gcgtgctgag cgcaggtatt aacgccgcca gtccgaacaa agagctggca aaagagttcc tcgaaaacta tctgctgact gatgaaggtc tggaagcggt taataaagac aaaccgctgg gtgccgtagc gctgaagtct tacgaggaag agttggcgaa agatccacgt attgccgcca ctatggaaaa cgcccagaaa ggtgaaatca tgccgaacat cccgcagatg tccgctttct ggtatgccgt gcgtactgcg gtgatcaacg ccgccagcgg tcgtcagact gtcgatgaag ccctgaaaga cgcgcagact aattcgagct cgaacaacaa caacaacaac aataacaaca acctcgggat cgagggaagg atttcagaag gtaccctgga agctcagacc cagggtcctg gttcgcgagg taaactgacc ccgctgtacc cgaatcagcg tctgcgcctg gaaaccagta cggaacgtct gaccacgcgc gttatcgatc tgattatgcc gatcggcaag ggtcagcgtg ccctgattgt gtccccgccg aaagcaggca aaaccacgat tctgcaagat atcgcaaatg ctattacgcg caacaatccg gaatgccatc tgatggtcgt gctggttgac gaacgtccgg aagaagtcac cgatatgcag cgctcagtta aaggtgaagt catcgcctcg acgtttgacc gtccgccgag tgatcacacc agcgtggcag aactggctat tgaacgtgcg aaacgcctgg ttgaacaggg caaagacgtt gtcgtgctgc tggatagcat cacgcgtctg ggtcgcgcct acaacaatgc gagcccggca tctggtcgca ttctgagtgg cggtgttgat tccaccgcac tgtatccgcc gaaacgtttc ctgggtgccg cacgtaacat tgaagaaggc ggttcactga cgattatcgc gaccgccatg gtggaaaccg gttcgacggg cgataccgtt atctttgaag aattcaaagg caccggtaac gcggaactga aactggaccg taaaattgct gaacgtcgcg tgtttccggc ggtcgatgtg aatccgagcg gtacccgcaa agacgaactg ctgctgtctc cggatgaatt cgcaatcgtc cataaactgc gtcgcgtgct gtcaggcctg gactcgcacc aggcgattga tctgctgatg agccaactgc gcaaaaccaa aaataactac gaatttctgg ttcaagtgtc aaaaacgacg ccgggctcaa tggatagcg
Expected Protein Sequence
MAHHHHHHMK IEEGKLVIWI NGDKGYNGLA EVGKKFEKDT GIKVTVEHPD KLEEKFPQVA ATGDGPDIIF WAHDRFGGYA QSGLLAEITP DKAFQDKLYP FTWDAVRYNG KLIAYPIAVE ALSLIYNKDL LPNPPKTWEE IPALDKELKA KGKSALMFNL QEPYFTWPLI AADGGYAFKY ENGKYDIKDV GVDNAGAKAG LTFLVDLIKN KHMNADTDYS IAEAAFNKGE TAMTINGPWA WSNIDTSKVN YGVTVLPTFK GQPSKPFVGV LSAGINAASP NKELAKEFLE NYLLTDEGLE AVNKDKPLGA VALKSYEEEL AKDPRIAATM ENAQKGEIMP NIPQMSAFWY AVRTAVINAA SGRQTVDEAL KDAQTNSSSN NNNNNNNNNL GIEGRISEGT LEAQTQGPGS GKLTPLYPNQ RLRLETSTER LTTRVIDLIM PIGKGQRALI VSPPKAGKTT ILQDIANAIT RNNPECHLMV VLVDERPEEV TDMQRSVKGE VIASTFDRPP SDHTSVAELA IERAKRLVEQ GKDVVVLLDS ITRLGRAYNN ASPASGRILS GGVDSTALYP PKRFLGAARN IEEGGSLTII ATAMVETGST GDTVIFEEFK GTGNAELKLD RKIAERRVFP AVDVNPSGTR KDELLLSPDE FAIVHKLRRV LSGLDSHQAI DLLMSQLRKT KNNYEFLVQV SKTTPGSMDS D
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcaca tgaaaatcga agaaggtaaa ctggtaatct ggattaacgg cgataaaggc tataacggtc tcgctgaagt cggtaagaaa ttcgagaaag ataccggaat taaagtcacc gttgagcatc cggataaact ggaagagaaa ttcccacagg ttgcggcaac tggcgatggc cctgacatta tcttctgggc acacgaccgc tttggtggct acgctcaatc tggcctgttg gctgaaatca ccccggacaa agcgttccag gacaagctgt atccgtttac ctgggatgcc gtacgttaca acggcaagct gattgcttac ccgatcgctg ttgaagcgtt atcgctgatt tataacaaag atctgctgcc gaacccgcca aaaacctggg aagagatccc ggcgctggat aaagaactga aagcgaaagg taagagcgcg ctgatgttca acctgcaaga accgtacttc acctggccgc tgattgctgc tgacgggggt tatgcgttca agtatgaaaa cggcaagtac gacattaaag acgtgggcgt ggataacgct ggcgcgaaag cgggtctgac cttcctggtt gacctgatta aaaacaaaca catgaatgca gacaccgatt actccatcgc agaagctgcc tttaataaag gcgaaacagc gatgaccatc aacggcccgt gggcatggtc caacatcgac accagcaaag tgaattatgg tgtaacggta ctgccgacct tcaagggtca accatccaaa ccgttcgttg gcgtgctgag cgcaggtatt aacgccgcca gtccgaacaa agagctggca aaagagttcc tcgaaaacta tctgctgact gatgaaggtc tggaagcggt taataaagac aaaccgctgg gtgccgtagc gctgaagtct tacgaggaag agttggcgaa agatccacgt attgccgcca ctatggaaaa cgcccagaaa ggtgaaatca tgccgaacat cccgcagatg tccgctttct ggtatgccgt gcgtactgcg gtgatcaacg ccgccagcgg tcgtcagact gtcgatgaag ccctgaaaga cgcgcagact aattcgagct cgaacaacaa caacaataac aataacaaca acctcgggat cgagggaagg atttcagaag gtaccctgga agctcagacc cagggtcctg gttcgggcaa actgacgccg ttgtacccca accagcggct tcgtctggaa accagtaccg agcggctgac cacccgggtc atcgacctca tcatgccgat cggcaagggt caacgcgcgt tgattgtgtc gccgcccaaa gcgggcaaga caacgatcct gcaggacatc gccaacgcga tcaccaggaa caacccggaa tgccacctca tggtcgtgct cgtcgacgag cggcctgagg aggtcaccga tatgcagcgc tcggtcaaag gcgaggtcat cgcttcaact ttcgaccggc cgccgtcgga ccacacgtcg gtcgccgagc tggcgatcga acgcgccaag cggctggtgg agcaaggcaa ggacgtcgtg gtgctgctcg attcaatcac ccggctaggc cgcgcttaca acaacgcgtc gccggcgtcg ggccggatcc tgtccggtgg tgtcgattcc acggcgttgt acccgcccaa gcgcttcctg ggggccgcgc gcaacatcga agagggcggg tcgctgacca tcatcgccac tgcgatggtc gagaccgggt ccactggtga cacggtcatt ttcgaggagt tcaagggcac cggcaacgcc gagctcaagc tggaccgcaa gatcgccgag cggcgggttt tccctgcggt cgacgtgaac ccttctggaa cccgcaagga cgagctactg ctgtcgcccg acgagttcgc tattgtgcac aagctgcgcc gcgtgctatc gggcctggat tcccaccagg ccatcgacct gctgatgtcg cagctgcgta agacgaagaa caactacgaa ttccttgttc aggtgtccaa gaccacgcca gggtccatgg acagcgacta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a