MytuD.17366.a

Chaperone protein DnaJ2 (Rv2373c)

CENTER ID: MytuD.17366.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.17366.a.AE1.GE44130 full length 1 382
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.2654
TubercuList: Rv2373c
MTB Network Portal: Rv2373c
UniProt: P9WNV7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MARDYYGLLG VSKNASDADI KRAYRKLARE LHPDVNPDEA AQAKFKEISV AYEVLSDPDK RRIVDLGGDP LESAAAGGNG FGGFGGLGDV FEAFFGGGFG GGAASRGPIG RVRPGSDSLL RMRLDLEECA TGVTKQVTVD TAVLCDRCQG KGTNGDSVPI PCDTCGGRGE VQTVQRSLLG QMLTSRPCPT CRGVGVVIPD PCQQCMGDGR IRARREISVK IPAGVGDGMR VRLAAQGEVG PGGGPAGDLY VEVHEQAHDV FVREGDHLHC TVSVPMVDAA LGVTVTVDAI LDGLSEITIP PGTQPGSVIT LRGRGMPHLR SNTRGDLHVH VEVVVPTRLD HQDIELLREL KGRRDREVAE VRSTHAAAGG LFSRLRETFT GR
NT Sequence
gtggcacgcg attattacgg gctgctcggc gtgagcaaga acgccagcga cgcggacatc aaacgcgcct accgcaagct ggcgcgcgag ctgcatcccg acgtcaaccc ggacgaggct gcgcaggcga aattcaaaga aatcagcgtc gcctacgagg tgctcagtga cccggacaaa cgtcgcatcg tcgacctggg cggggatccg ctggagagcg ccgctgcggg cggcaatggg ttcggtggct tcggcggcct cggcgacgtg ttcgaggcgt tctttggcgg gggtttcggt gggggcgcgg cgtcccgagg gccgatcggc cgggtccggc cgggttcgga ctcgctgcta cgaatgcggc tggatctcga agagtgcgca acaggtgtca ccaagcaggt caccgtcgat accgcggtgt tgtgcgaccg gtgccagggc aagggcacca acggcgattc ggttccgata ccctgcgaca cctgcggtgg ccgcggggag gtgcagaccg tgcagcgatc gctgttgggt cagatgttga cgtcgcggcc gtgtcccacc tgccgcggcg tcggggtggt tatccccgac ccgtgccagc aatgcatggg cgatggccgg atccgggccc gtcgggagat cagcgtcaag atcccggccg gtgtcggcga cgggatgcga gttcggctcg ccgctcaggg cgaggtcggg cccgggggag ggccggcggg tgacctctac gtcgaggtcc atgagcaggc gcacgacgtc tttgtccgcg aaggtgacca tctgcattgc acggtttcgg tgcccatggt cgacgcggcg ctgggtgtta cggtcacggt ggacgccatc ctggacggcc tgagcgagat caccattcca cccggcacgc agccaggttc ggtgatcacg ctgcgcggtc gaggaatgcc gcacctgcgt tccaacacgc gtggcgacct gcacgttcac gtggaggtgg tggtcccgac ccggctggat caccaggaca tcgaactgct gcgcgagctg aagggtcgcc gcgaccgcga ggtggccgag gtccgttcga cccacgccgc cgccggcgga ctgttcagcc ggttgcgcga gaccttcacc gggcgctaa
Details for MytuD.17366.a.AE1.GE44130
HARVESTED ON: 3/2/2021
SEQUENCED ON: 3/10/2021
EXPECTED MW: 41kDa
OBSERVED MW: 41kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MVARDYYGLL GVSKNASDAD IKRAYRKLAR ELHPDVNPDE AAQAKFKEIS VAYEVLSDPD KRRIVDLGGD PLESAAAGGN GFGGFGGLGD VFEAFFGGGF GGGAASRGPI GRVRPGSDSL LRMRLDLEEC ATGVTKQVTV DTAVLCDRCQ GKGTNGDSVP IPCDTCGGRG EVQTVQRSLL GQMLTSRPCP TCRGVGVVIP DPCQQCMGDG RIRARREISV KIPAGVGDGM RVRLAAQGEV GPGGGPAGDL YVEVHEQAHD VFVREGDHLH CTVSVPMVDA ALGVTVTVDA ILDGLSEITI PPGTQPGSVI TLRGRGMPHL RSNTRGDLHV HVEVVVPTRL DHQDIELLRE LKGRRDREVA EVRSTHAAAG GLFSRLRETF TGRAGHHHHH H
Validated NT Sequence
tctctcccat ggtggcacgc gattattacg ggctgctcgg cgtgagcaag aacgccagcg acgcggacat caaacgcgcc taccgcaagc tggcgcgcga gctgcatccc gacgtcaacc cggacgaggc tgcgcaggcg aaattcaaag aaatcagcgt cgcctacgag gtgctcagtg acccggacaa acgtcgcatc gtcgacctgg gcggggatcc gctggagagc gccgctgcgg gcggcaatgg gttcggtggc ttcggcggcc tcggcgacgt gttcgaggcg ttctttggcg ggggtttcgg tgggggcgcg gcgtcccgag ggccgatcgg ccgggtccgg ccgggttcgg actcgctgct acgaatgcgg ctggatctcg aagagtgcgc aacaggtgtc accaagcagg tcaccgtcga taccgcggtg ttgtgcgacc ggtgccaggg caagggcacc aacggcgatt cggttccgat accctgcgac acctgcggtg gccgcgggga ggtgcagacc gtgcagcgat cgctgttggg tcagatgttg acgtcgcggc cgtgtcccac ctgccgcggc gtcggggtgg ttatccccga cccgtgccag caatgcatgg gcgatggccg gatccgggcc cgtcgggaga tcagcgtcaa gatcccggcc ggtgtcggcg acgggatgcg agttcggctc gccgctcagg gcgaggtcgg gcccggggga gggccggcgg gtgacctcta cgtcgaggtc catgagcagg cgcacgacgt ctttgtccgc gaaggtgacc atctgcattg cacggtttcg gtgcccatgg tcgacgcggc gctgggtgtt acggtcacgg tggacgccat cctggacggc ctgagcgaga tcaccattcc acccggcacg cagccaggtt cggtgatcac gctgcgcggt cgaggaatgc cgcacctgcg ttccaacacg cgtggcgacc tgcacgttca cgtggaggtg gtggtcccga cccggctgga tcaccaggac atcgaactgc tgcgcgagct gaagggtcgc cgcgaccgcg aggtggccga ggtccgttcg acccacgccg ccgccggcgg actgttcagc cggttgcgcg agaccttcac cgggcgcgct gggcaccacc atcatcatca ttaacgga
Expected Protein Sequence
MARDYYGLLG VSKNASDADI KRAYRKLARE LHPDVNPDEA AQAKFKEISV AYEVLSDPDK RRIVDLGGDP LESAAAGGNG FGGFGGLGDV FEAFFGGGFG GGAASRGPIG RVRPGSDSLL RMRLDLEECA TGVTKQVTVD TAVLCDRCQG KGTNGDSVPI PCDTCGGRGE VQTVQRSLLG QMLTSRPCPT CRGVGVVIPD PCQQCMGDGR IRARREISVK IPAGVGDGMR VRLAAQGEVG PGGGPAGDLY VEVHEQAHDV FVREGDHLHC TVSVPMVDAA LGVTVTVDAI LDGLSEITIP PGTQPGSVIT LRGRGMPHLR SNTRGDLHVH VEVVVPTRLD HQDIELLREL KGRRDREVAE VRSTHAAAGG LFSRLRETFT GRGHHHHHH
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atttgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat cgatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt taagaaggag tctctcccgt ggcacgcgat tattacgggc tgctcggcgt gagcaagaac gccagcgacg cggacatcaa acgcgcctac cgcaagctgg cgcgcgagct gcatcccgac gtcaacccgg acgaggctgc gcaggcgaaa ttcaaagaaa tcagcgtcgc ctacgaggtg ctcagtgacc cggacaaacg tcgcatcgtc gacctgggcg gggatccgct ggagagcgcc gctgcgggcg gcaatgggtt cggtggcttc ggcggcctcg gcgacgtgtt cgaggcgttc tttggcgggg gtttcggtgg gggcgcggcg tcccgagggc cgatcggccg ggtccggccg ggttcggact cgctgctacg aatgcggctg gatctcgaag agtgcgcaac aggtgtcacc aagcaggtca ccgtcgatac cgcggtgttg tgcgaccggt gccagggcaa gggcaccaac ggcgattcgg ttccgatacc ctgcgacacc tgcggtggcc gcggggaggt gcagaccgtg cagcgatcgc tgttgggtca gatgttgacg tcgcggccgt gtcccacctg ccgcggcgtc ggggtggtta tccccgaccc gtgccagcaa tgcatgggcg atggccggat ccgggcccgt cgggagatca gcgtcaagat cccggccggt gtcggcgacg ggatgcgagt tcggctcgcc gctcagggcg aggtcgggcc cgggggaggg ccggcgggtg acctctacgt cgaggtccat gagcaggcgc acgacgtctt tgtccgcgaa ggtgaccatc tgcattgcac ggtttcggtg cccatggtcg acgcggcgct gggtgttacg gtcacggtgg acgccatcct ggacggcctg agcgagatca ccattccacc cggcacgcag ccaggttcgg tgatcacgct gcgcggtcga ggaatgccgc acctgcgttc caacacgcgt ggcgacctgc acgttcacgt ggaggtggtg gtcccgaccc ggctggatca ccaggacatc gaactgctgc gcgagctgaa gggtcgccgc gaccgcgagg tggccgaggt ccgttcgacc cacgccgccg ccggcggact gttcagccgg ttgcgcgaga ccttcaccgg gcgcgggcac caccatcatc atcattaacg gatccgaatt cgagctccgt cgacaagctt gcggccgcac tcgagcacca ccaccaccac cactgagatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg 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