MytuD.17328.a

IS1560, transposase

CENTER ID: MytuD.17328.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

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Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.17328.a.A1.PS01036 Full length( MytuD.17328.a ) 1 234

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.3782
RefSeq: NP_217903.1
TubercuList: Rv3386
MTB Network Portal: Rv3386
UniProt: O50413

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFRTVGDQAS LWESVLPEEL RRLPEELARV DALLDDSAFF CPFVPFFDPR MGRPSIPMET YLRLMFLKFR YRLGYESLCR EVTDSITWRR FCRIPLEGSV PHPTTLMKLT TRCGEDAVAG LNEALLAKAA SEKLLRTNKV RADTTVVEGD VGYPTDTGLL AKAVGSMART VARIKAADAG SAPLGGSSGP RDRLQAAVTR RAATRSGAGL RAPDHRGASR DRRAGADRGC RGGT
NT Sequence
GTGTTTCGTA CTGTAGGCGA TCAGGCATCG TTGTGGGAAT CCGTGCTGCC CGAGGAGTTG CGGCGGCTGC CCGAAGAGCT GGCCCGGGTG GATGCGCTGC TCGATGATTC GGCGTTCTTC TGCCCGTTTG TGCCGTTCTT CGACCCGCGG ATGGGTCGGC CGTCCATACC GATGGAGACC TATTTGCGGT TGATGTTCTT GAAGTTCCGT TACCGGTTGG GCTATGAGTC GCTGTGTCGG GAGGTCACCG ATTCGATCAC CTGGCGGCGG TTCTGCCGTA TTCCGTTGGA GGGATCGGTG CCGCACCCAA CCACGTTGAT GAAGCTGACC ACGCGCTGCG GTGAGGATGC GGTGGCCGGG CTCAATGAGG CGCTGCTGGC CAAGGCGGCC AGCGAAAAGC TGTTGCGCAC CAACAAGGTC CGTGCCGACA CCACCGTGGT GGAGGGCGAT GTGGGCTATC CCACCGACAC TGGACTGCTC GCCAAGGCGG TCGGCTCGAT GGCGCGCACC GTGGCGCGGA TCAAAGCCGC GGACGCGGGA TCGGCGCCGC TCGGTGGGTC GTCGGGCCCG CGCGATCGCC TCCAAGCTGC GGTTACGCGG CGCGCAGCAA CGCGATCAGG CGCAGGCCTT CGTGCGCCGG ATCACCGGGG AGCTAGCCGG GATCGCCGAG CAGGCGCTGA CCGAGGCTGC CGCGGTGGTA CG
Details for MytuD.17328.a.A1.PS01036
PURIFICATION DATe: 4/7/2011
CONCENTRATION: 7.41mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 50µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVFRTVGDQ ASLWESVLPE ELRRLPEELA RVDALLDDSA FFCPFVPFFD PRMGRPSIPM ETYLRLMFLK FRYRLGYESL CREVTDSITW RRFCRIPLEG SVPHPTTLMK LTTRCGEDAV AGLNEALLAK AASEKLLRTN KVRADTTVVE GDVGYPTDTG LLAKAVGSMA RTVARIKAAD AGSAPLGGSS GPRDRLQAAV TRRAATRSGA GLRAPDHRGA SRDRRAGADR GCRG
Validated NT Sequence
accaccgcgg cagcctcggt cagcgcctgc tcggcgatcc cggctagctc cccggtgatc cggcgcacga aggcctgcgc ctgatcgcgt tgctgcgcgc cgcgtaaccg cagcttggag gcgatcgcgc gggcccgacg acccaccgag cggcgccgat cccgcgtccg cggctttgat ccgcgccacg gtgcgcgcca tcgagccgac cgccttggcg agcagtccag tgtcggtggg atagcccaca tcgccctcca ccacggtggt gtcggcacgg accttgttgg tgcgcaacag cttttcgctg gccgccttgg ccagcagcgc ctcattgagc ccggccaccg catcctcacc gcagcgcgtg gtcagcttca tcaacgtggt tgggtgcggc accgatccct ccaacggaat acggcagaac cgccgccagg tgatcgaatc ggtgacctcc cgacacagcg actcatagcc caaccggtaa cggaacttca agaacatcaa ccgcaaatag gtctccatcg gtatggacgg ccgacccatc cgcgggtcga agaacggcac aaacgggcag aagaacgccg aatcatcgag cagcgcatcc acccgggcca gctcttcggg cagccgccgc aactcctcgg gcagcacgga ttcccacaac gatgcctgat cgcctacagt acgaaacacc atcgaaccag gaccctgggt ctgagcttcc agggtaccca tatgatggtg atggtgatga gccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVFRTVGDQ ASLWESVLPE ELRRLPEELA RVDALLDDSA FFCPFVPFFD PRMGRPSIPM ETYLRLMFLK FRYRLGYESL CREVTDSITW RRFCRIPLEG SVPHPTTLMK LTTRCGEDAV AGLNEALLAK AASEKLLRTN KVRADTTVVE GDVGYPTDTG LLAKAVGSMA RTVARIKAAD AGSAPLGGSS GPRDRLQAAV TRRAATRSGA GLRAPDHRGA SRDRRAGADR GCRGGT
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGTTTCGT ACTGTAGGCG ATCAGGCATC GTTGTGGGAA TCCGTGCTGC CCGAGGAGTT GCGGCGGCTG CCCGAAGAGC TGGCCCGGGT GGATGCGCTG CTCGATGATT CGGCGTTCTT CTGCCCGTTT GTGCCGTTCT TCGACCCGCG GATGGGTCGG CCGTCCATAC CGATGGAGAC CTATTTGCGG TTGATGTTCT TGAAGTTCCG TTACCGGTTG GGCTATGAGT CGCTGTGTCG GGAGGTCACC GATTCGATCA CCTGGCGGCG GTTCTGCCGT ATTCCGTTGG AGGGATCGGT GCCGCACCCA ACCACGTTGA TGAAGCTGAC CACGCGCTGC GGTGAGGATG CGGTGGCCGG GCTCAATGAG GCGCTGCTGG CCAAGGCGGC CAGCGAAAAG CTGTTGCGCA CCAACAAGGT CCGTGCCGAC ACCACCGTGG TGGAGGGCGA TGTGGGCTAT CCCACCGACA CTGGACTGCT CGCCAAGGCG GTCGGCTCGA TGGCGCGCAC CGTGGCGCGG ATCAAAGCCG CGGACGCGGG ATCGGCGCCG CTCGGTGGGT CGTCGGGCCC GCGCGATCGC CTCCAAGCTG CGGTTACGCG GCGCGCAGCA ACGCGATCAG GCGCAGGCCT TCGTGCGCCG GATCACCGGG GAGCTAGCCG GGATCGCCGA GCAGGCGCTG ACCGAGGCTG CCGCGGTGGT ACGTAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA