MytuD.17324.b

Magnesium chelatase, putative

CENTER ID: MytuD.17324.b
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: diffraction
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:
IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
MytuD.17324.b.A1.GE30723 Full length( MytuD.17324.b ) 1 358
MytuD.17324.b.AE1.GE43172 Full length( MytuD.17324.b ) 1 358
MytuD.17324.b.B1.GE43094 Full length( MytuD.17324.b ) 1 358
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.4115
RefSeq: NP_218209.1
TubercuList: Rv3692
MTB Network Portal: Rv3692
UniProt: I6YGX9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTQSASNPQA PPTQTPGAEL PGYPPQAGGA PTAAPSGPHP HRAEAESARD ALLALRAEVA KAVVGQDGVI SGLVIALLCR GHVLLEGVPG VAKTLIVRAM SAALQLEFKR VQFTPDLMPG DVTGSLVYDA RTAEFVFRPG PVFTNLLLAD EINRTPPKTQ AALLEAMEER QVSVEGEPKP LPNPFIVAAT QNPIEYEGTY QLPEAQLDRF LLKLNVTLPA RDSEIAILDR HAHGFDPRDL SAINPVAGPA ELAAGREAVR HVLVANEVLG YIVDIVGATR SSPALQLGVS PRGATALLGT ARSWAWLSGR DYVTPDDVKA MARPTLRHRV MLRPEAELEG ATPDGVLDGI LASVPVPR
NT Sequence
GTGACACAGT CCGCGTCCAA CCCGCAAGCT CCTCCCACCC AAACCCCTGG CGCTGAATTG CCCGGCTATC CCCCGCAAGC GGGTGGTGCC CCTACAGCGG CCCCTTCCGG GCCGCATCCT CACCGGGCTG AAGCAGAATC GGCACGTGAT GCATTGCTGG CATTACGCGC CGAGGTCGCC AAGGCCGTCG TCGGACAGGA CGGGGTGATC AGCGGCCTGG TGATCGCTCT GTTGTGCCGT GGGCACGTGC TCCTGGAAGG TGTTCCAGGA GTGGCGAAGA CGCTGATTGT CCGCGCTATG TCCGCCGCTT TGCAACTGGA GTTCAAGCGG GTGCAGTTCA CCCCTGACCT GATGCCAGGC GACGTCACCG GTTCACTGGT CTACGATGCC CGCACCGCCG AGTTCGTGTT CCGGCCGGGC CCGGTGTTCA CCAATTTGCT GCTGGCCGAT GAGATCAACC GCACCCCACC CAAGACGCAG GCCGCGCTGC TCGAGGCGAT GGAAGAGCGT CAAGTCAGTG TGGAGGGTGA GCCTAAGCCG CTGCCCAACC CGTTCATCGT CGCCGCGACG CAGAACCCGA TCGAATACGA GGGCACCTAT CAGTTGCCCG AAGCCCAACT GGATCGTTTC CTGCTGAAAC TGAATGTGAC ACTGCCGGCA CGCGATTCCG AGATCGCCAT CCTTGACCGG CACGCGCACG GGTTCGACCC GCGCGATCTA TCCGCGATCA ATCCGGTGGC CGGGCCGGCC GAGCTGGCGG CTGGCCGCGA GGCGGTGCGC CACGTGCTGG TCGCTAATGA GGTGCTGGGC TACATCGTCG ACATCGTCGG GGCCACCCGC TCCTCGCCCG CACTACAGCT CGGTGTGTCG CCGCGTGGGG CAACCGCCCT GCTGGGCACC GCCCGGTCCT GGGCGTGGCT GTCCGGGCGC GATTACGTCA CCCCCGACGA CGTGAAGGCG ATGGCCCGAC CGACGCTACG CCACCGGGTG ATGCTACGCC CGGAAGCCGA GCTGGAAGGC GCCACACCCG ACGGCGTTCT CGACGGAATT CTGGCCTCGG TTCCGGTGCC CCGC
Details for MytuD.17324.b.A1.GE30723
HARVESTED ON: 11/9/2010
SEQUENCED ON: 11/15/2010
EXPECTED MW: 40kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Validated AA Sequence
MVTQSASNPQ APPTQTPGAE LPGYPPQAGG APTAAPSGPH PHRAEAESAR DALLALRAEV AKAVVGQDGV ISGLVIALLC RGHVLLEGVP GVAKTLIVRA MSAALQLEFK RVQFTPDLMP GDVTGSLVYD ARTAEFVFRP GPVFTNLLLA DEINRTPPKT QAALLEAMEE RQVSVEGEPK PLPNPFIVAA TQNPIEYEGT YQLPEAQLDR FLLKLNVTLP ARDSEIAILD RHAHGFDPRD LSAINPVAGP AELAAGREAV RHVLVANEVL GYIVDIVGAT RSSPALQLGV SPRGATALLG TARSWAWLSG RDYVTPDDVK AMARPTLRHR VMLRPEAELE GATPDGVLDG ILASVPVP
Validated NT Sequence
cggggcaccg gaaccgaggc cagaattccg tcgagaacgc cgtcgggtgt ggcgccttcc agctcggctt ccgggcgtag catcacccgg tggcgtagcg tcggtcgggc catcgccttc acgtcgtcgg gggtgacgta atcgcgcccg gacagccacg cccaggaccg ggcggtgccc agcagggcgg ttgccccacg cggcgacaca ccgagctgta gtgcgggcga ggagcgggtg gccccgacga tgtcgacgat gtagcccagc acctcattag cgaccagcac gtggcgcacc gcctcgcggc cagccgccag ctcggccggc ccggccaccg gattgatcgc ggatagatcg cgcgggtcga acccgtgcgc gtgccggtca aggatggcga tctcggaatc gcgtgccggc agtgtcacat tcagtttcag caggaaacga tccagttggg cttcgggcaa ctgataggtg ccctcgtatt cgatcgggtt ctgcgtcgcg gcgacgatga acgggttggg cagcggctta ggctcaccct ccacactgac ttgacgctct tccatcgcct cgagcagcgc ggcctgcgtc ttgggtgggg tgcggttgat ctcatcggcc agcagcaaat tggtgaacac cgggcccggc cggaacacga actcggcggt gcgggcatcg tagaccagtg aaccggtgac gtcgcctggc atcaggtcag gggtgaactg cacccgcttg aactccagtt gcaaagcggc ggacatagcg cggacaatca gcgtcttcgc cactcctgga acaccttcca ggagcacgtg cccacggcac aacagagcga tcaccaggcc gctgatcacc ccgtcctgtc cgacgacggc cttggcgacc tcggcgcgta atgccagcaa tgcatcacgt gccgattctg cttcagcccg gtgaggatgc ggcccggaag gggccgctgt aggggcacca cccgcttgcg ggggatagcc gggcaattca gcgccagggg tttgggtggg aggagcttgc gggttggacg cggactgtgt caccatcgaa ccaggaccct gggtctgagc ttccag
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVTQSASNP QAPPTQTPGA ELPGYPPQAG GAPTAAPSGP HPHRAEAESA RDALLALRAE VAKAVVGQDG VISGLVIALL CRGHVLLEGV PGVAKTLIVR AMSAALQLEF KRVQFTPDLM PGDVTGSLVY DARTAEFVFR PGPVFTNLLL ADEINRTPPK TQAALLEAME ERQVSVEGEP KPLPNPFIVA ATQNPIEYEG TYQLPEAQLD RFLLKLNVTL PARDSEIAIL DRHAHGFDPR DLSAINPVAG PAELAAGREA VRHVLVANEV LGYIVDIVGA TRSSPALQLG VSPRGATALL GTARSWAWLS GRDYVTPDDV KAMARPTLRH RVMLRPEAEL EGATPDGVLD GILASVPVPR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgacacag tccgcgtcca acccgcaagc tcctcccacc caaacccctg gcgctgaatt gcccggctat cccccgcaag cgggtggtgc ccctacagcg gccccttccg ggccgcatcc tcaccgggct gaagcagaat cggcacgtga tgcattgctg gcattacgcg ccgaggtcgc caaggccgtc gtcggacagg acggggtgat cagcggcctg gtgatcgctc tgttgtgccg tgggcacgtg ctcctggaag gtgttccagg agtggcgaag acgctgattg tccgcgctat gtccgccgct ttgcaactgg agttcaagcg ggtgcagttc acccctgacc tgatgccagg cgacgtcacc ggttcactgg tctacgatgc ccgcaccgcc gagttcgtgt tccggccggg cccggtgttc accaatttgc tgctggccga tgagatcaac cgcaccccac ccaagacgca ggccgcgctg ctcgaggcga tggaagagcg tcaagtcagt gtggagggtg agcctaagcc gctgcccaac ccgttcatcg tcgccgcgac gcagaacccg atcgaatacg agggcaccta tcagttgccc gaagcccaac tggatcgttt cctgctgaaa ctgaatgtga cactgccggc acgcgattcc gagatcgcca tccttgaccg gcacgcgcac gggttcgacc cgcgcgatct atccgcgatc aatccggtgg ccgggccggc cgagctggcg gctggccgcg aggcggtgcg ccacgtgctg gtcgctaatg aggtgctggg ctacatcgtc gacatcgtcg gggccacccg ctcctcgccc gcactacagc tcggtgtgtc gccgcgtggg gcaaccgccc tgctgggcac cgcccggtcc tgggcgtggc tgtccgggcg cgattacgtc acccccgacg acgtgaaggc gatggcccga ccgacgctac gccaccgggt gatgctacgc ccggaagccg agctggaagg cgccacaccc gacggcgttc tcgacggaat tctggcctcg gttccggtgc cccgctaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for MytuD.17324.b.AE1.GE43172
HARVESTED ON: 5/17/2019
SEQUENCED ON: 5/24/2019
EXPECTED MW: 39kDa
OBSERVED MW: 39kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 94
PERCENT COVERAGE: 65
Validated AA Sequence
MPAPPSSCXX XGPVFTNLLL ADEINRTPPK TQAALLEAME ERQVSVEGEP KPLPNPFIVA ATXXPIEYEG TYQLPEAQLD RXLLKLNVTL PARDSEIAIL DRHAHGFDPR DLSAINPVAG PAELAAGREA VRHVLVANEV LGYIVDIVGA TRSSPALQLG VSPRGATALL GTARSWAWLS GRDYVTPDDV KAMARPTXRH RVMLRPEAEL EGATPDGVLD GILASVPVPR AGHHHHHH
Validated NT Sequence
tccgttaatg atgatgatgg tggtgcccag cgcggggcac cggaaccgag gccagaattc cgtcgagaac gccgtcgggt gtggcncctt ccagctcggc ttccgggcgt agcatcaccc ggtggcgtnn ngtcggtcgg gccatcgcct tcacgtcgtc gggggtgacg taatcgcgcc cggacagcca cgcccaggac cgggcggtgc ccagcagggc ggttgcccca cgcggcgaca caccgagctg tagtgcgggc gaggagcggg tggccccgac gatgtcgacg atgtagccca gcacctcatt agcgaccagc acgtggcgca ccgcctcgcg gccagccgcc agctcggccg gcccggccac cggattgatc gcggatagat cgcgcgggtc gaacccgtgc gcgtgccggt caaggatggc gatctcggaa tcgcgtgccg gcagtgtcac attcagtttc agcagnaaac gatccagttg ggcttcgggc aactgatagg tgccctcgta ttcgatcggg tnnnncgtcg cggcgacgat gaacgggttg ggcagcggct tnggctcacc ctccacactg acttgacgct cttccatcgc ctcgagcagc gcggcctgcg tcttgggtgg ggtgcggttg atctcatcgg ccagcagcaa attggtgaac accgggcccg ncnnnnaaca cgaactcggc ggtgcgggca tcgtagacca gtgaaccggt gacgtcgcct ggcatcaggt caggggtgaa ctgcacccgc ttgaactcca gttgcaaagc ggcggacata gcgcggacaa tcagcgtctt cgccactcct ggaacacctt ccaggagcac gtgcccacgg cacaacagag cgatcaccag gccgctgatc accccgtcct gtccgacgac gnncttggcg acctcggcgc gtaatgccag caatgcatca cgtgccgatt ntgcttcagc ccggtgagga tgcggcccgg aaggggccgc tgtaggggca ccacccgctt gcgggggata gccgggcaat tcagcgccag gggtttgggt gggaggagct tgcgggttgg acgcggactg tgtcaccatg ggagaga
Expected Protein Sequence
MTQSASNPQA PPTQTPGAEL PGYPPQAGGA PTAAPSGPHP HRAEAESARD ALLALRAEVA KAVVGQDGVI SGLVIALLCR GHVLLEGVPG VAKTLIVRAM SAALQLEFKR VQFTPDLMPG DVTGSLVYDA RTAEFVFRPG PVFTNLLLAD EINRTPPKTQ AALLEAMEER QVSVEGEPKP LPNPFIVAAT QNPIEYEGTY QLPEAQLDRF LLKLNVTLPA RDSEIAILDR HAHGFDPRDL SAINPVAGPA ELAAGREAVR HVLVANEVLG YIVDIVGATR SSPALQLGVS PRGATALLGT ARSWAWLSGR DYVTPDDVKA MARPTLRHRV MLRPEAELEG ATPDGVLDGI LASVPVPRGH HHHHH
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atttgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat cgatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt taagaaggag tctctcccgt gacacagtcc gcgtccaacc cgcaagctcc tcccacccaa acccctggcg ctgaattgcc cggctatccc ccgcaagcgg gtggtgcccc tacagcggcc ccttccgggc cgcatcctca ccgggctgaa gcagaatcgg cacgtgatgc attgctggca ttacgcgccg aggtcgccaa ggccgtcgtc ggacaggacg gggtgatcag cggcctggtg atcgctctgt tgtgccgtgg gcacgtgctc ctggaaggtg ttccaggagt ggcgaagacg ctgattgtcc gcgctatgtc cgccgctttg caactggagt tcaagcgggt gcagttcacc cctgacctga tgccaggcga cgtcaccggt tcactggtct acgatgcccg caccgccgag ttcgtgttcc ggccgggccc ggtgttcacc aatttgctgc tggccgatga gatcaaccgc accccaccca agacgcaggc cgcgctgctc gaggcgatgg aagagcgtca agtcagtgtg gagggtgagc ctaagccgct gcccaacccg ttcatcgtcg ccgcgacgca gaacccgatc gaatacgagg gcacctatca gttgcccgaa gcccaactgg atcgtttcct gctgaaactg aatgtgacac tgccggcacg cgattccgag atcgccatcc ttgaccggca cgcgcacggg ttcgacccgc gcgatctatc cgcgatcaat ccggtggccg ggccggccga gctggcggct ggccgcgagg cggtgcgcca cgtgctggtc gctaatgagg tgctgggcta catcgtcgac atcgtcgggg ccacccgctc ctcgcccgca ctacagctcg gtgtgtcgcc gcgtggggca accgccctgc tgggcaccgc ccggtcctgg gcgtggctgt ccgggcgcga ttacgtcacc cccgacgacg tgaaggcgat ggcccgaccg acgctacgcc accgggtgat gctacgcccg gaagccgagc tggaaggcgc cacacccgac ggcgttctcg acggaattct ggcctcggtt ccggtgcccc gcgggcacca ccatcatcat cattaacgga tccgaattcg agctccgtcg acaagcttgc ggccgcactc gagcaccacc accaccacca ctgagatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat
Details for MytuD.17324.b.B1.GE43094
HARVESTED ON: 4/19/2019
SEQUENCED ON: 4/23/2019
EXPECTED MW: 39kDa
OBSERVED MW: 39kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMV TQSASNPQAP PTQTPGAELP GYPPQAGGAP TAAPSGPHPH RAEAESARDA LLALRAEVAK AVVGQDGVIS GLVIALLCRG HVLLEGVPGV AKTLIVRAMS AALQLEFKRV QFTPDLMPGD VTGSLVYDAR TAEFVFRPGP VFTNLLLADE INRTPPKTQA ALLEAMEERQ VSVEGEPKPL PNPFIVAATQ NPIEYEGTYQ LPEAQLDRFL LKLNVTLPAR DSEIAILDRH AHGFDPRDLS AINPVAGPAE LAAGREAVRH VLVANEVLGY IVDIVGATRS SPALQLGVSP RGATALLGTA RSWAWLSGRD YVTPDDVKAM ARPTLRHRVM LRPEAELEGA TPDGVLD
Validated NT Sequence
cgtcgagaac gccgtcgggt gtggcgcctt ccagctcggc ttccgggcgt agcatcaccc ggtggcgtag cgtcggtcgg gccatcgcct tcacgtcgtc gggggtgacg taatcgcgcc cggacagcca cgcccaggac cgggcggtgc ccagcagggc ggttgcccca cgcggcgaca caccgagctg tagtgcgggc gaggagcggg tggccccgac gatgtcgacg atgtagccca gcacctcatt agcgaccagc acgtggcgca ccgcctcgcg gccagccgcc agctcggccg gcccggccac cggattgatc gcggatagat cgcgcgggtc gaacccgtgc gcgtgccggt caaggatggc gatctcggaa tcgcgtgccg gcagtgtcac attcagtttc agcaggaaac gatccagttg ggcttcgggc aactgatagg tgccctcgta ttcgatcggg ttctgcgtcg cggcgacgat gaacgggttg ggcagcggct taggctcacc ctccacactg acttgacgct cttccatcgc ctcgagcagc gcggcctgcg tcttgggtgg ggtgcggttg atctcatcgg ccagcagcaa attggtgaac accgggcccg gccggaacac gaactcggcg gtgcgggcat cgtagaccag tgaaccggtg acgtcgcctg gcatcaggtc aggggtgaac tgcacccgct tgaactccag ttgcaaagcg gcggacatag cgcggacaat cagcgtcttc gccactcctg gaacaccttc caggagcacg tgcccacggc acaacagagc gatcaccagg ccgctgatca ccccgtcctg tccgacgacg gccttggcga cctcggcgcg taatgccagc aatgcatcac gtgccgattc tgcttcagcc cggtgaggat gcggcccgga aggggccgct gtaggggcac cacccgcttg cgggggatag ccgggcaatt cagcgccagg ggtttgggtg ggaggagctt gcgggttgga cgcggactgt gtcaccatat ggtggtggtg gtggtgagcc at
Expected Protein Sequence
MAHHHHHHMT QSASNPQAPP TQTPGAELPG YPPQAGGAPT AAPSGPHPHR AEAESARDAL LALRAEVAKA VVGQDGVISG LVIALLCRGH VLLEGVPGVA KTLIVRAMSA ALQLEFKRVQ FTPDLMPGDV TGSLVYDART AEFVFRPGPV FTNLLLADEI NRTPPKTQAA LLEAMEERQV SVEGEPKPLP NPFIVAATQN PIEYEGTYQL PEAQLDRFLL KLNVTLPARD SEIAILDRHA HGFDPRDLSA INPVAGPAEL AAGREAVRHV LVANEVLGYI VDIVGATRSS PALQLGVSPR GATALLGTAR SWAWLSGRDY VTPDDVKAMA RPTLRHRVML RPEAELEGAT PDGVLDGILA SVPVPR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgacac agtccgcgtc caacccgcaa gctcctccca cccaaacccc tggcgctgaa ttgcccggct atcccccgca agcgggtggt gcccctacag cggccccttc cgggccgcat cctcaccggg ctgaagcaga atcggcacgt gatgcattgc tggcattacg cgccgaggtc gccaaggccg tcgtcggaca ggacggggtg atcagcggcc tggtgatcgc tctgttgtgc cgtgggcacg tgctcctgga aggtgttcca ggagtggcga agacgctgat tgtccgcgct atgtccgccg ctttgcaact ggagttcaag cgggtgcagt tcacccctga cctgatgcca ggcgacgtca ccggttcact ggtctacgat gcccgcaccg ccgagttcgt gttccggccg ggcccggtgt tcaccaattt gctgctggcc gatgagatca accgcacccc acccaagacg caggccgcgc tgctcgaggc gatggaagag cgtcaagtca gtgtggaggg tgagcctaag ccgctgccca acccgttcat cgtcgccgcg acgcagaacc cgatcgaata cgagggcacc tatcagttgc ccgaagccca actggatcgt ttcctgctga aactgaatgt gacactgccg gcacgcgatt ccgagatcgc catccttgac cggcacgcgc acgggttcga cccgcgcgat ctatccgcga tcaatccggt ggccgggccg gccgagctgg cggctggccg cgaggcggtg cgccacgtgc tggtcgctaa tgaggtgctg ggctacatcg tcgacatcgt cggggccacc cgctcctcgc ccgcactaca gctcggtgtg tcgccgcgtg gggcaaccgc cctgctgggc accgcccggt cctgggcgtg gctgtccggg cgcgattacg tcacccccga cgacgtgaag gcgatggccc gaccgacgct acgccaccgg gtgatgctac gcccggaagc cgagctggaa ggcgccacac ccgacggcgt tctcgacgga attctggcct cggttccggt gccccgctga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at