MytuD.17310.a

CONSERVED HYPOTHETICAL PROTEIN TB31.7

CENTER ID: MytuD.17310.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.17310.a.B1.GE39933 full length 1 294
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.2264
RefSeq: NP_216542
TubercuList: Rv2026c
MTB Network Portal: Rv2026c
UniProt: P9WFD1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSAATAKYGI LVGVDGSAQS NAAVAWAARE AVMRQLPITL LHIVAPVVVG WPVGQLYANM TEWQKDNAQQ VIEQAREALT NSLGESKPPQ VHTELVFSNV VPTLIDASQQ AWLMVVGSQG MGALGRLLLG SISTALLHHA RCPVAIIHSG NGATPDSDAP VLVGIDGSPA SEAATALAFD EASRRRVDLV ALHAWTDLGM FPVLGMDWRE REKREAEVLA ERLAGWQEQY PDVRVHRSLV CDKPARWLLE HSEQAQLVVV GSHGRGGFSG MLLGSVSSAV AHSVRIPVIV VRPS
NT Sequence
atgtctgctg cgacagcgaa atacggaata ctcgttggcg tcgacggatc agcgcaatcc aacgcggcgg tagcctgggc cgcccgtgag gccgtcatgc gccagctgcc catcaccctg ctgcacattg ttgcccctgt ggttgtcggc tggccggtgg gccagctgta cgcgaatatg actgagtggc aaaaggacaa cgcacagcag gtcattgagc aggcccgcga ggcgctcacc aacagcttgg gtgagtccaa accgccgcag gtgcataccg agttggtctt ttccaatgtt gtgccgacgc ttatcgatgc atcccaacag gcttggttga tggtcgtcgg cagccagggg atgggcgcat tgggtcgact gctgctcggc tcgatcagca ccgcgttgct ccaccatgcg cggtgtccag tggccatcat tcattccggt aatggcgcga ctcccgactc cgatgcaccg gtgttggtgg gcatcgacgg atcgccagcc tctgaggcgg caacggccct agccttcgac gaagcctccc gccgaagggt ggacctggtg gcgttgcacg cctggaccga tctggggatg tttccggttc tggggatgga ttggcgagag cgcgagaaga gagaggctga ggtcctcgct gaacgcctcg ccggctggca agagcagtat ccggatgtcc gcgtgcaccg atcgctggtg tgcgacaagc ccgctcggtg gctgctcgag cattctgagc aagctcagct ggtggtggtc ggcagccatg ggcgcggggg gttttccggc atgctgttgg gttcggtgag ttccgcggta gcccattcgg tgagaatccc ggtgatcgtc gtccgtccca gctag
Details for MytuD.17310.a.B1.GE39933
HARVESTED ON: 2/26/2016
SEQUENCED ON: 3/1/2016
EXPECTED MW: 32kDa
OBSERVED MW: 32kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMS AATAKYGILV GVDGSAQSNA AVAWAAREAV MRQLPITLLH IVAPVVVGWP VGQLYANMTE WQKDNAQQVI EQAREALTNS LGESKPPQVH TELVFSNVVP TLIDASQQAW LMVVGSQGMG ALGRLLLGSI STALLHHARC PVAIIHSGNG ATPDSDAPVL VGIDGSPASE AATALAFDEA SRRRVDLVAL HAWTDLGMFP VLGMDWRERE KREAEVLAER LAGWQEQYPD VRVHRSLVCD KPARWLLEHS EQAQLVVVGS HGRGGFSGML LGSVSSAVAH SVRXXXDRRP SQLSE
Validated NT Sequence
atcttactca cttagctggg acggacgacg atcacnnnnn nttctcaccg aatgggctac cgcggaactc accgaaccca acagcatgcc ggaaaacccc ccgcgcccat ggctgccgac caccaccagc tgagcttgct cagaatgctc gagcagccac cgagcgggct tgtcgcacac cagcgatcgg tgcacgcgga catccggata ctgctcttgc cagccggcga ggcgttcagc gaggacctca gcctctctct tctcgcgctc tcgccaatcc atccccagaa ccggaaacat ccccagatcg gtccaggcgt gcaacgccac caggtccacc cttcggcggg aggcttcgtc gaaggctagg gccgttgccg cctcagaggc tggcgatccg tcgatgccca ccaacaccgg tgcatcggag tcgggagtcg cgccattacc ggaatgaatg atggccactg gacaccgcgc atggtggagc aacgcggtgc tgatcgagcc gagcagcagt cgacccaatg cgcccatccc ctggctgccg acgaccatca accaagcctg ttgggatgca tcgataagcg tcggcacaac attggaaaag accaactcgg tatgcacctg cggcggtttg gactcaccca agctgttggt gagcgcctcg cgggcctgct caatgacctg ctgtgcgttg tccttttgcc actcagtcat attcgcgtac agctggccca ccggccagcc gacaaccaca ggggcaacaa tgtgcagcag ggtgatgggc agctggcgca tgacggcctc acgggcggcc caggctaccg ccgcgttgga ttgcgctgat ccgtcgacgc caacgagtat tccgtatttc gctgtcgcag cagacatatg gtggtggtgg tggtgagcca t
Expected Protein Sequence
MAHHHHHHMS AATAKYGILV GVDGSAQSNA AVAWAAREAV MRQLPITLLH IVAPVVVGWP VGQLYANMTE WQKDNAQQVI EQAREALTNS LGESKPPQVH TELVFSNVVP TLIDASQQAW LMVVGSQGMG ALGRLLLGSI STALLHHARC PVAIIHSGNG ATPDSDAPVL VGIDGSPASE AATALAFDEA SRRRVDLVAL HAWTDLGMFP VLGMDWRERE KREAEVLAER LAGWQEQYPD VRVHRSLVCD KPARWLLEHS EQAQLVVVGS HGRGGFSGML LGSVSSAVAH SVRIPVIVVR PS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtctg ctgcgacagc gaaatacgga atactcgttg gcgtcgacgg atcagcgcaa tccaacgcgg cggtagcctg ggccgcccgt gaggccgtca tgcgccagct gcccatcacc ctgctgcaca ttgttgcccc tgtggttgtc ggctggccgg tgggccagct gtacgcgaat atgactgagt ggcaaaagga caacgcacag caggtcattg agcaggcccg cgaggcgctc accaacagct tgggtgagtc caaaccgccg caggtgcata ccgagttggt cttttccaat gttgtgccga cgcttatcga tgcatcccaa caggcttggt tgatggtcgt cggcagccag gggatgggcg cattgggtcg actgctgctc ggctcgatca gcaccgcgtt gctccaccat gcgcggtgtc cagtggccat cattcattcc ggtaatggcg cgactcccga ctccgatgca ccggtgttgg tgggcatcga cggatcgcca gcctctgagg cggcaacggc cctagccttc gacgaagcct cccgccgaag ggtggacctg gtggcgttgc acgcctggac cgatctgggg atgtttccgg ttctggggat ggattggcga gagcgcgaga agagagaggc tgaggtcctc gctgaacgcc tcgccggctg gcaagagcag tatccggatg tccgcgtgca ccgatcgctg gtgtgcgaca agcccgctcg gtggctgctc gagcattctg agcaagctca gctggtggtg gtcggcagcc atgggcgcgg ggggttttcc ggcatgctgt tgggttcggt gagttccgcg gtagcccatt cggtgagaat cccggtgatc gtcgtccgtc ccagctgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat