MytuD.17291.b

Luciferase-related protein

CENTER ID: MytuD.17291.b
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.17291.b.A1.GE30715 Full length( MytuD.17291.b ) 1 369
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.2164
RefSeq: NP_216452.1
TubercuList: Rv1936
MTB Network Portal: Rv1936
UniProt: P95278

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEIGIFLMPA HPPERTLYDA TRWDLDVIEL ADQLGYVEAW VGEHFTVPWE PICAPDLLLA QALLRTQQIK LAPGAHLLPY HHPVELAHRV AYFDHLAQGR FMLGVGASGI PGDWALYDVD GKNGEHREMT REALEIMLRI WTEDEPWEHR GKYWNANGIA PMFEGLMRRH IKPYQKPHPP IGVTGFSAGS ETLKLAGERG YIPMSLDLNT EYVATHWDAV EEGALRSGRT PDRRDWRLVR EVLVAETDEQ AFRYAVDGTM GRAMREYVLP TFRMFGMTKF YKHNPSVPDD EVTPEYLAEN TFVVGSVQTV VDKLEATYDQ VGGFGHLLIL GFDYSDNPGP WKESLRLLAH EVMPRLNARL ATKPATAVV
NT Sequence
ATGGAGATCG GAATATTCCT CATGCCGGCC CATCCACCGG AGCGCACCCT CTACGACGCC ACCCGGTGGG ATCTGGACGT CATCGAGCTG GCCGATCAAC TCGGCTACGT GGAGGCCTGG GTCGGCGAAC ACTTCACCGT GCCGTGGGAG CCGATCTGCG CCCCCGATCT GCTGTTGGCG CAGGCGCTGC TGCGCACCCA ACAGATCAAG CTCGCCCCGG GTGCGCACTT GTTGCCCTAC CATCATCCGG TCGAGTTGGC CCACCGGGTG GCCTATTTCG ACCACCTCGC CCAGGGTCGG TTCATGCTCG GCGTGGGCGC CAGCGGCATC CCGGGTGACT GGGCGCTGTA TGACGTGGAC GGCAAGAACG GCGAGCATCG CGAAATGACC CGGGAAGCGC TGGAGATCAT GCTGCGCATC TGGACCGAGG ACGAGCCCTG GGAGCATCGC GGAAAGTACT GGAACGCCAA CGGAATCGCG CCGATGTTCG AGGGTCTGAT GAGGCGCCAC ATCAAGCCGT ACCAGAAGCC CCACCCGCCC ATCGGCGTCA CCGGGTTCAG CGCCGGCTCG GAGACCCTCA AGCTCGCCGG CGAACGGGGT TACATCCCCA TGAGTCTGGA CCTCAACACC GAATACGTCG CCACCCACTG GGACGCGGTG GAGGAAGGCG CGCTGCGCAG CGGGCGAACC CCGGATCGCC GCGATTGGCG GCTGGTGCGG GAGGTGCTGG TGGCCGAGAC CGATGAGCAG GCGTTCCGGT ATGCCGTGGA CGGCACGATG GGACGCGCCA TGCGTGAGTA TGTGCTGCCG ACGTTTCGGA TGTTCGGCAT GACCAAGTTC TACAAACACA ATCCGTCGGT GCCCGACGAC GAGGTGACAC CGGAGTATCT CGCCGAGAAC ACCTTCGTGG TCGGCTCGGT GCAGACCGTG GTCGACAAGC TCGAGGCCAC CTACGACCAG GTCGGCGGGT TCGGCCACCT GCTGATCCTC GGGTTCGACT ACAGCGATAA CCCGGGCCCG TGGAAGGAGT CGTTGCGGCT GCTGGCCCAC GAGGTCATGC CCAGACTCAA CGCCCGCCTC GCCACCAAGC CCGCCACCGC GGTGGTG
Details for MytuD.17291.b.A1.GE30715
HARVESTED ON: 11/9/2010
SEQUENCED ON: 11/15/2010
EXPECTED MW: 44kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 20
PERCENT COVERAGE: 46
Validated AA Sequence
MRASPADGLA ITGLSWKGSR GGSVREVRGG TCPLSSGRGK RCGSAITVGR WMVPATRCSP TLPRCSGWTL RWPRISRSCC RWIPRTCGHT SIRRAPARTR SPQGALSDYK KSADNKQHEQ VLQPSFSRIR LLTKPERKLS WLLPPLSITS XXXAPPXHRX XXVXRXXGDP QXRRRTGLHX P
Validated NT Sequence
attttgttta ctttangaag gagatatacc atggctcatc accatcacna tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtga cgcgagtggg tgtgatttcg gacgagttct gggccgtggt cgagccgttg atgccgtcgc atgagggcaa gcccggcaga cggtttagcg atcaccggct tatcctggaa gggatcgcgt ggcggttccg tacgggaagt ccgtggcggg acctgcccgc tgagttcggg ccgtggcaaa cggtgtggaa gcgccatcac cgttggtcgc tggatggtac ctgcgacgag gtgttcgccc acgttgccgc ggtgttcggg gtggacgctg aggtggccga ggatatcgag aagctgctgt cggtggattc cacgaacgtg cgggcacacc agcattcggc gggcgcctgc tcggacacgc tcgccacagg gggcactgtc ggattacaag aaatccgccg ataataaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag cataactagc aanncnnang ccccaccnnc ccatcggcnt cnccnggttc ancgncngnt cggagaccct caanctcgcc ggcgaacggg gttacatncc ccatgagtct ggacctcaac nccnaanncn tcgccnccca ctgggncgcn gtggangaag gngcgctgcn nannnngcna acnccnnatc gccncnattg nntgnntnnn ngcggganng ngctggtggn cnagaccnat nannnngcnn ttnnngtntn cnnngnannn nacnntnggc ncgnccatgn gtgagtatnt ngcngncnnn cntttcggga nnaanttnta nnaanacaat nngncnnngn nnnnnnnaca anntgannng gnagtatntn nnncnnanan nnncnnt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMEIGIFLMP AHPPERTLYD ATRWDLDVIE LADQLGYVEA WVGEHFTVPW EPICAPDLLL AQALLRTQQI KLAPGAHLLP YHHPVELAHR VAYFDHLAQG RFMLGVGASG IPGDWALYDV DGKNGEHREM TREALEIMLR IWTEDEPWEH RGKYWNANGI APMFEGLMRR HIKPYQKPHP PIGVTGFSAG SETLKLAGER GYIPMSLDLN TEYVATHWDA VEEGALRSGR TPDRRDWRLV REVLVAETDE QAFRYAVDGT MGRAMREYVL PTFRMFGMTK FYKHNPSVPD DEVTPEYLAE NTFVVGSVQT VVDKLEATYD QVGGFGHLLI LGFDYSDNPG PWKESLRLLA HEVMPRLNAR LATKPATAVV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggagatcgga atattcctca tgccggccca tccaccggag cgcaccctct acgacgccac ccggtgggat ctggacgtca tcgagctggc cgatcaactc ggctacgtgg aggcctgggt cggcgaacac ttcaccgtgc cgtgggagcc gatctgcgcc cccgatctgc tgttggcgca ggcgctgctg cgcacccaac agatcaagct cgccccgggt gcgcacttgt tgccctacca tcatccggtc gagttggccc accgggtggc ctatttcgac cacctcgccc agggtcggtt catgctcggc gtgggcgcca gcggcatccc gggtgactgg gcgctgtatg acgtggacgg caagaacggc gagcatcgcg aaatgacccg ggaagcgctg gagatcatgc tgcgcatctg gaccgaggac gagccctggg agcatcgcgg aaagtactgg aacgccaacg gaatcgcgcc gatgttcgag ggtctgatga ggcgccacat caagccgtac cagaagcccc acccgcccat cggcgtcacc gggttcagcg ccggctcgga gaccctcaag ctcgccggcg aacggggtta catccccatg agtctggacc tcaacaccga atacgtcgcc acccactggg acgcggtgga ggaaggcgcg ctgcgcagcg ggcgaacccc ggatcgccgc gattggcggc tggtgcggga ggtgctggtg gccgagaccg atgagcaggc gttccggtat gccgtggacg gcacgatggg acgcgccatg cgtgagtatg tgctgccgac gtttcggatg ttcggcatga ccaagttcta caaacacaat ccgtcggtgc ccgacgacga ggtgacaccg gagtatctcg ccgagaacac cttcgtggtc ggctcggtgc agaccgtggt cgacaagctc gaggccacct acgaccaggt cggcgggttc ggccacctgc tgatcctcgg gttcgactac agcgataacc cgggcccgtg gaaggagtcg ttgcggctgc tggcccacga ggtcatgccc agactcaacg cccgcctcgc caccaagccc gccaccgcgg tggtgtaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa