MytuD.17291.a

Luciferase-related protein

CENTER ID: MytuD.17291.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.17291.a.A1.GE30714 Full length( MytuD.17291.a ) 1 395
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.4034
RefSeq: NP_218135.1
TubercuList: Rv3618
MTB Network Portal: Rv3618
UniProt: I6X7W8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKAPLRFGVF ITPFHPTGQS PTVALQYDME RVVALDRLGY DEAWFGEHHS GGYELIACPE VFIAAAAERT THIRLGTGVV SLPYHHPLMV ADRWVLLDHL TRGRVMFGTG PGALPSDAYM MGIDPVEQRR MMQESLEAIL ALFRAAPDER IDRHSDWFTL REAQLHIRPY TWPYPEIATA AMISPSGPRL AGALGTSLLS LSMSVPGGYA ALETAWGVVR EQAAKAGRGE PDRADWRVLS IMHLSDSRDQ AIDDCTYGLP DFSRYFGAAG FVPLANTVEG TQSSREFVEQ YAAKGNCCIG TPDDAIAHIE DLLHRSGGFG TLLLLGHDWA PPPATFHSYE LFARAVIPYF KGQLAAPRAS HEWARGKRDQ LIGRAGEAVV KAITEHVAEQ GEAGS
NT Sequence
ATGAAGGCAC CGTTGCGTTT TGGCGTTTTC ATCACGCCAT TCCATCCGAC CGGTCAATCC CCGACCGTGG CGTTGCAATA CGACATGGAG CGCGTCGTTG CGCTGGACCG GCTCGGCTAC GACGAGGCGT GGTTTGGCGA ACACCACTCC GGTGGCTACG AGCTGATCGC TTGCCCGGAG GTGTTTATCG CGGCCGCAGC GGAACGGACC ACCCACATCC GGCTAGGTAC CGGAGTGGTT TCGCTGCCCT ACCATCATCC GCTAATGGTG GCCGACCGTT GGGTGCTGCT GGATCACCTG ACCCGTGGGC GGGTCATGTT CGGCACCGGC CCCGGCGCGC TGCCGTCGGA CGCCTACATG ATGGGCATCG ATCCGGTCGA GCAGCGACGA ATGATGCAGG AGTCCCTCGA GGCGATTCTC GCGCTGTTCC GTGCCGCACC TGACGAGCGA ATCGACCGCC ACTCCGACTG GTTCACCCTG CGTGAAGCGC AATTGCACAT CCGCCCCTAC ACCTGGCCGT ACCCCGAAAT CGCTACCGCA GCCATGATTT CGCCATCGGG TCCGCGACTG GCCGGTGCGC TGGGCACGTC GCTGTTATCA CTGTCGATGT CAGTGCCCGG CGGCTACGCT GCGCTGGAAA CAGCGTGGGG CGTGGTGCGG GAGCAGGCCG CCAAAGCTGG GCGGGGCGAG CCGGATCGCG CCGATTGGCG GGTGTTGAGC ATCATGCACT TGTCGGACAG CCGCGACCAG GCGATCGACG ACTGCACTTA CGGGTTACCC GACTTCTCGA GGTACTTCGG CGCGGCAGGG TTTGTCCCGT TGGCGAACAC CGTGGAAGGC ACCCAGTCGT CTCGGGAATT CGTCGAGCAA TACGCGGCCA AGGGAAATTG CTGCATCGGC ACGCCCGATG ACGCGATCGC CCACATTGAA GACTTGCTGC ACCGGTCGGG TGGCTTCGGA ACGTTGCTAC TGCTCGGCCA CGACTGGGCC CCGCCACCGG CAACCTTTCA CTCCTATGAG CTGTTCGCCC GTGCTGTGAT TCCTTATTTC AAGGGACAAC TCGCGGCGCC GCGGGCGTCG CACGAATGGG CTAGAGGCAA GCGCGACCAA TTGATTGGCC GCGCCGGCGA AGCGGTCGTC AAAGCCATCA CCGAGCACGT CGCCGAACAA GGGGAAGCGG GCAGC
Details for MytuD.17291.a.A1.GE30714
HARVESTED ON: 11/9/2010
SEQUENCED ON: 11/15/2010
EXPECTED MW: 45kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 77
PERCENT COVERAGE: 93
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMKAPLRFGV FITPFHPTGQ SPTVALQYDM ERVVALDRLG YDEAWFGEHH SGGYELIACP EVFIAAAAER TTHIRLGTGV VSLPYHHPLM VADRWVLLDH LTRGRVMFGT GPGALPSDAY MMGIDPVEQR RMMQESLEAI LALFRAAPDE RIDRHSDWFT LREAQLHIRP YTWPYPEIAT AAMISPSGPR LAGALGTSLL SLSMSVPGGY AALETAWGVV REQAAKAGRG EPDRADWRVL SIMHLSDSRD QAIDDCTYGL PDFSRYFGAA XFVPLANTVX XPXRLGNSSS NTRPXXXAAS AXPMTXXXXI EXXXXXXXXX XXXATAXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXX
Validated NT Sequence
attttnttta ctttaagaag gaganatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaagg caccgttgcg ttttggcgtt ttcatcacgc cattccatcc gaccggtcaa tccccgaccg tggcgttgca atacgacatg gagcgcgtcg ttgcgctgga ccggctcggc tacgacgagg cgtggtttgg cgaacaccac tccggtggct acgagctgat cgcttgcccg gaggtgttta tcgcggccgc agcggaacgg accacccaca tccggctagg taccggagtg gtttcgctgc cctaccatca tccgctaatg gtggccgacc gttgggtgct gctggatcac ctgacccgtg ggcgggtcat gttcggcacc ggccccggcg cgctgccgtc ggacgcctac atgatgggca tcgatccggt cgagcagcga cgaatgatgc aggagtccct cgaggcgatt ctcgcgctgt tccgtgccgc acctgacgag cgaatcgacc gccactccga ctggttcacc ctgcgtgaag cgcaattgca catccgcccc tacacctggc cgtaccccga aatcgctacc gcagccatga tttcgccatc gggtccgcga ctggccggtg cgctgggcac gtcgctgtta tcactgtcga tgtcagtgcc cggcggctac gctgcgctgg aaacagcgtg gggcgtggtg cgggagcagg ccgccaaagc tgggcggggc gagccggatc gcgccgattg gcgggtgttg agcatcatgc acttgtcgga cagccgcgac caggcgatcg acgactgcac ttacgggtta cccgacttct cnaggtactt cggcgcggcn nnntttgtcc cgttggcgaa caccgtgnna anacccannc gtctcgggaa ttcntcnagc aatacncggc ccannnnaan tgctgcatcg gcacnnccga tgacnnnnat nncccncatt gaanannnnn tgcancngnt cnggnngntn nnnaannttg ctactgctcn nnnnnnacng nnccnnnnnc nnnnnnnttn nnnnnnntnn ncnnnnnccc nnncnnnnnt nnnnnnnann nnnnnnnnnc nngnnnnnnn nnnnnngnnn tannnnnnnn nnnnnnnnnn nnnnnnnnnc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKAPLRFGV FITPFHPTGQ SPTVALQYDM ERVVALDRLG YDEAWFGEHH SGGYELIACP EVFIAAAAER TTHIRLGTGV VSLPYHHPLM VADRWVLLDH LTRGRVMFGT GPGALPSDAY MMGIDPVEQR RMMQESLEAI LALFRAAPDE RIDRHSDWFT LREAQLHIRP YTWPYPEIAT AAMISPSGPR LAGALGTSLL SLSMSVPGGY AALETAWGVV REQAAKAGRG EPDRADWRVL SIMHLSDSRD QAIDDCTYGL PDFSRYFGAA GFVPLANTVE GTQSSREFVE QYAAKGNCCI GTPDDAIAHI EDLLHRSGGF GTLLLLGHDW APPPATFHSY ELFARAVIPY FKGQLAAPRA SHEWARGKRD QLIGRAGEAV VKAITEHVAE QGEAGS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaaggcaccg ttgcgttttg gcgttttcat cacgccattc catccgaccg gtcaatcccc gaccgtggcg ttgcaatacg acatggagcg cgtcgttgcg ctggaccggc tcggctacga cgaggcgtgg tttggcgaac accactccgg tggctacgag ctgatcgctt gcccggaggt gtttatcgcg gccgcagcgg aacggaccac ccacatccgg ctaggtaccg gagtggtttc gctgccctac catcatccgc taatggtggc cgaccgttgg gtgctgctgg atcacctgac ccgtgggcgg gtcatgttcg gcaccggccc cggcgcgctg ccgtcggacg cctacatgat gggcatcgat ccggtcgagc agcgacgaat gatgcaggag tccctcgagg cgattctcgc gctgttccgt gccgcacctg acgagcgaat cgaccgccac tccgactggt tcaccctgcg tgaagcgcaa ttgcacatcc gcccctacac ctggccgtac cccgaaatcg ctaccgcagc catgatttcg ccatcgggtc cgcgactggc cggtgcgctg ggcacgtcgc tgttatcact gtcgatgtca gtgcccggcg gctacgctgc gctggaaaca gcgtggggcg tggtgcggga gcaggccgcc aaagctgggc ggggcgagcc ggatcgcgcc gattggcggg tgttgagcat catgcacttg tcggacagcc gcgaccaggc gatcgacgac tgcacttacg ggttacccga cttctcgagg tacttcggcg cggcagggtt tgtcccgttg gcgaacaccg tggaaggcac ccagtcgtct cgggaattcg tcgagcaata cgcggccaag ggaaattgct gcatcggcac gcccgatgac gcgatcgccc acattgaaga cttgctgcac cggtcgggtg gcttcggaac gttgctactg ctcggccacg actgggcccc gccaccggca acctttcact cctatgagct gttcgcccgt gctgtgattc cttatttcaa gggacaactc gcggcgccgc gggcgtcgca cgaatgggct agaggcaagc gcgaccaatt gattggccgc gccggcgaag cggtcgtcaa agccatcacc gagcacgtcg ccgaacaagg ggaagcgggc agctaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa