MytuD.17287.a

Probable thioesterase tesA

CENTER ID: MytuD.17287.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.17287.a.A1.GE30713 Full length( MytuD.17287.a ) 1 261
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.17287.a.A1.PS00979 Full length( MytuD.17287.a ) 1 261

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.3263
RefSeq: NP_217444.1
TubercuList: Rv2928
MTB Network Portal: Rv2928
UniProt: P9WQD5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLARHGPRYG GSVNGHSDDS SGDAKQAAPT LYIFPHAGGT AKDYVAFSRE FSADVKRIAV QYPGQHDRSG LPPLESIPTL ADEIFAMMKP SARIDDPVAF FGHSMGGMLA FEVALRYQSA GHRVLAFFVS ACSAPGHIRY KQLQDLSDRE MLDLFTRMTG MNPDFFTDDE FFVGALPTLR AVRAIAGYSC PPETKLSCPI YAFIGDKDWI ATQDDMDPWR DRTTEEFSIR VFPGDHFYLN DNLPELVSDI EDKTLQWHDR A
NT Sequence
ATGCTGGCCC GTCACGGACC ACGCTATGGT GGCTCGGTGA ACGGGCACTC AGACGACAGT AGCGGCGACG CGAAGCAAGC CGCACCCACG CTGTATATTT TCCCGCATGC CGGCGGCACC GCGAAAGACT ATGTCGCATT TTCCCGAGAA TTTTCCGCCG ACGTAAAGCG GATTGCTGTC CAATACCCCG GCCAGCACGA TCGTTCTGGC CTGCCACCGC TTGAGAGTAT TCCCACCCTC GCTGACGAAA TCTTTGCAAT GATGAAACCG TCGGCTCGGA TCGACGATCC GGTGGCATTC TTTGGGCACA GTATGGGCGG AATGCTAGCC TTCGAAGTAG CGTTGCGATA CCAATCGGCG GGCCATCGAG TCCTGGCATT CTTTGTGTCG GCCTGCTCAG CACCGGGTCA TATCAGATAC AAGCAGCTCC AAGATTTATC AGATCGCGAG ATGTTGGACT TGTTCACCCG AATGACAGGA ATGAATCCAG ATTTCTTTAC CGACGACGAA TTTTTCGTTG GAGCGCTACC CACGTTGCGA GCGGTCCGAG CCATCGCCGG TTATTCCTGC CCACCAGAGA CGAAGCTCTC GTGTCCGATT TATGCCTTTA TCGGAGATAA AGATTGGATC GCAACGCAAG ACGACATGGA TCCGTGGCGC GATCGGACGA CGGAAGAGTT CTCTATCCGT GTATTCCCTG GGGATCACTT CTACCTCAAC GACAATTTGC CAGAGCTAGT CAGCGACATA GAAGACAAAA CACTCCAATG GCATGATCGA GCT
Details for MytuD.17287.a.A1.GE30713
HARVESTED ON: 11/9/2010
SEQUENCED ON: 11/15/2010
EXPECTED MW: 31kDa
OBSERVED MW: 31kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMLARHGPRY GGSVNGHSDD SSGDAKQAAP TLYIFPHAGG TAKDYVAFSR EFSADVKRIA VQYPGQHDRS GLPPLESIPT LADEIFAMMK PSARIDDPVA FFGHSMGGML AFEVALRYQS AGHRVLAFFV SACSAPGHIR YKQLQDLSDR EMLDLFTRMT GMNPDFFTDD EFFVGALPTL RAVRAIAGYS CPPETKLSCP IYAFIGDKDW IATQDDMDPW RDRTTEEFSI RVFPGDHFYL NDNLPELVSD IEDKTLQWHD RA
Validated NT Sequence
ttattaagct cgatcatgcc attggagtgt tttgtcttct atgtcgctga ctagctctgg caaattgtcg ttgaggtaga agtgatcccc agggaataca cggatagaga actcttccgt cgtccgatcg cgccacggat ccatgtcgtc ttgcgttgcg atccaatctt tatctccgat aaaggcataa atcggacacg agagcttcgt ctctggtggg caggaataac cggcgatggc tcggaccgct cgcaacgtgg gtagcgctcc aacgaaaaat tcgtcgtcgg taaagaaatc tggattcatt cctgtcattc gggtgaacaa gtccaacatc tcgcgatctg ataaatcttg gagctgcttg tatctgatat gacccggtgc tgagcaggcc gacacaaaga atgccaggac tcgatggccc gccgattggt atcgcaacgc tacttcgaag gctagcattc cgcccatact gtgcccaaag aatgccaccg gatcgtcgat ccgagccgac ggtttcatca ttgcaaagat ttcgtcagcg agggtgggaa tactctcaag cggtggcagg ccagaacgat cgtgctggcc ggggtattgg acagcaatcc gctttacgtc ggcggaaaat tctcgggaaa atgcgacata gtctttcgcg gtgccgccgg catgcgggaa aatatacagc gtgggtgcgg cttgcttcgc gtcgccgcta ctgtcgtctg agtgcccgtt caccgagcca ccatagcgtg gtccgtgacg ggccagcatc gaaccaggac cctgggtctg agcttccagg gtacccatat gatggtgatg gtgatgagcc at
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLARHGPRY GGSVNGHSDD SSGDAKQAAP TLYIFPHAGG TAKDYVAFSR EFSADVKRIA VQYPGQHDRS GLPPLESIPT LADEIFAMMK PSARIDDPVA FFGHSMGGML AFEVALRYQS AGHRVLAFFV SACSAPGHIR YKQLQDLSDR EMLDLFTRMT GMNPDFFTDD EFFVGALPTL RAVRAIAGYS CPPETKLSCP IYAFIGDKDW IATQDDMDPW RDRTTEEFSI RVFPGDHFYL NDNLPELVSD IEDKTLQWHD RA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gctggcccgt cacggaccac gctatggtgg ctcggtgaac gggcactcag acgacagtag cggcgacgcg aagcaagccg cacccacgct gtatattttc ccgcatgccg gcggcaccgc gaaagactat gtcgcatttt cccgagaatt ttccgccgac gtaaagcgga ttgctgtcca ataccccggc cagcacgatc gttctggcct gccaccgctt gagagtattc ccaccctcgc tgacgaaatc tttgcaatga tgaaaccgtc ggctcggatc gacgatccgg tggcattctt tgggcacagt atgggcggaa tgctagcctt cgaagtagcg ttgcgatacc aatcggcggg ccatcgagtc ctggcattct ttgtgtcggc ctgctcagca ccgggtcata tcagatacaa gcagctccaa gatttatcag atcgcgagat gttggacttg ttcacccgaa tgacaggaat gaatccagat ttctttaccg acgacgaatt tttcgttgga gcgctaccca cgttgcgagc ggtccgagcc atcgccggtt attcctgccc accagagacg aagctctcgt gtccgattta tgcctttatc ggagataaag attggatcgc aacgcaagac gacatggatc cgtggcgcga tcggacgacg gaagagttct ctatccgtgt attccctggg gatcacttct acctcaacga caatttgcca gagctagtca gcgacataga agacaaaaca ctccaatggc atgatcgagc ttaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa
Details for MytuD.17287.a.A1.PS00979
PURIFICATION DATe: 2/17/2011
CONCENTRATION: 5.4mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMLARHGPRY GGSVNGHSDD SSGDAKQAAP TLYIFPHAGG TAKDYVAFSR EFSADVKRIA VQYPGQHDRS GLPPLESIPT LADEIFAMMK PSARIDDPVA FFGHSMGGML AFEVALRYQS AGHRVLAFFV SACSAPGHIR YKQLQDLSDR EMLDLFTRMT GMNPDFFTDD EFFVGALPTL RAVRAIAGYS CPPETKLSCP IYAFIGDKDW IATQDDMDPW RDRTTEEFSI RVFPGDHFYL NDNLPELVSD IEDKTLQWHD RA
Validated NT Sequence
ttattaagct cgatcatgcc attggagtgt tttgtcttct atgtcgctga ctagctctgg caaattgtcg ttgaggtaga agtgatcccc agggaataca cggatagaga actcttccgt cgtccgatcg cgccacggat ccatgtcgtc ttgcgttgcg atccaatctt tatctccgat aaaggcataa atcggacacg agagcttcgt ctctggtggg caggaataac cggcgatggc tcggaccgct cgcaacgtgg gtagcgctcc aacgaaaaat tcgtcgtcgg taaagaaatc tggattcatt cctgtcattc gggtgaacaa gtccaacatc tcgcgatctg ataaatcttg gagctgcttg tatctgatat gacccggtgc tgagcaggcc gacacaaaga atgccaggac tcgatggccc gccgattggt atcgcaacgc tacttcgaag gctagcattc cgcccatact gtgcccaaag aatgccaccg gatcgtcgat ccgagccgac ggtttcatca ttgcaaagat ttcgtcagcg agggtgggaa tactctcaag cggtggcagg ccagaacgat cgtgctggcc ggggtattgg acagcaatcc gctttacgtc ggcggaaaat tctcgggaaa atgcgacata gtctttcgcg gtgccgccgg catgcgggaa aatatacagc gtgggtgcgg cttgcttcgc gtcgccgcta ctgtcgtctg agtgcccgtt caccgagcca ccatagcgtg gtccgtgacg ggccagcatc gaaccaggac cctgggtctg agcttccagg gtacccatat gatggtgatg gtgatgagcc at
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLARHGPRY GGSVNGHSDD SSGDAKQAAP TLYIFPHAGG TAKDYVAFSR EFSADVKRIA VQYPGQHDRS GLPPLESIPT LADEIFAMMK PSARIDDPVA FFGHSMGGML AFEVALRYQS AGHRVLAFFV SACSAPGHIR YKQLQDLSDR EMLDLFTRMT GMNPDFFTDD EFFVGALPTL RAVRAIAGYS CPPETKLSCP IYAFIGDKDW IATQDDMDPW RDRTTEEFSI RVFPGDHFYL NDNLPELVSD IEDKTLQWHD RA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCTGGCCCGT CACGGACCAC GCTATGGTGG CTCGGTGAAC GGGCACTCAG ACGACAGTAG CGGCGACGCG AAGCAAGCCG CACCCACGCT GTATATTTTC CCGCATGCCG GCGGCACCGC GAAAGACTAT GTCGCATTTT CCCGAGAATT TTCCGCCGAC GTAAAGCGGA TTGCTGTCCA ATACCCCGGC CAGCACGATC GTTCTGGCCT GCCACCGCTT GAGAGTATTC CCACCCTCGC TGACGAAATC TTTGCAATGA TGAAACCGTC GGCTCGGATC GACGATCCGG TGGCATTCTT TGGGCACAGT ATGGGCGGAA TGCTAGCCTT CGAAGTAGCG TTGCGATACC AATCGGCGGG CCATCGAGTC CTGGCATTCT TTGTGTCGGC CTGCTCAGCA CCGGGTCATA TCAGATACAA GCAGCTCCAA GATTTATCAG ATCGCGAGAT GTTGGACTTG TTCACCCGAA TGACAGGAAT GAATCCAGAT TTCTTTACCG ACGACGAATT TTTCGTTGGA GCGCTACCCA CGTTGCGAGC GGTCCGAGCC ATCGCCGGTT ATTCCTGCCC ACCAGAGACG AAGCTCTCGT GTCCGATTTA TGCCTTTATC GGAGATAAAG ATTGGATCGC AACGCAAGAC GACATGGATC CGTGGCGCGA TCGGACGACG GAAGAGTTCT CTATCCGTGT ATTCCCTGGG GATCACTTCT ACCTCAACGA CAATTTGCCA GAGCTAGTCA GCGACATAGA AGACAAAACA CTCCAATGGC ATGATCGAGC TTAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA