MytuD.17259.a

helicase-nuclease AddB Rv3202c

CENTER ID: MytuD.17259.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.17259.a.A2.GE30107 UvrD-like helicase 4 324
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.3580
RefSeq: NP_217718.1
TubercuList: Rv3202c
MTB Network Portal: Rv3202c
UniProt: O53348

Orthologues for Rv3202c

Species % Identity % Coverage Clones Proteins Structures
marinum 77 100 0 0 0
smegmatis 62 99 1 0 0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSHIWGVEAG AALAPGLRGP VLVLGGPGTG KSTLLVEAAV AHIGAGTDPE SVLLLTGSGR MGMRARSALT TALLRSRTNG PCRAAIREPV VRTVHSYAYA VLRKAAQRAG DALPRLLTSA EQDAIIRELL AGDAEDGPAA TTTWPAHLRP ALTTAGFATE LRNLLARCAE RGLDPLELQQ LGRRRGRPEW IAAGQFAQRY EQVMLLRGAV GLAAPQATAP ALSAAELVGA ALEAFAVDPE LLAAERARVR TLLVDDAQQL DPQAARLVRM LAAGTELALI AGDPNQAVFG FRGGEPTGLL ADDPPPAGGA PIPSVTLTVS HRCAPAVARA VTGIARRLPG RSVGRRIEGT GTEVGSVTVR LAGSAHAEAA MIADALRRAH LIDGVPWSQM AVIVRSVPRA VRLPRALAAA GVPVAPPAVG GPLSAEPAVR ALLTVLEATA DGLDGDQALL LLTGPIGGVD PVSLRQLRRT LQRARPGQTS RKFGDLLVEV LGGDAPPSGP GSRALRRVRA VLTAAARCHR SGSLGGQDPR HTLWAAWQRS GLQRRWLAAS EHGGAAAVQA TRDLETVTAL FDITDHYVSR TSGASLRGLV EHVTALQLPV VRPEPAAPTE QVMVLSAHAA LGHEWDLVVI AGLQDGLWPN TVPRGGVLGT QRLLDELDGV TKDASMRAPL LAEERRLLVT AMGRARRRLL VTAVDSDAGG GGHEAVLPSA FFFEIAQWAD GDGEPVAMQP VSAPRVLSAA AVVGRLRVVV CAPACAVDDA DRDCAATQLA RLAKAGVPGA DPSEWHGLAP VSTSDPLCDS DDLVTLTPST LQALNDCPLR WLAERHGGTN TRELPSAVGS VLHALFAEPG RSESQLLAEL DRVWGHLPFG AQWYSANELA RHRAMIQAFV QWRAQSRSEL TEVGVEVDID GALEDGSGQA RKIRLRGRAD RLERDPAGRL VIVDIKTGKT PVSKDDAQQH AQLAMYQLAV AEGLVRAGDE PGGARLVYVG KSGAAGVAER KQDPLTPAAR DEWRNLVRQL AAATAGPQFI ARRNDGCTHC PLRPGCPAHV RGSAP
NT Sequence
atgtcacata tctggggtgt cgaggcgggt gcggccctcg cgccgggttt gcgcggtccg gtgcttgtgc tgggcggccc gggcaccggt aagagcacac tgttggttga ggccgcggtc gctcacatcg gcgccggcac cgacccggag tcggttctgc tgctgaccgg ttccggccga atgggcatgc gggcccgcag tgcgctgacg acggcgctgc tgcggtcgcg caccaacggc ccttgccggg cggcgatccg cgaaccggtg gtacgcaccg tgcacagcta cgcctatgcg gtcttgcgca aggccgcaca gcgcgccggt gacgccttgc cgcggctgct taccagcgcc gagcaggacg ccatcattcg ggaactgctg gccggggacg ccgaagacgg accggccgcc accaccacct ggcctgcgca tctgcggccc gcgctgacta ccgccggctt cgccaccgag ctgcgaaacc tgttggcacg ttgcgccgaa cgcggcctgg acccgctgga gttgcagcaa ctgggccgtc gccgcggccg tccggaatgg atcgccgccg gtcaattcgc ccagcggtac gagcaggtga tgttgttgcg gggtgcggtg gggctggcgg cgccgcaggc cacggcgccg gcgctgagtg ccgccgaact ggtgggggca gctttggagg ccttcgcggt cgatcctgag ttactggccg ccgaacgcgc ccgggtccgg acccttttgg tcgacgacgc ccaacaactt gatccgcagg cggcacgcct ggtccggatg ctggcggcgg gcaccgagct ggccctgatc gccggtgatc cgaaccaagc ggtgttcggg ttccgcggcg gcgagcccac cggcctgctg gccgacgatc cgccgccagc aggtggtgcc cccattccgt cggtgacgtt gacggtctct catcggtgtg cacccgccgt ggcgcgggcc gtcaccggca tcgcacgacg gctaccgggt cgaagcgtcg gtcggcgaat cgagggcacc gggaccgagg tcggatcggt gacggtgcgc ctggccggtt cggcgcacgc tgaggcagcg atgattgccg acgcgctgcg acgcgcgcac ctgatcgatg gggtgccttg gtcgcagatg gcggtgatcg tcaggtcggt gccgcgcgct gtgcggttgc cgcgcgctct ggccgccgcc ggggtgccgg tggccccacc tgcagttggc gggccgttgt ccgcggagcc ggcggtacgg gcgctgctca cggtgctcga ggcgacggct gacgggctcg acggcgacca ggcgctgctc ctgctcaccg ggccgattgg tggtgtggac ccggtatcac ttcgccagct gcgccggact ctgcaacgcg cacgtcccgg ccagacttcg aggaagttcg gcgacctgct ggtggaagtg ctcggcggcg acgcgccgcc atcggggcca gggtcgcggg cactgcggcg cgtgcgtgcc gtgctgaccg cggccgcgcg ctgccaccgc tcaggaagtc tgggtggcca ggatccgcgc cacacgctat gggctgcctg gcaacggtcg ggtctgcaac gccgctggct ggcggccagc gagcatggtg gtgcggcggc tgtccaggcc acccgggacc tggaaacggt gaccgccttg ttcgacatca ccgaccatta cgtgtcccgc acctcgggtg cgtccctgcg cggactcgtc gagcacgtca ccgcgctgca gctgccggtt gtgcgacccg aaccggcagc cccgacagag caggtcatgg tccttagcgc acatgccgcg ctgggacacg aatgggatct ggtagttatc gccggcctac aggacggctt gtggcccaac acggttccac gtggcggtgt gcttggcacc cagcggctgc tggacgaact cgacggtgtc acgaaggatg cctcgatgcg cgcgccgttg ctggccgagg agcgccggct gctggtgacc gcgatggggc gggcccggcg gcggttgctg gtgaccgccg tagatagcga cgcgggcggg ggcgggcacg aggcggtgct gccatcggcg ttctttttcg agattgccca gtgggccgac ggcgatggcg aacccgtcgc gatgcagccg gtctcggcgc cgcgcgtgtt gtcggctgcg gcggtggtag gccggttacg cgttgtggtg tgcgcgcctg cctgcgcggt agatgacgcc gatcgcgatt gtgcggcaac gcaattggcc cggttggcca aagccggtgt gccaggtgcc gacccgtccg agtggcatgg cctggccccg gtcagcacga gtgatccgtt gtgcgatagc gacgatctcg tcacgttgac gccgtcgact ttgcaggcac tcaacgactg cccactgcgc tggctggccg agcggcacgg cggaacaaac acccgcgagc tgccgtctgc cgtcggatcg gtgttgcacg cactgtttgc cgagccgggc aggagcgaat cccaactgct ggctgaactg gaccgggtgt ggggccatct gccgttcggc gctcagtggt attcggccaa cgagcttgcc cggcaccgcg cgatgatcca ggcgttcgta cagtggcgag ctcagagtcg atccgagctg accgaagttg gcgtggaggt cgacatcgat ggggccctcg aggacggtag cggtcaagcc agaaaaattc ggttgcgtgg ccgggcggat cggctggaac gcgacccggc cggccgcctg gtgatcgtcg acatcaaaac cggcaagaca ccggtcagca aggacgatgc ccagcagcac gcccagctgg cgatgtatca gctggcggtg gccgaaggct tggtgcgcgc cggggacgag cccggcggtg cccggctggt ctatgtcggc aaaagcgggg ccgctggcgt cgccgaacgc aaacaggatc cactcacacc ggccgcgcgc gacgaatggc gcaacctcgt ccggcagctg gccgcggcga cagccggtcc ccagttcatc gctcggcgca acgacgggtg cacccactgc ccgctgcggc cgggctgtcc ggcccacgtc cgagggtcgg cgccatga
Details for MytuD.17259.a.A2.GE30107
HARVESTED ON: 8/15/2010
SEQUENCED ON: 8/25/2010
EXPECTED MW: 35kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAQTQG PGSMIWGVEA GAALAPGLRG PVLVLGGPGT GKSTLLVEAA VAHIGAGTDP ESVLLLTGSG RMGMRARSAL TTALLRSRTN GPCRAAIREP VVRTVHSYAY AVLRKAAQRA GDALPRLLTS AEQDAIIREL LAGDAEDGPA ATTTWPAHLR PALTTAGFAT ELRNLLARCA ERGLDPLELQ QLGRRRGRPE WIAAGQFAQR YEQVMLLRGA VGLAAPQATA PALSAAELVG AALEAFAVDP ELLAAERARV RTLLVDDAQQ LDPQAARLVR MLAAGTELAL IAGDPNQAVF GFRGGEPTGL LADDPPPAGG APIPSVTLTV SHRC
Validated NT Sequence
gcacaccgat gagagaccgt caacgtcacc gacggaatgg gggcaccacc tgctggcggc ggatcgtcgg ccagcaggcc ggtgggctcg ccgccgcgga acccgaacac cgcttggttc ggatcaccgg cgatcagggc cagctcggtg cccgccgcca gcatccggac caggcgtgcc gcctgcggat caagttgttg ggcgtcgtcg accaaaaggg tccggacccg ggcgcgttcg gcggccagta actcaggatc gaccgcgaag gcctccaaag ctgcccccac cagttcggcg gcactcagcg ccggcgccgt ggcctgcggc gccgccagcc ccaccgcacc ccgcaacaac atcacctgct cgtaccgctg ggcgaattga ccggcggcga tccattccgg acggccgcgg cgacggccca gttgctgcaa ctccagcggg tccaggccgc gttcggcgca acgtgccaac aggtttcgca gctcggtggc gaagccggcg gtagtcagcg cgggccgcag atgcgcaggc caggtggtgg tggcggccgg tccgtcttcg gcgtccccgg ccagcagttc ccgaatgatg gcgtcctgct cggcgctggt aagcagccgc ggcaaggcgt caccggcgcg ctgtgcggcc ttgcgcaaga ccgcataggc gtagctgtgc acggtgcgta ccaccggttc gcggatcgcc gcccggcaag ggccgttggt gcgcgaccgc agcagcgccg tcgtcagcgc actgcgggcc cgcatgccca ttcggccgga accggtcagc agcagaaccg actccgggtc ggtgccggcg ccgatgtgag cgaccgcggc ctcaaccaac agtgtgctct taccggtgcc cgggccgccc agcacaagca ccggaccgcg caaacccggc gcgagggccg cacccgcctc gacaccccag atcatcgaac caggaccctg ggtctgagct tccagggtac ccatatgatg gtganggtga tgagccangg tatntcncct tcttaa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMIWGVEAGA ALAPGLRGPV LVLGGPGTGK STLLVEAAVA HIGAGTDPES VLLLTGSGRM GMRARSALTT ALLRSRTNGP CRAAIREPVV RTVHSYAYAV LRKAAQRAGD ALPRLLTSAE QDAIIRELLA GDAEDGPAAT TTWPAHLRPA LTTAGFATEL RNLLARCAER GLDPLELQQL GRRRGRPEWI AAGQFAQRYE QVMLLRGAVG LAAPQATAPA LSAAELVGAA LEAFAVDPEL LAAERARVRT LLVDDAQQLD PQAARLVRML AAGTELALIA GDPNQAVFGF RGGEPTGLLA DDPPPAGGAP IPSVTLTVSH RCA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gatctggggt gtcgaggcgg gtgcggccct cgcgccgggt ttgcgcggtc cggtgcttgt gctgggcggc ccgggcaccg gtaagagcac actgttggtt gaggccgcgg tcgctcacat cggcgccggc accgacccgg agtcggttct gctgctgacc ggttccggcc gaatgggcat gcgggcccgc agtgcgctga cgacggcgct gctgcggtcg cgcaccaacg gcccttgccg ggcggcgatc cgcgaaccgg tggtacgcac cgtgcacagc tacgcctatg cggtcttgcg caaggccgca cagcgcgccg gtgacgcctt gccgcggctg cttaccagcg ccgagcagga cgccatcatt cgggaactgc tggccgggga cgccgaagac ggaccggccg ccaccaccac ctggcctgcg catctgcggc ccgcgctgac taccgccggc ttcgccaccg agctgcgaaa cctgttggca cgttgcgccg aacgcggcct ggacccgctg gagttgcagc aactgggccg tcgccgcggc cgtccggaat ggatcgccgc cggtcaattc gcccagcggt acgagcaggt gatgttgttg cggggtgcgg tggggctggc ggcgccgcag gccacggcgc cggcgctgag tgccgccgaa ctggtggggg cagctttgga ggccttcgcg gtcgatcctg agttactggc cgccgaacgc gcccgggtcc ggaccctttt ggtcgacgac gcccaacaac ttgatccgca ggcggcacgc ctggtccgga tgctggcggc gggcaccgag ctggccctga tcgccggtga tccgaaccaa gcggtgttcg ggttccgcgg cggcgagccc accggcctgc tggccgacga tccgccgcca gcaggtggtg cccccattcc gtcggtgacg ttgacggtct ctcatcggtg tgcaaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa