MytuD.17208.a

RNA polymerase sigma factor

CENTER ID: MytuD.17208.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.17208.a.A2.GE30711 Mature protein( MytuD.17208.a ) 24 370
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.206
RefSeq: NP_214696.1
TubercuList: Rv0182c
MTB Network Portal: Rv0182c
UniProt: O07426

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRTSPMPAKF RSVRVVVITG SVTAAPVRVS ETLRRLIDVS VLAENSGREP ADERRGDFSA HTEPYRRELL AHCYRMTGSL HDAEDLVQET LLRAWKAYEG FAGKSSLRTW LHRIATNTCL TALEGRRRRP LPTGLGRPSA DPSGELVERR EVSWLEPLPD VTDDPADPST IVGNRESVRL AFVAALQHLS PRQRAVLLLR DVLQWKSAEV ADAIGTSTVA VNSLLQRARS QLQTVRPSAA DRLSAPDSPE AQDLLARYIA AFEAYDIDRL VELFTAEAIW EMPPYTGWYQ GAQAIVTLIH QQCPAYSPGD MRLISLIANG QPAAAMYMRA GDVHLPFQLH VLDMAADRVS HVVAFLDTTL FPKFGLPDSL
NT Sequence
ATGCGCACAT CGCCGATGCC CGCGAAATTC CGTTCAGTCC GGGTGGTAGT CATTACCGGG AGCGTAACGG CTGCTCCGGT GAGAGTGTCG GAGACTCTGC GTAGGCTCAT TGACGTGAGT GTGCTCGCAG AAAACTCTGG CCGCGAGCCC GCCGACGAAC GGCGCGGGGA CTTCTCCGCC CACACCGAGC CCTACCGGCG TGAACTGCTC GCACACTGCT ATCGCATGAC TGGCTCGCTG CACGACGCGG AAGACCTTGT TCAGGAGACG CTGCTGCGGG CCTGGAAGGC CTATGAGGGC TTCGCGGGCA AGTCCTCGCT ACGCACCTGG CTGCATCGGA TCGCCACCAA CACCTGCCTG ACGGCGTTGG AGGGCCGTCG ACGCCGGCCG TTGCCGACGG GGCTCGGGCG GCCGAGTGCC GATCCGTCCG GGGAGTTGGT GGAACGTCGA GAGGTGTCCT GGCTGGAGCC GCTGCCGGAC GTGACGGACG ACCCTGCTGA TCCCTCAACG ATCGTCGGGA ACCGGGAATC GGTGCGGTTG GCCTTTGTGG CGGCTCTGCA GCACCTCTCA CCCCGGCAGC GAGCCGTACT GCTGCTGCGC GACGTGCTGC AGTGGAAATC GGCCGAGGTG GCCGACGCGA TCGGTACCAG CACCGTCGCC GTCAATAGCC TGCTGCAGCG GGCCCGCTCT CAGCTGCAGA CGGTCCGGCC CAGCGCCGCC GACCGGTTGT CCGCGCCGGA TTCACCCGAG GCGCAAGACC TGTTGGCCCG CTATATCGCC GCGTTCGAGG CTTATGACAT CGACCGGTTG GTCGAGCTGT TCACCGCCGA GGCGATCTGG GAAATGCCGC CGTATACCGG CTGGTACCAG GGCGCACAGG CCATCGTCAC CCTCATTCAC CAACAGTGCC CCGCCTATTC GCCTGGCGAT ATGCGCCTGA TTTCGTTGAT CGCCAACGGC CAGCCCGCCG CGGCCATGTA CATGCGTGCC GGTGACGTGC ACCTGCCTTT TCAGCTACAC GTGCTGGACA TGGCCGCCGA CCGCGTGTCA CACGTGGTGG CCTTCCTCGA CACCACCTTG TTCCCGAAAT TCGGCCTGCC CGATTCGCTG
Details for MytuD.17208.a.A2.GE30711
HARVESTED ON: 11/9/2010
SEQUENCED ON: 8/27/2015
EXPECTED MW: 40kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAQTQG PGSMAAPVRV SETLRRLIDV SVLAENSGRE PADERRGDFS AHTEPYRREL LAHCYRMTGS LHDAEDLVQE TLLRAWKAYE GFAGKSSLRT WLHRIATNTC LTALEGRRRR PLPTGLGRPS ADPSGELVER REVSWLEPLP DVTDDPADPS TIVGNRESVR LAFVAALQHL SPRQRAVLLL RDVLQWKSAE VADAIGTSTV AVNSLLQRAR SQLQTVRPSA ADRLSAPDSP EAQDLLARYI AAFEAYDIDR LVELFTAEAI WEMPPYTGWY QGAQAIVTLI HQQCPAYSPG DMRLISLIAN GQPAAAMYMR AGDVHLPFQL HVLDMAADRV SHVVAFLDTT LFPKFGLPDS L
Validated NT Sequence
gctcatcacc atcaccatca tatgggtacc ctggaagctc agacccaggg tcctggttcg atggctgctc cggtgagagt gtcggagact ctgcgtaggc tcattgacgt gagtgtgctc gcagaaaact ctggccgcga gcccgccgac gaacggcgcg gggacttctc cgcccacacc gagccctacc ggcgtgaact gctcgcacac tgctatcgca tgactggctc gctgcacgac gcggaagacc ttgttcagga gacgctgctg cgggcctgga aggcctatga gggcttcgcg ggcaagtcct cgctacgcac ctggctgcat cggatcgcca ccaacacctg cctgacggcg ttggagggcc gtcgacgccg gccgttgccg acggggctcg ggcggccgag tgccgatccg tccggggagt tggtggaacg tcgagaggtg tcctggctgg agccgctgcc ggacgtgacg gacgaccctg ctgatccctc aacgatcgtc gggaaccggg aatcggtgcg gttggccttt gtggcggctc tgcagcacct ctcaccccgg cagcgagccg tactgctgct gcgcgacgtg ctgcagtgga aatcggccga ggtggccgac gcgatcggta ccagcaccgt cgccgtcaat agcctgctgc agcgggcccg ctctcagctg cagacggtcc ggcccagcgc cgccgaccgg ttgtccgcgc cggattcacc cgaggcgcaa gacctgttgg cccgctatat cgccgcgttc gaggcttatg acatcgaccg gttggtcgag ctgttcaccg ccgaggcgat ctgggaaatg ccgccgtata ccggctggta ccagggcgca caggccatcg tcaccctcat tcaccaacag tgccccgcct attcgcctgg cgatatgcgc ctgatttcgt tgatcgccaa cggccagccc gccgcggcca tgtacatgcg tgccggtgac gtgcacctgc cttttcagct acacgtgctg gacatggccg ccgaccgcgt gtcacacgtg gtggccttcc tcgacaccac cttgttcccg aaattcggcc tgcccgattc gctgtaataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAAPVRVSE TLRRLIDVSV LAENSGREPA DERRGDFSAH TEPYRRELLA HCYRMTGSLH DAEDLVQETL LRAWKAYEGF AGKSSLRTWL HRIATNTCLT ALEGRRRRPL PTGLGRPSAD PSGELVERRE VSWLEPLPDV TDDPADPSTI VGNRESVRLA FVAALQHLSP RQRAVLLLRD VLQWKSAEVA DAIGTSTVAV NSLLQRARSQ LQTVRPSAAD RLSAPDSPEA QDLLARYIAA FEAYDIDRLV ELFTAEAIWE MPPYTGWYQG AQAIVTLIHQ QCPAYSPGDM RLISLIANGQ PAAAMYMRAG DVHLPFQLHV LDMAADRVSH VVAFLDTTLF PKFGLPDSL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggctgctccg gtgagagtgt cggagactct gcgtaggctc attgacgtga gtgtgctcgc agaaaactct ggccgcgagc ccgccgacga acggcgcggg gacttctccg cccacaccga gccctaccgg cgtgaactgc tcgcacactg ctatcgcatg actggctcgc tgcacgacgc ggaagacctt gttcaggaga cgctgctgcg ggcctggaag gcctatgagg gcttcgcggg caagtcctcg ctacgcacct ggctgcatcg gatcgccacc aacacctgcc tgacggcgtt ggagggccgt cgacgccggc cgttgccgac ggggctcggg cggccgagtg ccgatccgtc cggggagttg gtggaacgtc gagaggtgtc ctggctggag ccgctgccgg acgtgacgga cgaccctgct gatccctcaa cgatcgtcgg gaaccgggaa tcggtgcggt tggcctttgt ggcggctctg cagcacctct caccccggca gcgagccgta ctgctgctgc gcgacgtgct gcagtggaaa tcggccgagg tggccgacgc gatcggtacc agcaccgtcg ccgtcaatag cctgctgcag cgggcccgct ctcagctgca gacggtccgg cccagcgccg ccgaccggtt gtccgcgccg gattcacccg aggcgcaaga cctgttggcc cgctatatcg ccgcgttcga ggcttatgac atcgaccggt tggtcgagct gttcaccgcc gaggcgatct gggaaatgcc gccgtatacc ggctggtacc agggcgcaca ggccatcgtc accctcattc accaacagtg ccccgcctat tcgcctggcg atatgcgcct gatttcgttg atcgccaacg gccagcccgc cgcggccatg tacatgcgtg ccggtgacgt gcacctgcct tttcagctac acgtgctgga catggccgcc gaccgcgtgt cacacgtggt ggccttcctc gacaccacct tgttcccgaa attcggcctg cccgattcgc tgtaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa