MytuD.17056.a

PROBABLE CONSERVED MEMBRANE PROTEIN Rv0347

CENTER ID: MytuD.17056.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:
IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
MytuD.17056.a.A2.GE27364 soluble domain 66 328
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_239112
PATRIC ID: fig|83332.12.peg.382
RefSeq: NP_214861.1
TubercuList: Rv0347
MTB Network Portal: Rv0347
UniProt: O06298
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPGARELTLR VERGALFRRR WAASAASSAR AAIRRDPRRC ALGTRPRWVS FLVIVLVIMN VVTAHPKYPN DPLALVLIEL RHPRTEPPVP SAISILKEEL ARWTPILEQE EVRQVNLETG EHTAHSQKKL VARDRRTAIT FRPDAMTLEV TDYPGWEEFR SIVHAMVTAR QDVAPVDGCI RIGLRYINEI RASLAEPSGW AYWVAESLLG PGTQLADLKL TTTAQRHVIQ CEGPEPGDSL TLRYAGARGA VIQSTPFLQR LKEPPAEGDF FLIDIDSAWS DPCKGIPALD AHLVDEVAER LHTPIGPLFE SLITSELRTK VLQQPGQE
NT Sequence
gtgcccggcg cgcgcgagtt gacgctgcgc gtcgagcgcg gggctctatt tcggcgtcga tgggcagcat cggcagcgtc atcagctcgc gcagcaattc gtcgtgatcc gcggcgctgc gcgctgggta cccggcctcg atgggtatca tttttggtta tcgttctggt tatcatgaat gttgtgacgg cccatcccaa gtacccgaat gaccctcttg cgctggtatt gattgaactg cgccatccgc ggaccgagcc gccggtgcca tctgctatct ccatcctgaa ggaggagctg gcgcgatgga ctcccatact cgaacaggag gaggtgcggc aggtcaacct agaaacgggc gaacataccg cacactcaca gaagaagctc gttgcccgtg atcgccgcac cgcgatcacg tttcgacccg acgccatgac cctcgaagtc accgactacc cgggctggga ggagtttcgg tccatcgttc acgcgatggt cacagcccgc caggacgtgg ccccagtcga tggctgcatc cggatcggtc tgcgctacat caacgagatt cgggcatcgc tggcggagcc atccggctgg gcgtactggg tggcggaaag tctcctcggg cctgggacac agcttgccga tctcaaactc accaccaccg cgcaacggca cgtcattcag tgcgaaggcc cggagccagg cgactccttg acactgaggt acgccggtgc gcgcggcgcg gtcatccagt caaccccgtt tctccagcgg ttgaaagaac ctccggcaga aggagatttc ttcctcatcg atatcgacag cgcgtggagc gacccctgca agggcatccc agcgctcgac gcccacctgg tggacgaggt cgccgaaagg ctccacacac ccatcggccc actgttcgaa tcgctgataa cttccgaact ccgtacaaag gtgctgcaac aacctgggca ggagtga
Details for MytuD.17056.a.A2.GE27364
HARVESTED ON: 10/23/2009
SEQUENCED ON: 10/29/2009
EXPECTED MW: 31kDa
OBSERVED MW: 33kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMPKYPNDPL ALVLIELRHP RTEPPVPSAI SILKEELARW TPILEQEEVR QVNLETGEHT AHSQKKLVAR DRRTAITFRP DAMTLEVTDY PGWEEFRSIV HAMVTARQDV APVDGCIRIG LRYINEIRAS LAEPSGWAYW VAESLLGPGT QLADLKLTTT AQRHVIQCEG PEPGDSLTLR YAGARGAVIQ STPFLQRLKE PPAEGDFFLI DIDSAWSDPC KGIPALDAHL VDEVAERLHT PIGPLFESLI TSELRTKVLQ QP
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgccca agtacccgaa tgaccctctt gcgctggtat tgattgaact gcgccatccg cggaccgagc cgccggtgcc atctgctatc tccatcctga aggaggagct ggcgcgatgg actcccatac tcgaacagga ggaggtgcgg caggtcaacc tagaaacggg cgaacatacc gcacactcac agaagaagct cgttgcccgt gatcgccgca ccgcgatcac gtttcgaccc gacgccatga ccctcgaagt caccgactac ccgggctggg aggagtttcg gtccatcgtt cacgcgatgg tcacagcccg ccaggacgtg gccccagtcg atggctgcat ccggatcggt ctgcgctaca tcaacgagat tcgggcatcg ctggcggagc catccggctg ggcgtactgg gtggcggaaa gtctcctcgg gcctgggaca cagcttgccg atctcaaact caccaccacc gcgcaacggc acgtcattca gtgcgaaggc ccggagccag gcgactcctt gacactgagg tacgccggtg cgcgcggcgc ggtcatccag tcaaccccgt ttctccagcg gttgaaagaa cctccggcag aaggagattt cttcctcatc gatatcgaca gcgcgtggag cgacccctgc aagggcatcc cagcgctcga cgcccacctg gtggacgagg tcgccgaaag gctccacaca cccatcggcc cactgttcga atcgctgata acttccgaac tccgtacaaa ggtgctgcaa caacctggg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMPKYPNDPL ALVLIELRHP RTEPPVPSAI SILKEELARW TPILEQEEVR QVNLETGEHT AHSQKKLVAR DRRTAITFRP DAMTLEVTDY PGWEEFRSIV HAMVTARQDV APVDGCIRIG LRYINEIRAS LAEPSGWAYW VAESLLGPGT QLADLKLTTT AQRHVIQCEG PEPGDSLTLR YAGARGAVIQ STPFLQRLKE PPAEGDFFLI DIDSAWSDPC KGIPALDAHL VDEVAERLHT PIGPLFESLI TSELRTKVLQ QPGQE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcccaagtac ccgaatgacc ctcttgcgct ggtattgatt gaactgcgcc atccgcggac cgagccgccg gtgccatctg ctatctccat cctgaaggag gagctggcgc gatggactcc catactcgaa caggaggagg tgcggcaggt caacctagaa acgggcgaac ataccgcaca ctcacagaag aagctcgttg cccgtgatcg ccgcaccgcg atcacgtttc gacccgacgc catgaccctc gaagtcaccg actacccggg ctgggaggag tttcggtcca tcgttcacgc gatggtcaca gcccgccagg acgtggcccc agtcgatggc tgcatccgga tcggtctgcg ctacatcaac gagattcggg catcgctggc ggagccatcc ggctgggcgt actgggtggc ggaaagtctc ctcgggcctg ggacacagct tgccgatctc aaactcacca ccaccgcgca acggcacgtc attcagtgcg aaggcccgga gccaggcgac tccttgacac tgaggtacgc cggtgcgcgc ggcgcggtca tccagtcaac cccgtttctc cagcggttga aagaacctcc ggcagaagga gatttcttcc tcatcgatat cgacagcgcg tggagcgacc cctgcaaggg catcccagcg ctcgacgccc acctggtgga cgaggtcgcc gaaaggctcc acacacccat cggcccactg ttcgaatcgc tgataacttc cgaactccgt acaaaggtgc tgcaacaacc tgggcaggag aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa