MytuD.16715.a

BETA LACTAMASE LIKE PROTEIN

CENTER ID: MytuD.16715.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.16715.a.MB2.GE30480 Mature protein( MytuD.16715.a ) 21 272
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_138499
PATRIC ID: fig|83332.12.peg.448
RefSeq: NP_214920.1
TubercuList: Rv0406c
MTB Network Portal: Rv0406c
UniProt: O86336
Orthologues for Rv0406c
Species % Identity % Coverage Clones Proteins Structures
marinum 75 90 0 0 0
leprae 75 89 1 0 0
avium 74 90 1 0 0
paratuberculosis 73 90 1 0 0
smegmatis 57 89 0 0 0
abscessus 52 89 0 0 0
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVATRGTRLA ALALAPRLAG MAELVQITDK VHLARGHAVN WVLVTDDTGV LLIDAGYPGD RAEVLASLNK LGYTPGDVRA IVLTHAHIDH LGSAIWFARE HSTPVYCHAE EVGHAKREYR ENASVFDVAL RSWRPRVAVW GIHLLRRGGL TGDGIPTAQP LTAEAAAGLP GQPMAIFTPG HTSGHCSYVV DGVLASGDAL ITGHPMLRHR GPQLLPAVFS HSQQNSIRSL AALALLETNI LAPGHGELWH GPIRKATDEA LERAQKSNHV FR
NT Sequence
ATGGTCGCGA CCCGCGGCAC CCGGCTTGCC GCGCTCGCGC TCGCTCCTAG ACTCGCGGGC ATGGCGGAGC TTGTTCAGAT CACCGACAAG GTTCACCTCG CTCGCGGCCA CGCGGTCAAC TGGGTGCTAG TCACCGACGA CACCGGCGTG CTGCTGATCG ACGCCGGCTA TCCCGGCGAC CGTGCGGAGG TGCTGGCCTC GCTGAACAAA CTGGGCTACA CACCGGGTGA CGTGCGCGCC ATCGTGCTCA CCCACGCGCA CATCGACCAC CTGGGCTCGG CAATCTGGTT TGCTCGTGAG CACAGCACGC CGGTGTACTG CCACGCCGAG GAGGTAGGTC ACGCCAAGCG GGAATACCGG GAGAATGCCT CGGTTTTCGA TGTTGCGCTG CGCAGCTGGC GGCCCCGTGT GGCGGTCTGG GGTATTCACT TGCTTCGCCG CGGCGGCCTG ACCGGCGACG GCATTCCGAC CGCCCAGCCG CTGACCGCCG AAGCGGCCGC CGGGCTGCCG GGTCAACCGA TGGCGATTTT CACCCCGGGA CACACCAGCG GTCACTGCTC GTATGTCGTC GACGGTGTGC TGGCCAGCGG CGATGCGCTG ATCACTGGTC ATCCGATGTT GCGTCACCGC GGGCCACAGC TACTGCCCGC GGTGTTCAGC CACAGCCAGC AGAACAGTAT CCGCAGCCTG GCTGCGCTGG CTTTGCTAGA GACCAACATC CTGGCACCTG GTCACGGCGA GCTGTGGCAC GGGCCGATTC GCAAAGCTAC CGATGAGGCC CTCGAACGAG CCCAGAAATC CAATCATGTA TTTCGG
Details for MytuD.16715.a.MB2.GE30480
HARVESTED ON: 5/11/2010
SEQUENCED ON: 10/7/2010
EXPECTED MW: 72kDa
OBSERVED MW: 75kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 38
Validated AA Sequence
MAELVQITDK VHLARGHAVN WVLVTDDTGV LLIDAGYPGD RAEVLASLNK LGYTPGDVRA IVLTHAHIDH LGSAIWFARE HSTPVYCHAE EVGHAKREYR ENASVFDVAL RSWRPRVAVW GIHLLRRGGL TGDGIPTAQP LTAEAAAGLP GQPMAIFTPG HTSGHCSYVV DGVLASGDAL ITGHPMLRHR GPQLLPAVFS HSQQNSIRSL AALALLETNI LAPGHGELWH GPIRKATDEA LERAQKSNHV FR
Validated NT Sequence
gnnctcagan ccagggtcct ggttcgatgg cggagcttgt tcagatcacc gacaaggttc acctcgctcg cggccacgcg gtcaactggg tgctagtcac cgacgacacc ggcgtgctgc tgatcgacgc cggctatccc ggcgaccgtg cggaggtgct ggcctcgctg aacaaactgg gctacacacc gggtgacgtg cgcgccatcg tgctcaccca cgcgcacatc gaccacctgg gctcggcaat ctggtttgct cgtgagcaca gcacgccggt gtactgccac gccgaggagg taggtcacgc caagcgggaa taccgggaga atgcctcggt tttcgatgtt gcgctgcgca gctggcggcc ccgtgtggcg gtctggggta ttcacttgct tcgccgcggc ggcctgaccg gcgacggcat tccgaccgcc cagccgctga ccgccgaagc ggccgccggg ctgccgggtc aaccgatggc gattttcacc ccgggacaca ccagcggtca ctgctcgtat gtcgtcgacg gtgtgctggc cagcggcgat gcgctgatca ctggtcatcc gatgttgcgt caccgcgggc cacagctact gcccgcggtg ttcagccaca gccagcagaa cagtatccgc agcctggctg cgctggcttt gctagagacc aacatcctgg cacctggtca cggcgagctg tggcacgggc cgattcgcaa agctaccgat gaggccctcg aacgagccca gaaatccaat catgtatttc ggtaataaac agcacgaaca agttctgcag ccnagcttct cgaggatccg gctgctaaca aagcccgaaa ggaanctgag ttngctgctg ccnncnnt
Expected Protein Sequence
MAHHHHHHMK IEEGKLVIWI NGDKGYNGLA EVGKKFEKDT GIKVTVEHPD KLEEKFPQVA ATGDGPDIIF WAHDRFGGYA QSGLLAEITP DKAFQDKLYP FTWDAVRYNG KLIAYPIAVE ALSLIYNKDL LPNPPKTWEE IPALDKELKA KGKSALMFNL QEPYFTWPLI AADGGYAFKY ENGKYDIKDV GVDNAGAKAG LTFLVDLIKN KHMNADTDYS IAEAAFNKGE TAMTINGPWA WSNIDTSKVN YGVTVLPTFK GQPSKPFVGV LSAGINAASP NKELAKEFLE NYLLTDEGLE AVNKDKPLGA VALKSYEEEL AKDPRIAATM ENAQKGEIMP NIPQMSAFWY AVRTAVINAA SGRQTVDEAL KDAQTNSSSN NNNNNNNNNL GIEGRISEGT LEAQTQGPGS MAELVQITDK VHLARGHAVN WVLVTDDTGV LLIDAGYPGD RAEVLASLNK LGYTPGDVRA IVLTHAHIDH LGSAIWFARE HSTPVYCHAE EVGHAKREYR ENASVFDVAL RSWRPRVAVW GIHLLRRGGL TGDGIPTAQP LTAEAAAGLP GQPMAIFTPG HTSGHCSYVV DGVLASGDAL ITGHPMLRHR GPQLLPAVFS HSQQNSIRSL AALALLETNI LAPGHGELWH GPIRKATDEA LERAQKSNHV FR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcaca tgaaaatcga agaaggtaaa ctggtaatct ggattaacgg cgataaaggc tataacggtc tcgctgaagt cggtaagaaa ttcgagaaag ataccggaat taaagtcacc gttgagcatc cggataaact ggaagagaaa ttcccacagg ttgcggcaac tggcgatggc cctgacatta tcttctgggc acacgaccgc tttggtggct acgctcaatc tggcctgttg gctgaaatca ccccggacaa agcgttccag gacaagctgt atccgtttac ctgggatgcc gtacgttaca acggcaagct gattgcttac ccgatcgctg ttgaagcgtt atcgctgatt tataacaaag atctgctgcc gaacccgcca aaaacctggg aagagatccc ggcgctggat aaagaactga aagcgaaagg taagagcgcg ctgatgttca acctgcaaga accgtacttc acctggccgc tgattgctgc tgacgggggt tatgcgttca agtatgaaaa cggcaagtac gacattaaag acgtgggcgt ggataacgct ggcgcgaaag cgggtctgac cttcctggtt gacctgatta aaaacaaaca catgaatgca gacaccgatt actccatcgc agaagctgcc tttaataaag gcgaaacagc gatgaccatc aacggcccgt gggcatggtc caacatcgac accagcaaag tgaattatgg tgtaacggta ctgccgacct tcaagggtca accatccaaa ccgttcgttg gcgtgctgag cgcaggtatt aacgccgcca gtccgaacaa agagctggca aaagagttcc tcgaaaacta tctgctgact gatgaaggtc tggaagcggt taataaagac aaaccgctgg gtgccgtagc gctgaagtct tacgaggaag agttggcgaa agatccacgt attgccgcca ctatggaaaa cgcccagaaa ggtgaaatca tgccgaacat cccgcagatg tccgctttct ggtatgccgt gcgtactgcg gtgatcaacg ccgccagcgg tcgtcagact gtcgatgaag ccctgaaaga cgcgcagact aattcgagct cgaacaacaa caacaataac aataacaaca acctcgggat cgagggaagg atttcagaag gtaccctgga agctcagacc cagggtcctg gttcgatggc ggagcttgtt cagatcaccg acaaggttca cctcgctcgc ggccacgcgg tcaactgggt gctagtcacc gacgacaccg gcgtgctgct gatcgacgcc ggctatcccg gcgaccgtgc ggaggtgctg gcctcgctga acaaactggg ctacacaccg ggtgacgtgc gcgccatcgt gctcacccac gcgcacatcg accacctggg ctcggcaatc tggtttgctc gtgagcacag cacgccggtg tactgccacg ccgaggaggt aggtcacgcc aagcgggaat accgggagaa tgcctcggtt ttcgatgttg cgctgcgcag ctggcggccc cgtgtggcgg tctggggtat tcacttgctt cgccgcggcg gcctgaccgg cgacggcatt ccgaccgccc agccgctgac cgccgaagcg gccgccgggc tgccgggtca accgatggcg attttcaccc cgggacacac cagcggtcac tgctcgtatg tcgtcgacgg tgtgctggcc agcggcgatg cgctgatcac tggtcatccg atgttgcgtc accgcgggcc acagctactg cccgcggtgt tcagccacag ccagcagaac agtatccgca gcctggctgc gctggctttg ctagagacca acatcctggc acctggtcac ggcgagctgt ggcacgggcc gattcgcaaa gctaccgatg aggccctcga acgagcccag aaatccaatc atgtatttcg gtaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa