SSGCID: Target Page

MytuD.07054.a

PROBABLE CYTOCHROME P450 130 CYP130

CENTER ID:	MytuD.07054.a
ORGANISM:	Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE:	Tuberculosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MytuD.07054.a.B1.GE39932	full length		1	402

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|83332.12.peg.856
RefSeq:	NP_215280
TubercuList:	Rv0766c
MTB Network Portal:	Rv0766c
UniProt:	P9WPP5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTVRVGDPEL VLDPYDYDFH EDPYPYYRRL RDEAPLYRNE ERNFWAVSRH HDVLQGFRDS TALSNAYGVS LDPSSRTSEA YRVMSMLAMD DPAHLRMRTL VSKGFTPRRI RELEPQVLEL ARIHLDSALQ TESFDFVAEF AGKLPMDVIS ELIGVPDTDR ARIRALADAV LHREDGVADV PPPAMAASIE LMRYYADLIA EFRRRPANNL TSALLAAELD GDRLSDQEIM AFLFLMVIAG NETTTKLLAN AVYWAAHHPG QLARVFADHS RIPMWVEETL RYDTSSQILA RTVAHDLTLY DTTIPEGEVL LLLPGSANRD DRVFDDPDDY RIGREIGCKL VSFGSGAHFC LGAHLARMEA RVALGALLRR IRNYEVDDDN VVRVHSSNVR GFAHLPISVQ AR

NT Sequence

atgaccgtcc gcgtcggtga ccccgaactg gtcctggacc cctacgacta cgactttcac gaagacccgt acccgtatta tcgtcggctg cgggacgagg ccccgctgta ccgtaacgag gaacgcaatt tctgggcggt gtcgcggcac cacgacgtgc tgcaaggctt ccgggacagc acggcgttgt cgaatgccta tggggtatcc ctggatccgt cctcacgcac ttctgaggcg taccgggtaa tgtcgatgct ggccatggac gaccccgcac atctgcggat gcgtaccttg gtgtccaagg gcttcacccc acggcggatc cgtgaactcg agccgcaggt gctcgaactt gcccgcattc acctggattc ggccctgcaa accgaaagtt tcgatttcgt agcagaattc gctggcaagc tgccgatgga tgtgatttca gagctgatag gcgtgcccga caccgaccgg gcccgcatcc gcgcgctggc cgatgcggtg ctgcaccgcg aggacggcgt ggccgatgtg cccccgccgg cgatggcggc gtcgatcgag ctgatgagat attacgctga cctgatcgcg gaattccggc ggcggcccgc gaacaatctg acgtcggcac tgctggcagc cgagctcgac ggcgaccggc tttccgacca ggaaatcatg gcgttcctgt ttctcatggt gatcgccggc aacgagacca ccaccaagct actggccaat gccgtctact gggccgccca ccaccctggc cagctggccc gcgtattcgc cgaccactcc cggattccga tgtgggtgga ggaaaccctg cgctacgaca cgtccagcca gattctggcc cgcaccgtcg cgcacgatct cacgttgtac gacaccacga tccccgaggg tgaggtgttg ctgctgctac cgggatcggc caaccgtgac gaccgggtgt tcgacgaccc ggacgactat cgcatcggcc gcgaaatcgg ctgcaaacta gtcagtttcg gcagcggtgc ccacttctgt ctgggggctc acctggcccg gatggaagcc cgggtggccc tgggcgcgct gctgcgtcgg atccgcaact acgaagtcga cgacgacaac gtcgtgcgcg tccattccag caacgtgcgc ggatttgccc atctgccgat cagcgtgcag gccaggtaa

Details for MytuD.07054.a.B1.GE39932

HARVESTED ON:	2/26/2016
SEQUENCED ON:	3/1/2016
EXPECTED MW:	46kDa
OBSERVED MW:	39kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMT VRVGDPELVL DPYDYDFHED PYPYYRRLRD EAPLYRNEER NFWAVSRHHD VLQGFRDSTA LSNAYGVSLD PSSRTSEAYR VMSMLAMDDP AHLRMRTLVS KGFTPRRIRE LEPQVLELAR IHLDSALQTE SFDFVAEFAG KLPMDVISEL IGVPDTDRAR IRALADAVLH REDGVADVPP PAMAASIELM RYYADLIAEF RRRPANNLTS ALLAAELDGD RLSDQEIMAF LFLMVIAGNE TTTKLLANAV YWAAHHPGQL ARVFADHSRI PMWVEETLRY DTSSQILART VAHDLTLYDT TIPEGEVLLL LPGSANRDDR VFDDPDDYRI GREIGCKLVS FGSGAHFCLG AHLARMEARV ALGALLRRIR NYEVDDDNVV RVHSSNVRGF A

Validated NT Sequence

tgggcaaatc cgcgcacgtt gctggaatgg acgcgcacga cgttgtcgtc gtcgacttcg tagttgcgga tccgacgcag cagcgcgccc agggccaccc gggcttccat ccgggccagg tgagccccca gacagaagtg ggcaccgctg ccgaaactga ctagtttgca gccgatttcg cggccgatgc gatagtcgtc cgggtcgtcg aacacccggt cgtcacggtt ggccgatccc ggtagcagca gcaacacctc accctcgggg atcgtggtgt cgtacaacgt gagatcgtgc gcgacggtgc gggccagaat ctggctggac gtgtcgtagc gcagggtttc ctccacccac atcggaatcc gggagtggtc ggcgaatacg cgggccagct ggccagggtg gtgggcggcc cagtagacgg cattggccag tagcttggtg gtggtctcgt tgccggcgat caccatgaga aacaggaacg ccatgatttc ctggtcggaa agccggtcgc cgtcgagctc ggctgccagc agtgccgacg tcagattgtt cgcgggccgc cgccggaatt ccgcgatcag gtcagcgtaa tatctcatca gctcgatcga cgccgccatc gccggcgggg gcacatcggc cacgccgtcc tcgcggtgca gcaccgcatc ggccagcgcg cggatgcggg cccggtcggt gtcgggcacg cctatcagct ctgaaatcac atccatcggc agcttgccag cgaattctgc tacgaaatcg aaactttcgg tttgcagggc cgaatccagg tgaatgcggg caagttcgag cacctgcggc tcgagttcac ggatccgccg tggggtgaag cccttggaca ccaaggtacg catccgcaga tgtgcggggt cgtccatggc cagcatcgac attacccggt acgcctcaga agtgcgtgag gacggatcca gggatacccc ataggcattc gacaacgccg tgctgtcccg gaagccttgc agcacgtcgt ggtgccgcga caccgcccag aaattgcgtt cctcgttacg gtacagcggg gcctcgtccc gcagccgacg ataatacggg tacgggtctt cgtgaaagtc gtagtcgtag gggtccagga ccagttcggg gtcaccgacg cggacggtca tatggtggtg gtggtggtga gccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaccg tccgcgtcgg tgaccccgaa ctggtcctgg acccctacga ctacgacttt cacgaagacc cgtacccgta ttatcgtcgg ctgcgggacg aggccccgct gtaccgtaac gaggaacgca atttctgggc ggtgtcgcgg caccacgacg tgctgcaagg cttccgggac agcacggcgt tgtcgaatgc ctatggggta tccctggatc cgtcctcacg cacttctgag gcgtaccggg taatgtcgat gctggccatg gacgaccccg cacatctgcg gatgcgtacc ttggtgtcca agggcttcac cccacggcgg atccgtgaac tcgagccgca ggtgctcgaa cttgcccgca ttcacctgga ttcggccctg caaaccgaaa gtttcgattt cgtagcagaa ttcgctggca agctgccgat ggatgtgatt tcagagctga taggcgtgcc cgacaccgac cgggcccgca tccgcgcgct ggccgatgcg gtgctgcacc gcgaggacgg cgtggccgat gtgcccccgc cggcgatggc ggcgtcgatc gagctgatga gatattacgc tgacctgatc gcggaattcc ggcggcggcc cgcgaacaat ctgacgtcgg cactgctggc agccgagctc gacggcgacc ggctttccga ccaggaaatc atggcgttcc tgtttctcat ggtgatcgcc ggcaacgaga ccaccaccaa gctactggcc aatgccgtct actgggccgc ccaccaccct ggccagctgg cccgcgtatt cgccgaccac tcccggattc cgatgtgggt ggaggaaacc ctgcgctacg acacgtccag ccagattctg gcccgcaccg tcgcgcacga tctcacgttg tacgacacca cgatccccga gggtgaggtg ttgctgctgc taccgggatc ggccaaccgt gacgaccggg tgttcgacga cccggacgac tatcgcatcg gccgcgaaat cggctgcaaa ctagtcagtt tcggcagcgg tgcccacttc tgtctggggg ctcacctggc ccggatggaa gcccgggtgg ccctgggcgc gctgctgcgt cggatccgca actacgaagt cgacgacgac aacgtcgtgc gcgtccattc cagcaacgtg cgcggatttg cccatctgcc gatcagcgtg caggccaggt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat