MytuD.01379.b

Probable 3-hydroxyisobutyrate dehydrogenase (HIBADH) (EC 1.1.1.31) (Rv0751c)

CENTER ID: MytuD.01379.b
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.01379.b.AE1.GE44469 full length 1 294
MytuD.01379.b.B1.GE44472 full length 1 294
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.840
TubercuList: Rv0751c
MTB Network Portal: Rv0751c
UniProt: P9WNY5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTTIAFLGLG NMGAPMSANL VGAGHVVRGF DPAPTAASGA AAHGVAVFRS APEAVAEADV VITMLPTGEV VRRCYTDVLA AARPATLFID SSTISVTDAR EVHALAESHG MLQLDAPVSG GVKGAAAATL AFMVGGDEST LRRARPVLEP MAGKIIHCGA AGAGQAAKVC NNMVLAVQQI AIAEAFVLAE KLGLSAQSLF DVITGATGNC WAVHTNCPVP GPVPTSPANN DFKPGFSTAL MNKDLGLAMD AVAATGATAP LGSHAADIYA KFAADHADLD FSAVIHTLRA RADA
NT Sequence
atgacgacca tcgccttcct aggtttgggc aacatgggtg cgccgatgtc ggcgaatctg gttggtgcgg gccacgtcgt gcgtggattc gacccggcac ccacggcggc gtccggcgcc gccgcgcacg gtgtcgcggt gtttcgtagc gcgcccgaag cggtggccga ggccgacgtg gtcatcacca tgctgcccac cggcgaggtg gtccggcgct gctacaccga cgtgctggcc gccgcgcgtc cggcaacgct gttcatcgac agctccacga tctcggtcac cgatgcccgt gaggtgcacg cgctggccga atcgcacggc atgctccaac tggatgcgcc ggtctccggc ggggtgaagg gcgccgccgc cgcgacgctg gcattcatgg tcggcggcga cgagtccacg ctacggcggg cacgcccggt actagagccc atggcgggca agatcattca ctgcggcgcc gccggtgccg gacaggccgc caaggtgtgc aacaacatgg tgctggcggt gcagcagatc gcgatcgccg aggcgttcgt gctggccgag aagctcgggc tgtccgcaca atcgttgttc gacgtcatca ccggcgcgac cggcaattgc tgggcggtgc acaccaattg cccggtgccg ggcccggtgc ccacctcacc ggccaacaac gacttcaagc ccgggttttc gaccgcgttg atgaacaagg acctgggcct ggcgatggat gcggtggccg ccaccggtgc gacggccccg ctgggcagcc acgccgccga catctacgcc aaattcgccg ccgaccacgc cgacctggac ttcagcgcgg tgatccacac gttgcgcgcg cgagcagacg cataa
Details for MytuD.01379.b.AE1.GE44469
HARVESTED ON: 6/30/2021
SEQUENCED ON: 7/22/2021
EXPECTED MW: 31kDa
OBSERVED MW: 31kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MTTIAFLGLG NMGAPMSANL VGAGHVVRGF DPAPTAASGA AAHGVAVFRS APEAVAEADV VITMLPTGEV VRRCYTDVLA AARPATLFID SSTISVTDAR EVHALAESHG MLQLDAPVSG GVKGAAAATL AFMVGGDEST LRRARPVLEP MAGKIIHCGA AGAGQAAKVC NNMVLAVQQI AIAEAFVLAE KLGLSAQSLF DVITGATGNC WAVHTNCPVP GPVPTSPANN DFKPGFSTAL MNKDLGLAMD AVAATGATAP LGSHAADIYA KFAADHADLD FSAVIHTLRA RADAAGHHHH HH
Validated NT Sequence
tccgttaatg atgatgatgg tggtgcccag ctgcgtctgc tcgcgcgcgc aacgtgtgga tcaccgcgct gaagtccagg tcggcgtggt cggcggcgaa tttggcgtag atgtcggcgg cgtggctgcc cagcggggcc gtcgcaccgg tggcggccac cgcatccatc gccaggccca ggtccttgtt catcaacgcg gtcgaaaacc cgggcttgaa gtcgttgttg gccggtgagg tgggcaccgg gcccggcacc gggcaattgg tgtgcaccgc ccagcaattg ccggtcgcgc cggtgatgac gtcgaacaac gattgtgcgg acagcccgag cttctcggcc agcacgaacg cctcggcgat cgcgatctgc tgcaccgcca gcaccatgtt gttgcacacc ttggcggcct gtccggcacc ggcggcgccg cagtgaatga tcttgcccgc catgggctct agtaccgggc gtgcccgccg tagcgtggac tcgtcgccgc cgaccatgaa tgccagcgtc gcggcggcgg cgcccttcac cccgccggag accggcgcat ccagttggag catgccgtgc gattcggcca gcgcgtgcac ctcacgggca tcggtgaccg agatcgtgga gctgtcgatg aacagcgttg ccggacgcgc ggcggccagc acgtcggtgt agcagcgccg gaccacctcg ccggtgggca gcatggtgat gaccacgtcg gcctcggcca ccgcttcggg cgcgctacga aacaccgcga caccgtgcgc ggcggcgccg gacgccgccg tgggtgccgg gtcgaatcca cgcacgacgt ggcccgcacc aaccagattc gccgacatcg gcgcacccat gttgcccaaa cctaggaagg cgatggtcgt catgggagag a
Expected Protein Sequence
MTTIAFLGLG NMGAPMSANL VGAGHVVRGF DPAPTAASGA AAHGVAVFRS APEAVAEADV VITMLPTGEV VRRCYTDVLA AARPATLFID SSTISVTDAR EVHALAESHG MLQLDAPVSG GVKGAAAATL AFMVGGDEST LRRARPVLEP MAGKIIHCGA AGAGQAAKVC NNMVLAVQQI AIAEAFVLAE KLGLSAQSLF DVITGATGNC WAVHTNCPVP GPVPTSPANN DFKPGFSTAL MNKDLGLAMD AVAATGATAP LGSHAADIYA KFAADHADLD FSAVIHTLRA RADAGHHHHH H
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atttgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat cgatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt taagaaggag tctctcccat gacgaccatc gccttcctag gtttgggcaa catgggtgcg ccgatgtcgg cgaatctggt tggtgcgggc cacgtcgtgc gtggattcga cccggcaccc acggcggcgt ccggcgccgc cgcgcacggt gtcgcggtgt ttcgtagcgc gcccgaagcg gtggccgagg ccgacgtggt catcaccatg ctgcccaccg gcgaggtggt ccggcgctgc tacaccgacg tgctggccgc cgcgcgtccg gcaacgctgt tcatcgacag ctccacgatc tcggtcaccg atgcccgtga ggtgcacgcg ctggccgaat cgcacggcat gctccaactg gatgcgccgg tctccggcgg ggtgaagggc gccgccgccg cgacgctggc attcatggtc ggcggcgacg agtccacgct acggcgggca cgcccggtac tagagcccat ggcgggcaag atcattcact gcggcgccgc cggtgccgga caggccgcca aggtgtgcaa caacatggtg ctggcggtgc agcagatcgc gatcgccgag gcgttcgtgc tggccgagaa gctcgggctg tccgcacaat cgttgttcga cgtcatcacc ggcgcgaccg gcaattgctg ggcggtgcac accaattgcc cggtgccggg cccggtgccc acctcaccgg ccaacaacga cttcaagccc gggttttcga ccgcgttgat gaacaaggac ctgggcctgg cgatggatgc ggtggccgcc accggtgcga cggccccgct gggcagccac gccgccgaca tctacgccaa attcgccgcc gaccacgccg acctggactt cagcgcggtg atccacacgt tgcgcgcgcg agcagacgca gggcaccacc atcatcatca ttaacggatc cgaattcgag ctccgtcgac aagcttgcgg ccgcactcga gcaccaccac caccaccact gagatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggat
Details for MytuD.01379.b.B1.GE44472
HARVESTED ON: 6/30/2021
SEQUENCED ON: 7/22/2021
EXPECTED MW: 31kDa
OBSERVED MW: 31kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMT TIAFXGLGNM GAPMSANLVG AGHVVRGFDP APTAASGAAA HGVAVFRSAP EAVAEADVVI TMLPTGEVVR RCYTDVLAAA RPATLFIDSS TISVTDAREV HALAESHGML QLDAPVSGGV KGAAAATLAF MVGGDESTLR RARPVLEPMA GKIIHCGAAG AGQAAKVCNN MVLAVQQIAI AEAFVLAEKL GLSAQSLFDV ITGATGNCWA VHTNCPVPGP VPTSPANNDF KPGFSTALMN KDLGLAMDAV AATGATAPLG SHAADIYAKF AADHADLDFS AVIHXXRARA DA
Validated NT Sequence
tactcactta tgcgtctgct cgcgcgcgcn nnntgtggat caccgcgctg aagtccaggt cggcgtggtc ggcggcgaat ttggcgtaga tgtcggcggc gtggctgccc agcggggccg tcgcaccggt ggcggccacc gcatccatcg ccaggcccag gtccttgttc atcaacgcgg tcgaaaaccc gggcttgaag tcgttgttgg ccggtgaggt gggcaccggg cccggcaccg ggcaattggt gtgcaccgcc cagcaattgc cggtcgcgcc ggtgatgacg tcgaacaacg attgtgcgga cagcccgagc ttctcggcca gcacgaacgc ctcggcgatc gcgatctgct gcaccgccag caccatgttg ttgcacacct tggcggcctg tccggcaccg gcggcgccgc agtgaatgat cttgcccgcc atgggctcta gtaccgggcg tgcccgccgt agcgtggact cgtcgccgcc gaccatgaat gccagcgtcg cggcggcggc gcccttcacc ccgccggaga ccggcgcatc cagttggagc atgccgtgcg attcggccag cgcgtgcacc tcacgggcat cggtgaccga gatcgtggag ctgtcgatga acagcgttgc cggacgcgcg gcggccagca cgtcggtgta gcagcgccgg accacctcgc cggtgggcag catggtgatg accacgtcgg cctcggccac cgcttcgggc gcgctacgaa acaccgcgac accgtgcgcg gcggcgccgg acgccgccgt gggtgccggg tcgaatccac gcacgacgtg gcccgcacca accagattcg ccgacatcgg cgcacccatg ttgcccaaac ctnggaaggc gatggtcgtc atatggtggt ggtggtggtg agccat
Expected Protein Sequence
MAHHHHHHMT TIAFLGLGNM GAPMSANLVG AGHVVRGFDP APTAASGAAA HGVAVFRSAP EAVAEADVVI TMLPTGEVVR RCYTDVLAAA RPATLFIDSS TISVTDAREV HALAESHGML QLDAPVSGGV KGAAAATLAF MVGGDESTLR RARPVLEPMA GKIIHCGAAG AGQAAKVCNN MVLAVQQIAI AEAFVLAEKL GLSAQSLFDV ITGATGNCWA VHTNCPVPGP VPTSPANNDF KPGFSTALMN KDLGLAMDAV AATGATAPLG SHAADIYAKF AADHADLDFS AVIHTLRARA DA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacga ccatcgcctt cctaggtttg ggcaacatgg gtgcgccgat gtcggcgaat ctggttggtg cgggccacgt cgtgcgtgga ttcgacccgg cacccacggc ggcgtccggc gccgccgcgc acggtgtcgc ggtgtttcgt agcgcgcccg aagcggtggc cgaggccgac gtggtcatca ccatgctgcc caccggcgag gtggtccggc gctgctacac cgacgtgctg gccgccgcgc gtccggcaac gctgttcatc gacagctcca cgatctcggt caccgatgcc cgtgaggtgc acgcgctggc cgaatcgcac ggcatgctcc aactggatgc gccggtctcc ggcggggtga agggcgccgc cgccgcgacg ctggcattca tggtcggcgg cgacgagtcc acgctacggc gggcacgccc ggtactagag cccatggcgg gcaagatcat tcactgcggc gccgccggtg ccggacaggc cgccaaggtg tgcaacaaca tggtgctggc ggtgcagcag atcgcgatcg ccgaggcgtt cgtgctggcc gagaagctcg ggctgtccgc acaatcgttg ttcgacgtca tcaccggcgc gaccggcaat tgctgggcgg tgcacaccaa ttgcccggtg ccgggcccgg tgcccacctc accggccaac aacgacttca agcccgggtt ttcgaccgcg ttgatgaaca aggacctggg cctggcgatg gatgcggtgg ccgccaccgg tgcgacggcc ccgctgggca gccacgccgc cgacatctac gccaaattcg ccgccgacca cgccgacctg gacttcagcg cggtgatcca cacgttgcgc gcgcgagcag acgcatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat