SSGCID: Target Page

MytuD.01263.b

probable acyl-CoA dehydrogenase fadE28 (Rv3544c)

CENTER ID:	MytuD.01263.b
ORGANISM:	Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE:	Tuberculosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MytuD.01263.b.A1.GE30694	full length		1	339

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|83332.12.peg.3958
RefSeq:	NP_218061.1
TubercuList:	Rv3544c
MTB Network Portal:	Rv3544c
UniProt:	P71857

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDFDPTAEQQ AVADVVTSVL ERDISWEALV CGGVTALPVP ERLGGDGVGL FEVGALLTEV GRHGAVTPAL ATLGLGVVPL LELASAEQQD RFLAGVAKGG VLTAALNEPG AALPDRPATS FVGGRLSGTK VGVGYAEQAD WMLVTADNAV VVVSPTADGV RMVRTPTSNG SDEYVMTMDG VAVADCDILA DVAAHRVNQL ALAVMGAYAD GLVAGALRLT ADYVANRKQF GKPLSTFQTV AAQLAEVYIA SRTIDLVAKS VIWRLAEDLD AGDDLGVLGY WVTSQAPPAM QICHHLHGGM GMDVTYPMHR YYSTIKDLTR LLGGPSHRLE LLGARCSLT

NT Sequence

atggatttcg atcccactgc ggaacagcaa gccgtcgccg acgtggtcac gtcggtgttg gagcgggaca tctcgtggga ggcgctggtc tgcggtggtg tgacggcgct gccggtgccg gaacgtctcg gcggcgacgg cgtgggccta ttcgaagtcg gcgcgctgct caccgaggtg ggtcgccacg gcgccgtcac gccggcgctg gcaacgctgg gtcttggggt ggtgccgctg ctcgagctgg catccgccga gcaacaggac cgcttcctgg ccggggtggc caagggagga gtactgaccg ccgcgctcaa cgagcccggg gcggcgctgc cggatcggcc tgccactagc tttgtgggtg ggcggttgtc gggcaccaaa gtcggtgttg gctacgccga acaggcggac tggatgttgg tgaccgccga caacgccgtc gtggtggtgt cgccgacggc cgacggtgtg cgcatggttc gtacgcccac atcgaatggt tcagacgagt atgtgatgac gatggacggc gtggcggttg ccgactgtga catcctggcg gatgtagccg cgcaccgggt caatcagcta gcgctggccg tcatgggtgc atacgccgac gggctggtcg ccggggcgct gcggctgacc gccgactacg tggcgaaccg gaagcagttc ggtaagccgc tgtcaacctt ccagaccgtg gccgcgcagc tcgccgaggt ttacatcgcc tcgcgcacca ttgacctggt ggccaagtcg gtgatctgga gactggccga ggatctggat gcgggagatg atctgggcgt cctcgggtac tgggtgacat cgcaggcgcc gccggcgatg caaatctgcc atcacctgca cggcggcatg gggatggacg tcacgtatcc gatgcaccgc tactactcca cgatcaagga cctcacccgg ctgctgggcg ggccctcgca tcgccttgag ctcctgggag cgcgatgttc attgacctga

Details for MytuD.01263.b.A1.GE30694

HARVESTED ON:	11/9/2010
SEQUENCED ON:	1/13/2011
EXPECTED MW:	37kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	93

Validated AA Sequence

Validated NT Sequence

ggtcaatgaa catcgcgctc ccaggagctc aaggcgatgc gagggcccgc ccagcagccg ggtgaggtcc ttgatcgtgg agtagtagcg gtgcatcgga tacgtgacgt ccatccccat gccgccgtgc aggtgatggc agatttgcat cgccggcggc gcctgcgatg tcacccagta cccgaggacg cccagatcat ctcccgcatc cagatcctcg gccagtctcc agatcaccga cttggccacc aggtcaatgg tgcgcgaggc gatgtaaacc tcggcgagct gcgcggccac ggtctggaag gttgacagcg gcttaccgaa ctgcttccgg ttcgccacgt agtcggcggt cagccgcagc gccccggcga ccagcccgtc ggcgtatgca cccatgacgg ccagcgctag ctgattgacc cggtgcgcgg ctacatccgc caggatgtca cagtcggcaa ccgccacgcc gtccatcgtc atcacatact cgtctgaacc attcgatgtg ggcgtacgaa ccatgcgcac accgtcggcc gtcggcgaca ccaccacgac ggcgttgtcg gcggtcacca acatccagtc cgcctgttcg gcgtagccaa caccgacttt ggtgcccgac aaccgcccac ccacaaagct agtggcaggc cgatccggca gcgccgcccc gggctcgttg agcgcggcgg tcagtactcc tcccttggcc accccggcca ggaagcggtc ctgttgctcg gcggatgcca gctcgagcag cggcaccacc ccaagaccca gcgttgccag cgccggcgtg acggcgccgt ggcgacccac ctcggtgagc agcgcgccga cttcgaatag gcccacgccg tcgccgccga gacgttccgg caccggcagc gccgtcacac caccgcagac cagcgcctcc cacgagatgt cccgctccaa caccgacgtg accacgtcgg cgacggcttg ctgttccgca gtgggatcga aatccatcga accaggaccc tgggtctgag cttccagggt accc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMDFDPTAEQ QAVADVVTSV LERDISWEAL VCGGVTALPV PERLGGDGVG LFEVGALLTE VGRHGAVTPA LATLGLGVVP LLELASAEQQ DRFLAGVAKG GVLTAALNEP GAALPDRPAT SFVGGRLSGT KVGVGYAEQA DWMLVTADNA VVVVSPTADG VRMVRTPTSN GSDEYVMTMD GVAVADCDIL ADVAAHRVNQ LALAVMGAYA DGLVAGALRL TADYVANRKQ FGKPLSTFQT VAAQLAEVYI ASRTIDLVAK SVIWRLAEDL DAGDDLGVLG YWVTSQAPPA MQICHHLHGG MGMDVTYPMH RYYSTIKDLT RLLGGPSHRL ELLGARCSLT

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggatttcgat cccactgcgg aacagcaagc cgtcgccgac gtggtcacgt cggtgttgga gcgggacatc tcgtgggagg cgctggtctg cggtggtgtg acggcgctgc cggtgccgga acgtctcggc ggcgacggcg tgggcctatt cgaagtcggc gcgctgctca ccgaggtggg tcgccacggc gccgtcacgc cggcgctggc aacgctgggt cttggggtgg tgccgctgct cgagctggca tccgccgagc aacaggaccg cttcctggcc ggggtggcca agggaggagt actgaccgcc gcgctcaacg agcccggggc ggcgctgccg gatcggcctg ccactagctt tgtgggtggg cggttgtcgg gcaccaaagt cggtgttggc tacgccgaac aggcggactg gatgttggtg accgccgaca acgccgtcgt ggtggtgtcg ccgacggccg acggtgtgcg catggttcgt acgcccacat cgaatggttc agacgagtat gtgatgacga tggacggcgt ggcggttgcc gactgtgaca tcctggcgga tgtagccgcg caccgggtca atcagctagc gctggccgtc atgggtgcat acgccgacgg gctggtcgcc ggggcgctgc ggctgaccgc cgactacgtg gcgaaccgga agcagttcgg taagccgctg tcaaccttcc agaccgtggc cgcgcagctc gccgaggttt acatcgcctc gcgcaccatt gacctggtgg ccaagtcggt gatctggaga ctggccgagg atctggatgc gggagatgat ctgggcgtcc tcgggtactg ggtgacatcg caggcgccgc cggcgatgca aatctgccat cacctgcacg gcggcatggg gatggacgtc acgtatccga tgcaccgcta ctactccacg atcaaggacc tcacccggct gctgggcggg ccctcgcatc gccttgagct cctgggagcg cgatgttcat tgacctaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa