MytuD.01097.b

Uncharacterized oxidoreductase (Rv1543)

CENTER ID: MytuD.01097.b
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.01097.b.B1.GE43501 full length 1 341
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.1723
TubercuList: Rv1543
MTB Network Portal: Rv1543
UniProt: P9WGS1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNLGDLTNFV EKPLAAVSNI VNTPNSAGRY RPFYLRNLLD AVQGRNLNDA VKGKVVLITG GSSGIGAAAA KKIAEAGGTV VLVARTLENL ENVANDIRAI RGNGGTAHVY PCDLSDMDAI AVMADQVLGD LGGVDILINN AGRSIRRSLE LSYDRIHDYQ RTMQLNYLGA VQLILKFIPG MRERHFGHIV NVSSVGVQTR APRFGAYIAS KAALDSLCDA LQAETVHDNV RFTTVHMALV RTPMISPTTI YDKFPTLTPD QAAGVITDAI VHRPRRASSP FGQFAAVADA VNPAVMDRVR NRAFNMFGDS SAAKGSESQT DTSELDKRSE TFVRATRGIH W
NT Sequence
atgaatcttg gtgacttaac gaacttcgtc gagaagccgc tcgcggcggt gtccaacatc gtcaacaccc cgaactcggc cgggcgatat cggcccttct acttgcgcaa cttgctcgat gcggtgcagg gccgcaacct caatgatgct gtcaagggca aggttgtcct catcactggt gggtcatcag gcatcggtgc ggcggccgcg aagaaaattg ccgaggccgg cggcacggtg gtgttggtcg cacgcaccct ggaaaacctc gagaacgtcg ccaacgacat acgggcgatc cgaggcaacg gtgggaccgc ccacgtctac ccgtgcgatc tatccgacat ggatgcgatt gccgtgatgg ccgaccaggt gctcggcgac ctcggcggcg tcgacatctt gatcaacaac gcgggccggt caattcggcg ctcgttggag ttgtcctatg accggatcca cgattaccag cgaacgatgc agctcaacta cctcggcgcg gtccagctga tcctgaagtt catccccgga atgcgagaac gccacttcgg gcatatcgtc aacgtttcct cagtcggcgt gcagacccgc gcgccgcgct tcggcgctta catcgccagc aaggccgcgc tggacagcct gtgtgatgcg ttgcaagccg agaccgtgca cgacaacgtc cgattcacca ccgtgcacat ggcattggta aggactccaa tgatcagccc gaccacgatc tacgacaagt ttcccacgct gacgccggat caggcggccg gtgtgatcac cgatgccatc gtgcatcggc cccggcgagc cagctcaccg ttcggacagt tcgccgccgt tgccgacgcc gtcaaccccg cggtgatgga ccgggtacgt aaccgtgcct tcaacatgtt cggcgactcg tccgcagcca agggaagtga atcccaaacc gacacatcag aactcgacaa gcgaagcgag acgtttgtgc gggccacccg agggatccat tggtga
Details for MytuD.01097.b.B1.GE43501
HARVESTED ON: 11/19/2019
SEQUENCED ON: 12/3/2019
EXPECTED MW: 38kDa
OBSERVED MW: 38kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMN LGDLTNFVEK PLAAVSNIVN TPNSAGRYRP FYLRNLLDAV QGRNLNDAVK GKVVLITGGS SGIGAAAAKK IAEAGGTVVL VARTLENLEN VANDIRAIRG NGGTAHVYPC DLSDMDAIAV MADQVLGDLG GVDILINNAG RSIRRSLELS YDRIHDYQRT MQLNYLGAVQ LILKFIPGMR ERHFGHIVNV SSVGVQTRAP RFGAYIASKA ALDSLCDALQ AETVHDNVRF TTVHMALVRT PMISPATIYD KFPTLTPDQA AGVITDAIVH RPRRASSPFG QFAAVADAVN PAVMDRVRNR AFNMFGDSSA AKGSESQTDT SELDKRSETF VRATRGIHW
Validated NT Sequence
ttaccaatgg atccctcggg tggcccgcac aaacgtctcg cttcgcttgt cgagttctga tgtgtcggtt tgggattcac ttcccttggc tgcggacgag tcgccgaaca tgttgaaggc acggttacgt acccggtcca tcaccgcggg gttgacggcg tcggcaacgg cggcgaactg tccgaacggt gagctggctc gccggggccg atgcacgatg gcatcggtga tcacaccggc cgcctgatcc ggcgtcagcg tgggaaactt gtcgtagatc gtggccgggc tgatcattgg agtccttacc aatgccatgt gcacggtggt gaatcggacg ttgtcgtgca cggtctcggc ttgcaacgca tcacacaggc tgtccagcgc ggccttgctg gcgatgtaag cgccgaagcg cggcgcgcgg gtctgcacgc cgactgagga aacgttgacg atatgcccga agtggcgttc tcgcattccg gggatgaact tcaggatcag ctggaccgcg ccgaggtagt tgagctgcat cgttcgctgg taatcgtgga tccggtcata ggacaactcc aacgagcgcc gaattgaccg gcccgcgttg ttgatcaaga tgtcgacgcc gccgaggtcg ccgagcacct ggtcggccat cacggcaatc gcatccatgt cggatagatc gcacgggtag acgtgggcgg tcccaccgtt gcctcggatc gcccgtatgt cgttggcgac gttctcgagg ttttccaggg tgcgtgcgac caacaccacc gtgccgccgg cctcggcaat tttcttcgcg gccgccgcac cgatgcctga tgacccacca gtgatgagga caaccttgcc cttgacagca tcattgaggt tgcggccctg caccgcatcg agcaagttgc gcaagtagaa gggccgatat cgcccggccg agttcggggt gttgacgatg ttggacaccg ccgcgagcgg cttctcgacg aagttcgtta agtcaccaag attcatatgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHMN LGDLTNFVEK PLAAVSNIVN TPNSAGRYRP FYLRNLLDAV QGRNLNDAVK GKVVLITGGS SGIGAAAAKK IAEAGGTVVL VARTLENLEN VANDIRAIRG NGGTAHVYPC DLSDMDAIAV MADQVLGDLG GVDILINNAG RSIRRSLELS YDRIHDYQRT MQLNYLGAVQ LILKFIPGMR ERHFGHIVNV SSVGVQTRAP RFGAYIASKA ALDSLCDALQ AETVHDNVRF TTVHMALVRT PMISPTTIYD KFPTLTPDQA AGVITDAIVH RPRRASSPFG QFAAVADAVN PAVMDRVRNR AFNMFGDSSA AKGSESQTDT SELDKRSETF VRATRGIHW
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaatc ttggtgactt aacgaacttc gtcgagaagc cgctcgcggc ggtgtccaac atcgtcaaca ccccgaactc ggccgggcga tatcggccct tctacttgcg caacttgctc gatgcggtgc agggccgcaa cctcaatgat gctgtcaagg gcaaggttgt cctcatcact ggtgggtcat caggcatcgg tgcggcggcc gcgaagaaaa ttgccgaggc cggcggcacg gtggtgttgg tcgcacgcac cctggaaaac ctcgagaacg tcgccaacga catacgggcg atccgaggca acggtgggac cgcccacgtc tacccgtgcg atctatccga catggatgcg attgccgtga tggccgacca ggtgctcggc gacctcggcg gcgtcgacat cttgatcaac aacgcgggcc ggtcaattcg gcgctcgttg gagttgtcct atgaccggat ccacgattac cagcgaacga tgcagctcaa ctacctcggc gcggtccagc tgatcctgaa gttcatcccc ggaatgcgag aacgccactt cgggcatatc gtcaacgttt cctcagtcgg cgtgcagacc cgcgcgccgc gcttcggcgc ttacatcgcc agcaaggccg cgctggacag cctgtgtgat gcgttgcaag ccgagaccgt gcacgacaac gtccgattca ccaccgtgca catggcattg gtaaggactc caatgatcag cccgaccacg atctacgaca agtttcccac gctgacgccg gatcaggcgg ccggtgtgat caccgatgcc atcgtgcatc ggccccggcg agccagctca ccgttcggac agttcgccgc cgttgccgac gccgtcaacc ccgcggtgat ggaccgggta cgtaaccgtg ccttcaacat gttcggcgac tcgtccgcag ccaagggaag tgaatcccaa accgacacat cagaactcga caagcgaagc gagacgtttg tgcgggccac ccgagggatc cattggtgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t