MytuD.01097.a

PROBABLE SHORT-CHAIN TYPE DEHYDROGENASE/REDUCTASE

CENTER ID: MytuD.01097.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.01097.a.A1.GE25936 Full length( MytuD.01097.a ) 1 276
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.01097.a.A1.PS00177 Full length( MytuD.01097.a ) 1 276

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.1394
RefSeq: NP_215761.1
TubercuList: Rv1245c
MTB Network Portal: Rv1245c
UniProt: O50460

Orthologues for Rv1245c

Species % Identity % Coverage Clones Proteins Structures
paratuberculosis 85 99 1 0 0
smegmatis 83 99 1 0 0
marinum 82 99 1 1 0
thermoresistibile 82 99 1 1 0
ulcerans 81 99 1 0 0
leprae 79 100 0 0 0
paratuberculosis 74 99 1 0 0
abscessus 71 100 0 1 0
abscessus 68 99 1 0 0
smegmatis 39 91 1 0 0
thermoresistibile 37 90 1 0 0
abscessus 32 93 1 1 0
avium 32 86 1 1 0
paratuberculosis 32 86 1 1 0
abscessus 31 89 0 0 0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEGFAGKVAV VTGAGSGIGQ ALAIELARSG AKVAISDVDT DGLADTEHRL KAISTPVKTD RLDVTEREAF LAYADAVNEH FGTVNQIYNN AGIAFTGDIE VSQFKDIERV MDVDFWGVVN GTKAFLPHLI ASGDGHVINI SSVFGLFSAP GQAAYNSAKF AVRGFTEALR QEMALAGHPV KVTTVHPGGV KTAIARNATA AEGLDQAELA ETFDKRVAHL SPQRAAQIIL TGVAKNKARV LVGVDAKVLD LVVRLTGSGY QRIFPIITGR LIPRPR
NT Sequence
ATGGAGGGGT TCGCCGGAAA GGTCGCCGTT GTCACCGGCG CGGGATCGGG CATCGGGCAG GCGCTGGCAA TCGAGCTGGC CCGCTCGGGC GCCAAGGTGG CCATCAGCGA CGTCGACACC GACGGCCTGG CAGACACCGA GCACCGGCTA AAGGCGATCA GCACGCCGGT CAAGACGGAC CGACTCGACG TAACCGAACG CGAAGCCTTC CTAGCCTACG CCGATGCGGT CAACGAGCAC TTCGGCACTG TGAACCAGAT CTACAACAAC GCCGGCATCG CCTTTACCGG CGATATCGAG GTCAGCCAGT TCAAGGACAT CGAACGGGTG ATGGACGTCG ATTTTTGGGG TGTGGTCAAC GGCACCAAGG CGTTCCTGCC GCATTTGATC GCCTCCGGGG ACGGCCACGT CATCAACATC TCCAGCGTGT TCGGGTTGTT TTCGGCGCCG GGTCAGGCGG CCTACAACTC GGCCAAGTTC GCCGTCCGCG GCTTTACCGA GGCGCTGCGC CAGGAAATGG CGTTGGCGGG TCACCCGGTG AAGGTGACGA CGGTGCACCC CGGCGGTGTC AAGACCGCGA TCGCCCGTAA CGCCACCGCC GCCGAGGGAC TTGACCAGGC CGAGCTGGCC GAGACGTTCG ATAAGCGGGT GGCCCATCTC AGCCCACAGC GGGCCGCGCA GATCATCCTG ACGGGGGTGG CCAAGAACAA GGCCCGGGTG TTGGTGGGTG TCGACGCCAA GGTACTGGAC CTGGTGGTCC GGCTGACCGG TTCCGGATAT CAGCGGATCT TTCCGATCAT CACCGGCCGG TTGATACCCC GCCCGCGC
Details for MytuD.01097.a.A1.GE25936
HARVESTED ON: 1/9/2009
SEQUENCED ON: 8/18/2010
EXPECTED MW: 31kDa
OBSERVED MW: 32kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAQTQG PGSMEGFAGK VAVVTGAGSG IGQALAIELA RSGAKVAISD VDTDGLADTE HRLKAISTPV KTDRLDVTER EAFLAYADAV NEHFGTVNQI YNNAGIAFTG DIEVSQFKDI ERVMDVDFWG VVNGTKAFLP HLIASGDGHV INISSVFGLF SAPGQAAYNS AKFAVRGFTE ALRQEMALAG HPVKVTTVHP GGVKTAIARN ATAAEGLDQA ELAETFDKRV AHLSPQRAAQ IILTGVAKNK ARVLVGVDAK VLDLVVRLTG SGYQRIFPII TGRLIPRPR
Validated NT Sequence
ttattagcgc gggcggggta tcaaccggcc ggtgatgatc ggaaagatcc gctgatatcc ggaaccggtc agccggacca ccaggtccag taccttggcg tcgacaccca ccaacacccg ggccttgttc ttggccaccc ccgtcaggat gatctgcgcg gcccgctgtg ggctgagatg ggccacccgc ttatcgaacg tctcggccag ctcggcctgg tcaagtccct cggcggcggt ggcgttacgg gcgatcgcgg tcttgacacc gccggggtgc accgtcgtca ccttcaccgg gtgacccgcc aacgccattt cctggcgcag cgcctcggta aagccgcgga cggcgaactt ggccgagttg taggccgcct gacccggcgc cgaaaacaac ccgaacacgc tggagatgtt gatgacgtgg ccgtccccgg aggcgatcaa atgcggcagg aacgccttgg tgccgttgac cacaccccaa aaatcgacgt ccatcacccg ttcgatgtcc ttgaactggc tgacctcgat atcgccggta aaggcgatgc cggcgttgtt gtagatctgg ttcacagtgc cgaagtgctc gttgaccgca tcggcgtagg ctaggaaggc ttcgcgttcg gttacgtcga gtcggtccgt cttgaccggc gtgctgatcg cctttagccg gtgctcggtg tctgccaggc cgtcggtgtc gacgtcgctg atggccacct tggcgcccga gcgggccagc tcgattgcca gcgcctgccc gatgcccgat cccgcgccgg tgacaacggc gacctttccg gcgaacccct ccatcgaacc aggaccctgg gtctgagctt ccagggtacc catatgatgg tgatggtgat gagc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMEGFAGKVA VVTGAGSGIG QALAIELARS GAKVAISDVD TDGLADTEHR LKAISTPVKT DRLDVTEREA FLAYADAVNE HFGTVNQIYN NAGIAFTGDI EVSQFKDIER VMDVDFWGVV NGTKAFLPHL IASGDGHVIN ISSVFGLFSA PGQAAYNSAK FAVRGFTEAL RQEMALAGHP VKVTTVHPGG VKTAIARNAT AAEGLDQAEL AETFDKRVAH LSPQRAAQII LTGVAKNKAR VLVGVDAKVL DLVVRLTGSG YQRIFPIITG RLIPRPR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggaggggttc gccggaaagg tcgccgttgt caccggcgcg ggatcgggca tcgggcaggc gctggcaatc gagctggccc gctcgggcgc caaggtggcc atcagcgacg tcgacaccga cggcctggca gacaccgagc accggctaaa ggcgatcagc acgccggtca agacggaccg actcgacgta accgaacgcg aagccttcct agcctacgcc gatgcggtca acgagcactt cggcactgtg aaccagatct acaacaacgc cggcatcgcc tttaccggcg atatcgaggt cagccagttc aaggacatcg aacgggtgat ggacgtcgat ttttggggtg tggtcaacgg caccaaggcg ttcctgccgc atttgatcgc ctccggggac ggccacgtca tcaacatctc cagcgtgttc gggttgtttt cggcgccggg tcaggcggcc tacaactcgg ccaagttcgc cgtccgcggc tttaccgagg cgctgcgcca ggaaatggcg ttggcgggtc acccggtgaa ggtgacgacg gtgcaccccg gcggtgtcaa gaccgcgatc gcccgtaacg ccaccgccgc cgagggactt gaccaggccg agctggccga gacgttcgat aagcgggtgg cccatctcag cccacagcgg gccgcgcaga tcatcctgac gggggtggcc aagaacaagg cccgggtgtt ggtgggtgtc gacgccaagg tactggacct ggtggtccgg ctgaccggtt ccggatatca gcggatcttt ccgatcatca ccggccggtt gataccccgc ccgcgcaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for MytuD.01097.a.A1.PS00177
PURIFICATION DATe: 3/12/2009
CONCENTRATION: 8.38mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 9
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMEGFAGK VAVVTGAGSG IGQALAIELA RSGAKVAISD VDTDGLADTE HRLKAISTPV KTDRLDVTER EAFLAYADAV NEHFGTVNQI YNNAGIAFTG DIEVSQFKDI ERVMDVDFWG VVNGTKAFLP HLIASGDGHV INISSVFGLF SAPGQAAYNS AKFAVRGFTE ALRQEMALAG HPVKVTTVHP GGVKTAIARN ATAAEGLDQA ELAETFDKRV AHLSPQRAAQ IILTGVAKNK ARVLVGVDAK VLDLVVRLTG SGYQRIFPII TGRLIPRPR
Validated NT Sequence
ttattagcgc gggcggggta tcaaccggcc ggtgatgatc ggaaagatcc gctgatatcc ggaaccggtc agccggacca ccaggtccag taccttggcg tcgacaccca ccaacacccg ggccttgttc ttggccaccc ccgtcaggat gatctgcgcg gcccgctgtg ggctgagatg ggccacccgc ttatcgaacg tctcggccag ctcggcctgg tcaagtccct cggcggcggt ggcgttacgg gcgatcgcgg tcttgacacc gccggggtgc accgtcgtca ccttcaccgg gtgacccgcc aacgccattt cctggcgcag cgcctcggta aagccgcgga cggcgaactt ggccgagttg taggccgcct gacccggcgc cgaaaacaac ccgaacacgc tggagatgtt gatgacgtgg ccgtccccgg aggcgatcaa atgcggcagg aacgccttgg tgccgttgac cacaccccaa aaatcgacgt ccatcacccg ttcgatgtcc ttgaactggc tgacctcgat atcgccggta aaggcgatgc cggcgttgtt gtagatctgg ttcacagtgc cgaagtgctc gttgaccgca tcggcgtagg ctaggaaggc ttcgcgttcg gttacgtcga gtcggtccgt cttgaccggc gtgctgatcg cctttagccg gtgctcggtg tctgccaggc cgtcggtgtc gacgtcgctg atggccacct tggcgcccga gcgggccagc tcgattgcca gcgcctgccc gatgcccgat cccgcgccgg tgacaacggc gacctttccg gcgaacccct ccatcgaacc aggaccctgg gtctgagctt ccagggtacc catatgatgg tgatggtgat gagc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMEGFAGKVA VVTGAGSGIG QALAIELARS GAKVAISDVD TDGLADTEHR LKAISTPVKT DRLDVTEREA FLAYADAVNE HFGTVNQIYN NAGIAFTGDI EVSQFKDIER VMDVDFWGVV NGTKAFLPHL IASGDGHVIN ISSVFGLFSA PGQAAYNSAK FAVRGFTEAL RQEMALAGHP VKVTTVHPGG VKTAIARNAT AAEGLDQAEL AETFDKRVAH LSPQRAAQII LTGVAKNKAR VLVGVDAKVL DLVVRLTGSG YQRIFPIITG RLIPRPR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGAGGGGTTC GCCGGAAAGG TCGCCGTTGT CACCGGCGCG GGATCGGGCA TCGGGCAGGC GCTGGCAATC GAGCTGGCCC GCTCGGGCGC CAAGGTGGCC ATCAGCGACG TCGACACCGA CGGCCTGGCA GACACCGAGC ACCGGCTAAA GGCGATCAGC ACGCCGGTCA AGACGGACCG ACTCGACGTA ACCGAACGCG AAGCCTTCCT AGCCTACGCC GATGCGGTCA ACGAGCACTT CGGCACTGTG AACCAGATCT ACAACAACGC CGGCATCGCC TTTACCGGCG ATATCGAGGT CAGCCAGTTC AAGGACATCG AACGGGTGAT GGACGTCGAT TTTTGGGGTG TGGTCAACGG CACCAAGGCG TTCCTGCCGC ATTTGATCGC CTCCGGGGAC GGCCACGTCA TCAACATCTC CAGCGTGTTC GGGTTGTTTT CGGCGCCGGG TCAGGCGGCC TACAACTCGG CCAAGTTCGC CGTCCGCGGC TTTACCGAGG CGCTGCGCCA GGAAATGGCG TTGGCGGGTC ACCCGGTGAA GGTGACGACG GTGCACCCCG GCGGTGTCAA GACCGCGATC GCCCGTAACG CCACCGCCGC CGAGGGACTT GACCAGGCCG AGCTGGCCGA GACGTTCGAT AAGCGGGTGG CCCATCTCAG CCCACAGCGG GCCGCGCAGA TCATCCTGAC GGGGGTGGCC AAGAACAAGG CCCGGGTGTT GGTGGGTGTC GACGCCAAGG TACTGGACCT GGTGGTCCGG CTGACCGGTT CCGGATATCA GCGGATCTTT CCGATCATCA CCGGCCGGTT GATACCCCGC CCGCGCAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA