MytuD.01083.a
PROBABLE THIOSULFATE SULFURTRANSFERASE CYSA2 (RHODANESE-LIKE PROTEIN) (THIOSULFATE CYANIDE TRANSSULFURASE) (THIOSULFATE THIOTRANSFERASE)
| CENTER ID: | MytuD.01083.a |
| ORGANISM: | Mycobacterium tuberculosis H37Rv |
| ASSOCIATED DISEASE: | Tuberculosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MytuD.01083.a.A1.GE25934 | Full length( MytuD.01083.a ) | 1 | 277 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MytuD.01083.a.A1.PS00143 | Full length( MytuD.01083.a ) | 1 | 277 |
Structures
3HWI
DEPOSITED: 6/17/2009
DETERMINATION: XRay
CLONE: MytuD.01083.a.A1.GE25934
PROTEIN: MytuD.01083.a.A1.PS00143
Publications by SSGCID
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_127129 |
| PATRIC ID: | fig|83332.12.peg.910 |
| RefSeq: | NP_215330.1 |
| TubercuList: | Rv0815c |
| MTB Network Portal: | Rv0815c |
| UniProt: | P9WHF9 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MARCDVLVSA DWAESNLHAP KVVFVEVDED TSAYDRDHIA GAIKLDWRTD LQDPVKRDFV DAQQFSKLLS ERGIANEDTV ILYGGNNNWF AAYAYWYFKL YGHEKVKLLD GGRKKWELDG RPLSSDPVSR PVTSYTASPP DNTIRAFRDE VLAAINVKNL IDVRSPDEFS GKILAPAHLP QEQSQRPGHI PGAINVPWSR AANEDGTFKS DEELAKLYAD AGLDNSKETI AYCRIGERSS HTWFVLRELL GHQNVKNYDG SWTEYGSLVG APIELGS
NT Sequence
ATGGCACGCT GCGATGTCCT GGTCTCCGCC GACTGGGCTG AGAGCAATCT GCACGCGCCG AAGGTCGTTT TCGTCGAAGT GGACGAGGAC ACCAGTGCAT ATGACCGTGA CCATATTGCC GGCGCGATCA AGTTGGACTG GCGCACCGAC CTGCAGGATC CGGTCAAACG TGACTTCGTC GACGCCCAGC AATTCTCCAA GCTGCTGTCC GAGCGTGGCA TCGCCAACGA GGACACGGTG ATCCTGTACG GCGGCAACAA CAATTGGTTC GCCGCCTACG CGTACTGGTA TTTCAAGCTC TACGGCCATG AGAAGGTCAA GTTGCTCGAC GGCGGCCGCA AGAAGTGGGA GCTCGACGGA CGCCCGCTGT CCAGCGACCC GGTCAGCCGG CCGGTGACCT CCTACACCGC CTCCCCGCCG GATAACACGA TTCGGGCATT CCGCGACGAG GTCCTGGCGG CCATCAACGT CAAGAACCTC ATCGACGTGC GCTCTCCCGA CGAGTTCTCC GGCAAGATCC TGGCCCCCGC GCACCTGCCG CAGGAACAAA GCCAGCGGCC CGGACACATT CCTGGTGCCA TCAACGTGCC GTGGAGCAGG GCCGCCAACG AGGACGGCAC CTTCAAGTCC GATGAGGAGT TGGCCAAGCT TTACGCCGAC GCCGGCCTAG ACAACAGCAA GGAAACGATT GCCTACTGCC GAATCGGGGA ACGGTCCTCG CACACCTGGT TCGTGTTGCG GGAATTACTC GGACACCAAA ACGTCAAGAA CTACGACGGC AGTTGGACAG AATACGGCTC CCTGGTGGGC GCCCCGATCG AGTTGGGAAG C
Details for MytuD.01083.a.A1.GE25934
| HARVESTED ON: | 1/9/2009 |
| SEQUENCED ON: | 8/18/2010 |
| EXPECTED MW: | 33kDa |
| OBSERVED MW: | 34kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMARCDVLVS ADWAESNLHA PKVVFVEVDE DTSAYDRDHI AGAIKLDWRT DLQDPVKRDF VDAQQFSKLL SERGIANEDT VILYGGNNNW FAAYAYWYFK LYGHEKVKLL DGGRKKWELD GRPLSSDPVS RPVTSYTASP PDNTIRAFRD EVLAAINVKN LIDVRSPDEF SGKILAPAHL PQEQSQRPGH IPGAINVPWS RAANEDGTFK SDEELAKLYA DAGLDNSKET IAYCRIGERS SHTWFVLREL LGHQNVKNYD GSWTEYGSLV GAPIELGS
Validated NT Sequence
ttattagctt cccaactcga tcggggcgcc caccagggag ccgtattctg tccaactgcc gtcgtagttc ttgacgtttt ggtgtccgag taattcccgc aacacgaacc aggtgtgcga ggaccgttcc ccgattcggc agtaggcaat cgtttccttg ctgttgtcta ggccggcgtc ggcgtaaagc ttggccaact cctcatcgga cttgaaggtg ccgtcctcgt tggcggccct gctccacggc acgttgatgg caccaggaat gtgtccgggc cgctggcttt gttcctgcgg caggtgcgcg ggggccagga tcttgccgga gaactcgtcg ggagagcgca cgtcgatgag gttcttgacg ttgatggccg ccaggacctc gtcgcggaat gcccgaatcg tgttatccgg cggggaggcg gtgtaggagg tcaccggccg gctgaccggg tcgctggaca gcgggcgtcc gtcgagctcc cacttcttgc ggccgccgtc gagcaacttg accttctcat ggccgtagag cttgaaatac cagtacgcgt aggcggcgaa ccaattgttg ttgccgccgt acaggatcac cgtgtcctcg ttggcgatgc cacgctcgga cagcagcttg gagaattgct gggcgtcgac gaagtcacgt ttgaccggat cctgcaggtc ggtgcgccag tccaacttga tcgcgccggc aatatggtca cggtcatatg cactggtgtc ctcgtccact tcgacgaaaa cgaccttcgg cgcgtgcaga ttgctctcag cccagtcggc ggagaccagg acatcgcagc gtgccatcga accaggaccc tgggtctgag cttccagggt acccatatga tggtgatggt gatgagccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMARCDVLVS ADWAESNLHA PKVVFVEVDE DTSAYDRDHI AGAIKLDWRT DLQDPVKRDF VDAQQFSKLL SERGIANEDT VILYGGNNNW FAAYAYWYFK LYGHEKVKLL DGGRKKWELD GRPLSSDPVS RPVTSYTASP PDNTIRAFRD EVLAAINVKN LIDVRSPDEF SGKILAPAHL PQEQSQRPGH IPGAINVPWS RAANEDGTFK SDEELAKLYA DAGLDNSKET IAYCRIGERS SHTWFVLREL LGHQNVKNYD GSWTEYGSLV GAPIELGS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcacgctgc gatgtcctgg tctccgccga ctgggctgag agcaatctgc acgcgccgaa ggtcgttttc gtcgaagtgg acgaggacac cagtgcatat gaccgtgacc atattgccgg cgcgatcaag ttggactggc gcaccgacct gcaggatccg gtcaaacgtg acttcgtcga cgcccagcaa ttctccaagc tgctgtccga gcgtggcatc gccaacgagg acacggtgat cctgtacggc ggcaacaaca attggttcgc cgcctacgcg tactggtatt tcaagctcta cggccatgag aaggtcaagt tgctcgacgg cggccgcaag aagtgggagc tcgacggacg cccgctgtcc agcgacccgg tcagccggcc ggtgacctcc tacaccgcct ccccgccgga taacacgatt cgggcattcc gcgacgaggt cctggcggcc atcaacgtca agaacctcat cgacgtgcgc tctcccgacg agttctccgg caagatcctg gcccccgcgc acctgccgca ggaacaaagc cagcggcccg gacacattcc tggtgccatc aacgtgccgt ggagcagggc cgccaacgag gacggcacct tcaagtccga tgaggagttg gccaagcttt acgccgacgc cggcctagac aacagcaagg aaacgattgc ctactgccga atcggggaac ggtcctcgca cacctggttc gtgttgcggg aattactcgg acaccaaaac gtcaagaact acgacggcag ttggacagaa tacggctccc tggtgggcgc cccgatcgag ttgggaagca aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for MytuD.01083.a.A1.PS00143
| PURIFICATION DATe: | 2/5/2009 |
| CONCENTRATION: | 37.41mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 6 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMARCDVLVS ADWAESNLHA PKVVFVEVDE DTSAYDRDHI AGAIKLDWRT DLQDPVKRDF VDAQQFSKLL SERGIANEDT VILYGGNNNW FAAYAYWYFK LYGHEKVKLL DGGRKKWELD GRPLSSDPVS RPVTSYTASP PDNTIRAFRD EVLAAINVKN LIDVRSPDEF SGKILAPAHL PQEQSQRPGH IPGAINVPWS RAANEDGTFK SDEELAKLYA DAGLDNSKET IAYCRIGERS SHTWFVLREL LGHQNVKNYD GSWTEYGSLV GAPIELGS
Validated NT Sequence
ttattagctt cccaactcga tcggggcgcc caccagggag ccgtattctg tccaactgcc gtcgtagttc ttgacgtttt ggtgtccgag taattcccgc aacacgaacc aggtgtgcga ggaccgttcc ccgattcggc agtaggcaat cgtttccttg ctgttgtcta ggccggcgtc ggcgtaaagc ttggccaact cctcatcgga cttgaaggtg ccgtcctcgt tggcggccct gctccacggc acgttgatgg caccaggaat gtgtccgggc cgctggcttt gttcctgcgg caggtgcgcg ggggccagga tcttgccgga gaactcgtcg ggagagcgca cgtcgatgag gttcttgacg ttgatggccg ccaggacctc gtcgcggaat gcccgaatcg tgttatccgg cggggaggcg gtgtaggagg tcaccggccg gctgaccggg tcgctggaca gcgggcgtcc gtcgagctcc cacttcttgc ggccgccgtc gagcaacttg accttctcat ggccgtagag cttgaaatac cagtacgcgt aggcggcgaa ccaattgttg ttgccgccgt acaggatcac cgtgtcctcg ttggcgatgc cacgctcgga cagcagcttg gagaattgct gggcgtcgac gaagtcacgt ttgaccggat cctgcaggtc ggtgcgccag tccaacttga tcgcgccggc aatatggtca cggtcatatg cactggtgtc ctcgtccact tcgacgaaaa cgaccttcgg cgcgtgcaga ttgctctcag cccagtcggc ggagaccagg acatcgcagc gtgccatcga accaggaccc tgggtctgag cttccagggt acccatatga tggtgatggt gatgagccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMARCDVLVS ADWAESNLHA PKVVFVEVDE DTSAYDRDHI AGAIKLDWRT DLQDPVKRDF VDAQQFSKLL SERGIANEDT VILYGGNNNW FAAYAYWYFK LYGHEKVKLL DGGRKKWELD GRPLSSDPVS RPVTSYTASP PDNTIRAFRD EVLAAINVKN LIDVRSPDEF SGKILAPAHL PQEQSQRPGH IPGAINVPWS RAANEDGTFK SDEELAKLYA DAGLDNSKET IAYCRIGERS SHTWFVLREL LGHQNVKNYD GSWTEYGSLV GAPIELGS
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCACGCTGC GATGTCCTGG TCTCCGCCGA CTGGGCTGAG AGCAATCTGC ACGCGCCGAA GGTCGTTTTC GTCGAAGTGG ACGAGGACAC CAGTGCATAT GACCGTGACC ATATTGCCGG CGCGATCAAG TTGGACTGGC GCACCGACCT GCAGGATCCG GTCAAACGTG ACTTCGTCGA CGCCCAGCAA TTCTCCAAGC TGCTGTCCGA GCGTGGCATC GCCAACGAGG ACACGGTGAT CCTGTACGGC GGCAACAACA ATTGGTTCGC CGCCTACGCG TACTGGTATT TCAAGCTCTA CGGCCATGAG AAGGTCAAGT TGCTCGACGG CGGCCGCAAG AAGTGGGAGC TCGACGGACG CCCGCTGTCC AGCGACCCGG TCAGCCGGCC GGTGACCTCC TACACCGCCT CCCCGCCGGA TAACACGATT CGGGCATTCC GCGACGAGGT CCTGGCGGCC ATCAACGTCA AGAACCTCAT CGACGTGCGC TCTCCCGACG AGTTCTCCGG CAAGATCCTG GCCCCCGCGC ACCTGCCGCA GGAACAAAGC CAGCGGCCCG GACACATTCC TGGTGCCATC AACGTGCCGT GGAGCAGGGC CGCCAACGAG GACGGCACCT TCAAGTCCGA TGAGGAGTTG GCCAAGCTTT ACGCCGACGC CGGCCTAGAC AACAGCAAGG AAACGATTGC CTACTGCCGA ATCGGGGAAC GGTCCTCGCA CACCTGGTTC GTGTTGCGGG AATTACTCGG ACACCAAAAC GTCAAGAACT ACGACGGCAG TTGGACAGAA TACGGCTCCC TGGTGGGCGC CCCGATCGAG TTGGGAAGCA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A