MytuD.01046.a
glycine cleavage system protein H
| CENTER ID: | MytuD.01046.a |
| ORGANISM: | Mycobacterium tuberculosis H37Rv |
| ASSOCIATED DISEASE: | Tuberculosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MytuD.01046.a.A1.GE25926 | Full length( MytuD.01046.a ) | 1 | 134 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
3HGB
DEPOSITED: 5/13/2009
DETERMINATION: XRay
CLONE: MytuD.01046.a.A1.GE25926
PROTEIN: MytuD.01046.a.A1.PS00145
Publications by SSGCID
X-ray structure determination of the glycine cleavage system protein H of Mycobacterium tuberculosis using an inverse Compton synchrotron X-ray source.
Abendroth J, Analau E, Edwards TE, Gifford M, Guo W, Hewitt SN, Kelley A, Kuhn P, Loewen R, Mayer C, McCormick MS, Myler P, Napuli AJ, Rifkin J, Staker B, Stewart LJ, Zhang Y, Ruth RD
J. Struct. Funct. Genomics - 2010
volume 11, issue 1, pages 91-100
PMID: 20364333; PMCID: PMC2939714
PDB:
3HGB
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_127169 |
| PATRIC ID: | fig|83332.12.peg.2039 |
| RefSeq: | NP_216342.1 |
| TubercuList: | Rv1826 |
| MTB Network Portal: | Rv1826 |
| UniProt: | P9WN55 |
Orthologues for Rv1826
| Species | % Identity | % Coverage | Clones | Proteins | Structures |
|---|---|---|---|---|---|
| paratuberculosis | 84 | 100 | 1 | 0 | 0 |
| marinum | 81 | 100 | 0 | 0 | 0 |
| ulcerans | 81 | 100 | 1 | 0 | 0 |
| leprae | 79 | 100 | 1 | 0 | 0 |
| abscessus | 69 | 96 | 1 | 1 | 0 |
| smegmatis | 68 | 100 | 1 | 0 | 0 |
| thermoresistibile | 65 | 100 | 1 | 1 | 0 |
| marinum | 51 | 95 | 1 | 1 | 1 |
| ulcerans | 51 | 95 | 1 | 1 | 0 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSDIPSDLHY TAEHEWIRRS GDDTVRVGIT DYAQSALGDV VFVQLPVIGT AVTAGETFGE VESTKSVSDL YAPISGKVSE VNSDLDGTPQ LVNSDPYGAG WLLDIQVDSS DVAALESALT TLLDAEAYRG TLTE
NT Sequence
GTGAGCGATA TCCCGTCCGA TCTGCACTAC ACCGCCGAAC ACGAGTGGAT TCGCCGCAGT GGCGACGACA CCGTCCGGGT GGGGATCACC GACTATGCAC AGTCGGCGCT TGGCGACGTC GTTTTCGTTC AGCTACCCGT TATCGGCACC GCGGTCACCG CCGGCGAGAC CTTCGGCGAA GTGGAATCGA CGAAATCTGT GTCGGATCTC TATGCGCCCA TTTCGGGTAA GGTGTCTGAG GTCAACAGCG ATCTGGACGG CACTCCGCAA TTGGTGAATT CCGACCCCTA CGGAGCCGGC TGGCTGCTGG ACATCCAGGT CGACAGCTCG GATGTCGCTG CCCTGGAGTC AGCTTTGACG ACACTGCTCG ACGCTGAGGC CTACCGCGGC ACACTGACCG AG
Details for MytuD.01046.a.A1.GE25926
| HARVESTED ON: | 1/9/2009 |
| SEQUENCED ON: | 8/18/2010 |
| EXPECTED MW: | 16kDa |
| OBSERVED MW: | 17kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVSDIPSDL HYTAEHEWIR RSGDDTVRVG ITDYAQSALG DVVFVQLPVI GTAVTAGETF GEVESTKSVS DLYAPISGKV SEVNSDLDGT PQLVNSDPYG AGWLLDIQVD SSDVAALESA LTTLLDAEAY RGTLTE
Validated NT Sequence
ttattactcg gtcagtgtgc cgcggtaggc ctcagcgtcg agcagtgtcg tcaaagctga ctccagggca gcgacatccg agctgtcgac ctggatgtcc agcagccagc cggctccgta ggggtcggaa ttcaccaatt gcggagtgcc gtccagatcg ctgttgacct cagacacctt acccgaaatg ggcgcataga gatccgacac agatttcgtc gattccactt cgccgaaggt ctcgccggcg gtgaccgcgg tgccgataac gggtagctga acgaaaacga cgtcgccaag cgccgactgt gcatagtcgg tgatccccac ccggacggtg tcgtcgccac tgcggcgaat ccactcgtgt tcggcggtgt agtgcagatc ggacgggata tcgctcacca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga tggtgatgag ccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSDIPSDL HYTAEHEWIR RSGDDTVRVG ITDYAQSALG DVVFVQLPVI GTAVTAGETF GEVESTKSVS DLYAPISGKV SEVNSDLDGT PQLVNSDPYG AGWLLDIQVD SSDVAALESA LTTLLDAEAY RGTLTE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgagcgat atcccgtccg atctgcacta caccgccgaa cacgagtgga ttcgccgcag tggcgacgac accgtccggg tggggatcac cgactatgca cagtcggcgc ttggcgacgt cgttttcgtt cagctacccg ttatcggcac cgcggtcacc gccggcgaga ccttcggcga agtggaatcg acgaaatctg tgtcggatct ctatgcgccc atttcgggta aggtgtctga ggtcaacagc gatctggacg gcactccgca attggtgaat tccgacccct acggagccgg ctggctgctg gacatccagg tcgacagctc ggatgtcgct gccctggagt cagctttgac gacactgctc gacgctgagg cctaccgcgg cacactgacc gagaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa