MytuD.00980.a

Phosphoserine aminotransferase (EC 2.6.1.52) (Phosphohydroxythreonine aminotransferase) (PSAT) (Rv0884c )

CENTER ID: MytuD.00980.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:
IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
MytuD.00980.a.B1.GE43829 full length 1 376
MytuD.00980.a.HX1.GE43830 full length 1 376
MytuD.00980.a.MB1.GE43831 full length 1 376
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
MytuD.00980.a.B1.PW38949 full length 1 376

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.986
TubercuList: Rv0884c
MTB Network Portal: Rv0884c
UniProt: P9WQ73

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MADQLTPHLE IPTAIKPRDG RFGSGPSKVR LEQLQTLTTT AAALFGTSHR QAPVKNLVGR VRSGLAELFS LPDGYEVILG NGGATAFWDA AAFGLIDKRS LHLTYGEFSA KFASAVSKNP FVGEPIIITS DPGSAPEPQT DPSVDVIAWA HNETSTGVAV AVRRPEGSDD ALVVIDATSG AGGLPVDIAE TDAYYFAPQK NFASDGGLWL AIMSPAALSR IEAIAATGRW VPDFLSLPIA VENSLKNQTY NTPAIATLAL LAEQIDWLVG NGGLDWAVKR TADSSQRLYS WAQERPYTTP FVTDPGLRSQ VVGTIDFVDD VDAGTVAKIL RANGIVDTEP YRKLGRNQLR VAMFPAVEPD DVSALTECVD WVVERL
NT Sequence
atggccgacc agctcacccc ccacctggaa attcccaccg ccatcaaacc ccgcgacggc cgctttgggt cgggcccgtc gaaggtccgg cttgagcaac tgcaaacgct gaccaccacc gcagcggcat tgttcggcac gtcgcaccga caggcgccgg tcaagaatct ggtgggcagg gttcggtcag gcctggccga gctgttctcg ttgccggacg gctatgaggt gatactgggc aatggtggtg ccacggcgtt ctgggatgcg gccgccttcg ggctgattga caaacgctca ctgcacttga cttacggcga gttcagcgcg aagttcgctt ccgccgtttc caagaacccg ttcgtcggcg agccgatcat catcacgtcg gatcccggca gtgctccgga gccgcaaacc gacccgtcag tcgatgtgat cgcctgggcc cacaacgaga cctcgaccgg ggtcgcggtt gcggtgcgcc gtccggaggg ctccgatgac gccttggtcg tcatcgacgc cacctccggt gctggcggcc taccggtcga catcgccgag accgacgcct actacttcgc accgcagaag aacttcgcca gcgacggcgg cctgtggctg gccatcatga gcccggccgc cctgagccgg atcgaggcca tcgccgcgac cggtcgctgg gttcctgatt tcctgtccct gccgatcgcg gtcgagaaca gcttgaagaa ccagacatac aacacaccgg ccatcgccac gctggcgctg ctggccgaac agatcgactg gctggtgggt aacggtggct tggactgggc ggtcaaacgc accgcggact cgtcgcagcg gttgtactcg tgggcgcaag agcggcccta caccacgcca tttgtcaccg accccgggtt gcggtcgcag gtggtgggca cgatcgactt cgtcgacgac gtcgacgccg ggaccgtcgc gaagatcctg cgggctaacg gcatcgtcga caccgagccg tatcgcaaac tgggccgaaa ccagctgcgg gtcgcgatgt tccccgcggt cgagccggac gacgtcagcg ccctcaccga gtgcgtcgac tgggtggtcg agcggcttta g
Details for MytuD.00980.a.B1.GE43829
HARVESTED ON: 8/24/2020
SEQUENCED ON: 8/28/2020
EXPECTED MW: 41kDa
OBSERVED MW: 41kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMA DQLTPHLEIP TAIKPRDGRF GSGPSKVRLE QLQTLTTTAA ALFGTSHRQA PVKNLVGRVR SGLAELFSLP DGYEVILGNG GATAFWDAAA FGLIDKRSLH LTYGEFSAKF ASAVSKNPFV GEPIIITSDP GSAPEPQTDP SVDVIAWAHN ETSTGVAVAV RRPEGSDDAL VVIDATSGAG GLPVDIAETD AYYFAPQKNF ASDGGLWLAI MSPAALSRIE AIAATGRWVP DFLSLPIAVE NSLKNQTYNT PAIATLALLA EQIDWLVGNG GLDWAVKRTA DSSQRLYSWA QERPYTTPFV TDPGLRSQVV GTIDFVDDVD AGTVAKILRA NGIVDTEPYR KLGRNQLRVA MFPAVEPDDV SALTECVDWV VERL
Validated NT Sequence
atggctcacc accaccacca ccatatggcc gaccagctca ccccccacct ggaaattccc accgccatca aaccccgcga cggccgcttt gggtcgggcc cgtcgaaggt ccggcttgag caactgcaaa cgctgaccac caccgcagcg gcattgttcg gcacgtcgca ccgacaggcg ccggtcaaga atctggtggg cagggttcgg tcaggcctgg ccgagctgtt ctcgttgccg gacggctatg aggtgatact gggcaatggt ggtgccacgg cgttctggga tgcggccgcc ttcgggctga ttgacaaacg ctcactgcac ttgacttacg gcgagttcag cgcgaagttc gcttccgccg tttccaagaa cccgttcgtc ggcgagccga tcatcatcac gtcggatccc ggcagtgctc cggagccgca aaccgacccg tcagtcgatg tgatcgcctg ggcccacaac gagacctcga ccggggtcgc ggttgcggtg cgccgtccgg agggctccga tgacgccttg gtcgtcatcg acgccacctc cggtgctggc ggcctaccgg tcgacatcgc cgagaccgac gcctactact tcgcaccgca gaagaacttc gccagcgacg gcggcctgtg gctggccatc atgagcccgg ccgccctgag ccggatcgag gccatcgccg cgaccggtcg ctgggttcct gatttcctgt ccctgccgat cgcggtcgag aacagcttga agaaccagac atacaacaca ccggnccatc gccacgctgg cgctgctggc cgaacagatc gactggctgg tgggtaacgg tggcttggac tgggncggtc aaacgcaccg cggactcgtc gcagcggttg tactcgtggg cgcaagagcg gccctacacc acgccatttg tcaccgaccc cgggttgcgg tcgcaggtgg tgggcacgat cgacttcgtc gacgacgtcg acgccgggac cgtcgcgaag atcctgcggg ctaacggcat cgtcgacacc gagccgtatc gcaaactggg ccgaaaccag ctgcgggtcg cgatgttccc cgcggtcgag ccggacgacg tcagcgccct caccgagtgc gtcgac
Expected Protein Sequence
MAHHHHHHMA DQLTPHLEIP TAIKPRDGRF GSGPSKVRLE QLQTLTTTAA ALFGTSHRQA PVKNLVGRVR SGLAELFSLP DGYEVILGNG GATAFWDAAA FGLIDKRSLH LTYGEFSAKF ASAVSKNPFV GEPIIITSDP GSAPEPQTDP SVDVIAWAHN ETSTGVAVAV RRPEGSDDAL VVIDATSGAG GLPVDIAETD AYYFAPQKNF ASDGGLWLAI MSPAALSRIE AIAATGRWVP DFLSLPIAVE NSLKNQTYNT PAIATLALLA EQIDWLVGNG GLDWAVKRTA DSSQRLYSWA QERPYTTPFV TDPGLRSQVV GTIDFVDDVD AGTVAKILRA NGIVDTEPYR KLGRNQLRVA MFPAVEPDDV SALTECVDWV VERL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggccg accagctcac cccccacctg gaaattccca ccgccatcaa accccgcgac ggccgctttg ggtcgggccc gtcgaaggtc cggcttgagc aactgcaaac gctgaccacc accgcagcgg cattgttcgg cacgtcgcac cgacaggcgc cggtcaagaa tctggtgggc agggttcggt caggcctggc cgagctgttc tcgttgccgg acggctatga ggtgatactg ggcaatggtg gtgccacggc gttctgggat gcggccgcct tcgggctgat tgacaaacgc tcactgcact tgacttacgg cgagttcagc gcgaagttcg cttccgccgt ttccaagaac ccgttcgtcg gcgagccgat catcatcacg tcggatcccg gcagtgctcc ggagccgcaa accgacccgt cagtcgatgt gatcgcctgg gcccacaacg agacctcgac cggggtcgcg gttgcggtgc gccgtccgga gggctccgat gacgccttgg tcgtcatcga cgccacctcc ggtgctggcg gcctaccggt cgacatcgcc gagaccgacg cctactactt cgcaccgcag aagaacttcg ccagcgacgg cggcctgtgg ctggccatca tgagcccggc cgccctgagc cggatcgagg ccatcgccgc gaccggtcgc tgggttcctg atttcctgtc cctgccgatc gcggtcgaga acagcttgaa gaaccagaca tacaacacac cggccatcgc cacgctggcg ctgctggccg aacagatcga ctggctggtg ggtaacggtg gcttggactg ggcggtcaaa cgcaccgcgg actcgtcgca gcggttgtac tcgtgggcgc aagagcggcc ctacaccacg ccatttgtca ccgaccccgg gttgcggtcg caggtggtgg gcacgatcga cttcgtcgac gacgtcgacg ccgggaccgt cgcgaagatc ctgcgggcta acggcatcgt cgacaccgag ccgtatcgca aactgggccg aaaccagctg cgggtcgcga tgttccccgc ggtcgagccg gacgacgtca gcgccctcac cgagtgcgtc gactgggtgg tcgagcggct ttgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
Details for MytuD.00980.a.HX1.GE43830
HARVESTED ON: 8/24/2020
SEQUENCED ON: 8/28/2020
EXPECTED MW: 97kDa
OBSERVED MW: 97kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMK NKEILAVVEA VSNEKALPRE KIFEALESAL ATATKKKYEQ EIDVRVQIDR KSGDFDTFRR WLVVDEVTQP TKEITLEAAR YEDESLNLGD YVEDQIESVT FDRITTQTAK QVIVQKVREA ERAMVVDQFR EHEGEIITGV VKKVNRDNIS LDLGNNAEAV ILREDMLPRE NFRPGDRVRG VLYSVRPEAR GAQLFVTRSK PEMLIELFRI EVPEIGEEVI EIKAAARDPG SRAKIAVKTN DKRIDPVGAC VGMRGARVQA VSTELGGERI DIVLWDDNPA QFVINAMAPA DVASIVVDED KHTMDIAVEA GNLAQAIGRN GQNVRLASQL SGWELNVMTV DDLQAKHQAE AHAAIDTFTK YLDIDEDFAT VLVEEGFSTL EELAYVPMKE LLEIEGLDEP TVEALRERAK NALATIAQAQ EESLGDNKPA DDLLNLEGVD RDLAFKLAAR GVCTLEDLAE QGIDDLADIE GLTDEKAGAL IMAARNICWF GDEAGTLEAQ TQGPGSMADQ LTPHLEIPTA IKPRDGRFGS GPSKVRLEQL QTLTTTAAAL FGTSHRQAPV KNLVGRVRSG LAELFSLPDG YEVILGNGGA TAFWDAAAFG LIDKRSLHLT YGEFSAKFAS AVSKNPFVGE PIIITSDPGS APEPQTDPSV DVIAWAHNET STGVAVAVRR PEGSDDALVV IDATSGAGGL PVDIAETDAY YFAPQKNFAS DGGLWLAIMS PAALSRIEAI AATGRWVPDF LSLPIAVENS LKNQTYNTPA IATLALLAEQ IDWLVGNGGL DWAVKRTADS SQRLYSWAQE RPYTTPFVTD PGLRSQVVGT IDFVDDVDAG TVAKILRANG IVDTEPYRKL GRNQLRVAMF PAVEPDDVSA LTECVDWVVE RL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgaagaacaa agaaattttg gctgtagttg aagccgtatc caatgaaaag gcgctacctc gcgagaagat tttcgaagca ttggaaagcg cgctggcgac agcaacaaag aaaaaatatg aacaagagat cgacgtccgc gtacagatcg atcgcaaaag cggtgatttt gacactttcc gtcgctggtt agttgttgat gaagtcaccc agccgaccaa ggaaatcacc cttgaagccg cacgttatga agatgaaagc ctgaacctgg gcgattacgt tgaagatcag attgagtctg ttacctttga ccgtatcact acccagacgg caaaacaggt tatcgtgcag aaagtgcgtg aagccgaacg tgcgatggtg gttgatcagt tccgtgaaca cgaaggtgaa atcatcaccg gcgtggtgaa aaaagtaaac cgcgacaaca tctctctgga tctgggcaac aacgctgaag ccgtgatcct gcgcgaagat atgctgccgc gtgaaaactt ccgccctggc gaccgcgttc gtggcgtgct ctattccgtt cgcccggaag cgcgtggcgc gcaactgttc gtcactcgtt ccaagccgga aatgctgatc gaactgttcc gtattgaagt gccagaaatc ggcgaagaag tgattgaaat taaagcagcg gctcgcgatc cgggttctcg tgcgaaaatc gcggtgaaaa ccaacgataa acgtatcgat ccggtaggtg cttgcgtagg tatgcgtggc gcgcgtgttc aggcggtgtc tactgaactg ggtggcgagc gtatcgatat cgtcctgtgg gatgataacc cggcgcagtt cgtgattaac gcaatggcac cggcagacgt tgcttctatc gtggtggatg aagataaaca caccatggat atcgccgttg aagccggtaa cctggcgcag gcgattggcc gtaacggtca gaacgtgcgt ctggcttcgc agctgagcgg ttgggaactc aacgtgatga ccgttgacga cctgcaggct aagcatcagg cggaagcgca cgcagcgatc gacaccttca ccaaatatct cgacatcgac gaagacttcg cgactgttct ggtagaagaa ggcttctcga cgctggaaga attggcctat gtgccgatga aagagctgtt ggaaatcgaa ggccttgatg agccgaccgt tgaagcactg cgcgagcgtg ctaaaaatgc actggccacc attgcacagg cccaggaaga aagcctcggt gataacaaac cggctgacga tctgctgaac cttgaagggg tagatcgtga tttggcattc aaactggccg cccgtggcgt ttgtacgctg gaagatctcg ccgaacaggg cattgatgat ctggctgata tcgaagggtt gaccgacgaa aaagccggag cactgattat ggctgcccgt aacatttgct ggttcggtga cgaagcgggt accctggaag ctcagaccca gggtcctggt tcgatggccg accagctcac cccccacctg gaaattccca ccgccatcaa accccgcgac ggccgctttg ggtcgggccc gtcgaaggtc cggcttgagc aactgcaaac gctgaccacc accgcagcgg cattgttcgg cacgtcgcac cgacaggcgc cggtcaagaa tctggtgggc agggttcggt caggcctggc cgagctgttc tcgttgccgg acggctatga ggtgatactg ggcaatggtg gtgccacggc gttctgggat gcggccgcct tcgggctgat tgacaaacgc tcactgcact tgacttacgg cgagttcagc gcgaagttcg cttccgccgt ttccaagaac ccgttcgtcg gcgagccgat catcatcacg tcggatcccg gcagtgctcc ggagccgcaa accgacccgt cagtcgatgt gatcgcctgg gcccacaacg agacctcgac cggggtcgcg gttgcggtgc gccgtccgga gggctccgat gacgccttgg tcgtcatcga cgccacctcc ggtgctggcg gcctaccggt cgacatcgcc gagaccgacg cctactactt cgcaccgcag aagaacttcg ccagcgacgg cggcctgtgg ctggccatca tgagcccggc cgccctgagc cggatcgagg ccatcgccgc gaccggtcgc tgggttcctg atttcctgtc cctgccgatc gcggtcgaga acagcttgaa gaaccagaca tacaacacac cggccatcgc cacgctggcg ctgctggccg aacagatcga ctggctggtg ggtaacggtg gcttggactg ggcggtcaaa cgcaccgcgg actcgtcgca gcggttgtac tcgtgggcgc aagagcggcc ctacaccacg ccatttgtca ccgaccccgg gttgcggtcg caggtggtgg gcacgatcga cttcgtcgac gacgtcgacg ccgggaccgt cgcgaagatc ctgcgggcta acggcatcgt cgacaccgag ccgtatcgca aactgggccg aaaccagctg cgggtcgcga tgttccccgc ggtcgagccg gacgacgtca gcgccctcac cgagtgcgtc gactgggtgg tcgagcggct taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for MytuD.00980.a.MB1.GE43831
HARVESTED ON: 8/24/2020
SEQUENCED ON: 8/28/2020
EXPECTED MW: 85kDa
OBSERVED MW: 85kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 95
PERCENT COVERAGE: 37
Validated AA Sequence
MXXXGXXGCG XXGLIDKRSL HLTYGEFSAK FASAVSXXPF VGEPIIITSD PXSAPEPQTD PSVDVIAWAH NETSTGVAVA VRRPEGSDDA LVVIDATSGA GGLPVDIAET DAYYFAPQKN FASDGGLWLA IMSPAALSRI EAIAATGRWV PDFLSLPIAV ENSLKNQTYN TPAIATLALL AEQIDWLVGN GGLDWAVKRT ADSSQRLYSW AQERPYTTPF VTDPGLRSQV VGTIDFVDDV DAGTVAKILR ANGIVDTEPY RKLGRNQLRV AMFPAVEPDD VSALTECVDW VVERL
Validated NT Sequence
tcnnnnnnnn gttagcagcc ggatcctcga gaagcttggc tgcagaactt gttcgtgctg tttattaaag ccgctcgacc acccagtcga cgcactcggt gagggcgctg acgtcgtccg gctcgaccgc ggggaacatc gcgacccgca gctggtttcg gcccagtttg cgatacggct cggtgtcgac gatgccgtta gcccgcagga tcttcgcgac ggtcccggcg tcgacgtcgt cgacgaagtc gatcgtgccc accacctgcg accgcaaccc ggggtcggtg acaaatggcg tggtgtaggg ccgctcttgc gcccacgagt acaaccgctg cgacgagtcc gcggtgcgtt tgaccgccca gtccaagcca ccgttaccca ccagccagtc gatctgttcg gccagcagcg ccagcgtggc gatggccggt gtgttgtatg tctggttctt caagctgttc tcgaccgcga tcggcaggga caggaaatca ggaacccagc gaccggtcgc ggcgatggcc tcgatccggc tcagggcggc cgggctcatg atggccagcc acaggccgcc gtcgctggcg aagttcttct gcggtgcgaa gtagtaggcg tcggtctcgg cgatgtcgac cggtaggccg ccagcaccgg aggtggcgtc gatgacgacc aaggcgtcat cggagccctc cggacggcgc accgcaaccg cgaccccggt cgaggtctcg ttgtgggccc aggcgatcac atcgactgac gggtcggttt gcggctccgg agcactgncg ggatccgacg tgatgatgat cggctcgccg acgaacgggt ncntggaaac ggcggaagcg aacttcgcgc tgaactcgcc gtaagtcaag tgcagtgagc gtttgtcaat cagcccgann nggccgcatc ccanaangcc gnnnnccncc attgcccagt atcaccctca tagccgtccg gcaacganna anagctnggn naggnntgac cgaaccctnc ccnccanatt cntgacngnn nctgtcngng cgannnnccn annannncnn nnnngnngnn nnagcnttgc nnnnncnnna nncnnnnnnn cnnnngnnnc nannnanngn ngnnnncggg nnnngannnn nnnggnantn nnc
Expected Protein Sequence
MAHHHHHHMK IEEGKLVIWI NGDKGYNGLA EVGKKFEKDT GIKVTVEHPD KLEEKFPQVA ATGDGPDIIF WAHDRFGGYA QSGLLAEITP DKAFQDKLYP FTWDAVRYNG KLIAYPIAVE ALSLIYNKDL LPNPPKTWEE IPALDKELKA KGKSALMFNL QEPYFTWPLI AADGGYAFKY ENGKYDIKDV GVDNAGAKAG LTFLVDLIKN KHMNADTDYS IAEAAFNKGE TAMTINGPWA WSNIDTSKVN YGVTVLPTFK GQPSKPFVGV LSAGINAASP NKELAKEFLE NYLLTDEGLE AVNKDKPLGA VALKSYEEEL AKDPRIAATM ENAQKGEIMP NIPQMSAFWY AVRTAVINAA SGRQTVDEAL KDAQTNSSSN NNNNNNNNNL GIEGRISEGT LEAQTQGPGS MADQLTPHLE IPTAIKPRDG RFGSGPSKVR LEQLQTLTTT AAALFGTSHR QAPVKNLVGR VRSGLAELFS LPDGYEVILG NGGATAFWDA AAFGLIDKRS LHLTYGEFSA KFASAVSKNP FVGEPIIITS DPGSAPEPQT DPSVDVIAWA HNETSTGVAV AVRRPEGSDD ALVVIDATSG AGGLPVDIAE TDAYYFAPQK NFASDGGLWL AIMSPAALSR IEAIAATGRW VPDFLSLPIA VENSLKNQTY NTPAIATLAL LAEQIDWLVG NGGLDWAVKR TADSSQRLYS WAQERPYTTP FVTDPGLRSQ VVGTIDFVDD VDAGTVAKIL RANGIVDTEP YRKLGRNQLR VAMFPAVEPD DVSALTECVD WVVERL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcaca tgaaaatcga agaaggtaaa ctggtaatct ggattaacgg cgataaaggc tataacggtc tcgctgaagt cggtaagaaa ttcgagaaag ataccggaat taaagtcacc gttgagcatc cggataaact ggaagagaaa ttcccacagg ttgcggcaac tggcgatggc cctgacatta tcttctgggc acacgaccgc tttggtggct acgctcaatc tggcctgttg gctgaaatca ccccggacaa agcgttccag gacaagctgt atccgtttac ctgggatgcc gtacgttaca acggcaagct gattgcttac ccgatcgctg ttgaagcgtt atcgctgatt tataacaaag atctgctgcc gaacccgcca aaaacctggg aagagatccc ggcgctggat aaagaactga aagcgaaagg taagagcgcg ctgatgttca acctgcaaga accgtacttc acctggccgc tgattgctgc tgacgggggt tatgcgttca agtatgaaaa cggcaagtac gacattaaag acgtgggcgt ggataacgct ggcgcgaaag cgggtctgac cttcctggtt gacctgatta aaaacaaaca catgaatgca gacaccgatt actccatcgc agaagctgcc tttaataaag gcgaaacagc gatgaccatc aacggcccgt gggcatggtc caacatcgac accagcaaag tgaattatgg tgtaacggta ctgccgacct tcaagggtca accatccaaa ccgttcgttg gcgtgctgag cgcaggtatt aacgccgcca gtccgaacaa agagctggca aaagagttcc tcgaaaacta tctgctgact gatgaaggtc tggaagcggt taataaagac aaaccgctgg gtgccgtagc gctgaagtct tacgaggaag agttggcgaa agatccacgt attgccgcca ctatggaaaa cgcccagaaa ggtgaaatca tgccgaacat cccgcagatg tccgctttct ggtatgccgt gcgtactgcg gtgatcaacg ccgccagcgg tcgtcagact gtcgatgaag ccctgaaaga cgcgcagact aattcgagct cgaacaacaa caacaacaac aataacaaca acctcgggat cgagggaagg atttcagaag gtaccctgga agctcagacc cagggtcctg gttcgatggc cgaccagctc accccccacc tggaaattcc caccgccatc aaaccccgcg acggccgctt tgggtcgggc ccgtcgaagg tccggcttga gcaactgcaa acgctgacca ccaccgcagc ggcattgttc ggcacgtcgc accgacaggc gccggtcaag aatctggtgg gcagggttcg gtcaggcctg gccgagctgt tctcgttgcc ggacggctat gaggtgatac tgggcaatgg tggtgccacg gcgttctggg atgcggccgc cttcgggctg attgacaaac gctcactgca cttgacttac ggcgagttca gcgcgaagtt cgcttccgcc gtttccaaga acccgttcgt cggcgagccg atcatcatca cgtcggatcc cggcagtgct ccggagccgc aaaccgaccc gtcagtcgat gtgatcgcct gggcccacaa cgagacctcg accggggtcg cggttgcggt gcgccgtccg gagggctccg atgacgcctt ggtcgtcatc gacgccacct ccggtgctgg cggcctaccg gtcgacatcg ccgagaccga cgcctactac ttcgcaccgc agaagaactt cgccagcgac ggcggcctgt ggctggccat catgagcccg gccgccctga gccggatcga ggccatcgcc gcgaccggtc gctgggttcc tgatttcctg tccctgccga tcgcggtcga gaacagcttg aagaaccaga catacaacac accggccatc gccacgctgg cgctgctggc cgaacagatc gactggctgg tgggtaacgg tggcttggac tgggcggtca aacgcaccgc ggactcgtcg cagcggttgt actcgtgggc gcaagagcgg ccctacacca cgccatttgt caccgacccc gggttgcggt cgcaggtggt gggcacgatc gacttcgtcg acgacgtcga cgccgggacc gtcgcgaaga tcctgcgggc taacggcatc gtcgacaccg agccgtatcg caaactgggc cgaaaccagc tgcgggtcgc gatgttcccc gcggtcgagc cggacgacgt cagcgccctc accgagtgcg tcgactgggt ggtcgagcgg ctttaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for MytuD.00980.a.B1.PW38949
PURIFICATION DATe: 3/1/2021
CONCENTRATION: 20mg/ml
OBSERVED MW: 41kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 18
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Protocol Notes
protein precipitation prior to freezing, recommend spinning down prior to setting up trays
Validated AA Sequence
MAHHHHHHMA DQLTPHLEIP TAIKPRDGRF GSGPSKVRLE QLQTLTTTAA ALFGTSHRQA PVKNLVGRVR SGLAELFSLP DGYEVILGNG GATAFWDAAA FGLIDKRSLH LTYGEFSAKF ASAVSKNPFV GEPIIITSDP GSAPEPQTDP SVDVIAWAHN ETSTGVAVAV RRPEGSDDAL VVIDATSGAG GLPVDIAETD AYYFAPQKNF ASDGGLWLAI MSPAALSRIE AIAATGRWVP DFLSLPIAVE NSLKNQTYNT PAIATLALLA EQIDWLVGNG GLDWAVKRTA DSSQRLYSWA QERPYTTPFV TDPGLRSQVV GTIDFVDDVD AGTVAKILRA NGIVDTEPYR KLGRNQLRVA MFPAVEPDDV SALTECVDWV VERL
Validated NT Sequence
atggctcacc accaccacca ccatatggcc gaccagctca ccccccacct ggaaattccc accgccatca aaccccgcga cggccgcttt gggtcgggcc cgtcgaaggt ccggcttgag caactgcaaa cgctgaccac caccgcagcg gcattgttcg gcacgtcgca ccgacaggcg ccggtcaaga atctggtggg cagggttcgg tcaggcctgg ccgagctgtt ctcgttgccg gacggctatg aggtgatact gggcaatggt ggtgccacgg cgttctggga tgcggccgcc ttcgggctga ttgacaaacg ctcactgcac ttgacttacg gcgagttcag cgcgaagttc gcttccgccg tttccaagaa cccgttcgtc ggcgagccga tcatcatcac gtcggatccc ggcagtgctc cggagccgca aaccgacccg tcagtcgatg tgatcgcctg ggcccacaac gagacctcga ccggggtcgc ggttgcggtg cgccgtccgg agggctccga tgacgccttg gtcgtcatcg acgccacctc cggtgctggc ggcctaccgg tcgacatcgc cgagaccgac gcctactact tcgcaccgca gaagaacttc gccagcgacg gcggcctgtg gctggccatc atgagcccgg ccgccctgag ccggatcgag gccatcgccg cgaccggtcg ctgggttcct gatttcctgt ccctgccgat cgcggtcgag aacagcttga agaaccagac atacaacaca ccggnccatc gccacgctgg cgctgctggc cgaacagatc gactggctgg tgggtaacgg tggcttggac tgggncggtc aaacgcaccg cggactcgtc gcagcggttg tactcgtggg cgcaagagcg gccctacacc acgccatttg tcaccgaccc cgggttgcgg tcgcaggtgg tgggcacgat cgacttcgtc gacgacgtcg acgccgggac cgtcgcgaag atcctgcggg ctaacggcat cgtcgacacc gagccgtatc gcaaactggg ccgaaaccag ctgcgggtcg cgatgttccc cgcggtcgag ccggacgacg tcagcgccct caccgagtgc gtcgac
Expressed Protein Sequence
MAHHHHHHMA DQLTPHLEIP TAIKPRDGRF GSGPSKVRLE QLQTLTTTAA ALFGTSHRQA PVKNLVGRVR SGLAELFSLP DGYEVILGNG GATAFWDAAA FGLIDKRSLH LTYGEFSAKF ASAVSKNPFV GEPIIITSDP GSAPEPQTDP SVDVIAWAHN ETSTGVAVAV RRPEGSDDAL VVIDATSGAG GLPVDIAETD AYYFAPQKNF ASDGGLWLAI MSPAALSRIE AIAATGRWVP DFLSLPIAVE NSLKNQTYNT PAIATLALLA EQIDWLVGNG GLDWAVKRTA DSSQRLYSWA QERPYTTPFV TDPGLRSQVV GTIDFVDDVD AGTVAKILRA NGIVDTEPYR KLGRNQLRVA MFPAVEPDDV SALTECVDWV VERL
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCCG ACCAGCTCAC CCCCCACCTG GAAATTCCCA CCGCCATCAA ACCCCGCGAC GGCCGCTTTG GGTCGGGCCC GTCGAAGGTC CGGCTTGAGC AACTGCAAAC GCTGACCACC ACCGCAGCGG CATTGTTCGG CACGTCGCAC CGACAGGCGC CGGTCAAGAA TCTGGTGGGC AGGGTTCGGT CAGGCCTGGC CGAGCTGTTC TCGTTGCCGG ACGGCTATGA GGTGATACTG GGCAATGGTG GTGCCACGGC GTTCTGGGAT GCGGCCGCCT TCGGGCTGAT TGACAAACGC TCACTGCACT TGACTTACGG CGAGTTCAGC GCGAAGTTCG CTTCCGCCGT TTCCAAGAAC CCGTTCGTCG GCGAGCCGAT CATCATCACG TCGGATCCCG GCAGTGCTCC GGAGCCGCAA ACCGACCCGT CAGTCGATGT GATCGCCTGG GCCCACAACG AGACCTCGAC CGGGGTCGCG GTTGCGGTGC GCCGTCCGGA GGGCTCCGAT GACGCCTTGG TCGTCATCGA CGCCACCTCC GGTGCTGGCG GCCTACCGGT CGACATCGCC GAGACCGACG CCTACTACTT CGCACCGCAG AAGAACTTCG CCAGCGACGG CGGCCTGTGG CTGGCCATCA TGAGCCCGGC CGCCCTGAGC CGGATCGAGG CCATCGCCGC GACCGGTCGC TGGGTTCCTG ATTTCCTGTC CCTGCCGATC GCGGTCGAGA ACAGCTTGAA GAACCAGACA TACAACACAC CGGCCATCGC CACGCTGGCG CTGCTGGCCG AACAGATCGA CTGGCTGGTG GGTAACGGTG GCTTGGACTG GGCGGTCAAA CGCACCGCGG ACTCGTCGCA GCGGTTGTAC TCGTGGGCGC AAGAGCGGCC CTACACCACG CCATTTGTCA CCGACCCCGG GTTGCGGTCG CAGGTGGTGG GCACGATCGA CTTCGTCGAC GACGTCGACG CCGGGACCGT CGCGAAGATC CTGCGGGCTA ACGGCATCGT CGACACCGAG CCGTATCGCA AACTGGGCCG AAACCAGCTG CGGGTCGCGA TGTTCCCCGC GGTCGAGCCG GACGACGTCA GCGCCCTCAC CGAGTGCGTC GACTGGGTGG TCGAGCGGCT TTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT