MytuD.00891.a

Probable oxidoreductase

CENTER ID: MytuD.00891.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: diffraction
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:
IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
MytuD.00891.a.A1.GE25916 Full length( MytuD.00891.a ) 1 270
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
MytuD.00891.a.A1.PW26028 Full length( MytuD.00891.a ) 1 270

External Resources

RESOURCE REFERENCE ID
OrthoMCL: OG5_131031
PATRIC ID: fig|83332.12.peg.1907
RefSeq: NP_216230.1
TubercuList: Rv1714
MTB Network Portal: Rv1714
UniProt: P9WGQ3

Orthologues for Rv1714

Species % Identity % Coverage Clones Proteins Structures
abscessus 49 94 1 1 0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEEMALAQQV PNLGLARFSV QDKSILITGA TGSLGRVAAR ALADAGARLT LAGGNSAGLA ELVNGAGIDD AAVVTCRPDS LADAQQMVEA ALGRYGRLDG VLVASGSNHV APITEMAVED FDAVMDANVR GAWLVCRAAG RVLLEQGQGG SVVLVSSVRG GLGNAAGYSA YCPSKAGTDL LAKTLAAEWG GHGIRVNALA PTVFRSAVTE WMFTDDPKGR ATREAMLARI PLRRFAEPED FVGALIYLLS DASSFYTGQV MYLDGGYTAC
NT Sequence
GTGGAGGAAA TGGCGCTGGC TCAGCAGGTG CCGAACCTGG GTCTGGCGCG CTTCAGCGTG CAGGACAAGT CGATCCTGAT CACCGGCGCG ACCGGTTCGT TGGGCCGAGT TGCCGCCCGG GCGCTGGCCG ACGCGGGAGC GCGGCTGACA CTGGCCGGCG GCAACTCGGC CGGTCTGGCC GAGCTGGTCA ACGGCGCCGG CATCGACGAC GCCGCCGTCG TGACCTGCCG GCCGGACAGC CTGGCCGATG CCCAGCAGAT GGTCGAGGCG GCACTGGGCC GATATGGCCG TTTGGACGGA GTGTTGGTGG CCTCGGGCAG CAACCATGTG GCGCCCATTA CCGAGATGGC CGTCGAGGAC TTCGACGCTG TGATGGACGC GAACGTGCGG GGTGCCTGGC TGGTGTGTCG GGCGGCCGGA CGGGTGCTGC TCGAGCAGGG TCAGGGCGGC AGCGTGGTGC TGGTGTCGTC CGTTCGCGGC GGGTTGGGCA ATGCCGCCGG TTACAGCGCG TACTGCCCGT CGAAGGCGGG CACCGATCTG TTGGCCAAGA CATTGGCGGC CGAATGGGGC GGTCACGGCA TTCGGGTGAA CGCGCTGGCG CCGACGGTGT TTCGGTCCGC GGTGACCGAG TGGATGTTCA CCGACGATCC GAAGGGCCGG GCCACCCGGG AGGCGATGCT CGCCCGGATC CCGTTGCGCC GCTTCGCCGA ACCGGAAGAC TTCGTCGGCG CCCTGATCTA TCTGCTCAGC GACGCCTCGA GCTTCTACAC CGGCCAGGTG ATGTATCTGG ACGGCGGGTA CACCGCATGC
Details for MytuD.00891.a.A1.GE25916
HARVESTED ON: 1/9/2009
SEQUENCED ON: 8/18/2010
EXPECTED MW: 30kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAQTQG PGSMVEEMAL AQQVPNLGLA RFSVQDKSIL ITGATGSLGR VAARALADAG ARLTLAGGNS AGLAELVNGA GIDDAAVVTC RPDSLADAQQ MVEAALGRYG RLDGVLVASG SNHVAPITEM AVEDFDAVMD ANVRGAWLVC RAAGRVLLEQ GQGGSVVLVS SVRGGLGNAA GYSAYCPSKA GTDLLAKTLA AEWGGHGIRV NALAPTVFRS AVTEWMFTDD PKGRATREAM LARIPLRRFA EPEDFVGALI YLLSDASSFY TGQVMYLDGG YTAC
Validated NT Sequence
tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatggtgga ggaaatggcg ctggctcagc aggtgccgaa cctgggtctg gcgcgcttca gcgtgcagga caagtcgatc ctgatcaccg gcgcgaccgg ttcgttgggc cgagttgccg cccgggcgct ggccgacgcg ggagcgcggc tgacactggc cggcggcaac tcggccggtc tggccgagct ggtcaacggc gccggcatcg acgacgccgc cgtcgtgacc tgccggccgg acagcctggc cgatgcccag cagatggtcg aggcggcact gggccgatat ggccgtttgg acggagtgtt ggtggcctcg ggcagcaacc atgtggcgcc cattaccgag atggccgtcg aggacttcga cgctgtgatg gacgcgaacg tgcggggtgc ctggctggtg tgtcgggcgg ccggacgggt gctgctcgag cagggtcagg gcggcagcgt ggtgctggtg tcgtccgttc gcggcgggtt gggcaatgcc gccggttaca gcgcgtactg cccgtcgaag gcgggcaccg atctgttggc caagacattg gcggccgaat ggggcggtca cggcattcgg gtgaacgcgc tggcgccgac ggtgtttcgg tccgcggtga ccgagtggat gttcaccgac gatccgaagg gccgggccac ccgggaggcg atgctcgccc ggatcccgtt gcgccgcttc gccgaaccgg aagacttcgt cggcgccctg atctatctgc tcagcgacgc ctcgagcttc tacaccggcc aggtgatgta tctggacggc gggtacaccg catgctaata a
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVEEMALAQ QVPNLGLARF SVQDKSILIT GATGSLGRVA ARALADAGAR LTLAGGNSAG LAELVNGAGI DDAAVVTCRP DSLADAQQMV EAALGRYGRL DGVLVASGSN HVAPITEMAV EDFDAVMDAN VRGAWLVCRA AGRVLLEQGQ GGSVVLVSSV RGGLGNAAGY SAYCPSKAGT DLLAKTLAAE WGGHGIRVNA LAPTVFRSAV TEWMFTDDPK GRATREAMLA RIPLRRFAEP EDFVGALIYL LSDASSFYTG QVMYLDGGYT AC
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtggaggaa atggcgctgg ctcagcaggt gccgaacctg ggtctggcgc gcttcagcgt gcaggacaag tcgatcctga tcaccggcgc gaccggttcg ttgggccgag ttgccgcccg ggcgctggcc gacgcgggag cgcggctgac actggccggc ggcaactcgg ccggtctggc cgagctggtc aacggcgccg gcatcgacga cgccgccgtc gtgacctgcc ggccggacag cctggccgat gcccagcaga tggtcgaggc ggcactgggc cgatatggcc gtttggacgg agtgttggtg gcctcgggca gcaaccatgt ggcgcccatt accgagatgg ccgtcgagga cttcgacgct gtgatggacg cgaacgtgcg gggtgcctgg ctggtgtgtc gggcggccgg acgggtgctg ctcgagcagg gtcagggcgg cagcgtggtg ctggtgtcgt ccgttcgcgg cgggttgggc aatgccgccg gttacagcgc gtactgcccg tcgaaggcgg gcaccgatct gttggccaag acattggcgg ccgaatgggg cggtcacggc attcgggtga acgcgctggc gccgacggtg tttcggtccg cggtgaccga gtggatgttc accgacgatc cgaagggccg ggccacccgg gaggcgatgc tcgcccggat cccgttgcgc cgcttcgccg aaccggaaga cttcgtcggc gccctgatct atctgctcag cgacgcctcg agcttctaca ccggccaggt gatgtatctg gacggcgggt acaccgcatg caaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for MytuD.00891.a.A1.PW26028
PURIFICATION DATe: 3/31/2009
CONCENTRATION: 25.38mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 13
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMVEEMAL AQQVPNLGLA RFSVQDKSIL ITGATGSLGR VAARALADAG ARLTLAGGNS AGLAELVNGA GIDDAAVVTC RPDSLADAQQ MVEAALGRYG RLDGVLVASG SNHVAPITEM AVEDFDAVMD ANVRGAWLVC RAAGRVLLEQ GQGGSVVLVS SVRGGLGNAA GYSAYCPSKA GTDLLAKTLA AEWGGHGIRV NALAPTVFRS AVTEWMFTDD PKGRATREAM LARIPLRRFA EPEDFVGALI YLLSDASSFY TGQVMYLDGG YTAC
Validated NT Sequence
tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatggtgga ggaaatggcg ctggctcagc aggtgccgaa cctgggtctg gcgcgcttca gcgtgcagga caagtcgatc ctgatcaccg gcgcgaccgg ttcgttgggc cgagttgccg cccgggcgct ggccgacgcg ggagcgcggc tgacactggc cggcggcaac tcggccggtc tggccgagct ggtcaacggc gccggcatcg acgacgccgc cgtcgtgacc tgccggccgg acagcctggc cgatgcccag cagatggtcg aggcggcact gggccgatat ggccgtttgg acggagtgtt ggtggcctcg ggcagcaacc atgtggcgcc cattaccgag atggccgtcg aggacttcga cgctgtgatg gacgcgaacg tgcggggtgc ctggctggtg tgtcgggcgg ccggacgggt gctgctcgag cagggtcagg gcggcagcgt ggtgctggtg tcgtccgttc gcggcgggtt gggcaatgcc gccggttaca gcgcgtactg cccgtcgaag gcgggcaccg atctgttggc caagacattg gcggccgaat ggggcggtca cggcattcgg gtgaacgcgc tggcgccgac ggtgtttcgg tccgcggtga ccgagtggat gttcaccgac gatccgaagg gccgggccac ccgggaggcg atgctcgccc ggatcccgtt gcgccgcttc gccgaaccgg aagacttcgt cggcgccctg atctatctgc tcagcgacgc ctcgagcttc tacaccggcc aggtgatgta tctggacggc gggtacaccg catgctaata a
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVEEMALAQ QVPNLGLARF SVQDKSILIT GATGSLGRVA ARALADAGAR LTLAGGNSAG LAELVNGAGI DDAAVVTCRP DSLADAQQMV EAALGRYGRL DGVLVASGSN HVAPITEMAV EDFDAVMDAN VRGAWLVCRA AGRVLLEQGQ GGSVVLVSSV RGGLGNAAGY SAYCPSKAGT DLLAKTLAAE WGGHGIRVNA LAPTVFRSAV TEWMFTDDPK GRATREAMLA RIPLRRFAEP EDFVGALIYL LSDASSFYTG QVMYLDGGYT AC
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGGAGGAA ATGGCGCTGG CTCAGCAGGT GCCGAACCTG GGTCTGGCGC GCTTCAGCGT GCAGGACAAG TCGATCCTGA TCACCGGCGC GACCGGTTCG TTGGGCCGAG TTGCCGCCCG GGCGCTGGCC GACGCGGGAG CGCGGCTGAC ACTGGCCGGC GGCAACTCGG CCGGTCTGGC CGAGCTGGTC AACGGCGCCG GCATCGACGA CGCCGCCGTC GTGACCTGCC GGCCGGACAG CCTGGCCGAT GCCCAGCAGA TGGTCGAGGC GGCACTGGGC CGATATGGCC GTTTGGACGG AGTGTTGGTG GCCTCGGGCA GCAACCATGT GGCGCCCATT ACCGAGATGG CCGTCGAGGA CTTCGACGCT GTGATGGACG CGAACGTGCG GGGTGCCTGG CTGGTGTGTC GGGCGGCCGG ACGGGTGCTG CTCGAGCAGG GTCAGGGCGG CAGCGTGGTG CTGGTGTCGT CCGTTCGCGG CGGGTTGGGC AATGCCGCCG GTTACAGCGC GTACTGCCCG TCGAAGGCGG GCACCGATCT GTTGGCCAAG ACATTGGCGG CCGAATGGGG CGGTCACGGC ATTCGGGTGA ACGCGCTGGC GCCGACGGTG TTTCGGTCCG CGGTGACCGA GTGGATGTTC ACCGACGATC CGAAGGGCCG GGCCACCCGG GAGGCGATGC TCGCCCGGAT CCCGTTGCGC CGCTTCGCCG AACCGGAAGA CTTCGTCGGC GCCCTGATCT ATCTGCTCAG CGACGCCTCG AGCTTCTACA CCGGCCAGGT GATGTATCTG GACGGCGGGT ACACCGCATG CAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA