MytuD.00783.a
serine hydroxymethyltransferase
| CENTER ID: | MytuD.00783.a |
| ORGANISM: | Mycobacterium tuberculosis H37Rv |
| ASSOCIATED DISEASE: | Tuberculosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MytuD.00783.a.A1.GE25909 | Full length( MytuD.00783.a ) | 1 | 426 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MytuD.00783.a.A1.PS00175a | Full length( MytuD.00783.a ) | 1 | 426 | ||
| MytuD.00783.a.A1.PW26018 | Full length( MytuD.00783.a ) | 1 | 426 |
Structures
3H7F
DEPOSITED: 4/24/2009
DETERMINATION: XRay
CLONE:
PROTEIN: MytuD.00783.a.A1.PS00175
6ULD
DEPOSITED: 10/7/2019
DETERMINATION: XRay
CLONE:
PROTEIN: MytuD.00783.a.A1.PS00175
Publications by SSGCID
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_126679 |
| PATRIC ID: | fig|83332.12.peg.1224 |
| RefSeq: | YP_177787.1 |
| TubercuList: | Rv1093 |
| MTB Network Portal: | Rv1093 |
| UniProt: | P9WGI9 |
Orthologues for Rv1093
| Species | % Identity | % Coverage | Clones | Proteins | Structures |
|---|---|---|---|---|---|
| leprae | 90 | 100 | 0 | 0 | 0 |
| avium | 89 | 100 | 0 | 0 | 0 |
| paratuberculosis | 88 | 100 | 0 | 0 | 0 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSAPLAEVDP DIAELLAKEL GRQRDTLEMI ASENFVPRAV LQAQGSVLTN KYAEGLPGRR YYGGCEHVDV VENLARDRAK ALFGAEFANV QPHSGAQANA AVLHALMSPG ERLLGLDLAN GGHLTHGMRL NFSGKLYENG FYGVDPATHL IDMDAVRATA LEFRPKVIIA GWSAYPRVLD FAAFRSIADE VGAKLLVDMA HFAGLVAAGL HPSPVPHADV VSTTVHKTLG GGRSGLIVGK QQYAKAINSA VFPGQQGGPL MHVIAGKAVA LKIAATPEFA DRQRRTLSGA RIIADRLMAP DVAKAGVSVV SGGTDVHLVL VDLRDSPLDG QAAEDLLHEV GITVNRNAVP NDPRPPMVTS GLRIGTPALA TRGFGDTEFT EVADIIATAL ATGSSVDVSA LKDRATRLAR AFPLYDGLEE WSLVGR
NT Sequence
ATGTCTGCCC CGCTCGCTGA GGTTGACCCC GATATCGCCG AGTTGCTGGC CAAGGAGCTT GGTCGGCAAC GAGACACCCT GGAGATGATC GCCTCGGAGA ACTTCGTACC GCGCGCTGTG CTGCAGGCCC AGGGCAGTGT GCTGACCAAC AAGTACGCCG AGGGACTGCC CGGGCGGCGC TACTACGGCG GTTGTGAGCA CGTCGACGTG GTGGAAAACC TCGCCCGCGA CCGAGCCAAG GCGTTGTTCG GTGCCGAATT CGCCAATGTG CAACCGCATT CGGGCGCTCA GGCCAACGCC GCGGTGCTGC ATGCGCTGAT GTCACCCGGC GAGCGGCTGT TGGGTCTGGA CCTGGCCAAC GGTGGTCACC TGACCCATGG CATGCGGCTG AACTTCTCCG GCAAGCTCTA CGAGAATGGC TTCTACGGCG TCGACCCGGC GACACATCTG ATCGACATGG ATGCGGTGCG GGCCACCGCA CTCGAATTCC GCCCGAAGGT GATCATCGCC GGCTGGTCGG CCTACCCGCG GGTGCTCGAC TTCGCGGCGT TCCGGTCGAT CGCCGACGAG GTCGGGGCCA AGTTGCTCGT GGACATGGCG CATTTCGCGG GTCTGGTCGC CGCGGGGTTG CACCCGTCGC CGGTGCCGCA CGCGGATGTG GTGTCCACCA CCGTGCACAA GACGCTCGGC GGCGGCCGCT CCGGCCTGAT CGTCGGTAAG CAGCAGTACG CCAAGGCGAT CAACTCGGCG GTGTTTCCCG GGCAGCAGGG CGGTCCGCTC ATGCACGTCA TTGCCGGCAA GGCGGTCGCG TTGAAGATCG CCGCCACACC CGAATTTGCC GACCGGCAGC GGCGCACGCT GTCCGGGGCC CGGATCATTG CCGATCGACT GATGGCTCCC GATGTCGCCA AGGCCGGTGT GTCGGTGGTC AGCGGCGGCA CCGACGTCCA CCTGGTGCTG GTCGATCTGC GTGATTCCCC ACTGGATGGC CAGGCCGCCG AGGACCTGCT GCACGAGGTC GGCATCACGG TCAACCGCAA CGCCGTCCCC AATGATCCCC GACCGCCGAT GGTGACCTCG GGCCTGCGGA TAGGCACGCC CGCGCTGGCG ACCCGCGGCT TCGGCGACAC CGAGTTCACC GAGGTCGCCG ACATTATTGC GACCGCGCTG GCGACCGGCA GTTCCGTTGA TGTGTCGGCG CTTAAGGATC GGGCGACCCG GCTGGCCAGG GCGTTTCCGC TCTACGACGG GCTCGAGGAG TGGAGTCTGG TCGGCCGC
Details for MytuD.00783.a.A1.GE25909
| HARVESTED ON: | 1/9/2009 |
| SEQUENCED ON: | 8/18/2010 |
| EXPECTED MW: | 47kDa |
| OBSERVED MW: | 48kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 98 |
Validated AA Sequence
MGTLEAQTQG PGSMSAPLAX VDPDIAELLA KELGRQRDTL EMIASENFVP RAVLQAQGSV LTNKYAEGLP GRRYYGGCEH VDVVENLARD RAKALFGAEF ANVQPHSGAQ ANAAVLHALM SPGERLLGLD LANGGHLTHG MRLNFSGKLY ENGFYGVDPA THLIDMDAVR ATALEFRPKV IIAGWSAYPR VLDFAAFRSI ADEVGAKLLV DMAHFAGLVA AGLHPSPVPH ADVVSTTVHK TLGGGRSGLI VGKQQYAKAI NSAVFPGQQG GPLMHVIAGK AVALKIAATP EFADRQRRTL SGARIIADRL MAPDVAKAGV SVVSGGTDVH LVLVDLRDSP LDGQAAEDLL HEVGITVNRN AVPNDPRPPM VTSGLRIGTP ALATRGFGDT EFTEVADIIA TALATGSSVD VSALKDRATR LARAFPLYDG LEEWSLVGR
Validated NT Sequence
tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgtctgc cccgctcgct gangttgacc ccgatatcgc cgagttgctg gccaaggagc ttggtcggca acgagacacc ctggagatga tcgcctcgga gaacttcgta ccgcgcgctg tgctgcaggc ccagggcagt gtgctgacca acaagtacgc cgagggactg cccgggcggc gctactacgg cggttgtgag cacgtcgacg tggtggaaaa cctcgcccgc gaccgagcca aggcgttgtt cggtgccgaa ttcgccaatg tgcaaccgca ttcgggcgct caggccaacg ccgcggtgct gcatgcgctg atgtcacccg gcgagcggct gttgggtctg gacctggcca acggtggtca cctgacccat ggcatgcggc tgaacttctc cggcaagctc tacgagaatg gcttctacgg cgtcgacccg gcgacacatc tgatcgacat ggatgcggtg cgggccaccg cactcgaatt ccgcccgaag gtgatcatcg ccggctggtc ggcctacccg cgggtgctcg acttcgcggc gttccggtcg atcgccgacg aggtcggggc caagttgctc gtggacatgg cgcatttcgc gggtctggtc gccgcggggt tgcacccgtc gccggtgccg cacgcggatg tggtgtccac caccgtgcac aagacgctcg gcggcggccg ctccggcctg atcgtcggta agcagcagta cgccaaggcg atcaactcgg cggtgtttcc cgggcagcag ggcggtccgc tcatgcacgt cattgccggc aaggcggtcg cgttgaagat cgccgccaca cccgaatttg ccgaccggca gcggcgcacg ctgtccgggg cccggatcat tgccgatcga ctgatggctc ccgatgtcgc caaggccggt gtgtcggtgg tcagcggcgg caccgacgtc cacctggtgc tggtcgatct gcgtgattcc ccactggatg gccaggccgc cgaggacctg ctgcacgagg tcggcatcac ggtcaaccgc aacgccgtcc ccaatgatcc ccgaccgccg atggtgacct cgggcctgcg gataggcacg cccgcgctgg cgacccgcgg cttcggcgac accgagttca ccgaggtcgc cgacattatt gcgaccgcgc tggcgaccgg cagttccgtt gatgtgtcgg cgcttaagga tcgggcgacc cggctggcca gggcgtttcc gctctacgac gggctcgagg agtggagtct ggtcggccgn taataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSAPLAEVD PDIAELLAKE LGRQRDTLEM IASENFVPRA VLQAQGSVLT NKYAEGLPGR RYYGGCEHVD VVENLARDRA KALFGAEFAN VQPHSGAQAN AAVLHALMSP GERLLGLDLA NGGHLTHGMR LNFSGKLYEN GFYGVDPATH LIDMDAVRAT ALEFRPKVII AGWSAYPRVL DFAAFRSIAD EVGAKLLVDM AHFAGLVAAG LHPSPVPHAD VVSTTVHKTL GGGRSGLIVG KQQYAKAINS AVFPGQQGGP LMHVIAGKAV ALKIAATPEF ADRQRRTLSG ARIIADRLMA PDVAKAGVSV VSGGTDVHLV LVDLRDSPLD GQAAEDLLHE VGITVNRNAV PNDPRPPMVT SGLRIGTPAL ATRGFGDTEF TEVADIIATA LATGSSVDVS ALKDRATRLA RAFPLYDGLE EWSLVGR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtctgccccg ctcgctgagg ttgaccccga tatcgccgag ttgctggcca aggagcttgg tcggcaacga gacaccctgg agatgatcgc ctcggagaac ttcgtaccgc gcgctgtgct gcaggcccag ggcagtgtgc tgaccaacaa gtacgccgag ggactgcccg ggcggcgcta ctacggcggt tgtgagcacg tcgacgtggt ggaaaacctc gcccgcgacc gagccaaggc gttgttcggt gccgaattcg ccaatgtgca accgcattcg ggcgctcagg ccaacgccgc ggtgctgcat gcgctgatgt cacccggcga gcggctgttg ggtctggacc tggccaacgg tggtcacctg acccatggca tgcggctgaa cttctccggc aagctctacg agaatggctt ctacggcgtc gacccggcga cacatctgat cgacatggat gcggtgcggg ccaccgcact cgaattccgc ccgaaggtga tcatcgccgg ctggtcggcc tacccgcggg tgctcgactt cgcggcgttc cggtcgatcg ccgacgaggt cggggccaag ttgctcgtgg acatggcgca tttcgcgggt ctggtcgccg cggggttgca cccgtcgccg gtgccgcacg cggatgtggt gtccaccacc gtgcacaaga cgctcggcgg cggccgctcc ggcctgatcg tcggtaagca gcagtacgcc aaggcgatca actcggcggt gtttcccggg cagcagggcg gtccgctcat gcacgtcatt gccggcaagg cggtcgcgtt gaagatcgcc gccacacccg aatttgccga ccggcagcgg cgcacgctgt ccggggcccg gatcattgcc gatcgactga tggctcccga tgtcgccaag gccggtgtgt cggtggtcag cggcggcacc gacgtccacc tggtgctggt cgatctgcgt gattccccac tggatggcca ggccgccgag gacctgctgc acgaggtcgg catcacggtc aaccgcaacg ccgtccccaa tgatccccga ccgccgatgg tgacctcggg cctgcggata ggcacgcccg cgctggcgac ccgcggcttc ggcgacaccg agttcaccga ggtcgccgac attattgcga ccgcgctggc gaccggcagt tccgttgatg tgtcggcgct taaggatcgg gcgacccggc tggccagggc gtttccgctc tacgacgggc tcgaggagtg gagtctggtc ggccgcaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for MytuD.00783.a.A1.PS00175a
| PURIFICATION DATe: | 2/18/2009 |
| CONCENTRATION: | 20.2mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 2 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 98 |
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMSAPLAX VDPDIAELLA KELGRQRDTL EMIASENFVP RAVLQAQGSV LTNKYAEGLP GRRYYGGCEH VDVVENLARD RAKALFGAEF ANVQPHSGAQ ANAAVLHALM SPGERLLGLD LANGGHLTHG MRLNFSGKLY ENGFYGVDPA THLIDMDAVR ATALEFRPKV IIAGWSAYPR VLDFAAFRSI ADEVGAKLLV DMAHFAGLVA AGLHPSPVPH ADVVSTTVHK TLGGGRSGLI VGKQQYAKAI NSAVFPGQQG GPLMHVIAGK AVALKIAATP EFADRQRRTL SGARIIADRL MAPDVAKAGV SVVSGGTDVH LVLVDLRDSP LDGQAAEDLL HEVGITVNRN AVPNDPRPPM VTSGLRIGTP ALATRGFGDT EFTEVADIIA TALATGSSVD VSALKDRATR LARAFPLYDG LEEWSLVGR
Validated NT Sequence
tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgtctgc cccgctcgct gangttgacc ccgatatcgc cgagttgctg gccaaggagc ttggtcggca acgagacacc ctggagatga tcgcctcgga gaacttcgta ccgcgcgctg tgctgcaggc ccagggcagt gtgctgacca acaagtacgc cgagggactg cccgggcggc gctactacgg cggttgtgag cacgtcgacg tggtggaaaa cctcgcccgc gaccgagcca aggcgttgtt cggtgccgaa ttcgccaatg tgcaaccgca ttcgggcgct caggccaacg ccgcggtgct gcatgcgctg atgtcacccg gcgagcggct gttgggtctg gacctggcca acggtggtca cctgacccat ggcatgcggc tgaacttctc cggcaagctc tacgagaatg gcttctacgg cgtcgacccg gcgacacatc tgatcgacat ggatgcggtg cgggccaccg cactcgaatt ccgcccgaag gtgatcatcg ccggctggtc ggcctacccg cgggtgctcg acttcgcggc gttccggtcg atcgccgacg aggtcggggc caagttgctc gtggacatgg cgcatttcgc gggtctggtc gccgcggggt tgcacccgtc gccggtgccg cacgcggatg tggtgtccac caccgtgcac aagacgctcg gcggcggccg ctccggcctg atcgtcggta agcagcagta cgccaaggcg atcaactcgg cggtgtttcc cgggcagcag ggcggtccgc tcatgcacgt cattgccggc aaggcggtcg cgttgaagat cgccgccaca cccgaatttg ccgaccggca gcggcgcacg ctgtccgggg cccggatcat tgccgatcga ctgatggctc ccgatgtcgc caaggccggt gtgtcggtgg tcagcggcgg caccgacgtc cacctggtgc tggtcgatct gcgtgattcc ccactggatg gccaggccgc cgaggacctg ctgcacgagg tcggcatcac ggtcaaccgc aacgccgtcc ccaatgatcc ccgaccgccg atggtgacct cgggcctgcg gataggcacg cccgcgctgg cgacccgcgg cttcggcgac accgagttca ccgaggtcgc cgacattatt gcgaccgcgc tggcgaccgg cagttccgtt gatgtgtcgg cgcttaagga tcgggcgacc cggctggcca gggcgtttcc gctctacgac gggctcgagg agtggagtct ggtcggccgn taataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSAPLAEVD PDIAELLAKE LGRQRDTLEM IASENFVPRA VLQAQGSVLT NKYAEGLPGR RYYGGCEHVD VVENLARDRA KALFGAEFAN VQPHSGAQAN AAVLHALMSP GERLLGLDLA NGGHLTHGMR LNFSGKLYEN GFYGVDPATH LIDMDAVRAT ALEFRPKVII AGWSAYPRVL DFAAFRSIAD EVGAKLLVDM AHFAGLVAAG LHPSPVPHAD VVSTTVHKTL GGGRSGLIVG KQQYAKAINS AVFPGQQGGP LMHVIAGKAV ALKIAATPEF ADRQRRTLSG ARIIADRLMA PDVAKAGVSV VSGGTDVHLV LVDLRDSPLD GQAAEDLLHE VGITVNRNAV PNDPRPPMVT SGLRIGTPAL ATRGFGDTEF TEVADIIATA LATGSSVDVS ALKDRATRLA RAFPLYDGLE EWSLVGR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCTGCCCCG CTCGCTGAGG TTGACCCCGA TATCGCCGAG TTGCTGGCCA AGGAGCTTGG TCGGCAACGA GACACCCTGG AGATGATCGC CTCGGAGAAC TTCGTACCGC GCGCTGTGCT GCAGGCCCAG GGCAGTGTGC TGACCAACAA GTACGCCGAG GGACTGCCCG GGCGGCGCTA CTACGGCGGT TGTGAGCACG TCGACGTGGT GGAAAACCTC GCCCGCGACC GAGCCAAGGC GTTGTTCGGT GCCGAATTCG CCAATGTGCA ACCGCATTCG GGCGCTCAGG CCAACGCCGC GGTGCTGCAT GCGCTGATGT CACCCGGCGA GCGGCTGTTG GGTCTGGACC TGGCCAACGG TGGTCACCTG ACCCATGGCA TGCGGCTGAA CTTCTCCGGC AAGCTCTACG AGAATGGCTT CTACGGCGTC GACCCGGCGA CACATCTGAT CGACATGGAT GCGGTGCGGG CCACCGCACT CGAATTCCGC CCGAAGGTGA TCATCGCCGG CTGGTCGGCC TACCCGCGGG TGCTCGACTT CGCGGCGTTC CGGTCGATCG CCGACGAGGT CGGGGCCAAG TTGCTCGTGG ACATGGCGCA TTTCGCGGGT CTGGTCGCCG CGGGGTTGCA CCCGTCGCCG GTGCCGCACG CGGATGTGGT GTCCACCACC GTGCACAAGA CGCTCGGCGG CGGCCGCTCC GGCCTGATCG TCGGTAAGCA GCAGTACGCC AAGGCGATCA ACTCGGCGGT GTTTCCCGGG CAGCAGGGCG GTCCGCTCAT GCACGTCATT GCCGGCAAGG CGGTCGCGTT GAAGATCGCC GCCACACCCG AATTTGCCGA CCGGCAGCGG CGCACGCTGT CCGGGGCCCG GATCATTGCC GATCGACTGA TGGCTCCCGA TGTCGCCAAG GCCGGTGTGT CGGTGGTCAG CGGCGGCACC GACGTCCACC TGGTGCTGGT CGATCTGCGT GATTCCCCAC TGGATGGCCA GGCCGCCGAG GACCTGCTGC ACGAGGTCGG CATCACGGTC AACCGCAACG CCGTCCCCAA TGATCCCCGA CCGCCGATGG TGACCTCGGG CCTGCGGATA GGCACGCCCG CGCTGGCGAC CCGCGGCTTC GGCGACACCG AGTTCACCGA GGTCGCCGAC ATTATTGCGA CCGCGCTGGC GACCGGCAGT TCCGTTGATG TGTCGGCGCT TAAGGATCGG GCGACCCGGC TGGCCAGGGC GTTTCCGCTC TACGACGGGC TCGAGGAGTG GAGTCTGGTC GGCCGCAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA
Details for MytuD.00783.a.A1.PW26018
| PURIFICATION DATe: | 2/17/2009 |
| CONCENTRATION: | 23.56mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 1 |
| VIAL VOLUME: | 30µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 98 |
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMSAPLAX VDPDIAELLA KELGRQRDTL EMIASENFVP RAVLQAQGSV LTNKYAEGLP GRRYYGGCEH VDVVENLARD RAKALFGAEF ANVQPHSGAQ ANAAVLHALM SPGERLLGLD LANGGHLTHG MRLNFSGKLY ENGFYGVDPA THLIDMDAVR ATALEFRPKV IIAGWSAYPR VLDFAAFRSI ADEVGAKLLV DMAHFAGLVA AGLHPSPVPH ADVVSTTVHK TLGGGRSGLI VGKQQYAKAI NSAVFPGQQG GPLMHVIAGK AVALKIAATP EFADRQRRTL SGARIIADRL MAPDVAKAGV SVVSGGTDVH LVLVDLRDSP LDGQAAEDLL HEVGITVNRN AVPNDPRPPM VTSGLRIGTP ALATRGFGDT EFTEVADIIA TALATGSSVD VSALKDRATR LARAFPLYDG LEEWSLVGR
Validated NT Sequence
tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgtctgc cccgctcgct gangttgacc ccgatatcgc cgagttgctg gccaaggagc ttggtcggca acgagacacc ctggagatga tcgcctcgga gaacttcgta ccgcgcgctg tgctgcaggc ccagggcagt gtgctgacca acaagtacgc cgagggactg cccgggcggc gctactacgg cggttgtgag cacgtcgacg tggtggaaaa cctcgcccgc gaccgagcca aggcgttgtt cggtgccgaa ttcgccaatg tgcaaccgca ttcgggcgct caggccaacg ccgcggtgct gcatgcgctg atgtcacccg gcgagcggct gttgggtctg gacctggcca acggtggtca cctgacccat ggcatgcggc tgaacttctc cggcaagctc tacgagaatg gcttctacgg cgtcgacccg gcgacacatc tgatcgacat ggatgcggtg cgggccaccg cactcgaatt ccgcccgaag gtgatcatcg ccggctggtc ggcctacccg cgggtgctcg acttcgcggc gttccggtcg atcgccgacg aggtcggggc caagttgctc gtggacatgg cgcatttcgc gggtctggtc gccgcggggt tgcacccgtc gccggtgccg cacgcggatg tggtgtccac caccgtgcac aagacgctcg gcggcggccg ctccggcctg atcgtcggta agcagcagta cgccaaggcg atcaactcgg cggtgtttcc cgggcagcag ggcggtccgc tcatgcacgt cattgccggc aaggcggtcg cgttgaagat cgccgccaca cccgaatttg ccgaccggca gcggcgcacg ctgtccgggg cccggatcat tgccgatcga ctgatggctc ccgatgtcgc caaggccggt gtgtcggtgg tcagcggcgg caccgacgtc cacctggtgc tggtcgatct gcgtgattcc ccactggatg gccaggccgc cgaggacctg ctgcacgagg tcggcatcac ggtcaaccgc aacgccgtcc ccaatgatcc ccgaccgccg atggtgacct cgggcctgcg gataggcacg cccgcgctgg cgacccgcgg cttcggcgac accgagttca ccgaggtcgc cgacattatt gcgaccgcgc tggcgaccgg cagttccgtt gatgtgtcgg cgcttaagga tcgggcgacc cggctggcca gggcgtttcc gctctacgac gggctcgagg agtggagtct ggtcggccgn taataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSAPLAEVD PDIAELLAKE LGRQRDTLEM IASENFVPRA VLQAQGSVLT NKYAEGLPGR RYYGGCEHVD VVENLARDRA KALFGAEFAN VQPHSGAQAN AAVLHALMSP GERLLGLDLA NGGHLTHGMR LNFSGKLYEN GFYGVDPATH LIDMDAVRAT ALEFRPKVII AGWSAYPRVL DFAAFRSIAD EVGAKLLVDM AHFAGLVAAG LHPSPVPHAD VVSTTVHKTL GGGRSGLIVG KQQYAKAINS AVFPGQQGGP LMHVIAGKAV ALKIAATPEF ADRQRRTLSG ARIIADRLMA PDVAKAGVSV VSGGTDVHLV LVDLRDSPLD GQAAEDLLHE VGITVNRNAV PNDPRPPMVT SGLRIGTPAL ATRGFGDTEF TEVADIIATA LATGSSVDVS ALKDRATRLA RAFPLYDGLE EWSLVGR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCTGCCCCG CTCGCTGAGG TTGACCCCGA TATCGCCGAG TTGCTGGCCA AGGAGCTTGG TCGGCAACGA GACACCCTGG AGATGATCGC CTCGGAGAAC TTCGTACCGC GCGCTGTGCT GCAGGCCCAG GGCAGTGTGC TGACCAACAA GTACGCCGAG GGACTGCCCG GGCGGCGCTA CTACGGCGGT TGTGAGCACG TCGACGTGGT GGAAAACCTC GCCCGCGACC GAGCCAAGGC GTTGTTCGGT GCCGAATTCG CCAATGTGCA ACCGCATTCG GGCGCTCAGG CCAACGCCGC GGTGCTGCAT GCGCTGATGT CACCCGGCGA GCGGCTGTTG GGTCTGGACC TGGCCAACGG TGGTCACCTG ACCCATGGCA TGCGGCTGAA CTTCTCCGGC AAGCTCTACG AGAATGGCTT CTACGGCGTC GACCCGGCGA CACATCTGAT CGACATGGAT GCGGTGCGGG CCACCGCACT CGAATTCCGC CCGAAGGTGA TCATCGCCGG CTGGTCGGCC TACCCGCGGG TGCTCGACTT CGCGGCGTTC CGGTCGATCG CCGACGAGGT CGGGGCCAAG TTGCTCGTGG ACATGGCGCA TTTCGCGGGT CTGGTCGCCG CGGGGTTGCA CCCGTCGCCG GTGCCGCACG CGGATGTGGT GTCCACCACC GTGCACAAGA CGCTCGGCGG CGGCCGCTCC GGCCTGATCG TCGGTAAGCA GCAGTACGCC AAGGCGATCA ACTCGGCGGT GTTTCCCGGG CAGCAGGGCG GTCCGCTCAT GCACGTCATT GCCGGCAAGG CGGTCGCGTT GAAGATCGCC GCCACACCCG AATTTGCCGA CCGGCAGCGG CGCACGCTGT CCGGGGCCCG GATCATTGCC GATCGACTGA TGGCTCCCGA TGTCGCCAAG GCCGGTGTGT CGGTGGTCAG CGGCGGCACC GACGTCCACC TGGTGCTGGT CGATCTGCGT GATTCCCCAC TGGATGGCCA GGCCGCCGAG GACCTGCTGC ACGAGGTCGG CATCACGGTC AACCGCAACG CCGTCCCCAA TGATCCCCGA CCGCCGATGG TGACCTCGGG CCTGCGGATA GGCACGCCCG CGCTGGCGAC CCGCGGCTTC GGCGACACCG AGTTCACCGA GGTCGCCGAC ATTATTGCGA CCGCGCTGGC GACCGGCAGT TCCGTTGATG TGTCGGCGCT TAAGGATCGG GCGACCCGGC TGGCCAGGGC GTTTCCGCTC TACGACGGGC TCGAGGAGTG GAGTCTGGTC GGCCGCAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA