MytuD.00698.a
methylcitrate synthase
| CENTER ID: | MytuD.00698.a |
| ORGANISM: | Mycobacterium tuberculosis H37Rv |
| ASSOCIATED DISEASE: | Tuberculosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MytuD.00698.a.A1.GE25873 | Full length( MytuD.00698.a ) | 1 | 393 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
3HWK
DEPOSITED: 6/17/2009
DETERMINATION: XRay
CLONE: MytuD.00698.a.A1.GE25873
PROTEIN: MytuD.00698.a.A1.PW25932
Publications by SSGCID
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|83332.12.peg.1267 |
| RefSeq: | NP_215647.1 |
| TubercuList: | Rv1131 |
| MTB Network Portal: | Rv1131 |
| UniProt: | I6Y9Q3 |
Orthologues for Rv1131
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTGPLAAARS VAATKSMTAP TVDERPDIKK GLAGVVVDTT AISKVVPQTN SLTYRGYPVQ DLAARCSFEQ VAFLLWRGEL PTDAELALFS QRERASRRVD RSMLSLLAKL PDNCHPMDVV RTAISYLGAE DPDEDDAAAN RAKAMRMMAV LPTIVAIDMR RRRGLPPIAP HSGLGYAQNF LHMCFGEVPE TAVVSAFEQS MILYAEHGFN ASTFAARVVT STQSDIYSAV TGAIGALKGR LHGGANEAVM HDMIEIGDPA NAREWLRAKL ARKEKIMGFG HRVYRHGDSR VPTMKRALER VGTVRDGQRW LDIYQVLAAE MASATGILPN LDFPTGPAYY LMGFDIASFT PIFVMSRITG WTAHIMEQAT ANALIRPLSA YCGHEQRVLP GTF
NT Sequence
ATGACCGGGC CGCTCGCGGC GGCCAGGTCC GTCGCTGCCA CGAAATCGAT GACCGCGCCC ACCGTTGATG AGCGGCCCGA CATCAAAAAG GGCCTCGCCG GCGTGGTGGT GGACACCACC GCCATCTCCA AGGTGGTGCC GCAGACCAAT TCGTTGACCT ACCGGGGATA TCCGGTCCAG GATCTGGCAG CCCGCTGCAG TTTCGAGCAG GTCGCCTTCC TGCTGTGGCG TGGTGAGTTG CCCACCGATG CCGAGCTGGC GTTGTTCAGC CAGCGCGAAC GAGCCAGCCG TCGGGTGGAC CGCTCGATGC TGTCATTGCT GGCCAAGCTG CCGGACAACT GCCACCCGAT GGACGTGGTG CGCACCGCGA TCAGCTATCT CGGTGCCGAG GACCCGGACG AGGACGACGC CGCGGCCAAC CGGGCCAAGG CGATGCGCAT GATGGCGGTG TTGCCGACGA TCGTGGCGAT CGACATGCGG CGCCGACGCG GGTTGCCCCC GATCGCACCG CACAGCGGGC TCGGTTATGC GCAGAACTTC CTGCACATGT GCTTCGGGGA GGTACCCGAA ACCGCCGTCG TGTCGGCGTT CGAGCAGTCG ATGATCCTCT ACGCCGAGCA CGGATTCAAC GCGTCGACGT TCGCCGCCCG GGTGGTGACC TCGACCCAAT CCGACATCTA CAGCGCGGTG ACCGGCGCGA TCGGCGCCCT CAAGGGGCGG CTACACGGCG GCGCCAACGA AGCCGTCATG CACGACATGA TCGAGATCGG CGATCCGGCC AACGCGCGGG AGTGGTTGCG CGCCAAGCTC GCCCGCAAGG AAAAGATCAT GGGCTTCGGG CATCGGGTGT ACCGGCACGG CGACTCCCGG GTGCCGACCA TGAAACGGGC GCTGGAGCGC GTGGGGACCG TTCGCGACGG CCAGCGATGG CTGGACATCT ACCAGGTGTT AGCGGCCGAG ATGGCGTCGG CCACCGGGAT CTTGCCCAAC CTCGATTTTC CGACCGGGCC CGCGTACTAC CTGATGGGAT TCGACATCGC CAGCTTCACC CCGATCTTCG TGATGAGTAG GATCACCGGC TGGACCGCAC ACATCATGGA ACAGGCCACG GCCAACGCGC TGATCCGGCC GCTGAGCGCA TATTGCGGGC ACGAGCAGCG GGTGTTACCG GGCACCTTC
Details for MytuD.00698.a.A1.GE25873
| HARVESTED ON: | 11/19/2008 |
| SEQUENCED ON: | 8/18/2010 |
| EXPECTED MW: | 45kDa |
| OBSERVED MW: | 45kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 98 |
Validated AA Sequence
MGTLEAQTQG PGSMTGPLAA ARSVAATKSM TAPTVDERPD IKKGLAGVVV DTTAISKVVP QTNSLTYRGY PVQDLAARCS FEQVAFLLWR GELPTDAELA LFSQRERASR RVDRSMLSLL AKLPDNCHPM DVVRTAISYL GAEDPDEDDA AANRAKAMRM MAVLPTIVAI DMRRRRGLPP IAPHSGLGYA QNFLHMCFGE VPETAVVSAF EQSMILYAEH GFNASTFAAR VVTSTQSDIY SAVTGAIGAL KGRLHGGANE AVMHDMIEIG DPANAREWLR AKLARKEKIM GFGHRVYRHG DSRVPTMKRA LERVGTVRDG QRWLDIYQVL AAEMASATGI LPNLDFPTGP AYYLMGFDIA SFTPIFVMSR ITGWTAHIME QATANALIRP LSAYCGHEQR VLPGTF
Validated NT Sequence
tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgaccgg gccgctcgcg gcggccaggt ccgtcgctgc cacgaaatcg atgaccgcgc ccaccgttga tgagcggccc gacatcaaaa agggcctcgc cggcgtggtg gtggacacca ccgccatctc caaggtggtg ccgcagacca attcgttgac ctaccgggga tatccggtcc aggatctggc agcccgctgc agtttcgagc aggtcgcctt cctgctgtgg cgtggtgagt tgcccaccga tgccgagctg gcgttgttca gccagcgcga acgagccagc cgtcgggtgg accgctcgat gctgtcattg ctggccaagc tgccggacaa ctgccacccg atggacgtgg tgcgcaccgc gatcagctat ctcggtgccg aggacccgga cgaggacgac gccgcggcca accgggccaa ggcgatgcgc atgatggcgg tgttgccgac gatcgtggcg atcgacatgc ggcgccgacg cgggttgccc ccgatcgcac cgcacagcgg gctcggttat gcgcagaact tcctgcacat gtgcttcggg gaggtacccg aaaccgccgt cgtgtcggcg ttcgagcagt cgatgatcct ctacgccgag cacggattca acgcgtcgac gttcgccgcc cgggtggtga cctcgaccca atccgacatc tacagcgcgg tgaccggcgc gatcggcgcc ctcaaggggc ggctacacgg cggcgccaac gaagccgtca tgcacgacat gatcgagatc ggcgatccgg ccaacgcgcg ggagtggttg cgcgccaagc tcgcccgcaa ggaaaagatc atgggcttcg ggcatcgggt gtaccggcac ggcgactccc gggtgccgac catgaaacgg gcgctggagc gcgtggggac cgttcgcgac ggccagcgat ggctggacat ctaccaggtg ttagcggccg agatggcgtc ggccaccggg atcttgccca acctcgattt tccgaccggg cccgcgtact acctgatggg attcgacatc gccagcttca ccccgatctt cgtgatgagt aggatcaccg gctggaccgc acacatcatg gaacaggcca cggccaacgc gctgatccgg ccgctgagcg catattgcgg gcacgagcag cgggtgttac cgggcacctt ctaataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTGPLAAAR SVAATKSMTA PTVDERPDIK KGLAGVVVDT TAISKVVPQT NSLTYRGYPV QDLAARCSFE QVAFLLWRGE LPTDAELALF SQRERASRRV DRSMLSLLAK LPDNCHPMDV VRTAISYLGA EDPDEDDAAA NRAKAMRMMA VLPTIVAIDM RRRRGLPPIA PHSGLGYAQN FLHMCFGEVP ETAVVSAFEQ SMILYAEHGF NASTFAARVV TSTQSDIYSA VTGAIGALKG RLHGGANEAV MHDMIEIGDP ANAREWLRAK LARKEKIMGF GHRVYRHGDS RVPTMKRALE RVGTVRDGQR WLDIYQVLAA EMASATGILP NLDFPTGPAY YLMGFDIASF TPIFVMSRIT GWTAHIMEQA TANALIRPLS AYCGHEQRVL PGTF
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaccgggccg ctcgcggcgg ccaggtccgt cgctgccacg aaatcgatga ccgcgcccac cgttgatgag cggcccgaca tcaaaaaggg cctcgccggc gtggtggtgg acaccaccgc catctccaag gtggtgccgc agaccaattc gttgacctac cggggatatc cggtccagga tctggcagcc cgctgcagtt tcgagcaggt cgccttcctg ctgtggcgtg gtgagttgcc caccgatgcc gagctggcgt tgttcagcca gcgcgaacga gccagccgtc gggtggaccg ctcgatgctg tcattgctgg ccaagctgcc ggacaactgc cacccgatgg acgtggtgcg caccgcgatc agctatctcg gtgccgagga cccggacgag gacgacgccg cggccaaccg ggccaaggcg atgcgcatga tggcggtgtt gccgacgatc gtggcgatcg acatgcggcg ccgacgcggg ttgcccccga tcgcaccgca cagcgggctc ggttatgcgc agaacttcct gcacatgtgc ttcggggagg tacccgaaac cgccgtcgtg tcggcgttcg agcagtcgat gatcctctac gccgagcacg gattcaacgc gtcgacgttc gccgcccggg tggtgacctc gacccaatcc gacatctaca gcgcggtgac cggcgcgatc ggcgccctca aggggcggct acacggcggc gccaacgaag ccgtcatgca cgacatgatc gagatcggcg atccggccaa cgcgcgggag tggttgcgcg ccaagctcgc ccgcaaggaa aagatcatgg gcttcgggca tcgggtgtac cggcacggcg actcccgggt gccgaccatg aaacgggcgc tggagcgcgt ggggaccgtt cgcgacggcc agcgatggct ggacatctac caggtgttag cggccgagat ggcgtcggcc accgggatct tgcccaacct cgattttccg accgggcccg cgtactacct gatgggattc gacatcgcca gcttcacccc gatcttcgtg atgagtagga tcaccggctg gaccgcacac atcatggaac aggccacggc caacgcgctg atccggccgc tgagcgcata ttgcgggcac gagcagcggg tgttaccggg caccttcaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa