MytuD.00629.d

Bifunctional enzyme MbtA: salicyl-AMP ligase (SAL-AMP ligase) + salicyl-S-ArCP synthetase (Enterobactin synthase subunit E)

CENTER ID: MytuD.00629.d
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.00629.d.B2.GE39931 ordered domain 19 555
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.293.peg.2660
RefSeq: WP_003412282.1
TubercuList: Rv2384
MTB Network Portal: Rv2384
UniProt: P71716

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPPKAADGRR PSPDGGLGGF VPFPADRAAS YRAAGYWSGR TLDTVLSDAA RRWPDRLAVA DAGDRPGHGG LSYAELDQRA DRAAAALHGL GITPGDRVLL QLPNGCQFAV ALFALLRAGA IPVMCLPGHR AAELGHFAAV SAATGLVVAD VASGFDYRPM ARELVADHPT LRHVIVDGDP GPFVSWAQLC AQAGTGSPAP PADPGSPALL LVSGGTTGMP KLIPRTHDDY VFNATASAAL CRLSADDVYL VVLAAGHNFP LACPGLLGAM TVGATAVFAP DPSPEAAFAA IERHGVTVTA LVPALAKLWA QSCEWEPVTP KSLRLLQVGG SKLEPEDARR VRTALTPGLQ QVFGMAEGLL NFTRIGDPPE VVEHTQGRPL CPADELRIVN ADGEPVGPGE EGELLVRGPY TLNGYFAAER DNERCFDPDG FYRSGDLVRR RDDGNLVVTG RVKDVICRAG ETIAASDLEE QLLSHPAIFS AAAVGLPDQY LGEKICAAVV FAGAPITLAE LNGYLDRRGV AAHTRPDQLV AMPALPTTPI GKIDKRAIVR QLGIATGPVT TQRCH
NT Sequence
atgccaccga aggcggcaga tggccgccga cccagtcccg acggcggact gggtggcttt gtaccgttcc ccgcggatcg ggccgcgtcg taccgggcgg ccggctattg gtcggggcga accctggaca ccgtgctctc cgatgccgcg cggcgctggc ctgaccgcct cgcggtggcc gacgccggtg atcgtcccgg ccacggcggc ctcagttacg ccgaactcga ccagcgggcc gaccgggccg ccgcggcgct gcacggcctg ggcatcacgc caggcgaccg ggtactgctc cagctgccaa acggctgcca gttcgcggtt gccctgttcg cgttattgcg ggcgggagcg atcccagtga tgtgcctgcc cggtcaccgc gccgccgaat tgggccactt cgccgccgtc agcgcggcca ccgggctggt ggtcgccgat gtggccagcg ggttcgacta tcggccgatg gcgcgcgaac ttgttgccga tcaccccacc ctgcgccatg tcatcgtcga tggcgatccg ggaccgttcg tgtcgtgggc gcagctgtgc gcccaggccg gcaccggttc gccggcaccg ccggccgatc ccggatcgcc agcgctgctg ctggtctccg gcggcaccac tggcatgccc aaactcattc cacgcaccca cgacgactac gtgttcaacg cgacggccag cgccgcactc tgtcggctta gcgccgacga cgtctatctg gtggtgctgg ccgccggcca caatttcccg ctggcctgcc cgggcctgct cggcgcgatg accgtcgggg ccaccgccgt gttcgccccc gatcccagcc cggaggccgc cttcgccgcc atcgagcgcc acggtgtcac cgtcaccgcg ttggttccgg cactggccaa actgtgggcc caatcctgtg agtgggagcc ggtgacaccg aagtcactgc ggttgttgca ggttggcggg tccaagctag aacccgagga cgctcgccgg gtacgcaccg cgctcacccc gggcctgcag caggtgttcg gcatggcgga ggggctgctg aacttcaccc gcatcggcga cccacccgaa gtggtggagc acacccaggg gcggccacta tgcccggccg acgaactgcg catcgtcaac gccgatggtg agccggtggg gcccggggag gaaggcgaac tcttggtgcg cgggccctac acgctgaacg gctattttgc tgccgaacgc gacaacgagc gctgcttcga tccggacggc ttctaccgca gcggcgacct ggtccgccgc cgcgacgacg gcaatctggt ggtcaccggg cgcgtcaagg atgtcatctg ccgtgcggga gaaaccatcg ccgccagcga cctcgaagaa cagctgctga gccatccggc gatcttctcg gccgcggcgg tgggactacc tgaccagtat ctgggggaaa aaatctgcgc tgcagtcgtt ttcgctggag ctccgattac gcttgcggag ttgaacggct accttgaccg gcgtggtgtg gccgcgcata cgcgacccga ccagctggtc gcgatgccgg cgctgcccac aacgccgatc gggaagatcg acaaacgagc gatcgtccgc cagctcggca tcgcgacggg tcccgtgacg acccagcgct gccattga
Details for MytuD.00629.d.B2.GE39931
HARVESTED ON: 2/26/2016
SEQUENCED ON: 3/1/2016
EXPECTED MW: 57kDa
OBSERVED MW: 57kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 96
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMG FVPFPADRAA SYRAAGYWSG RTLDTVLSDA ARRWPDRLAV ADAGDRPGHG GLSYAELDQR ADRAAAALHG LGITPGDRVL LQLPNGCQFA VALFALLRAG AIPVMCLPGH RAAELGHFAA VSAATGLVVA DVASGFDYRP MARELVADHP TLRHVIVDGD PGPFVSWAQL CAQAGTGSPA PPADPGSPAL LLVSGGTTGM PKLIPRTHDD YVFNATASAA LCRLSADDVY LVVLAAGHNF PXXCPGLLGA MTVGAXXXXX XXXXPEAAFA AIERHGVTVT AXXPALAKLW AXXCEWEPVT PKSLRLLQVG GSKXXPEDAR RVRTALTPGL QQVFGMAEGL LNFTRIGDPP EVVEHTQGRP LCPADELRIV NADGEPVGPG EEGELLVRGP YTLNGYFAAE RDNERCFDPD GFYRSGDLVR RRDDGNLVVT GRVKDVICRA GETIAASDLE EQLLSHPAIF SAAAVGLPDQ YLGEKICAAV VFAGAPITLA ELNGYLDRRG VAAHTRPDQL VAMPALPTTP IGKIDKRA
Validated NT Sequence
anngctcgtt tgtcgatctt cccgatcggc gttgtgggca gcgccggcat cgcgaccagc tggtcgggtc gcgtatgcgc ggccacacca cgccggtcaa ggtagccgtt caactccgca agcgtaatcg gagctccagc gaaaacgact gcagcgcaga ttttttcccc cagatactgg tcaggtagtc ccaccgccgc ggccgagaag atcgccggat ggctcagcag ctgttcttcg aggtcgctgg cggcgatggt ttctcccgca cggcagatga catccttgac gcgcccggtg accaccagat tgccgtcgtc gcggcggcgg accaggtcgc cgctgcggta gaagccgtcc ggatcgaagc agcgctcgtt gtcgcgttcg gcagcaaaat agccgttcag cgtgtagggc ccgcgcacca agagttcgcc ttcctccccg ggccccaccg gctcaccatc ggcgttgacg atgcgcagtt cgtcggccgg gcatagtggc cgcccctggg tgtgctccac cacttcgggt gggtcgccga tgcgggtgaa gttcagcagc ccctccgcca tgccgaacac ctgctgcagg cccggggtga gcgcggtgcg tacccggcga gcgtcctcgg gttnnngctt ggacccgcca acctgcaaca accgcagtga cttcggtgtc accggctccc actcacaggn nngggcccac agtttggcca gtgccggnnn nnacgcggtg acggtgacac cgtggcgctc gatggcggcg aaggcggcct ccgggnnnnn ancgngnnnn nannnnncnn nngccccnac ggtcatcgcg ccgagcaggc ccgggcagnn nngcgggaaa ttgtggccgg cggccagcac caccagatag acgtcgtcgg cgctaagccg acagagtgcg gcgctggccg tcgcgttgaa cacgtagtcg tcgtgggtgc gtggaatgag tttgggcatg ccagtggtgc cgccggagac cagcagcagc gctggcgatc cgggatcggc cggcggtgcc ggcgaaccgg tgccggcctg ggcgcacagc tgcgcccacg acacgaacgg tcccggatcg ccatcgacga tgacatggcg cagggtgggg tgatcggcaa caagttcgcg cgccatcggc cgatagtcga acccgctggc cacatcggcg accaccagcc cggtggccgc gctgacggcg gcgaagtggc ccaattcggc ggcgcggtga ccgggcaggc acatcactgg gatcgctccc gcccgcaata acgcgaacag ggcaaccgcg aactggcagc cgtttggcag ctggagcagt acccggtcgc ctggcgtgat gcccaggccg tgcagcgccg cggcggcccg gtcggcccgc tggtcgagtt cggcgtaact gaggccgccg tggccgggac gatcaccggc gtcggccacc gcgaggcggt caggccagcg ccgcgcggca tcggagagca cggtgtccag ggttcgcccc gaccaatagc cggccgcccg gtacgacgcg gcccgatccg cggggaacgg tacaaagccc atatggtggt ggtggtggtg agccat
Expected Protein Sequence
MAHHHHHHGF VPFPADRAAS YRAAGYWSGR TLDTVLSDAA RRWPDRLAVA DAGDRPGHGG LSYAELDQRA DRAAAALHGL GITPGDRVLL QLPNGCQFAV ALFALLRAGA IPVMCLPGHR AAELGHFAAV SAATGLVVAD VASGFDYRPM ARELVADHPT LRHVIVDGDP GPFVSWAQLC AQAGTGSPAP PADPGSPALL LVSGGTTGMP KLIPRTHDDY VFNATASAAL CRLSADDVYL VVLAAGHNFP LACPGLLGAM TVGATAVFAP DPSPEAAFAA IERHGVTVTA LVPALAKLWA QSCEWEPVTP KSLRLLQVGG SKLEPEDARR VRTALTPGLQ QVFGMAEGLL NFTRIGDPPE VVEHTQGRPL CPADELRIVN ADGEPVGPGE EGELLVRGPY TLNGYFAAER DNERCFDPDG FYRSGDLVRR RDDGNLVVTG RVKDVICRAG ETIAASDLEE QLLSHPAIFS AAAVGLPDQY LGEKICAAVV FAGAPITLAE LNGYLDRRGV AAHTRPDQLV AMPALPTTPI GKIDKRAIVR QLGIA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catggctttg taccgttccc cgcggatcgg gccgcgtcgt accgggcggc cggctattgg tcggggcgaa ccctggacac cgtgctctcc gatgccgcgc ggcgctggcc tgaccgcctc gcggtggccg acgccggtga tcgtcccggc cacggcggcc tcagttacgc cgaactcgac cagcgggccg accgggccgc cgcggcgctg cacggcctgg gcatcacgcc aggcgaccgg gtactgctcc agctgccaaa cggctgccag ttcgcggttg ccctgttcgc gttattgcgg gcgggagcga tcccagtgat gtgcctgccc ggtcaccgcg ccgccgaatt gggccacttc gccgccgtca gcgcggccac cgggctggtg gtcgccgatg tggccagcgg gttcgactat cggccgatgg cgcgcgaact tgttgccgat caccccaccc tgcgccatgt catcgtcgat ggcgatccgg gaccgttcgt gtcgtgggcg cagctgtgcg cccaggccgg caccggttcg ccggcaccgc cggccgatcc cggatcgcca gcgctgctgc tggtctccgg cggcaccact ggcatgccca aactcattcc acgcacccac gacgactacg tgttcaacgc gacggccagc gccgcactct gtcggcttag cgccgacgac gtctatctgg tggtgctggc cgccggccac aatttcccgc tggcctgccc gggcctgctc ggcgcgatga ccgtcggggc caccgccgtg ttcgcccccg atcccagccc ggaggccgcc ttcgccgcca tcgagcgcca cggtgtcacc gtcaccgcgt tggttccggc actggccaaa ctgtgggccc aatcctgtga gtgggagccg gtgacaccga agtcactgcg gttgttgcag gttggcgggt ccaagctaga acccgaggac gctcgccggg tacgcaccgc gctcaccccg ggcctgcagc aggtgttcgg catggcggag gggctgctga acttcacccg catcggcgac ccacccgaag tggtggagca cacccagggg cggccactat gcccggccga cgaactgcgc atcgtcaacg ccgatggtga gccggtgggg cccggggagg aaggcgaact cttggtgcgc gggccctaca cgctgaacgg ctattttgct gccgaacgcg acaacgagcg ctgcttcgat ccggacggct tctaccgcag cggcgacctg gtccgccgcc gcgacgacgg caatctggtg gtcaccgggc gcgtcaagga tgtcatctgc cgtgcgggag aaaccatcgc cgccagcgac ctcgaagaac agctgctgag ccatccggcg atcttctcgg ccgcggcggt gggactacct gaccagtatc tgggggaaaa aatctgcgct gcagtcgttt tcgctggagc tccgattacg cttgcggagt tgaacggcta ccttgaccgg cgtggtgtgg ccgcgcatac gcgacccgac cagctggtcg cgatgccggc gctgcccaca acgccgatcg ggaagatcga caaacgagcg atcgtccgcc agctcggcat cgcgtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat