MytuD.00629.b

PROBABLE FATTY-ACID-CoA LIGASE FADD7 (FATTY-ACID-CoA SYNTHETASE) (FATTY-ACID-CoA SYNTHASE)

CENTER ID: MytuD.00629.b
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.00629.b.A1.GE32586 Full length( MytuD.00629.b ) 1 525
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.134
RefSeq: NP_214633.1
TubercuList: Rv0119
MTB Network Portal: Rv0119
UniProt: O07169

Orthologues for Rv0119

Species % Identity % Coverage Clones Proteins Structures
fortuitum 31 80 0 0 0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MASDFGPRIA DLVEVAATRL PEAPALVVTA DRIAISHRDL ARLVDELAGQ LTRSGLLPGD RVALRMGSNA EFVVALLAAS RADLVVVPLD PALPITEQRV RSQAAGARVV LIDADGPHDR AEPTTRWWPL TVNVGGDSGP SGGTLSVHLD AATEPNPATS TPEGLRPDDA MIMFTGGTTG LPKMVPWTHA NIASSVRAII TGYRLSPRDA TVAVMPLYHG HGLIASLLAT LASGGAVSLP ARGRFSAHTF WDDIKAVGAT WYTAVPTIHQ ILLERSATEP SGRKPAALRF IRSCSAPLTA QAALALQTEF AAPVVCAFGM TEATHQVTTT QIEGIDQTET PVVSTGLVGR STGAQIRIVG SDGLPLPAGA VGEIWLRGTT VVRGYLGDPT ITAANFTDGW LRTGDLGSLS AAGDLSIRGR IKELINRGGE KISPERVEGV LASHPNVMEA AVFGVPHQLY GEAVAAVIVP RESAPPTREE LVQFCRERLA AFEIPASFQE ASGLPHTAKG SLDRRAVAER FGHSV
NT Sequence
ATGGCATCCG ACTTCGGCCC GCGCATCGCC GATCTTGTCG AGGTGGCGGC GACCCGGCTG CCCGAGGCTC CGGCGCTCGT CGTCACCGCG GATCGCATCG CGATCAGCCA CCGCGACCTG GCCCGTCTGG TTGATGAGCT GGCCGGCCAG CTGACGCGGT CCGGCCTGCT GCCCGGTGAC CGGGTCGCGC TGCGCATGGG CAGCAACGCC GAATTCGTCG TCGCCTTGCT GGCGGCGTCG CGTGCGGATC TCGTCGTCGT GCCGCTGGAT CCGGCGCTGC CCATCACCGA GCAACGCGTC CGAAGCCAGG CCGCGGGAGC CCGGGTGGTG CTGATTGACG CGGATGGGCC GCACGACAGG GCAGAACCCA CCACCCGGTG GTGGCCGCTC ACGGTGAACG TCGGCGGTGA CAGCGGCCCC TCGGGTGGCA CCTTGTCGGT CCACCTGGAC GCCGCCACCG AGCCGAACCC CGCAACCTCG ACGCCCGAGG GACTGCGACC CGATGACGCC ATGATCATGT TCACCGGCGG GACGACCGGC CTGCCGAAGA TGGTCCCCTG GACGCACGCA AACATCGCCA GCTCGGTCCG CGCCATCATC ACCGGGTACC GGCTGAGCCC GCGGGACGCC ACCGTTGCGG TGATGCCGCT CTACCATGGC CACGGGCTGA TCGCGTCGTT GCTTGCCACC CTGGCGTCCG GCGGCGCGGT GTCGCTGCCC GCACGCGGGC GATTCTCCGC GCACACCTTC TGGGACGACA TCAAAGCCGT TGGAGCCACC TGGTATACGG CGGTTCCGAC GATTCACCAA ATCCTGCTGG AGCGATCGGC AACCGAACCG TCGGGGCGCA AACCTGCCGC ACTGCGTTTC ATCCGCAGCT GCAGCGCACC GCTCACTGCC CAAGCCGCGC TAGCACTGCA AACCGAGTTC GCGGCACCGG TCGTGTGTGC CTTCGGCATG ACCGAAGCCA CCCACCAGGT AACGACAACG CAGATTGAGG GTATCGACCA AACCGAAACT CCCGTCGTGT CAACCGGTCT GGTCGGCCGG TCGACGGGAG CGCAAATCCG GATCGTCGGG TCCGACGGGC TGCCACTGCC CGCGGGCGCG GTCGGGGAGA TCTGGCTGCG GGGGACCACC GTGGTACGCG GGTATCTGGG TGACCCGACG ATAACCGCCG CGAATTTCAC CGACGGTTGG TTGCGTACCG GTGATCTCGG GTCCCTGTCG GCGGCCGGTG ACCTGAGCAT CCGCGGCCGC ATCAAGGAAC TCATCAACCG AGGTGGTGAA AAGATCTCGC CCGAGCGCGT CGAGGGCGTG CTGGCCAGCC ATCCAAACGT CATGGAGGCA GCCGTATTCG GCGTCCCGCA CCAGCTCTAC GGCGAGGCGG TCGCGGCGGT GATTGTGCCT CGTGAGTCCG CCCCGCCGAC TCGCGAGGAG CTTGTCCAGT TCTGCCGGGA ACGGTTGGCG GCCTTCGAGA TCCCGGCCTC CTTCCAGGAG GCCAGCGGGC TGCCGCACAC CGCGAAGGGT TCGCTCGACC GCCGCGCTGT CGCCGAACGG TTCGGCCATT CGGTG
Details for MytuD.00629.b.A1.GE32586
HARVESTED ON: 5/17/2011
SEQUENCED ON: 5/23/2011
EXPECTED MW: 57kDa
OBSERVED MW: 57kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 95
Validated AA Sequence
MASDFGPRIA DLVEVAATRL PEAPALVVTA DRIAISHRDL ARLVDELAGQ LTRSGLLPGD RVALRMGSNA EFVVALLAAS RADLVVVPLD PALPITEQRV RSQAAGARVV LIDADGPHDR AEPTTRWWPL TVNVGGDSGP SGGTLSVHLD AATEPNPATS TPEGLRPDDA MIMFTGGTTG LPKMVPWTHA NIASSVRAII TGYRLSPRDA TVAVMPLYHG HGLIASLLAT LASGGAVSLP ARGRFSAHTF WDDIKAVGAT WYTAVPTIHQ ILLERSATEP SGRKPAALRF IRSCSAPLTA QAALALQTEF AAPVVCAFGM TEATHQVTTT QIEGIDQTET PVVSTGLVGR STGAQIRIVG SDGLPLPAGA VGEIWLRGTT VVRGYLGDPT ITAANFTDGW LRTGDLGSLS AAGDLSIRGR IKELINRGGE KISPERVEGV LASHPNVMEA AVFGVPHQLY GEAVAAVIVP RESAPPTREE LVQFCRERLA AFEIPASFQE ASGLPHTAKG SLDRRAVAER FGHS
Validated NT Sequence
ccgaatggcc gaaccgttcg gcgacagcgc ggcggtcgag cgaacccttc gcggtgtgcg gcagcccgct ggcctcctgg aaggaggccg ggatctcgaa ggccgccaac cgttcccggc agaactggac aagctcctcg cgagtcggcg gggcggactc acgaggcaca atcaccgccg cgaccgcctc gccgtagagc tggtgcggga cgccgaatac ggctgcctcc atgacgtttg gatggctggc cagcacgccc tcgacgcgct cgggcgagat cttttcacca cctcggttga tgagttcctt gatgcggccg cggatgctca ggtcaccggc cgccgacagg gacccgagat caccggtacg caaccaaccg tcggtgaaat tcgcggcggt tatcgtcggg tcacccagat acccgcgtac cacggtggtc ccccgcagcc agatctcccc gaccgcgccc gcgggcagtg gcagcccgtc ggacccgacg atccggattt gcgctcccgt cgaccggccg accagaccgg ttgacacgac gggagtttcg gtttggtcga taccctcaat ctgcgttgtc gttacctggt gggtggcttc ggtcatgccg aangcacaca cgaccggtgc cgcgaactcg gtttgcagtg ctagcgcggc ttgggcagtg agcggtgcgc tgcagctgcg gatgaaacgc agtgcggcag gtttgcgccc cgacggttcg gttgccgatc gctccagcag gatttggtga atcgtcggaa ccgccgtata ccaggtggct ccaacggctt tgatgtcgtc ccagaaggtg tgcgcggaga atcgcccgcg tgcgggcagc gacaccgcgc cgccggacgc cagggtggca agcaacgacg cgatcagccc gtggccatgg tagagcggca tcaccgcaac ggtggcgtcc cgcgggctca gccggtaccc ggtgatgatg gcgcggaccg agctggcgat gtttgcgtgc gtccagggga ccatcttcgg caggccggtc gtcccgccgg tgaacatgat catggcgtca tcgggtcgca gtccctcggg cgtcgaggtt gcggggttcg gctcggtggc ggcgtccagg tggaccgaca aggtgccacc cgaggggccg ctgtcaccgc cgacgttcac cgtgagcggc caccaccggg tggtgggttc tgccctgtcg tgcggcccat ccgcgtcaat cagcaccacc cgggctcccg cggcctggct tcggacgcgt tgctcggtga tgggcagcgc cggatccagc ggcacgacga cgagatccgc acgcgacgcc gccagcaagg cgacgacgaa ttcggcgttg ctgcccatgc gcagcgcgac ccggtcaccg ggcagcaggc cggaccgcgt cagctggccg gccagctcat caaccagacg ggccaggtcg cggtggctga tcgcgatgcg atccgcggtg acgacgagcg ccggagcctc gggcagccgg gtcgccgcca cctcgacaag atcggcgatg cgcgggccga agtcggatgc catcgaacca ggaccctggg tctgagcttc cagggtacc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMASDFGPRI ADLVEVAATR LPEAPALVVT ADRIAISHRD LARLVDELAG QLTRSGLLPG DRVALRMGSN AEFVVALLAA SRADLVVVPL DPALPITEQR VRSQAAGARV VLIDADGPHD RAEPTTRWWP LTVNVGGDSG PSGGTLSVHL DAATEPNPAT STPEGLRPDD AMIMFTGGTT GLPKMVPWTH ANIASSVRAI ITGYRLSPRD ATVAVMPLYH GHGLIASLLA TLASGGAVSL PARGRFSAHT FWDDIKAVGA TWYTAVPTIH QILLERSATE PSGRKPAALR FIRSCSAPLT AQAALALQTE FAAPVVCAFG MTEATHQVTT TQIEGIDQTE TPVVSTGLVG RSTGAQIRIV GSDGLPLPAG AVGEIWLRGT TVVRGYLGDP TITAANFTDG WLRTGDLGSL SAAGDLSIRG RIKELINRGG EKISPERVEG VLASHPNVME AAVFGVPHQL YGEAVAAVIV PRESAPPTRE ELVQFCRERL AAFEIPASFQ EASGLPHTAK GSLDRRAVAE RFGHSV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcatccgac ttcggcccgc gcatcgccga tcttgtcgag gtggcggcga cccggctgcc cgaggctccg gcgctcgtcg tcaccgcgga tcgcatcgcg atcagccacc gcgacctggc ccgtctggtt gatgagctgg ccggccagct gacgcggtcc ggcctgctgc ccggtgaccg ggtcgcgctg cgcatgggca gcaacgccga attcgtcgtc gccttgctgg cggcgtcgcg tgcggatctc gtcgtcgtgc cgctggatcc ggcgctgccc atcaccgagc aacgcgtccg aagccaggcc gcgggagccc gggtggtgct gattgacgcg gatgggccgc acgacagggc agaacccacc acccggtggt ggccgctcac ggtgaacgtc ggcggtgaca gcggcccctc gggtggcacc ttgtcggtcc acctggacgc cgccaccgag ccgaaccccg caacctcgac gcccgaggga ctgcgacccg atgacgccat gatcatgttc accggcggga cgaccggcct gccgaagatg gtcccctgga cgcacgcaaa catcgccagc tcggtccgcg ccatcatcac cgggtaccgg ctgagcccgc gggacgccac cgttgcggtg atgccgctct accatggcca cgggctgatc gcgtcgttgc ttgccaccct ggcgtccggc ggcgcggtgt cgctgcccgc acgcgggcga ttctccgcgc acaccttctg ggacgacatc aaagccgttg gagccacctg gtatacggcg gttccgacga ttcaccaaat cctgctggag cgatcggcaa ccgaaccgtc ggggcgcaaa cctgccgcac tgcgtttcat ccgcagctgc agcgcaccgc tcactgccca agccgcgcta gcactgcaaa ccgagttcgc ggcaccggtc gtgtgtgcct tcggcatgac cgaagccacc caccaggtaa cgacaacgca gattgagggt atcgaccaaa ccgaaactcc cgtcgtgtca accggtctgg tcggccggtc gacgggagcg caaatccgga tcgtcgggtc cgacgggctg ccactgcccg cgggcgcggt cggggagatc tggctgcggg ggaccaccgt ggtacgcggg tatctgggtg acccgacgat aaccgccgcg aatttcaccg acggttggtt gcgtaccggt gatctcgggt ccctgtcggc ggccggtgac ctgagcatcc gcggccgcat caaggaactc atcaaccgag gtggtgaaaa gatctcgccc gagcgcgtcg agggcgtgct ggccagccat ccaaacgtca tggaggcagc cgtattcggc gtcccgcacc agctctacgg cgaggcggtc gcggcggtga ttgtgcctcg tgagtccgcc ccgccgactc gcgaggagct tgtccagttc tgccgggaac ggttggcggc cttcgagatc ccggcctcct tccaggaggc cagcgggctg ccgcacaccg cgaagggttc gctcgaccgc cgcgctgtcg ccgaacggtt cggccattcg gtgtaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa