MytuD.00609.b

PROBABLE GLUTAMINE-TRANSPORT ATP-BINDING PROTEIN ABC TRANSPORTER GLNQ

CENTER ID: MytuD.00609.b
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.00609.b.B1.GE27164 Full length( MytuD.00609.b ) 1 330
MytuD.00609.b.B1.GE27585 Full length( MytuD.00609.b ) 1 330
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.2872
RefSeq: NP_217080.1
TubercuList: Rv2564
MTB Network Portal: Rv2564
UniProt: P9WQI5

Orthologues for Rv2564

Species % Identity % Coverage Clones Proteins Structures
avium 47 67 0 0 0
paratuberculosis 46 67 0 0 0
smegmatis 42 62 0 0 0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGGLTISDLV VEYSSGGYAV RPIDGLSLDV APGSLVILLG PSGCGKTTLL SCLGGILRPK SGSIKFDDVD ITTLEGAALA KYRRDKVGIV FQAFNLVSSL TALENVMVPL RAAGVSRAAA RKRAEDLLIR VNLGERMKHR PGDMSGGQQQ RVAVARAIAL DPQLILADEP TAHLDFIQVE EVLRLIRSLA QGDRVVVVAT HDSRMLPLAD RVLELMPAQV SPNQPPETVH VKAGEVLFEQ STMGDLIYVV SEGEFEIVRE LADGGEELVK TAAPGDYFGE IGVLFHLPRS ATVRARSDAT AVGYTAQAFR ERLGVTRVAD LIEHRELASE
NT Sequence
ATGGGCGGCC TAACCATTTC CGACCTGGTC GTCGAGTATT CCAGCGGCGG GTACGCCGTG CGGCCGATCG ACGGGTTAAG CCTCGACGTG GCGCCGGGGT CGCTGGTGAT CTTGCTTGGG CCCAGCGGCT GCGGGAAGAC GACCCTCTTG TCCTGCCTCG GCGGCATCCT GCGCCCGAAG TCCGGCTCAA TCAAGTTTGA CGATGTCGAC ATCACGACGC TGGAGGGCGC CGCGCTGGCG AAGTATCGGC GTGACAAGGT AGGGATCGTC TTCCAGGCGT TCAACCTGGT CTCGAGCCTT ACCGCCCTGG AGAACGTGAT GGTCCCGCTG CGCGCGGCCG GCGTGTCACG AGCGGCCGCG CGTAAGCGTG CCGAGGACCT GCTGATCCGA GTCAATCTCG GCGAACGAAT GAAACACCGC CCGGGTGACA TGAGCGGCGG CCAGCAGCAA CGCGTCGCGG TCGCCCGCGC GATCGCGCTG GACCCGCAAT TGATCCTTGC CGACGAACCG ACCGCGCACC TGGACTTCAT CCAGGTGGAG GAGGTGCTGC GGCTGATCCG CTCGCTAGCG CAGGGCGACC GTGTGGTGGT GGTCGCGACC CACGACAGCC GGATGCTGCC GCTGGCCGAT CGCGTCCTTG AGCTGATGCC GGCGCAGGTG TCGCCGAATC AGCCACCCGA AACGGTGCAC GTGAAAGCCG GCGAGGTGCT GTTCGAGCAG TCCACAATGG GCGATCTGAT CTACGTGGTG TCCGAGGGCG AGTTCGAGAT TGTGCGCGAA TTGGCCGACG GCGGTGAGGA ATTGGTCAAA ACCGCCGCGC CTGGGGACTA CTTCGGTGAA ATCGGCGTGC TGTTTCACCT GCCACGCTCG GCAACGGTAC GGGCTCGCAG CGACGCGACA GCCGTCGGTT ATACGGCGCA GGCGTTTCGG GAGCGGCTGG GTGTGACGCG GGTGGCCGAC CTGATTGAGC ACCGCGAGCT TGCCAGCGAA
Details for MytuD.00609.b.B1.GE27164
HARVESTED ON: 7/20/2009
SEQUENCED ON: 7/28/2009
EXPECTED MW: 36kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 99
PERCENT COVERAGE: 97
Validated AA Sequence
MGGLTISDLV VEYSSGGYAV RPIDGLSLDV APGSLVILLG PSGCGKTTLL SCLGGILRPK SGSIKFDDVD ITTLEGAALA KYRRDKVGIV FQAFNLVSSL TALENVMVPL RAAGVSRAAA RKRAEDLLIR VNLGERMKHR PGDMSGGQQQ RVAVARAIAL DPQLILADEP TAHLDFIQVE EVLRLIRSLA QGDRVVVVAT HDSRMLPLAD RVLEPMPAQV SPNQPPETVH VKAGEVLFEQ STMGDLIYVV SEGEFEIVRE LADGGEELVK TAAPGDYFGE IGVLFHLPRS ATVRARSDAT AVGYTAQAFR ERLGVTRVAD LIEHRELASE
Validated NT Sequence
ttaagaagga gnnataccnt ggctcaccac caccaccacc atatgggcgg cctaaccatt tccgacctgg tcgtcgagta ttccagcggc gggtacgccg tgcggccgat cgacgggtta agcctcgacg tggcgccggg gtcgctggtg atcttgcttg ggcccagcgg ctgcgggaag acgaccctct tgtcctgcct cggcggcatc ctgcgcccga agtccggctc aatcaagttt gacgatgtcg acatcacgac gctggagggc gccgcgctgg cgaagtatcg gcgtgacaag gtagggatcg tcttccaggc gttcaacctg gtctcgagcc ttaccgccct ggagaacgtg atggtcccgc tgcgcgcggc cggcgtgtca cgagcggccg cgcgtaagcg tgccgaggac ctgctgatcc gagtcaatct cggcgaacga atgaaacacc gcccgggtga catgagcggc ggccagcagc aacgcgtcgc ggtcgcccgc gcgatcgcgc tggacccgca attgatcctt gccgacgaac cgaccgcgca cctggacttc atccaggtgg aggaggtgct gcggctgatc cgctcgctag cgcagggcga ccgtgtggtg gtggtcgcga cccacgacag ccggatgctg ccgctggccg atcgcgtcct tgagccgatg ccggcgcagg tgtcgccgaa tcagccaccc gaaacggtgc acgtgaaagc cggcgaggtg ctgttcgagc agtccacaat gggcgatctg atctacgtgg tgtccgaggg cgagttcgag attgtgcgcg aattggccga cggcggtgag gaattggtca aaaccgccgc gcctggggac tacttcggtg aaatcggcgt gctgtttcac ctgccacgct cggcaacggt acgggctcgc agcgacgcga cagccgtcgg ttatacggcg caggcgtttc gggagcggct gggtgtgacg cgggtggccg acctgattga gcaccgcgag cttgccagcg aataagtgag ta
Expected Protein Sequence
MAHHHHHHMG GLTISDLVVE YSSGGYAVRP IDGLSLDVAP GSLVILLGPS GCGKTTLLSC LGGILRPKSG SIKFDDVDIT TLEGAALAKY RRDKVGIVFQ AFNLVSSLTA LENVMVPLRA AGVSRAAARK RAEDLLIRVN LGERMKHRPG DMSGGQQQRV AVARAIALDP QLILADEPTA HLDFIQVEEV LRLIRSLAQG DRVVVVATHD SRMLPLADRV LELMPAQVSP NQPPETVHVK AGEVLFEQST MGDLIYVVSE GEFEIVRELA DGGEELVKTA APGDYFGEIG VLFHLPRSAT VRARSDATAV GYTAQAFRER LGVTRVADLI EHRELASE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggcg gcctaaccat ttccgacctg gtcgtcgagt attccagcgg cgggtacgcc gtgcggccga tcgacgggtt aagcctcgac gtggcgccgg ggtcgctggt gatcttgctt gggcccagcg gctgcgggaa gacgaccctc ttgtcctgcc tcggcggcat cctgcgcccg aagtccggct caatcaagtt tgacgatgtc gacatcacga cgctggaggg cgccgcgctg gcgaagtatc ggcgtgacaa ggtagggatc gtcttccagg cgttcaacct ggtctcgagc cttaccgccc tggagaacgt gatggtcccg ctgcgcgcgg ccggcgtgtc acgagcggcc gcgcgtaagc gtgccgagga cctgctgatc cgagtcaatc tcggcgaacg aatgaaacac cgcccgggtg acatgagcgg cggccagcag caacgcgtcg cggtcgcccg cgcgatcgcg ctggacccgc aattgatcct tgccgacgaa ccgaccgcgc acctggactt catccaggtg gaggaggtgc tgcggctgat ccgctcgcta gcgcagggcg accgtgtggt ggtggtcgcg acccacgaca gccggatgct gccgctggcc gatcgcgtcc ttgagctgat gccggcgcag gtgtcgccga atcagccacc cgaaacggtg cacgtgaaag ccggcgaggt gctgttcgag cagtccacaa tgggcgatct gatctacgtg gtgtccgagg gcgagttcga gattgtgcgc gaattggccg acggcggtga ggaattggtc aaaaccgccg cgcctgggga ctacttcggt gaaatcggcg tgctgtttca cctgccacgc tcggcaacgg tacgggctcg cagcgacgcg acagccgtcg gttatacggc gcaggcgttt cgggagcggc tgggtgtgac gcgggtggcc gacctgattg agcaccgcga gcttgccagc gaatgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for MytuD.00609.b.B1.GE27585
HARVESTED ON: 8/17/2009
SEQUENCED ON: 9/29/2009
EXPECTED MW: 36kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 99
PERCENT COVERAGE: 97
Validated AA Sequence
MGGLTISDLV VEYSSGGYAV RPIDGLSLDV APGSLVILLG PSGCGKTTLL SCLGGILRPK SGSIKFDDVD ITTLEGAALA KYRRDKVGIV FQAFNLVSSL TALENVMVPL RAAGVSRAAA RKRAEDLLIR VNLGERMKHR PGDMSGGQQQ RVAVARAIAL DPQLILADEP TAHLDFIQVE EVLRLIRSLA QGDRVVVVAT HDSRMLPLAD RVLEPMPAQV SPNQPPETVH VKAGEVLFEQ STMGDLIYVV SEGEFEIVRE LADGGEELVK TAAPGDYFGE IGVLFHLPRS ATVRARSDAT AVGYTAQAFR ERLGVTRVAD LIEHRELASE
Validated NT Sequence
ttaagaagga gnnataccnt ggctcaccac caccaccacc atatgggcgg cctaaccatt tccgacctgg tcgtcgagta ttccagcggc gggtacgccg tgcggccgat cgacgggtta agcctcgacg tggcgccggg gtcgctggtg atcttgcttg ggcccagcgg ctgcgggaag acgaccctct tgtcctgcct cggcggcatc ctgcgcccga agtccggctc aatcaagttt gacgatgtcg acatcacgac gctggagggc gccgcgctgg cgaagtatcg gcgtgacaag gtagggatcg tcttccaggc gttcaacctg gtctcgagcc ttaccgccct ggagaacgtg atggtcccgc tgcgcgcggc cggcgtgtca cgagcggccg cgcgtaagcg tgccgaggac ctgctgatcc gagtcaatct cggcgaacga atgaaacacc gcccgggtga catgagcggc ggccagcagc aacgcgtcgc ggtcgcccgc gcgatcgcgc tggacccgca attgatcctt gccgacgaac cgaccgcgca cctggacttc atccaggtgg aggaggtgct gcggctgatc cgctcgctag cgcagggcga ccgtgtggtg gtggtcgcga cccacgacag ccggatgctg ccgctggccg atcgcgtcct tgagccgatg ccggcgcagg tgtcgccgaa tcagccaccc gaaacggtgc acgtgaaagc cggcgaggtg ctgttcgagc agtccacaat gggcgatctg atctacgtgg tgtccgaggg cgagttcgag attgtgcgcg aattggccga cggcggtgag gaattggtca aaaccgccgc gcctggggac tacttcggtg aaatcggcgt gctgtttcac ctgccacgct cggcaacggt acgggctcgc agcgacgcga cagccgtcgg ttatacggcg caggcgtttc gggagcggct gggtgtgacg cgggtggccg acctgattga gcaccgcgag cttgccagcg aataagtgag ta
Expected Protein Sequence
MAHHHHHHMG GLTISDLVVE YSSGGYAVRP IDGLSLDVAP GSLVILLGPS GCGKTTLLSC LGGILRPKSG SIKFDDVDIT TLEGAALAKY RRDKVGIVFQ AFNLVSSLTA LENVMVPLRA AGVSRAAARK RAEDLLIRVN LGERMKHRPG DMSGGQQQRV AVARAIALDP QLILADEPTA HLDFIQVEEV LRLIRSLAQG DRVVVVATHD SRMLPLADRV LELMPAQVSP NQPPETVHVK AGEVLFEQST MGDLIYVVSE GEFEIVRELA DGGEELVKTA APGDYFGEIG VLFHLPRSAT VRARSDATAV GYTAQAFRER LGVTRVADLI EHRELASE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggcg gcctaaccat ttccgacctg gtcgtcgagt attccagcgg cgggtacgcc gtgcggccga tcgacgggtt aagcctcgac gtggcgccgg ggtcgctggt gatcttgctt gggcccagcg gctgcgggaa gacgaccctc ttgtcctgcc tcggcggcat cctgcgcccg aagtccggct caatcaagtt tgacgatgtc gacatcacga cgctggaggg cgccgcgctg gcgaagtatc ggcgtgacaa ggtagggatc gtcttccagg cgttcaacct ggtctcgagc cttaccgccc tggagaacgt gatggtcccg ctgcgcgcgg ccggcgtgtc acgagcggcc gcgcgtaagc gtgccgagga cctgctgatc cgagtcaatc tcggcgaacg aatgaaacac cgcccgggtg acatgagcgg cggccagcag caacgcgtcg cggtcgcccg cgcgatcgcg ctggacccgc aattgatcct tgccgacgaa ccgaccgcgc acctggactt catccaggtg gaggaggtgc tgcggctgat ccgctcgcta gcgcagggcg accgtgtggt ggtggtcgcg acccacgaca gccggatgct gccgctggcc gatcgcgtcc ttgagctgat gccggcgcag gtgtcgccga atcagccacc cgaaacggtg cacgtgaaag ccggcgaggt gctgttcgag cagtccacaa tgggcgatct gatctacgtg gtgtccgagg gcgagttcga gattgtgcgc gaattggccg acggcggtga ggaattggtc aaaaccgccg cgcctgggga ctacttcggt gaaatcggcg tgctgtttca cctgccacgc tcggcaacgg tacgggctcg cagcgacgcg acagccgtcg gttatacggc gcaggcgttt cgggagcggc tgggtgtgac gcgggtggcc gacctgattg agcaccgcga gcttgccagc gaatgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat