MytuD.00560.a

PROBABLE PROLINE IMINOPEPTIDASE PIP (PROLYL AMINOPEPTIDASE) (PAP)

CENTER ID: MytuD.00560.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.00560.a.A1.GE25855 Full length( MytuD.00560.a ) 1 286
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.00560.a.A1.PS00174 Full length( MytuD.00560.a ) 1 286

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.940
RefSeq: NP_215355.1
TubercuList: Rv0840c
MTB Network Portal: Rv0840c
UniProt: I6Y8X0

Orthologues for Rv0840c

Species % Identity % Coverage Clones Proteins Structures
marinum 75 99 1 1 0
ulcerans 73 90 1 0 0
paratuberculosis 72 99 0 0 0
avium 72 98 0 0 0
smegmatis 39 92 1 1 1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEGTIAVPGG RVWFQRIGGG PGRPLLVVHG GPGLPHNYLA PLRRLSDERE VIFWDQLGCG NSACPSDVDL WTMNRSVAEM ATVAEALALT RFHIFSHSWG GMLAQQYVLD KAPDAVSLTI ANSTASIPEF SASLVSLKSC LDVATRSAID RHEAAGTTHS AEYQAAIRTW NETYLCRTRP WPRELTEAFA NMGTEIFETM FGPSDFRIVG NVRDWDVVDR LADIAVPTLL VVGRFDECSP EHMREMQGRI AGSRLEFFES SSHMPFIEEP ARFDRVMREF LRLHDI
NT Sequence
GTGGAGGGGA CAATCGCGGT CCCGGGTGGA CGCGTCTGGT TCCAGCGGAT TGGTGGCGGT CCTGGTCGTC CGCTGCTTGT AGTGCACGGT GGGCCGGGCT TGCCGCACAA CTACTTGGCC CCACTGCGAC GGTTGTCTGA TGAGCGGGAG GTCATCTTCT GGGACCAGCT CGGTTGCGGA AATTCCGCAT GTCCGTCAGA CGTAGACCTT TGGACGATGA ACCGCTCAGT GGCCGAGATG GCAACCGTGG CGGAAGCCCT TGCCCTTACC CGCTTTCACA TCTTCAGCCA TTCGTGGGGT GGGATGCTGG CACAGCAGTA CGTGCTCGAC AAGGCGCCTG ACGCCGTCAG TCTGACCATC GCGAACAGCA CGGCTTCGAT ACCCGAATTT TCGGCCAGTC TGGTCAGCTT GAAGTCGTGC TTGGACGTGG CAACTCGCTC GGCAATTGAC CGTCACGAGG CGGCCGGCAC CACCCATTCC GCCGAATACC AGGCCGCGAT CAGAACCTGG AACGAGACTT ATCTGTGCCG CACCCGCCCC TGGCCCCGGG AACTCACGGA AGCATTCGCC AACATGGGAA CCGAGATCTT CGAGACGATG TTTGGGCCCA GCGACTTTCG CATCGTTGGG AATGTTCGAG ACTGGGACGT CGTCGACCGG TTGGCCGACA TCGCGGTGCC GACCTTGCTG GTGGTGGGCC GTTTCGACGA ATGTTCGCCT GAGCACATGC GAGAAATGCA GGGCCGGATT GCGGGCTCGC GATTGGAATT CTTCGAGTCC AGTTCCCACA TGCCGTTCAT CGAAGAGCCG GCGCGATTCG ACCGGGTGAT GCGTGAATTC CTTCGGCTGC ACGATATT
Details for MytuD.00560.a.A1.GE25855
HARVESTED ON: 11/19/2008
SEQUENCED ON: 3/10/2015
EXPECTED MW: 34kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVEGTIAVP GGRVWFQRIG GGPGRPLLVV HGGPGLPHNY LAPLRRLSDE REVIFWDQLG CGNSACPSDV DLWTMNRSVA EMATVAEALA LTRFHIFSHS WGGMLAQQYV LDKAPDAVSL TIANSTASIP EFSASLVSLK SCLDVATRSA IDRHEAAGTT HSAEYQAAIR TWNETYLCRT RPWPRELTEA FANMGTEIFE TMFGPSDFRI VGNVRDWDVV DRLADIAVPT LLVVGRFDEC SPEHMREMQG RIAGSRLEFF ESSSHMPFIE EPARFDRVMR EFLR
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtgg aggggacaat cgcggtcccg ggtggacgcg tctggttcca gcggattggt ggcggtcctg gtcgtccgct gcttgtagtg cacggtgggc cgggcttgcc gcacaactac ttggccccac tgcgacggtt gtctgatgag cgggaggtca tcttctggga ccagctcggt tgcggaaatt ccgcatgtcc gtcagacgta gacctttgga cgatgaaccg ctcagtggcc gagatggcaa ccgtggcgga agcccttgcc cttacccgct ttcacatctt cagccattcg tggggtggga tgctggcaca gcagtacgtg ctcgacaagg cgcctgacgc cgtcagtctg accatcgcga acagcacggc ttcgataccc gaattttcgg ccagtctggt cagcttgaag tcgtgcttgg acgtggcaac tcgctcggca attgaccgtc acgaggcggc cggcaccacc cattccgccg aataccaggc cgcgatcaga acctggaacg agacttatct gtgccgcacc cgcccctggc cccgggaact cacggaagca ttcgccaaca tgggaaccga gatcttcgag acgatgtttg ggcccagcga ctttcgcatc gttgggaatg ttcgagactg ggacgtcgtc gaccggttgg ccgacatcgc ggtgccgacc ttgctggtgg tgggccgttt cgacgaatgt tcgcctgagc acatgcgaga aatgcagggc cggattgcgg gctcgcgatt ggaattcttc gagtccagtt cccacatgcc gttcatcgaa gagccggcgc gattcgaccg ggtgatgcgt gaattccttc ggctg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVEGTIAVP GGRVWFQRIG GGPGRPLLVV HGGPGLPHNY LAPLRRLSDE REVIFWDQLG CGNSACPSDV DLWTMNRSVA EMATVAEALA LTRFHIFSHS WGGMLAQQYV LDKAPDAVSL TIANSTASIP EFSASLVSLK SCLDVATRSA IDRHEAAGTT HSAEYQAAIR TWNETYLCRT RPWPRELTEA FANMGTEIFE TMFGPSDFRI VGNVRDWDVV DRLADIAVPT LLVVGRFDEC SPEHMREMQG RIAGSRLEFF ESSSHMPFIE EPARFDRVMR EFLRLHDI
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtggagggg acaatcgcgg tcccgggtgg acgcgtctgg ttccagcgga ttggtggcgg tcctggtcgt ccgctgcttg tagtgcacgg tgggccgggc ttgccgcaca actacttggc cccactgcga cggttgtctg atgagcggga ggtcatcttc tgggaccagc tcggttgcgg aaattccgca tgtccgtcag acgtagacct ttggacgatg aaccgctcag tggccgagat ggcaaccgtg gcggaagccc ttgcccttac ccgctttcac atcttcagcc attcgtgggg tgggatgctg gcacagcagt acgtgctcga caaggcgcct gacgccgtca gtctgaccat cgcgaacagc acggcttcga tacccgaatt ttcggccagt ctggtcagct tgaagtcgtg cttggacgtg gcaactcgct cggcaattga ccgtcacgag gcggccggca ccacccattc cgccgaatac caggccgcga tcagaacctg gaacgagact tatctgtgcc gcacccgccc ctggccccgg gaactcacgg aagcattcgc caacatggga accgagatct tcgagacgat gtttgggccc agcgactttc gcatcgttgg gaatgttcga gactgggacg tcgtcgaccg gttggccgac atcgcggtgc cgaccttgct ggtggtgggc cgtttcgacg aatgttcgcc tgagcacatg cgagaaatgc agggccggat tgcgggctcg cgattggaat tcttcgagtc cagttcccac atgccgttca tcgaagagcc ggcgcgattc gaccgggtga tgcgtgaatt ccttcggctg cacgatatta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for MytuD.00560.a.A1.PS00174
PURIFICATION DATe: 2/27/2009
CONCENTRATION: 22.58mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 13
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVEGTIAVP GGRVWFQRIG GGPGRPLLVV HGGPGLPHNY LAPLRRLSDE REVIFWDQLG CGNSACPSDV DLWTMNRSVA EMATVAEALA LTRFHIFSHS WGGMLAQQYV LDKAPDAVSL TIANSTASIP EFSASLVSLK SCLDVATRSA IDRHEAAGTT HSAEYQAAIR TWNETYLCRT RPWPRELTEA FANMGTEIFE TMFGPSDFRI VGNVRDWDVV DRLADIAVPT LLVVGRFDEC SPEHMREMQG RIAGSRLEFF ESSSHMPFIE EPARFDRVMR EFLR
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtgg aggggacaat cgcggtcccg ggtggacgcg tctggttcca gcggattggt ggcggtcctg gtcgtccgct gcttgtagtg cacggtgggc cgggcttgcc gcacaactac ttggccccac tgcgacggtt gtctgatgag cgggaggtca tcttctggga ccagctcggt tgcggaaatt ccgcatgtcc gtcagacgta gacctttgga cgatgaaccg ctcagtggcc gagatggcaa ccgtggcgga agcccttgcc cttacccgct ttcacatctt cagccattcg tggggtggga tgctggcaca gcagtacgtg ctcgacaagg cgcctgacgc cgtcagtctg accatcgcga acagcacggc ttcgataccc gaattttcgg ccagtctggt cagcttgaag tcgtgcttgg acgtggcaac tcgctcggca attgaccgtc acgaggcggc cggcaccacc cattccgccg aataccaggc cgcgatcaga acctggaacg agacttatct gtgccgcacc cgcccctggc cccgggaact cacggaagca ttcgccaaca tgggaaccga gatcttcgag acgatgtttg ggcccagcga ctttcgcatc gttgggaatg ttcgagactg ggacgtcgtc gaccggttgg ccgacatcgc ggtgccgacc ttgctggtgg tgggccgttt cgacgaatgt tcgcctgagc acatgcgaga aatgcagggc cggattgcgg gctcgcgatt ggaattcttc gagtccagtt cccacatgcc gttcatcgaa gagccggcgc gattcgaccg ggtgatgcgt gaattccttc ggctg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVEGTIAVP GGRVWFQRIG GGPGRPLLVV HGGPGLPHNY LAPLRRLSDE REVIFWDQLG CGNSACPSDV DLWTMNRSVA EMATVAEALA LTRFHIFSHS WGGMLAQQYV LDKAPDAVSL TIANSTASIP EFSASLVSLK SCLDVATRSA IDRHEAAGTT HSAEYQAAIR TWNETYLCRT RPWPRELTEA FANMGTEIFE TMFGPSDFRI VGNVRDWDVV DRLADIAVPT LLVVGRFDEC SPEHMREMQG RIAGSRLEFF ESSSHMPFIE EPARFDRVMR EFLRLHDI
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGGAGGGG ACAATCGCGG TCCCGGGTGG ACGCGTCTGG TTCCAGCGGA TTGGTGGCGG TCCTGGTCGT CCGCTGCTTG TAGTGCACGG TGGGCCGGGC TTGCCGCACA ACTACTTGGC CCCACTGCGA CGGTTGTCTG ATGAGCGGGA GGTCATCTTC TGGGACCAGC TCGGTTGCGG AAATTCCGCA TGTCCGTCAG ACGTAGACCT TTGGACGATG AACCGCTCAG TGGCCGAGAT GGCAACCGTG GCGGAAGCCC TTGCCCTTAC CCGCTTTCAC ATCTTCAGCC ATTCGTGGGG TGGGATGCTG GCACAGCAGT ACGTGCTCGA CAAGGCGCCT GACGCCGTCA GTCTGACCAT CGCGAACAGC ACGGCTTCGA TACCCGAATT TTCGGCCAGT CTGGTCAGCT TGAAGTCGTG CTTGGACGTG GCAACTCGCT CGGCAATTGA CCGTCACGAG GCGGCCGGCA CCACCCATTC CGCCGAATAC CAGGCCGCGA TCAGAACCTG GAACGAGACT TATCTGTGCC GCACCCGCCC CTGGCCCCGG GAACTCACGG AAGCATTCGC CAACATGGGA ACCGAGATCT TCGAGACGAT GTTTGGGCCC AGCGACTTTC GCATCGTTGG GAATGTTCGA GACTGGGACG TCGTCGACCG GTTGGCCGAC ATCGCGGTGC CGACCTTGCT GGTGGTGGGC CGTTTCGACG AATGTTCGCC TGAGCACATG CGAGAAATGC AGGGCCGGAT TGCGGGCTCG CGATTGGAAT TCTTCGAGTC CAGTTCCCAC ATGCCGTTCA TCGAAGAGCC GGCGCGATTC GACCGGGTGA TGCGTGAATT CCTTCGGCTG CACGATATTA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A