MytuD.00541.a

succinyl-CoA synthetase subunit beta

CENTER ID: MytuD.00541.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.00541.a.B1.GE27163 Full length( MytuD.00541.a ) 1 387
MytuD.00541.a.B1.GE27584 Full length( MytuD.00541.a ) 1 387
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.1060
RefSeq: NP_215466.1
TubercuList: Rv0951
MTB Network Portal: Rv0951
UniProt: P9WGC5

Orthologues for Rv0951

Species % Identity % Coverage Clones Proteins Structures
marinum 93 100 1 0 0
ulcerans 92 100 1 0 0
avium 90 100 0 0 0
paratuberculosis 89 100 0 0 0
smegmatis 89 100 1 0 0
leprae 87 99 1 0 0
abscessus 84 100 0 0 0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDLFEYQAKE LFAKHNVPST PGRVTDTAEG AKAIATEIGR PVMVKAQVKI GGRGKAGGVK YAATPQDAYE HAKNILGLDI KGHIVKKLLV AEASDIAEEY YLSFLLDRAN RTYLAMCSVE GGMEIEEVAA TKPERLAKVP VNAVKGVDLD FARSIAEQGH LPAEVLDTAA VTIAKLWELF VAEDATLVEV NPLVRTPDHK ILALDAKITL DGNADFRQPG HAEFEDRAAT DPLELKAKEH DLNYVKLDGQ VGIIGNGAGL VMSTLDVVAY AGEKHGGVKP ANFLDIGGGA SAEVMAAGLD VVLGDQQVKS VFVNVFGGIT SCDAVATGIV KALGMLGDEA NKPLVVRLDG NNVEEGRRIL TEANHPLVTL VATMDEAADK AAELASA
NT Sequence
ATGGATCTTT TCGAGTATCA AGCCAAGGAG TTATTCGCCA AGCACAACGT GCCCAGCACG CCGGGTCGGG TGACCGACAC AGCCGAGGGT GCCAAGGCTA TCGCCACGGA GATCGGGCGT CCGGTGATGG TCAAAGCGCA GGTCAAGATC GGCGGCCGGG GCAAGGCCGG TGGCGTCAAA TACGCCGCGA CCCCACAAGA CGCGTACGAG CACGCCAAGA ACATCCTCGG CCTGGACATC AAAGGACACA TCGTCAAGAA ACTGCTGGTC GCTGAGGCTA GCGATATCGC CGAGGAGTAC TACCTATCCT TCCTGCTCGA CCGGGCCAAC CGCACCTACC TGGCGATGTG CTCGGTGGAG GGCGGCATGG AGATCGAAGA GGTAGCGGCC ACCAAACCCG AGCGGCTCGC CAAAGTCCCG GTGAATGCCG TCAAGGGCGT TGACCTAGAT TTCGCGCGGT CCATCGCCGA ACAGGGTCAT CTTCCGGCCG AGGTGCTCGA CACCGCAGCG GTCACCATCG CCAAGCTGTG GGAGCTCTTC GTCGCCGAGG ACGCGACGCT GGTTGAGGTC AACCCGTTGG TGCGGACGCC TGACCACAAG ATCCTCGCGC TGGATGCCAA GATCACCCTC GACGGCAACG CCGATTTCCG TCAGCCTGGC CATGCCGAGT TCGAGGATCG AGCTGCCACC GATCCACTGG AGTTGAAGGC CAAGGAGCAC GACCTCAACT ACGTCAAGCT GGACGGTCAG GTGGGGATCA TCGGCAATGG CGCGGGCTTG GTGATGTCGA CTCTCGACGT CGTCGCGTAT GCCGGTGAGA AGCACGGCGG AGTCAAGCCG GCCAACTTCC TGGATATCGG CGGCGGCGCT TCGGCCGAGG TGATGGCCGC GGGTCTGGAC GTGGTGCTGG GCGACCAGCA GGTCAAGAGC GTGTTCGTCA ACGTCTTCGG TGGCATCACC TCGTGCGATG CGGTGGCGAC CGGGATCGTC AAGGCGCTGG GCATGCTGGG TGACGAAGCC AACAAGCCGC TGGTGGTTCG GCTCGACGGC AACAACGTCG AGGAAGGCCG TCGCATCCTG ACCGAGGCCA ACCACCCCCT GGTGACACTG GTGGCGACGA TGGACGAAGC CGCCGACAAG GCCGCTGAGC TGGCGAGCGC C
Details for MytuD.00541.a.B1.GE27163
HARVESTED ON: 7/20/2009
SEQUENCED ON: 7/28/2009
EXPECTED MW: 41kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MDLFEYQAKE LFAKHNVPST PGRVTDTAEG AKAIATEIGR PVMVKAQVKI GGRGKAGGVK YAATPQDAYE HAKNILGLDI KGHIVKKLLV AEASDIAEEY YLSFLLDRAN RTYLAMCSVE GGMEIEEVAA TKPERLAKVP VNAVKGVDLD FARSIAEQGH LPAEVLDTAA VTIAKLWELF VAEDATLVEV NPLVRTPDHK ILALDAKITL DGNADFRQPG HAEFEDRAAT DPLELKAKEH DLNYVKLDGQ VGIIGNGAGL VMSTLDVVAY AGEKHGGVKP ANFLDIGGGA SAEVMAAGLD VVLGDQQVKS VFVNVFGGIT SCDAVATGIV KALGMLGDEA NKPLVVRLDG NNVEEGRRIL TEANHPLVTL VATMDEAADK AAELASA
Validated NT Sequence
tcaccaccac caccaccata tggatctttt cgagtatcaa gccaaggagt tattcgccaa gcacaacgtg cccagcacgc cgggtcgggt gaccgacaca gccgagggtg ccaaggctat cgccacggag atcgggcgtc cggtgatggt caaagcgcag gtcaagatcg gcggccgggg caaggccggt ggcgtcaaat acgccgcgac cccacaagac gcgtacgagc acgccaagaa catcctcggc ctggacatca aaggacacat cgtcaagaaa ctgctggtcg ctgaggctag cgatatcgcc gaggagtact acctatcctt cctgctcgac cgggccaacc gcacctacct ggcgatgtgc tcggtggagg gcggcatgga gatcgaagag gtagcggcca ccaaacccga gcggctcgcc aaagtcccgg tgaatgccgt caagggcgtt gacctagatt tcgcgcggtc catcgccgaa cagggtcatc ttccggccga ggtgctcgac accgcagcgg tcaccatcgc caagctgtgg gagctcttcg tcgccgagga cgcgacgctg gttgaggtca acccgttggt gcggacgcct gaccacaaga tcctcgcgct ggatgccaag atcaccctcg acggcaacgc cgatttccgt cagcctggcc atgccgagtt cgaggatcga gctgccaccg atccactgga gttgaaggcc aaggagcacg acctcaacta cgtcaagctg gacggtcagg tggggatcat cggcaatggc gcgggcttgg tgatgtcgac tctcgacgtc gtcgcgtatg ccggtgagaa gcacggcgga gtcaagccgg ccaacttcct ggatatcggc ggcggcgctt cggccgaggt gatggccgcg ggtctggacg tggtgctggg cgaccagcag gtcaagagcg tgttcgtcaa cgtcttcggt ggcatcacct cgtgcgatgc ggtggcgacc gggatcgtca aggcgctggg catgctgggt gacgaagcca acaagccgct ggtggttcgg ctcgacggca acaacgtcga ggaaggccgt cgcatcctga ccgaggccaa ccaccccctg gtgacactgg tggcgacgat ggacgaagcc gccgacaagg ccgctgagct ggcgagcgcc taagtgagta agatagg
Expected Protein Sequence
MAHHHHHHMD LFEYQAKELF AKHNVPSTPG RVTDTAEGAK AIATEIGRPV MVKAQVKIGG RGKAGGVKYA ATPQDAYEHA KNILGLDIKG HIVKKLLVAE ASDIAEEYYL SFLLDRANRT YLAMCSVEGG MEIEEVAATK PERLAKVPVN AVKGVDLDFA RSIAEQGHLP AEVLDTAAVT IAKLWELFVA EDATLVEVNP LVRTPDHKIL ALDAKITLDG NADFRQPGHA EFEDRAATDP LELKAKEHDL NYVKLDGQVG IIGNGAGLVM STLDVVAYAG EKHGGVKPAN FLDIGGGASA EVMAAGLDVV LGDQQVKSVF VNVFGGITSC DAVATGIVKA LGMLGDEANK PLVVRLDGNN VEEGRRILTE ANHPLVTLVA TMDEAADKAA ELASA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggatc ttttcgagta tcaagccaag gagttattcg ccaagcacaa cgtgcccagc acgccgggtc gggtgaccga cacagccgag ggtgccaagg ctatcgccac ggagatcggg cgtccggtga tggtcaaagc gcaggtcaag atcggcggcc ggggcaaggc cggtggcgtc aaatacgccg cgaccccaca agacgcgtac gagcacgcca agaacatcct cggcctggac atcaaaggac acatcgtcaa gaaactgctg gtcgctgagg ctagcgatat cgccgaggag tactacctat ccttcctgct cgaccgggcc aaccgcacct acctggcgat gtgctcggtg gagggcggca tggagatcga agaggtagcg gccaccaaac ccgagcggct cgccaaagtc ccggtgaatg ccgtcaaggg cgttgaccta gatttcgcgc ggtccatcgc cgaacagggt catcttccgg ccgaggtgct cgacaccgca gcggtcacca tcgccaagct gtgggagctc ttcgtcgccg aggacgcgac gctggttgag gtcaacccgt tggtgcggac gcctgaccac aagatcctcg cgctggatgc caagatcacc ctcgacggca acgccgattt ccgtcagcct ggccatgccg agttcgagga tcgagctgcc accgatccac tggagttgaa ggccaaggag cacgacctca actacgtcaa gctggacggt caggtgggga tcatcggcaa tggcgcgggc ttggtgatgt cgactctcga cgtcgtcgcg tatgccggtg agaagcacgg cggagtcaag ccggccaact tcctggatat cggcggcggc gcttcggccg aggtgatggc cgcgggtctg gacgtggtgc tgggcgacca gcaggtcaag agcgtgttcg tcaacgtctt cggtggcatc acctcgtgcg atgcggtggc gaccgggatc gtcaaggcgc tgggcatgct gggtgacgaa gccaacaagc cgctggtggt tcggctcgac ggcaacaacg tcgaggaagg ccgtcgcatc ctgaccgagg ccaaccaccc cctggtgaca ctggtggcga cgatggacga agccgccgac aaggccgctg agctggcgag cgcctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for MytuD.00541.a.B1.GE27584
HARVESTED ON: 8/17/2009
SEQUENCED ON: 9/29/2009
EXPECTED MW: 41kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MDLFEYQAKE LFAKHNVPST PGRVTDTAEG AKAIATEIGR PVMVKAQVKI GGRGKAGGVK YAATPQDAYE HAKNILGLDI KGHIVKKLLV AEASDIAEEY YLSFLLDRAN RTYLAMCSVE GGMEIEEVAA TKPERLAKVP VNAVKGVDLD FARSIAEQGH LPAEVLDTAA VTIAKLWELF VAEDATLVEV NPLVRTPDHK ILALDAKITL DGNADFRQPG HAEFEDRAAT DPLELKAKEH DLNYVKLDGQ VGIIGNGAGL VMSTLDVVAY AGEKHGGVKP ANFLDIGGGA SAEVMAAGLD VVLGDQQVKS VFVNVFGGIT SCDAVATGIV KALGMLGDEA NKPLVVRLDG NNVEEGRRIL TEANHPLVTL VATMDEAADK AAELASA
Validated NT Sequence
tcaccaccac caccaccata tggatctttt cgagtatcaa gccaaggagt tattcgccaa gcacaacgtg cccagcacgc cgggtcgggt gaccgacaca gccgagggtg ccaaggctat cgccacggag atcgggcgtc cggtgatggt caaagcgcag gtcaagatcg gcggccgggg caaggccggt ggcgtcaaat acgccgcgac cccacaagac gcgtacgagc acgccaagaa catcctcggc ctggacatca aaggacacat cgtcaagaaa ctgctggtcg ctgaggctag cgatatcgcc gaggagtact acctatcctt cctgctcgac cgggccaacc gcacctacct ggcgatgtgc tcggtggagg gcggcatgga gatcgaagag gtagcggcca ccaaacccga gcggctcgcc aaagtcccgg tgaatgccgt caagggcgtt gacctagatt tcgcgcggtc catcgccgaa cagggtcatc ttccggccga ggtgctcgac accgcagcgg tcaccatcgc caagctgtgg gagctcttcg tcgccgagga cgcgacgctg gttgaggtca acccgttggt gcggacgcct gaccacaaga tcctcgcgct ggatgccaag atcaccctcg acggcaacgc cgatttccgt cagcctggcc atgccgagtt cgaggatcga gctgccaccg atccactgga gttgaaggcc aaggagcacg acctcaacta cgtcaagctg gacggtcagg tggggatcat cggcaatggc gcgggcttgg tgatgtcgac tctcgacgtc gtcgcgtatg ccggtgagaa gcacggcgga gtcaagccgg ccaacttcct ggatatcggc ggcggcgctt cggccgaggt gatggccgcg ggtctggacg tggtgctggg cgaccagcag gtcaagagcg tgttcgtcaa cgtcttcggt ggcatcacct cgtgcgatgc ggtggcgacc gggatcgtca aggcgctggg catgctgggt gacgaagcca acaagccgct ggtggttcgg ctcgacggca acaacgtcga ggaaggccgt cgcatcctga ccgaggccaa ccaccccctg gtgacactgg tggcgacgat ggacgaagcc gccgacaagg ccgctgagct ggcgagcgcc taagtgagta agatagg
Expected Protein Sequence
MAHHHHHHMD LFEYQAKELF AKHNVPSTPG RVTDTAEGAK AIATEIGRPV MVKAQVKIGG RGKAGGVKYA ATPQDAYEHA KNILGLDIKG HIVKKLLVAE ASDIAEEYYL SFLLDRANRT YLAMCSVEGG MEIEEVAATK PERLAKVPVN AVKGVDLDFA RSIAEQGHLP AEVLDTAAVT IAKLWELFVA EDATLVEVNP LVRTPDHKIL ALDAKITLDG NADFRQPGHA EFEDRAATDP LELKAKEHDL NYVKLDGQVG IIGNGAGLVM STLDVVAYAG EKHGGVKPAN FLDIGGGASA EVMAAGLDVV LGDQQVKSVF VNVFGGITSC DAVATGIVKA LGMLGDEANK PLVVRLDGNN VEEGRRILTE ANHPLVTLVA TMDEAADKAA ELASA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggatc ttttcgagta tcaagccaag gagttattcg ccaagcacaa cgtgcccagc acgccgggtc gggtgaccga cacagccgag ggtgccaagg ctatcgccac ggagatcggg cgtccggtga tggtcaaagc gcaggtcaag atcggcggcc ggggcaaggc cggtggcgtc aaatacgccg cgaccccaca agacgcgtac gagcacgcca agaacatcct cggcctggac atcaaaggac acatcgtcaa gaaactgctg gtcgctgagg ctagcgatat cgccgaggag tactacctat ccttcctgct cgaccgggcc aaccgcacct acctggcgat gtgctcggtg gagggcggca tggagatcga agaggtagcg gccaccaaac ccgagcggct cgccaaagtc ccggtgaatg ccgtcaaggg cgttgaccta gatttcgcgc ggtccatcgc cgaacagggt catcttccgg ccgaggtgct cgacaccgca gcggtcacca tcgccaagct gtgggagctc ttcgtcgccg aggacgcgac gctggttgag gtcaacccgt tggtgcggac gcctgaccac aagatcctcg cgctggatgc caagatcacc ctcgacggca acgccgattt ccgtcagcct ggccatgccg agttcgagga tcgagctgcc accgatccac tggagttgaa ggccaaggag cacgacctca actacgtcaa gctggacggt caggtgggga tcatcggcaa tggcgcgggc ttggtgatgt cgactctcga cgtcgtcgcg tatgccggtg agaagcacgg cggagtcaag ccggccaact tcctggatat cggcggcggc gcttcggccg aggtgatggc cgcgggtctg gacgtggtgc tgggcgacca gcaggtcaag agcgtgttcg tcaacgtctt cggtggcatc acctcgtgcg atgcggtggc gaccgggatc gtcaaggcgc tgggcatgct gggtgacgaa gccaacaagc cgctggtggt tcggctcgac ggcaacaacg tcgaggaagg ccgtcgcatc ctgaccgagg ccaaccaccc cctggtgaca ctggtggcga cgatggacga agccgccgac aaggccgctg agctggcgag cgcctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat