MytuD.00317.a
triosephosphate isomerase
| CENTER ID: | MytuD.00317.a |
| ORGANISM: | Mycobacterium tuberculosis H37Rv |
| ASSOCIATED DISEASE: | Tuberculosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MytuD.00317.a.A1.GE25826 | Full length( MytuD.00317.a ) | 1 | 261 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MytuD.00317.a.A1.PW25875 | Full length( MytuD.00317.a ) | 1 | 261 |
Structures
3GVG
DEPOSITED: 3/31/2009
DETERMINATION: XRay
CLONE: MytuD.00317.a.A1.GE25826
PROTEIN: MytuD.00317.a.A1.PW25875
Publications by SSGCID
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_127002 |
| PATRIC ID: | fig|83332.12.peg.1602 |
| RefSeq: | NP_215954.1 |
| TubercuList: | Rv1438 |
| MTB Network Portal: | Rv1438 |
| UniProt: | P9WG43 |
Orthologues for Rv1438
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSRKPLIAGN WKMNLNHYEA IALVQKIAFS LPDKYYDRVD VAVIPPFTDL RSVQTLVDGD KLRLTYGAQD LSPHDSGAYT GDVSGAFLAK LGCSYVVVGH SERRTYHNED DALVAAKAAT ALKHGLTPIV CIGEHLDVRE AGNHVAHNIE QLRGSLAGLL AEQIGSVVIA YEPVWAIGTG RVASAADAQE VCAAIRKELA SLASPRIADT VRVLYGGSVN AKNVGDIVAQ DDVDGGLVGG ASLDGEHFAT LAAIAAGGPL P
NT Sequence
GTGAGCCGCA AGCCGCTGAT AGCCGGCAAC TGGAAGATGA ACCTCAACCA CTACGAGGCG ATCGCGCTGG TGCAAAAGAT CGCGTTCTCG TTGCCGGACA AGTATTACGA CCGGGTTGAC GTCGCGGTGA TCCCGCCGTT TACCGACCTG CGCAGCGTGC AAACCCTGGT CGACGGCGAC AAGCTGCGGT TGACCTATGG TGCACAAGAC TTGTCACCAC ATGACTCCGG TGCCTATACG GGTGACGTCA GCGGCGCCTT TCTGGCCAAG TTGGGGTGCA GTTACGTTGT CGTCGGGCAC TCCGAGCGGC GCACCTATCA CAACGAGGAT GACGCGCTGG TGGCCGCCAA AGCCGCCACC GCACTCAAGC ATGGCTTGAC CCCAATCGTG TGTATTGGCG AGCACCTCGA CGTCCGCGAG GCGGGAAATC ATGTGGCCCA CAACATCGAA CAGTTGCGTG GATCGCTGGC CGGGCTATTG GCCGAGCAGA TCGGCAGCGT CGTCATCGCC TACGAACCGG TCTGGGCGAT CGGCACCGGG CGGGTGGCCA GCGCCGCCGA CGCCCAGGAG GTGTGTGCGG CGATCCGAAA AGAGTTGGCC TCGTTGGCCT CGCCGAGGAT TGCCGATACG GTGCGGGTGC TCTACGGCGG CTCGGTGAAC GCCAAAAACG TCGGCGACAT CGTGGCCCAG GATGACGTCG ATGGTGGCCT GGTCGGCGGG GCGTCGCTGG ACGGGGAGCA TTTCGCGACG CTGGCCGCGA TTGCGGCCGG TGGTCCGTTG CCG
Details for MytuD.00317.a.A1.GE25826
| HARVESTED ON: | 11/19/2008 |
| SEQUENCED ON: | 8/18/2010 |
| EXPECTED MW: | 29kDa |
| OBSERVED MW: | 30kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVSRKPLIA GNWKMNLNHY EAIALVQKIA FSLPDKYYDR VDVAVIPPFT DLRSVQTLVD GDKLRLTYGA QDLSPHDSGA YTGDVSGAFL AKLGCSYVVV GHSERRTYHN EDDALVAAKA ATALKHGLTP IVCIGEHLDV REAGNHVAHN IEQLRGSLAG LLAEQIGSVV IAYEPVWAIG TGRVASAADA QEVCAAIRKE LASLASPRIA DTVRVLYGGS VNAKNVGDIV AQDDVDGGLV GGASLDGEHF ATLAAIAAGG PLP
Validated NT Sequence
ttattacggc aacggaccac cggccgcaat cgcggccagc gtcgcgaaat gctccccgtc cagcgacgcc ccgccgacca ggccaccatc gacgtcatcc tgggccacga tgtcgccgac gtttttggcg ttcaccgagc cgccgtagag cacccgcacc gtatcggcaa tcctcggcga ggccaacgag gccaactctt ttcggatcgc cgcacacacc tcctgggcgt cggcggcgct ggccacccgc ccggtgccga tcgcccagac cggttcgtag gcgatgacga cgctgccgat ctgctcggcc aatagcccgg ccagcgatcc acgcaactgt tcgatgttgt gggccacatg atttcccgcc tcgcggacgt cgaggtgctc gccaatacac acgattgggg tcaagccatg cttgagtgcg gtggcggctt tggcggccac cagcgcgtca tcctcgttgt gataggtgcg ccgctcggag tgcccgacga caacgtaact gcaccccaac ttggccagaa aggcgccgct gacgtcaccc gtataggcac cggagtcatg tggtgacaag tcttgtgcac cataggtcaa ccgcagcttg tcgccgtcga ccagggtttg cacgctgcgc aggtcggtaa acggcgggat caccgcgacg tcaacccggt cgtaatactt gtccggcaac gagaacgcga tcttttgcac cagcgcgatc gcctcgtagt ggttgaggtt catcttccag ttgccggcta tcagcggctt gcggctcacc atcgaaccag gaccctgggt ctgagcttcc agggtaccca tatgatggtg atggtgatga gccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSRKPLIA GNWKMNLNHY EAIALVQKIA FSLPDKYYDR VDVAVIPPFT DLRSVQTLVD GDKLRLTYGA QDLSPHDSGA YTGDVSGAFL AKLGCSYVVV GHSERRTYHN EDDALVAAKA ATALKHGLTP IVCIGEHLDV REAGNHVAHN IEQLRGSLAG LLAEQIGSVV IAYEPVWAIG TGRVASAADA QEVCAAIRKE LASLASPRIA DTVRVLYGGS VNAKNVGDIV AQDDVDGGLV GGASLDGEHF ATLAAIAAGG PLP
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgagccgc aagccgctga tagccggcaa ctggaagatg aacctcaacc actacgaggc gatcgcgctg gtgcaaaaga tcgcgttctc gttgccggac aagtattacg accgggttga cgtcgcggtg atcccgccgt ttaccgacct gcgcagcgtg caaaccctgg tcgacggcga caagctgcgg ttgacctatg gtgcacaaga cttgtcacca catgactccg gtgcctatac gggtgacgtc agcggcgcct ttctggccaa gttggggtgc agttacgttg tcgtcgggca ctccgagcgg cgcacctatc acaacgagga tgacgcgctg gtggccgcca aagccgccac cgcactcaag catggcttga ccccaatcgt gtgtattggc gagcacctcg acgtccgcga ggcgggaaat catgtggccc acaacatcga acagttgcgt ggatcgctgg ccgggctatt ggccgagcag atcggcagcg tcgtcatcgc ctacgaaccg gtctgggcga tcggcaccgg gcgggtggcc agcgccgccg acgcccagga ggtgtgtgcg gcgatccgaa aagagttggc ctcgttggcc tcgccgagga ttgccgatac ggtgcgggtg ctctacggcg gctcggtgaa cgccaaaaac gtcggcgaca tcgtggccca ggatgacgtc gatggtggcc tggtcggcgg ggcgtcgctg gacggggagc atttcgcgac gctggccgcg attgcggccg gtggtccgtt gccgaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for MytuD.00317.a.A1.PW25875
| PURIFICATION DATe: | 1/13/2009 |
| CONCENTRATION: | 30.27mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 20 |
| VIAL VOLUME: | 250µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVSRKPLIA GNWKMNLNHY EAIALVQKIA FSLPDKYYDR VDVAVIPPFT DLRSVQTLVD GDKLRLTYGA QDLSPHDSGA YTGDVSGAFL AKLGCSYVVV GHSERRTYHN EDDALVAAKA ATALKHGLTP IVCIGEHLDV REAGNHVAHN IEQLRGSLAG LLAEQIGSVV IAYEPVWAIG TGRVASAADA QEVCAAIRKE LASLASPRIA DTVRVLYGGS VNAKNVGDIV AQDDVDGGLV GGASLDGEHF ATLAAIAAGG PLP
Validated NT Sequence
ttattacggc aacggaccac cggccgcaat cgcggccagc gtcgcgaaat gctccccgtc cagcgacgcc ccgccgacca ggccaccatc gacgtcatcc tgggccacga tgtcgccgac gtttttggcg ttcaccgagc cgccgtagag cacccgcacc gtatcggcaa tcctcggcga ggccaacgag gccaactctt ttcggatcgc cgcacacacc tcctgggcgt cggcggcgct ggccacccgc ccggtgccga tcgcccagac cggttcgtag gcgatgacga cgctgccgat ctgctcggcc aatagcccgg ccagcgatcc acgcaactgt tcgatgttgt gggccacatg atttcccgcc tcgcggacgt cgaggtgctc gccaatacac acgattgggg tcaagccatg cttgagtgcg gtggcggctt tggcggccac cagcgcgtca tcctcgttgt gataggtgcg ccgctcggag tgcccgacga caacgtaact gcaccccaac ttggccagaa aggcgccgct gacgtcaccc gtataggcac cggagtcatg tggtgacaag tcttgtgcac cataggtcaa ccgcagcttg tcgccgtcga ccagggtttg cacgctgcgc aggtcggtaa acggcgggat caccgcgacg tcaacccggt cgtaatactt gtccggcaac gagaacgcga tcttttgcac cagcgcgatc gcctcgtagt ggttgaggtt catcttccag ttgccggcta tcagcggctt gcggctcacc atcgaaccag gaccctgggt ctgagcttcc agggtaccca tatgatggtg atggtgatga gccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSRKPLIA GNWKMNLNHY EAIALVQKIA FSLPDKYYDR VDVAVIPPFT DLRSVQTLVD GDKLRLTYGA QDLSPHDSGA YTGDVSGAFL AKLGCSYVVV GHSERRTYHN EDDALVAAKA ATALKHGLTP IVCIGEHLDV REAGNHVAHN IEQLRGSLAG LLAEQIGSVV IAYEPVWAIG TGRVASAADA QEVCAAIRKE LASLASPRIA DTVRVLYGGS VNAKNVGDIV AQDDVDGGLV GGASLDGEHF ATLAAIAAGG PLP
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGAGCCGC AAGCCGCTGA TAGCCGGCAA CTGGAAGATG AACCTCAACC ACTACGAGGC GATCGCGCTG GTGCAAAAGA TCGCGTTCTC GTTGCCGGAC AAGTATTACG ACCGGGTTGA CGTCGCGGTG ATCCCGCCGT TTACCGACCT GCGCAGCGTG CAAACCCTGG TCGACGGCGA CAAGCTGCGG TTGACCTATG GTGCACAAGA CTTGTCACCA CATGACTCCG GTGCCTATAC GGGTGACGTC AGCGGCGCCT TTCTGGCCAA GTTGGGGTGC AGTTACGTTG TCGTCGGGCA CTCCGAGCGG CGCACCTATC ACAACGAGGA TGACGCGCTG GTGGCCGCCA AAGCCGCCAC CGCACTCAAG CATGGCTTGA CCCCAATCGT GTGTATTGGC GAGCACCTCG ACGTCCGCGA GGCGGGAAAT CATGTGGCCC ACAACATCGA ACAGTTGCGT GGATCGCTGG CCGGGCTATT GGCCGAGCAG ATCGGCAGCG TCGTCATCGC CTACGAACCG GTCTGGGCGA TCGGCACCGG GCGGGTGGCC AGCGCCGCCG ACGCCCAGGA GGTGTGTGCG GCGATCCGAA AAGAGTTGGC CTCGTTGGCC TCGCCGAGGA TTGCCGATAC GGTGCGGGTG CTCTACGGCG GCTCGGTGAA CGCCAAAAAC GTCGGCGACA TCGTGGCCCA GGATGACGTC GATGGTGGCC TGGTCGGCGG GGCGTCGCTG GACGGGGAGC ATTTCGCGAC GCTGGCCGCG ATTGCGGCCG GTGGTCCGTT GCCGAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA