MytuD.00262.a

GTPase Era

CENTER ID: MytuD.00262.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.00262.a.A1.GE32212 full length 1 300
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.2643
RefSeq: NP_336913.1
TubercuList: Rv2364c
MTB Network Portal: Rv2364c
UniProt: P9WNK9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTEFHSGFVC LVGRPNTGKS TLTNALVGAK VAITSTRPQT TRHAIRGIVH SDDFQIILVD TPGLHRPRTL LGKRLNDLVR ETYAAVDVIG LCIPADEAIG PGDRWIVEQL RSTGPANTTL VVIVTKIDKV PKEKVVAQLV AVSELVTNAA EIVPVSAMTG DRVDLLIDVL AAALPAGPAY YPDGELTDEP EEVLMAELIR EAALQGVRDE LPHSLAVVID EVSPREGRDD LIDVHAALYV ERDSQKGIVI GKGGARLREV GTAARSQIEN LLGTKVYLDL RVKVAKNWQR DPKQLGRLGF
NT Sequence
atgaccgaat tccattctgg ctttgtgtgt ttggtcggcc ggccgaacac cggcaagtcc acgctgacca atgcgctggt cggggcgaaa gtggcaatca cgtcgacgcg cccgcagacc accaggcacg ccattcgtgg gatcgtgcat agcgacgact tccagatcat cctcgtcgac actcccggtc tgcaccggcc gcgcactctg ctgggcaagc ggctcaacga cttggtccgc gaaacctacg cggcggtcga cgtcatcggg ctatgcatcc ccgccgacga ggcgatcggt ccgggagacc ggtggattgt cgagcagctt cgttcgaccg gccctgccaa tacgacactg gtggtcatcg tcaccaagat tgacaaggtg ccgaaagaaa aagtggtcgc ccagctggta gcggtcagcg aactcgtcac gaatgcggcc gagattgtcc cggtttcggc gatgaccggc gaccgggtag acctgttgat cgacgtactg gccgcggcat tgccggccgg ccccgcgtat taccccgacg gtgagttgac tgacgaaccc gaggaggttc tgatggccga gctcatccgc gaggcggccc ttcagggcgt gcgcgatgag ctgccccatt cgctggcggt ggtgattgac gaggtcagtc cacgtgaggg gcgtgacgac ctgatcgacg tacacgccgc gctgtacgtc gagcgagata gtcagaaggg catcgtcatc ggcaagggtg gtgcccggct gcgggaggtg ggtaccgcgg cccgtagcca gatcgaaaac ctgctgggca ccaaggtcta tctcgacttg cgggtcaagg tcgccaagaa ctggcagcgc gaccccaaac agcttggccg actggggttt tag
Details for MytuD.00262.a.A1.GE32212
HARVESTED ON: 4/26/2011
SEQUENCED ON: 5/2/2011
EXPECTED MW: 34kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAQTQG PGSMTEFHSG FVCLVGRPNT GKSTLTNALV GAKVAITSTR PQTTRHAIRG IVHSDDFQII LVDTPGLHRP RTLLGKRLND LVRETYAAVD VIGLCIPADE AIGPGDRWIV EQLRSTGPAN TTLVVIVTKI DKVPKEKVVA QLVAVSELVT NAAEIVPVSA MTGDRVDLLI DVLAAALPAG PAYYPDGELT DEPEEVLMAE LIREAALQGV RDELPHSLAV VIDEVSPREG RDDLIDVHAA LYVERDSQKG IVIGKGGARL REVGTAARSQ IENLLGTKVY LDLRVKVAKN WQRDPKQLGR LGF
Validated NT Sequence
ttattaaaac cccagtcggc caagctgttt ggggtcgcgc tgccagttct tggcgacctt gacccgcaag tcgagataga ccttggtgcc cagcaggttt tcgatctggc tacgggccgc ggtacccacc tcccgcagcc gggcaccacc cttgccgatg acgatgccct tctgactatc tcgctcgacg tacagcgcgg cgtgtacgtc gatcaggtcg tcacgcccct cacgtggact gacctcgtca atcaccaccg ccagcgaatg gggcagctca tcgcgcacgc cctgaagggc cgcctcgcgg atgagctcgg ccatcagaac ctcctcgggt tcgtcagtca actcaccgtc ggggtaatac gcggggccgg ccggcaatgc cgcggccagt acgtcgatca acaggtctac ccggtcgccg gtcatcgccg aaaccgggac aatctcggcc gcattcgtga cgagttcgct gaccgctacc agctgggcga ccactttttc tttcggcacc ttgtcaatct tggtgacgat gaccaccagt gtcgtattgg cagggccggt cgaacgaagc tgctcgacaa tccaccggtc tcccggaccg atcgcctcgt cggcggggat gcatagcccg atgacgtcga ccgccgcgta ggtttcgcgg accaagtcgt tgagccgctt gcccagcaga gtgcgcggcc ggtgcagacc gggagtgtcg acgaggatga tctggaagtc gtcgctatgc acgatcccac gaatggcgtg cctggtggtc tgcgggcgcg tcgacgtgat tgccactttc gccccgacca gcgcattggt cagcgtggac ttgccggtgt tcggccggcc gaccaaacac acaaagccag aatggaattc ggtcatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccang nna
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTEFHSGFV CLVGRPNTGK STLTNALVGA KVAITSTRPQ TTRHAIRGIV HSDDFQIILV DTPGLHRPRT LLGKRLNDLV RETYAAVDVI GLCIPADEAI GPGDRWIVEQ LRSTGPANTT LVVIVTKIDK VPKEKVVAQL VAVSELVTNA AEIVPVSAMT GDRVDLLIDV LAAALPAGPA YYPDGELTDE PEEVLMAELI REAALQGVRD ELPHSLAVVI DEVSPREGRD DLIDVHAALY VERDSQKGIV IGKGGARLRE VGTAARSQIE NLLGTKVYLD LRVKVAKNWQ RDPKQLGRLG F
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaccgaattc cattctggct ttgtgtgttt ggtcggccgg ccgaacaccg gcaagtccac gctgaccaat gcgctggtcg gggcgaaagt ggcaatcacg tcgacgcgcc cgcagaccac caggcacgcc attcgtggga tcgtgcatag cgacgacttc cagatcatcc tcgtcgacac tcccggtctg caccggccgc gcactctgct gggcaagcgg ctcaacgact tggtccgcga aacctacgcg gcggtcgacg tcatcgggct atgcatcccc gccgacgagg cgatcggtcc gggagaccgg tggattgtcg agcagcttcg ttcgaccggc cctgccaata cgacactggt ggtcatcgtc accaagattg acaaggtgcc gaaagaaaaa gtggtcgccc agctggtagc ggtcagcgaa ctcgtcacga atgcggccga gattgtcccg gtttcggcga tgaccggcga ccgggtagac ctgttgatcg acgtactggc cgcggcattg ccggccggcc ccgcgtatta ccccgacggt gagttgactg acgaacccga ggaggttctg atggccgagc tcatccgcga ggcggccctt cagggcgtgc gcgatgagct gccccattcg ctggcggtgg tgattgacga ggtcagtcca cgtgaggggc gtgacgacct gatcgacgta cacgccgcgc tgtacgtcga gcgagatagt cagaagggca tcgtcatcgg caagggtggt gcccggctgc gggaggtggg taccgcggcc cgtagccaga tcgaaaacct gctgggcacc aaggtctatc tcgacttgcg ggtcaaggtc gccaagaact ggcagcgcga ccccaaacag cttggccgac tggggtttta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a