MytuD.00163.a

phenylalanyl-tRNA synthetase subunit alpha

CENTER ID:	MytuD.00163.a
ORGANISM:	Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE:	Tuberculosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MytuD.00163.a.A1.GE27574	Full length( MytuD.00163.a )		1	341

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_127036
PATRIC ID:	fig\|83332.12.peg.1836
RefSeq:	NP_216165.1
TubercuList:	Rv1649
MTB Network Portal:	Rv1649
UniProt:	P9WFU3

Orthologues for Rv1649

Species	% Identity	% Coverage	Clones	Proteins
avium	87	100	0	0
marinum	87	100	1	0
paratuberculosis	87	100	0	0
ulcerans	87	100	1	0
leprae	85	100	0	0
smegmatis	83	100	1	0
abscessus	80	99	1	1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLSPEALTTA VDAAQQAIAL ADTLDVLARV KTEHLGDRSP LALARQALAV LPKEQRAEAG KRVNAARNAA QRSYDERLAT LRAERDAAVL VAEGIDVTLP STRVPAGARH PIIMLAEHVA DTFIAMGWEL AEGPEVETEQ FNFDALNFPA DHPARGEQDT FYIAPEDSRQ LLRTHTSPVQ IRTLLARELP VYIISIGRTF RTDELDATHT PIFHQVEGLA VDRGLSMAHL RGTLDAFARA EFGPSARTRI RPHFFPFTEP SAEVDVWFAN KIGGAAWVEW GGCGMVHPNV LRATGIDPDL YSGFAFGMGL ERTLQFRNGI PDMRDMVEGD VRFSLPFGVG A

NT Sequence

ATGTTGTCGC CGGAGGCATT GACCACGGCG GTCGACGCCG CCCAGCAGGC CATCGCCCTA GCGGACACCC TGGACGTCCT GGCGCGCGTC AAGACGGAGC ATCTCGGCGA CCGCTCGCCG TTGGCGCTGG CGCGGCAGGC GCTGGCCGTG CTGCCCAAAG AACAGCGAGC CGAGGCCGGT AAGCGCGTCA ACGCCGCCCG CAATGCCGCT CAGCGCAGCT ACGACGAACG GCTGGCGACG CTGCGTGCCG AGCGCGACGC GGCCGTGCTG GTGGCCGAAG GTATCGATGT CACATTGCCC TCGACTCGGG TGCCGGCCGG CGCCCGGCAC CCGATCATCA TGTTGGCCGA ACACGTCGCC GACACGTTCA TCGCGATGGG ATGGGAACTG GCCGAGGGGC CCGAGGTGGA GACCGAGCAG TTCAACTTCG ACGCCCTCAA CTTCCCTGCC GACCACCCTG CGCGCGGCGA ACAAGATACC TTCTACATCG CGCCGGAGGA TTCGCGGCAG CTGCTGCGCA CCCATACCTC ACCGGTGCAG ATTCGCACCC TGCTAGCGCG TGAGCTGCCG GTCTACATCA TCTCGATCGG TCGTACCTTT CGCACCGACG AACTCGACGC CACCCACACG CCCATCTTCC ATCAGGTGGA AGGCCTAGCG GTGGACCGCG GTCTGTCGAT GGCTCACCTA CGTGGAACGC TGGACGCTTT TGCGCGCGCC GAGTTCGGGC CGTCTGCGCG GACCCGGATC CGGCCACACT TCTTCCCCTT CACCGAACCG TCCGCCGAGG TCGATGTGTG GTTTGCCAAC AAGATTGGCG GCGCCGCCTG GGTGGAGTGG GGCGGGTGCG GAATGGTGCA TCCGAACGTG TTGCGGGCCA CCGGCATTGA TCCCGATCTC TACTCCGGTT TCGCGTTCGG GATGGGGTTG GAACGCACCC TGCAGTTTCG CAACGGCATT CCTGACATGC GCGACATGGT CGAAGGCGAC GTCCGATTCT CGTTGCCGTT CGGGGTGGGT GCC

Details for MytuD.00163.a.A1.GE27574

HARVESTED ON:	7/21/2009
SEQUENCED ON:	7/28/2009
EXPECTED MW:	39kDa
OBSERVED MW:	39kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	84
PERCENT COVERAGE:	75

Validated AA Sequence

MPLSAATTNG WRRCVPSATR PCWWPKVSMS HCPRLGCRPA PGTRSSCWPX HVADTFIAMG WELAEGSEVE TEQFNFDALN FPADHPARGE QDTFYIAPED SRQLLRTHTS PVQIRTLLAR ELPVYIISIG RTFRTDELDA THTPIFHQVE GLAVDRGLSM AHLRGTLDAF ARAEFGPSAR TRIRPHFFPF TEPSAEVDVW FANKIGGAAW VEWGGCGMVH PNVLRATGID PDLYSGFAFG MGLERTLQFR NGIPDMRDMV EGDVRFSLPF GVGA

Validated NT Sequence

ggctcatcac cntcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgttgtcn ccggaggcat tgaccacggc ggtcgacgcc gcccagcagg ccatcgccct agcggacacc ctggacgtcc tggcgcgcgt caagacggag catctcggcg accgctcgcc gttggcgctg gcgcggcagg cgctggccgt gctgcccaaa gaacagcgag ccgaggccgg taagcgcgtc aacgccgccc gcaatgccgc tcagcgcagc tacgacgaac ggctggcgac gctgcgtgcc gagcgcgacg cggccgtgct ggtggccgaa ggtatcgatg tcacattgcc ctcgactcgg gtgccggccg gcgcccggca cccgatcatc atgttggccc naacacgtcg ccgacacgtt catcgcgatg ggatgggaac tggccgaggg gtccgaggtg gagaccgagc agttcaactt cgacgccctc aacttccctg ccgaccaccc tgcgcgcggc gaacaagata ccttctacat cgcgccggag gattcgcggc agctgctgcg cacccatacc tcaccggtgc agattcgcac cctgctagcg cgtgagctgc cggtctacat catctcgatc ggtcgtacct ttcgcaccga cgaactcgac gccacccaca cgcccatctt ccatcaggtg gaaggcctag cggtggaccg cggtctgtcg atggctcacc tacgtggaac gctggacgct tttgcgcgcg ccgagttcgg gccgtctgcg cggacccgga tccggccaca cttcttcccc ttcaccgaac cgtccgccga ggtcgatgtg tggtttgcca acaagattgg cggcgccgcc tgggtggagt ggggcgggtg cggaatggtg catccgaacg tgttgcgggc caccggcatt gatcccgatc tctactccgg tttcgcgttc gggatggggt tggaacgcac cctgcagttt cgcaacggca ttcctgacat gcgcgacatg gtcgaaggcg acgtccgatt ctcgttgccg ttcggggtgg gtgccta

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMLSPEALTT AVDAAQQAIA LADTLDVLAR VKTEHLGDRS PLALARQALA VLPKEQRAEA GKRVNAARNA AQRSYDERLA TLRAERDAAV LVAEGIDVTL PSTRVPAGAR HPIIMLAEHV ADTFIAMGWE LAEGPEVETE QFNFDALNFP ADHPARGEQD TFYIAPEDSR QLLRTHTSPV QIRTLLAREL PVYIISIGRT FRTDELDATH TPIFHQVEGL AVDRGLSMAH LRGTLDAFAR AEFGPSARTR IRPHFFPFTE PSAEVDVWFA NKIGGAAWVE WGGCGMVHPN VLRATGIDPD LYSGFAFGMG LERTLQFRNG IPDMRDMVEG DVRFSLPFGV GA

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttgtcgccg gaggcattga ccacggcggt cgacgccgcc cagcaggcca tcgccctagc ggacaccctg gacgtcctgg cgcgcgtcaa gacggagcat ctcggcgacc gctcgccgtt ggcgctggcg cggcaggcgc tggccgtgct gcccaaagaa cagcgagccg aggccggtaa gcgcgtcaac gccgcccgca atgccgctca gcgcagctac gacgaacggc tggcgacgct gcgtgccgag cgcgacgcgg ccgtgctggt ggccgaaggt atcgatgtca cattgccctc gactcgggtg ccggccggcg cccggcaccc gatcatcatg ttggccgaac acgtcgccga cacgttcatc gcgatgggat gggaactggc cgaggggccc gaggtggaga ccgagcagtt caacttcgac gccctcaact tccctgccga ccaccctgcg cgcggcgaac aagatacctt ctacatcgcg ccggaggatt cgcggcagct gctgcgcacc catacctcac cggtgcagat tcgcaccctg ctagcgcgtg agctgccggt ctacatcatc tcgatcggtc gtacctttcg caccgacgaa ctcgacgcca cccacacgcc catcttccat caggtggaag gcctagcggt ggaccgcggt ctgtcgatgg ctcacctacg tggaacgctg gacgcttttg cgcgcgccga gttcgggccg tctgcgcgga cccggatccg gccacacttc ttccccttca ccgaaccgtc cgccgaggtc gatgtgtggt ttgccaacaa gattggcggc gccgcctggg tggagtgggg cgggtgcgga atggtgcatc cgaacgtgtt gcgggccacc ggcattgatc ccgatctcta ctccggtttc gcgttcggga tggggttgga acgcaccctg cagtttcgca acggcattcc tgacatgcgc gacatggtcg aaggcgacgt ccgattctcg ttgccgttcg gggtgggtgc caaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa