MytuD.00149.b

S-adenosylmethionine-dependent methyltransferase UmaA (SAM-dependent methyltransferase UmaA) (EC 2.1.1.-) (Rv0469)

CENTER ID: MytuD.00149.b
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: in PDB
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:
IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
MytuD.00149.b.B1.GE43826 full length 1 286
MytuD.00149.b.HX1.GE43827 full length 1 286
MytuD.00149.b.MB1.GE43828 full length 1 286
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
MytuD.00149.b.B1.PW39156 full length 1 286

Structures

7L9U
DEPOSITED: 1/5/2021
DETERMINATION: XRay
CLONE: MytuD.00149.b.B1.GE43826
PROTEIN: MytuD.00149.b.B1.PW38903
7LXI
DEPOSITED: 3/3/2021
DETERMINATION: XRay
CLONE: MytuD.00149.b.B1.GE43826
PROTEIN: MytuD.00149.b.B1.PW38903
7MCJ
DEPOSITED: 4/2/2021
DETERMINATION: XRay
CLONE: MytuD.00149.b.B1.GE43826
PROTEIN: MytuD.00149.b.B1.PW38903
8T1A
DEPOSITED: 6/2/2023
DETERMINATION: XRay
CLONE: MytuD.00149.b.B1.GE43826
PROTEIN: MytuD.00149.b.B1.PW39156

External Resources

RESOURCE REFERENCE ID
PATRIC ID: fig|83332.111.peg.513
TubercuList: Rv0469
MTB Network Portal: Rv0469
UniProt: Q6MX39

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTELRPFYEE SQSIYDVSDE FFSLFLDPTM AYTCAYFERE DMTLEEAQNA KFDLALDKLH LEPGMTLLDI GCGWGGGLQR AIENYDVNVI GITLSRNQFE YSKAKLAKIP TERSVQVRLQ GWDEFTDKVD RIVSIGAFEA FKMERYAAFF ERSYDILPDD GRMLLHTILT YTQKQMHEMG VKVTMSDVRF MKFIGEEIFP GGQLPAQEDI FKFAQAADFS VEKVQLLQQH YARTLNIWAA NLEANKDRAI ALQSEEIYNK YMHYLTGCEH FFRKGISNVG QFTLTK
NT Sequence
atgactgagt taaggccctt ttacgaagag tcgcaatcga tttacgacgt ttccgacgag tttttctcac tgtttctaga ccccacgatg gcttacacct gcgcgtactt cgagcgtgag gacatgactc tcgaagaagc gcaaaacgcg aagttcgatt tggcgctgga caagttgcat cttgagcccg ggatgacgct gctcgatatt ggctgcggct ggggtggtgg gctgcaacga gcgatcgaga actacgatgt gaacgtcatc ggtatcacgc tcagtcgcaa tcagttcgag tacagcaaag cgaaattggc gaaaattccc accgaacgca gcgtccaggt gcggctgcag ggctgggatg agttcacgga caaggtcgac cgtattgtca gcatcggtgc cttcgaagca ttcaaaatgg agcgttatgc ggcattcttt gagcgttcct acgacatact tccagatgac ggccggatgc tgctgcacac aattctgacc tatacgcaga agcagatgca tgagatgggc gtcaaggtga cgatgagcga tgtgcggttt atgaaattca tcggcgaaga aatttttccg ggcggacagt taccggcgca ggaagacatc ttcaaatttg cgcaggcggc ggacttttcg gtggagaagg tgcaattgct gcagcagcat tacgctcgga cgctaaacat ctgggcggcg aatctggagg ctaacaagga ccgcgccatt gctcttcagt ccgaggagat ttacaacaaa tacatgcact atctgaccgg atgtgagcac ttcttccgca agggcatcag caacgtggga cagttcacac tgaccaagta g
Details for MytuD.00149.b.B1.GE43826
HARVESTED ON: 8/24/2020
SEQUENCED ON: 8/28/2020
EXPECTED MW: 34kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMT ELRPFYEESQ SIYDVSDEFF SLFLDPTMAY TCAYFEREDM TLEEAQNAKF DLALDKLHLE PGMTLLDIGC GWGGGLQRAI ENYDVNVIGI TLSRNQFEYS KAKLAKIPTE RSVQVRLQGW DEFTDKVDRI VSIGAFEAFK MERYAAFFER SYDILPDDGR MLLHTILTYT QKQMHEMGVK VTMSDVRFMK FIGEEIFPGG QLPAQEDIFK FAQAADFSVE KVQLLQQHYA RTLNIWAANL EANKDRAIAL QSEEIYNKYM HYLTGCEHFF RKGISNXXQF TLTK
Validated NT Sequence
atggctcacc accaccacca ccatatgact gagttaaggc ccttttacga agagtcgcaa tcgatttacg acgtttccga cgagtttttc tcactgtttc tagaccccac gatggcttac acctgcgcgt acttcgagcg tgaggacatg actctcgaag aagcgcaaaa cgcgaagttc gatttggcgc tggacaagtt gcatcttgag cccgggatga cgctgctcga tattggctgc ggctggggtg gtgggctgca acgagcgatc gagaactacg atgtgaacgt catcggtatc acgctcagtc gcaatcagtt cgagtacagc aaagcgaaat tggcgaaaat tcccaccgaa cgcagcgtcc aggtgcggct gcagggctgg gatgagttca cggacaaggt cgaccgtatt gtcagcatcg gtgccttcga agcattcaaa atggagcgtt atgcggcatt ctttgagcgt tcctacgaca tacttccaga tgacggccgg atgctgctgc acacaattct gacctatacg cagaagcaga tgcatgagat gggcgtcaag gtgacgatga gcgatgtgcg gtttatgaaa ttcatcggcg aagaaatttt tccgggcgga cagttaccgg cgcaggaaga catcttcaaa tttgcgcagg cggcggactt ttcggtggag aaggtgcaat tgctgcagca gcattacgct cggacgctaa acatctgggc ggcgaatctg gaggctaaca aggaccgcgc cattgctctt cagtccgagg agatttacaa caaatacatg cactatctga ccggatgtga gcacttcttc cgcaagggca tcagcaacnn nngncagttc acactgacca agtaagtgag taagat
Expected Protein Sequence
MAHHHHHHMT ELRPFYEESQ SIYDVSDEFF SLFLDPTMAY TCAYFEREDM TLEEAQNAKF DLALDKLHLE PGMTLLDIGC GWGGGLQRAI ENYDVNVIGI TLSRNQFEYS KAKLAKIPTE RSVQVRLQGW DEFTDKVDRI VSIGAFEAFK MERYAAFFER SYDILPDDGR MLLHTILTYT QKQMHEMGVK VTMSDVRFMK FIGEEIFPGG QLPAQEDIFK FAQAADFSVE KVQLLQQHYA RTLNIWAANL EANKDRAIAL QSEEIYNKYM HYLTGCEHFF RKGISNVGQF TLTK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgactg agttaaggcc cttttacgaa gagtcgcaat cgatttacga cgtttccgac gagtttttct cactgtttct agaccccacg atggcttaca cctgcgcgta cttcgagcgt gaggacatga ctctcgaaga agcgcaaaac gcgaagttcg atttggcgct ggacaagttg catcttgagc ccgggatgac gctgctcgat attggctgcg gctggggtgg tgggctgcaa cgagcgatcg agaactacga tgtgaacgtc atcggtatca cgctcagtcg caatcagttc gagtacagca aagcgaaatt ggcgaaaatt cccaccgaac gcagcgtcca ggtgcggctg cagggctggg atgagttcac ggacaaggtc gaccgtattg tcagcatcgg tgccttcgaa gcattcaaaa tggagcgtta tgcggcattc tttgagcgtt cctacgacat acttccagat gacggccgga tgctgctgca cacaattctg acctatacgc agaagcagat gcatgagatg ggcgtcaagg tgacgatgag cgatgtgcgg tttatgaaat tcatcggcga agaaattttt ccgggcggac agttaccggc gcaggaagac atcttcaaat ttgcgcaggc ggcggacttt tcggtggaga aggtgcaatt gctgcagcag cattacgctc ggacgctaaa catctgggcg gcgaatctgg aggctaacaa ggaccgcgcc attgctcttc agtccgagga gatttacaac aaatacatgc actatctgac cggatgtgag cacttcttcc gcaagggcat cagcaacgtg ggacagttca cactgaccaa gtgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
Details for MytuD.00149.b.HX1.GE43827
HARVESTED ON: 8/24/2020
SEQUENCED ON: 8/28/2020
EXPECTED MW: 90kDa
OBSERVED MW: 90kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 30
Validated AA Sequence
MTLEEAQNAK FDLALDKLHL EPGMTLLDIG CGWGGGLQRA IENYDVNVIG ITLSRNQFEY SKAKLAKIPT ERSVQVRLQG WDEFTDKVDR IVSIGAFEAF KMERYAAFFE RSYDILPDDG RMLLHTILTY TQKQMHEMGV KVTMSDVRFM KFIGEEIFPG GQLPAQEDIF KFAQAADFSV EKVQLLQQHY ARTLNIWAAN LEANKDRAIA LQSEEIYNKY MHYLTGCEHF FRKGISNVGQ FTLTK
Validated NT Sequence
cnnnnnnnng gnctttnnna gcagccggat cctcgagaag cttggctgca gaacttgttt gtgctgttta ttacttggtc agtgtgaact gtcccacgtt gctgatgccc ttgcggaaga agtgctcaca tccggtcaga tagtgcatgt atttgttgta aatctcctcg gactgaagag caatggcgcg gtccttgtta gcctccagat tcgccgccca gatgtttagc gtccgagcgt aatgctgctg cagcaattgc accttctcca ccgaaaagtc cgccgcctgc gcaaatttga agatgtcttc ctgcgccggt aactgtccgc ccggaaaaat ttcttcgccg atgaatttca taaaccgcac atcgctcatc gtcaccttga cgcccatctc atgcatctgc ttctgcgtat aggtcagaat tgtgtgcagc agcatccggc cgtcatctgg aagtatgtcg taggaacgct caaagaatgc cgcataacgc tccattttga atgcttcgaa ggcaccgatg ctgacaatac ggtcgacctt gtccgtgaac tcatcccagc cctgcagccg cacctggacg ctgcgttcgg tgggaatttt cgccaatttc gctttgctgt actcgaactg attgcgactg agcgtgatac cgatgacgtt cacatcgtag ttctcgatcg ctcgttgcag cccaccaccc cagccgcagc caatatcgag cagcgtcatc ccgggctcaa gatgcaactt gtccagcgcc aaatcgaact tcgcgttttg cgcttcttcn agagtcatgt cctcacgctc gaagtacgcg cagtgtaagc catcgngggg nnctagaaac agtgagaaaa aactcgtcgg aaacgtcgta aatcgattgc nactnnttcg taaanggnct taactcagtc atcgaaccag naccctgggt ctgagcttcc agggtacccg cttcnncanc naaccagcan ngttacgggn agnnnnncan ngctcngctt tttcgtcggn caacccttcg antntnngcc agatcatcna tgcctnnncg
Expected Protein Sequence
MAHHHHHHMK NKEILAVVEA VSNEKALPRE KIFEALESAL ATATKKKYEQ EIDVRVQIDR KSGDFDTFRR WLVVDEVTQP TKEITLEAAR YEDESLNLGD YVEDQIESVT FDRITTQTAK QVIVQKVREA ERAMVVDQFR EHEGEIITGV VKKVNRDNIS LDLGNNAEAV ILREDMLPRE NFRPGDRVRG VLYSVRPEAR GAQLFVTRSK PEMLIELFRI EVPEIGEEVI EIKAAARDPG SRAKIAVKTN DKRIDPVGAC VGMRGARVQA VSTELGGERI DIVLWDDNPA QFVINAMAPA DVASIVVDED KHTMDIAVEA GNLAQAIGRN GQNVRLASQL SGWELNVMTV DDLQAKHQAE AHAAIDTFTK YLDIDEDFAT VLVEEGFSTL EELAYVPMKE LLEIEGLDEP TVEALRERAK NALATIAQAQ EESLGDNKPA DDLLNLEGVD RDLAFKLAAR GVCTLEDLAE QGIDDLADIE GLTDEKAGAL IMAARNICWF GDEAGTLEAQ TQGPGSMTEL RPFYEESQSI YDVSDEFFSL FLDPTMAYTC AYFEREDMTL EEAQNAKFDL ALDKLHLEPG MTLLDIGCGW GGGLQRAIEN YDVNVIGITL SRNQFEYSKA KLAKIPTERS VQVRLQGWDE FTDKVDRIVS IGAFEAFKME RYAAFFERSY DILPDDGRML LHTILTYTQK QMHEMGVKVT MSDVRFMKFI GEEIFPGGQL PAQEDIFKFA QAADFSVEKV QLLQQHYART LNIWAANLEA NKDRAIALQS EEIYNKYMHY LTGCEHFFRK GISNVGQFTL TK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgaagaacaa agaaattttg gctgtagttg aagccgtatc caatgaaaag gcgctacctc gcgagaagat tttcgaagca ttggaaagcg cgctggcgac agcaacaaag aaaaaatatg aacaagagat cgacgtccgc gtacagatcg atcgcaaaag cggtgatttt gacactttcc gtcgctggtt agttgttgat gaagtcaccc agccgaccaa ggaaatcacc cttgaagccg cacgttatga agatgaaagc ctgaacctgg gcgattacgt tgaagatcag attgagtctg ttacctttga ccgtatcact acccagacgg caaaacaggt tatcgtgcag aaagtgcgtg aagccgaacg tgcgatggtg gttgatcagt tccgtgaaca cgaaggtgaa atcatcaccg gcgtggtgaa aaaagtaaac cgcgacaaca tctctctgga tctgggcaac aacgctgaag ccgtgatcct gcgcgaagat atgctgccgc gtgaaaactt ccgccctggc gaccgcgttc gtggcgtgct ctattccgtt cgcccggaag cgcgtggcgc gcaactgttc gtcactcgtt ccaagccgga aatgctgatc gaactgttcc gtattgaagt gccagaaatc ggcgaagaag tgattgaaat taaagcagcg gctcgcgatc cgggttctcg tgcgaaaatc gcggtgaaaa ccaacgataa acgtatcgat ccggtaggtg cttgcgtagg tatgcgtggc gcgcgtgttc aggcggtgtc tactgaactg ggtggcgagc gtatcgatat cgtcctgtgg gatgataacc cggcgcagtt cgtgattaac gcaatggcac cggcagacgt tgcttctatc gtggtggatg aagataaaca caccatggat atcgccgttg aagccggtaa cctggcgcag gcgattggcc gtaacggtca gaacgtgcgt ctggcttcgc agctgagcgg ttgggaactc aacgtgatga ccgttgacga cctgcaggct aagcatcagg cggaagcgca cgcagcgatc gacaccttca ccaaatatct cgacatcgac gaagacttcg cgactgttct ggtagaagaa ggcttctcga cgctggaaga attggcctat gtgccgatga aagagctgtt ggaaatcgaa ggccttgatg agccgaccgt tgaagcactg cgcgagcgtg ctaaaaatgc actggccacc attgcacagg cccaggaaga aagcctcggt gataacaaac cggctgacga tctgctgaac cttgaagggg tagatcgtga tttggcattc aaactggccg cccgtggcgt ttgtacgctg gaagatctcg ccgaacaggg cattgatgat ctggctgata tcgaagggtt gaccgacgaa aaagccggag cactgattat ggctgcccgt aacatttgct ggttcggtga cgaagcgggt accctggaag ctcagaccca gggtcctggt tcgatgactg agttaaggcc cttttacgaa gagtcgcaat cgatttacga cgtttccgac gagtttttct cactgtttct agaccccacg atggcttaca cctgcgcgta cttcgagcgt gaggacatga ctctcgaaga agcgcaaaac gcgaagttcg atttggcgct ggacaagttg catcttgagc ccgggatgac gctgctcgat attggctgcg gctggggtgg tgggctgcaa cgagcgatcg agaactacga tgtgaacgtc atcggtatca cgctcagtcg caatcagttc gagtacagca aagcgaaatt ggcgaaaatt cccaccgaac gcagcgtcca ggtgcggctg cagggctggg atgagttcac ggacaaggtc gaccgtattg tcagcatcgg tgccttcgaa gcattcaaaa tggagcgtta tgcggcattc tttgagcgtt cctacgacat acttccagat gacggccgga tgctgctgca cacaattctg acctatacgc agaagcagat gcatgagatg ggcgtcaagg tgacgatgag cgatgtgcgg tttatgaaat tcatcggcga agaaattttt ccgggcggac agttaccggc gcaggaagac atcttcaaat ttgcgcaggc ggcggacttt tcggtggaga aggtgcaatt gctgcagcag cattacgctc ggacgctaaa catctgggcg gcgaatctgg aggctaacaa ggaccgcgcc attgctcttc agtccgagga gatttacaac aaatacatgc actatctgac cggatgtgag cacttcttcc gcaagggcat cagcaacgtg ggacagttca cactgaccaa gaaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for MytuD.00149.b.MB1.GE43828
HARVESTED ON: data unavailable
SEQUENCED ON: 8/28/2020
EXPECTED MW: 78kDa
OBSERVED MW: 50kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 97
PERCENT COVERAGE: 20
Validated AA Sequence
MERYAAFFER SYDILPXDGR MLLHTILTYT QKQMHEMGVK VTMSDVRFMK FIGEEIFPGG QLPAQEDIFK FAQAADFSVE KVQLLQQHYA RTLNIWAXNL EANKDRAIAL QSEEIXNKYM HYLTGCEHFF RKGISNVGQF TLTK
Validated NT Sequence
tnnnntttcn nnnnnnnngn nnncnnccgg atcctcgaag aagcttggct gcagaacttg ttcgtgctgt tnattacttg gtcagtgtga actgtcccac gttgctgatg cccttgcgga agaagtgctc acatccggtc agatagtgca tgtatttgtt gnaaatctcc tcggactgaa gagcaatggc gcggtccttg ttagcctcca gattcnccgc ccagatgttt agcgtccgag cgtaatgctg ctgcagcaat tgcaccttct ccaccgaaaa gtccgccgcc tgcgcaaatt tgaagatgtc ttcctgcgcc ggtaactgtc cgcccggaaa aatttcttcg ccgatgaatt tcataaaccg cacatcgctc atcgtcacct tgacncccat ctcatgcatc tgcttctgcg tataggtcag aattgtgtgc agcagcatcc ggccgtcntc tggaagtatg tcgtangaac gctcaaagaa tgccgcataa cgctccattt tgaatgcttc naangcaccg atgctgacaa tacngtcgac cttgtccgtg aactcatccc agccctgcan ccgcacctgg acnctgcgtt cggtgggaat tttcgccaat ttcnctttng ctgtactcna actgattgcg actgancgtg ataccgatga cgttcacatc gtantctcga tcgctcgttg cagccc
Expected Protein Sequence
MAHHHHHHMK IEEGKLVIWI NGDKGYNGLA EVGKKFEKDT GIKVTVEHPD KLEEKFPQVA ATGDGPDIIF WAHDRFGGYA QSGLLAEITP DKAFQDKLYP FTWDAVRYNG KLIAYPIAVE ALSLIYNKDL LPNPPKTWEE IPALDKELKA KGKSALMFNL QEPYFTWPLI AADGGYAFKY ENGKYDIKDV GVDNAGAKAG LTFLVDLIKN KHMNADTDYS IAEAAFNKGE TAMTINGPWA WSNIDTSKVN YGVTVLPTFK GQPSKPFVGV LSAGINAASP NKELAKEFLE NYLLTDEGLE AVNKDKPLGA VALKSYEEEL AKDPRIAATM ENAQKGEIMP NIPQMSAFWY AVRTAVINAA SGRQTVDEAL KDAQTNSSSN NNNNNNNNNL GIEGRISEGT LEAQTQGPGS MTELRPFYEE SQSIYDVSDE FFSLFLDPTM AYTCAYFERE DMTLEEAQNA KFDLALDKLH LEPGMTLLDI GCGWGGGLQR AIENYDVNVI GITLSRNQFE YSKAKLAKIP TERSVQVRLQ GWDEFTDKVD RIVSIGAFEA FKMERYAAFF ERSYDILPDD GRMLLHTILT YTQKQMHEMG VKVTMSDVRF MKFIGEEIFP GGQLPAQEDI FKFAQAADFS VEKVQLLQQH YARTLNIWAA NLEANKDRAI ALQSEEIYNK YMHYLTGCEH FFRKGISNVG QFTLTK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcaca tgaaaatcga agaaggtaaa ctggtaatct ggattaacgg cgataaaggc tataacggtc tcgctgaagt cggtaagaaa ttcgagaaag ataccggaat taaagtcacc gttgagcatc cggataaact ggaagagaaa ttcccacagg ttgcggcaac tggcgatggc cctgacatta tcttctgggc acacgaccgc tttggtggct acgctcaatc tggcctgttg gctgaaatca ccccggacaa agcgttccag gacaagctgt atccgtttac ctgggatgcc gtacgttaca acggcaagct gattgcttac ccgatcgctg ttgaagcgtt atcgctgatt tataacaaag atctgctgcc gaacccgcca aaaacctggg aagagatccc ggcgctggat aaagaactga aagcgaaagg taagagcgcg ctgatgttca acctgcaaga accgtacttc acctggccgc tgattgctgc tgacgggggt tatgcgttca agtatgaaaa cggcaagtac gacattaaag acgtgggcgt ggataacgct ggcgcgaaag cgggtctgac cttcctggtt gacctgatta aaaacaaaca catgaatgca gacaccgatt actccatcgc agaagctgcc tttaataaag gcgaaacagc gatgaccatc aacggcccgt gggcatggtc caacatcgac accagcaaag tgaattatgg tgtaacggta ctgccgacct tcaagggtca accatccaaa ccgttcgttg gcgtgctgag cgcaggtatt aacgccgcca gtccgaacaa agagctggca aaagagttcc tcgaaaacta tctgctgact gatgaaggtc tggaagcggt taataaagac aaaccgctgg gtgccgtagc gctgaagtct tacgaggaag agttggcgaa agatccacgt attgccgcca ctatggaaaa cgcccagaaa ggtgaaatca tgccgaacat cccgcagatg tccgctttct ggtatgccgt gcgtactgcg gtgatcaacg ccgccagcgg tcgtcagact gtcgatgaag ccctgaaaga cgcgcagact aattcgagct cgaacaacaa caacaacaac aataacaaca acctcgggat cgagggaagg atttcagaag gtaccctgga agctcagacc cagggtcctg gttcgatgac tgagttaagg cccttttacg aagagtcgca atcgatttac gacgtttccg acgagttttt ctcactgttt ctagacccca cgatggctta cacctgcgcg tacttcgagc gtgaggacat gactctcgaa gaagcgcaaa acgcgaagtt cgatttggcg ctggacaagt tgcatcttga gcccgggatg acgctgctcg atattggctg cggctggggt ggtgggctgc aacgagcgat cgagaactac gatgtgaacg tcatcggtat cacgctcagt cgcaatcagt tcgagtacag caaagcgaaa ttggcgaaaa ttcccaccga acgcagcgtc caggtgcggc tgcagggctg ggatgagttc acggacaagg tcgaccgtat tgtcagcatc ggtgccttcg aagcattcaa aatggagcgt tatgcggcat tctttgagcg ttcctacgac atacttccag atgacggccg gatgctgctg cacacaattc tgacctatac gcagaagcag atgcatgaga tgggcgtcaa ggtgacgatg agcgatgtgc ggtttatgaa attcatcggc gaagaaattt ttccgggcgg acagttaccg gcgcaggaag acatcttcaa atttgcgcag gcggcggact tttcggtgga gaaggtgcaa ttgctgcagc agcattacgc tcggacgcta aacatctggg cggcgaatct ggaggctaac aaggaccgcg ccattgctct tcagtccgag gagatttaca acaaatacat gcactatctg accggatgtg agcacttctt ccgcaagggc atcagcaacg tgggacagtt cacactgacc aagtaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for MytuD.00149.b.B1.PW39156
PURIFICATION DATe: 10/19/2022
CONCENTRATION: 26.15mg/ml
OBSERVED MW: 33kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 8
VIAL VOLUME: 110µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMT ELRPFYEESQ SIYDVSDEFF SLFLDPTMAY TCAYFEREDM TLEEAQNAKF DLALDKLHLE PGMTLLDIGC GWGGGLQRAI ENYDVNVIGI TLSRNQFEYS KAKLAKIPTE RSVQVRLQGW DEFTDKVDRI VSIGAFEAFK MERYAAFFER SYDILPDDGR MLLHTILTYT QKQMHEMGVK VTMSDVRFMK FIGEEIFPGG QLPAQEDIFK FAQAADFSVE KVQLLQQHYA RTLNIWAANL EANKDRAIAL QSEEIYNKYM HYLTGCEHFF RKGISNXXQF TLTK
Validated NT Sequence
atggctcacc accaccacca ccatatgact gagttaaggc ccttttacga agagtcgcaa tcgatttacg acgtttccga cgagtttttc tcactgtttc tagaccccac gatggcttac acctgcgcgt acttcgagcg tgaggacatg actctcgaag aagcgcaaaa cgcgaagttc gatttggcgc tggacaagtt gcatcttgag cccgggatga cgctgctcga tattggctgc ggctggggtg gtgggctgca acgagcgatc gagaactacg atgtgaacgt catcggtatc acgctcagtc gcaatcagtt cgagtacagc aaagcgaaat tggcgaaaat tcccaccgaa cgcagcgtcc aggtgcggct gcagggctgg gatgagttca cggacaaggt cgaccgtatt gtcagcatcg gtgccttcga agcattcaaa atggagcgtt atgcggcatt ctttgagcgt tcctacgaca tacttccaga tgacggccgg atgctgctgc acacaattct gacctatacg cagaagcaga tgcatgagat gggcgtcaag gtgacgatga gcgatgtgcg gtttatgaaa ttcatcggcg aagaaatttt tccgggcgga cagttaccgg cgcaggaaga catcttcaaa tttgcgcagg cggcggactt ttcggtggag aaggtgcaat tgctgcagca gcattacgct cggacgctaa acatctgggc ggcgaatctg gaggctaaca aggaccgcgc cattgctctt cagtccgagg agatttacaa caaatacatg cactatctga ccggatgtga gcacttcttc cgcaagggca tcagcaacnn nngncagttc acactgacca agtaagtgag taagat
Expressed Protein Sequence
MAHHHHHHMT ELRPFYEESQ SIYDVSDEFF SLFLDPTMAY TCAYFEREDM TLEEAQNAKF DLALDKLHLE PGMTLLDIGC GWGGGLQRAI ENYDVNVIGI TLSRNQFEYS KAKLAKIPTE RSVQVRLQGW DEFTDKVDRI VSIGAFEAFK MERYAAFFER SYDILPDDGR MLLHTILTYT QKQMHEMGVK VTMSDVRFMK FIGEEIFPGG QLPAQEDIFK FAQAADFSVE KVQLLQQHYA RTLNIWAANL EANKDRAIAL QSEEIYNKYM HYLTGCEHFF RKGISNVGQF TLTK
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACTG AGTTAAGGCC CTTTTACGAA GAGTCGCAAT CGATTTACGA CGTTTCCGAC GAGTTTTTCT CACTGTTTCT AGACCCCACG ATGGCTTACA CCTGCGCGTA CTTCGAGCGT GAGGACATGA CTCTCGAAGA AGCGCAAAAC GCGAAGTTCG ATTTGGCGCT GGACAAGTTG CATCTTGAGC CCGGGATGAC GCTGCTCGAT ATTGGCTGCG GCTGGGGTGG TGGGCTGCAA CGAGCGATCG AGAACTACGA TGTGAACGTC ATCGGTATCA CGCTCAGTCG CAATCAGTTC GAGTACAGCA AAGCGAAATT GGCGAAAATT CCCACCGAAC GCAGCGTCCA GGTGCGGCTG CAGGGCTGGG ATGAGTTCAC GGACAAGGTC GACCGTATTG TCAGCATCGG TGCCTTCGAA GCATTCAAAA TGGAGCGTTA TGCGGCATTC TTTGAGCGTT CCTACGACAT ACTTCCAGAT GACGGCCGGA TGCTGCTGCA CACAATTCTG ACCTATACGC AGAAGCAGAT GCATGAGATG GGCGTCAAGG TGACGATGAG CGATGTGCGG TTTATGAAAT TCATCGGCGA AGAAATTTTT CCGGGCGGAC AGTTACCGGC GCAGGAAGAC ATCTTCAAAT TTGCGCAGGC GGCGGACTTT TCGGTGGAGA AGGTGCAATT GCTGCAGCAG CATTACGCTC GGACGCTAAA CATCTGGGCG GCGAATCTGG AGGCTAACAA GGACCGCGCC ATTGCTCTTC AGTCCGAGGA GATTTACAAC AAATACATGC ACTATCTGAC CGGATGTGAG CACTTCTTCC GCAAGGGCAT CAGCAACGTG GGACAGTTCA CACTGACCAA GTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT