MytuD.00149.b
S-adenosylmethionine-dependent methyltransferase UmaA (SAM-dependent methyltransferase UmaA) (EC 2.1.1.-) (Rv0469)
CENTER ID:
MytuD.00149.b
ORGANISM:
Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE:
Tuberculosis
CURRENT STATUS:
in PDB
COMMUNITY REQUEST:
True
NIH RISK GROUP:
2
SELECT AGENT:
False
NIH PRIORITY
pathogens category:
IIIC
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO
AA
START
AA
STOP
ORDER
MATERIAL
MytuD.00149.b.B1.GE43826
full length
info
1
286
MytuD.00149.b.B1.GE43826
MytuD.00149.b.HX1.GE43827
full length
info
1
286
MytuD.00149.b.HX1.GE43827
MytuD.00149.b.MB1.GE43828
full length
info
1
286
MytuD.00149.b.MB1.GE43828
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis . Get sequence information
using the button in the "info" column.
Proteins
CENTER REFERENCE ID
DOMAIN/REGION DESCRIPTION
INFO
AA START
AA STOP
ORDER MATERIAL
MytuD.00149.b.B1.PW39156
full length
info
1
286
PW39156
Structures
7L9U
DEPOSITED: 1/5/2021
DETERMINATION: XRay
CLONE: MytuD.00149.b.B1.GE43826
PROTEIN: MytuD.00149.b.B1.PW38903
7LXI
DEPOSITED: 3/3/2021
DETERMINATION: XRay
CLONE: MytuD.00149.b.B1.GE43826
PROTEIN: MytuD.00149.b.B1.PW38903
7MCJ
DEPOSITED: 4/2/2021
DETERMINATION: XRay
CLONE: MytuD.00149.b.B1.GE43826
PROTEIN: MytuD.00149.b.B1.PW38903
8T1A
DEPOSITED: 6/2/2023
DETERMINATION: XRay
CLONE: MytuD.00149.b.B1.GE43826
PROTEIN: MytuD.00149.b.B1.PW39156
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTELRPFYEE SQSIYDVSDE FFSLFLDPTM AYTCAYFERE DMTLEEAQNA KFDLALDKLH LEPGMTLLDI GCGWGGGLQR AIENYDVNVI GITLSRNQFE YSKAKLAKIP TERSVQVRLQ GWDEFTDKVD RIVSIGAFEA FKMERYAAFF ERSYDILPDD GRMLLHTILT YTQKQMHEMG VKVTMSDVRF MKFIGEEIFP GGQLPAQEDI FKFAQAADFS VEKVQLLQQH YARTLNIWAA NLEANKDRAI ALQSEEIYNK YMHYLTGCEH FFRKGISNVG QFTLTK
NT Sequence
atgactgagt taaggccctt ttacgaagag tcgcaatcga tttacgacgt ttccgacgag tttttctcac tgtttctaga ccccacgatg gcttacacct gcgcgtactt cgagcgtgag gacatgactc tcgaagaagc gcaaaacgcg aagttcgatt tggcgctgga caagttgcat cttgagcccg ggatgacgct gctcgatatt ggctgcggct ggggtggtgg gctgcaacga gcgatcgaga actacgatgt gaacgtcatc ggtatcacgc tcagtcgcaa tcagttcgag tacagcaaag cgaaattggc gaaaattccc accgaacgca gcgtccaggt gcggctgcag ggctgggatg agttcacgga caaggtcgac cgtattgtca gcatcggtgc cttcgaagca ttcaaaatgg agcgttatgc ggcattcttt gagcgttcct acgacatact tccagatgac ggccggatgc tgctgcacac aattctgacc tatacgcaga agcagatgca tgagatgggc gtcaaggtga cgatgagcga tgtgcggttt atgaaattca tcggcgaaga aatttttccg ggcggacagt taccggcgca ggaagacatc ttcaaatttg cgcaggcggc ggacttttcg gtggagaagg tgcaattgct gcagcagcat tacgctcgga cgctaaacat ctgggcggcg aatctggagg ctaacaagga ccgcgccatt gctcttcagt ccgaggagat ttacaacaaa tacatgcact atctgaccgg atgtgagcac ttcttccgca agggcatcag caacgtggga cagttcacac tgaccaagta g
Details for MytuD.00149.b.B1.GE43826
HARVESTED ON:
8/24/2020
SEQUENCED ON:
8/28/2020
EXPECTED MW:
34kDa
OBSERVED MW:
34kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Good (10-50)
TOTAL EXPRESSION LEVEL:
Moderate Expression
SOLUBLE EXPRESSION LEVEL
Low Expression
EXPRESSION HOST:
BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:
99
PERCENT COVERAGE:
100
Details for MytuD.00149.b.HX1.GE43827
HARVESTED ON:
8/24/2020
SEQUENCED ON:
8/28/2020
EXPECTED MW:
90kDa
OBSERVED MW:
90kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Good (10-50)
TOTAL EXPRESSION LEVEL:
Moderate Expression
SOLUBLE EXPRESSION LEVEL
Low Expression
EXPRESSION HOST:
BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:
100
PERCENT COVERAGE:
30
Details for MytuD.00149.b.MB1.GE43828
HARVESTED ON:
data unavailable
SEQUENCED ON:
8/28/2020
EXPECTED MW:
78kDa
OBSERVED MW:
50kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Good (10-50)
TOTAL EXPRESSION LEVEL:
Low Expression
SOLUBLE EXPRESSION LEVEL
Low Expression
EXPRESSION HOST:
BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:
97
PERCENT COVERAGE:
20
Details for MytuD.00149.b.B1.PW39156
PURIFICATION DATe:
10/19/2022
CONCENTRATION:
26.15mg/ml
OBSERVED MW:
33kDa
EXPRESSION LEVEL:
Low Expression
PROTEIN PURIFICATION BUFFER:
25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST:
BL 21 (DE3) Rosetta
VIAL COUNT (approx.):
8
VIAL VOLUME:
110µl
PERCENT IDENTITY:
99
PERCENT COVERAGE:
100