MytuD.00148.d

PROBABLE INTEGRASE

CENTER ID: MytuD.00148.d
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.00148.d.A1.GE30707 Full length( MytuD.00148.d ) 1 332
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.2957
RefSeq: NP_217162.1
TubercuList: Rv2646
MTB Network Portal: Rv2646
UniProt: I6XEU5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNTATRVRLA RKRADRLNLK LIKNGHHFRL RDADEITLAV GHLGVVEAFL AAAKSQNKPP GPPPSLHAPP SWRRDIDDYL LNLNAAGQRP ATIRLRKTVL CAAAHGLGRP PADVTAEHLL DWLGKQQHLS PEGRKTYRST LRGFFVWAYE MDRVRDYVAD SLPKVRCPKQ PPRPAGDDVW QAALAKADRR IELMIRLAGE AGLRRAEAAQ AHTGDLMDGG LLLVHGKGGK RRIVPISDYL AALIRDTPHG YLFPNGTGGH LTAEHVGKLV SRALPGDATM HTLRHRYATR AYRGSHNLRA VQQLLGHASI VTTERYTALC DDEVRAAAAA AW
NT Sequence
GTGAACACCG CGACCCGGGT CCGGCTGGCC CGCAAACGCG CCGACCGGCT CAATCTGAAA CTAATCAAGA ACGGCCACCA CTTCAGGTTG CGTGACGCCG ACGAGATCAC GCTGGCGGTC GGGCACCTAG GGGTGGTGGA AGCCTTCCTG GCGGCGGCCA AGTCGCAAAA CAAGCCGCCC GGTCCGCCGC CGAGCCTCCA CGCCCCGCCA TCCTGGCGGC GCGACATCGA CGACTACCTG CTCAACCTGA ACGCCGCCGG TCAACGCCCA GCGACGATCC GGCTACGCAA GACGGTGCTG TGCGCAGCCG CCCACGGCCT CGGCCGCCCA CCCGCCGACG TCACCGCCGA ACACCTCCTG GACTGGCTAG GCAAACAGCA GCACCTCTCC CCAGAGGGCC GCAAAACCTA TCGCAGCACG TTGCGGGGCT TCTTCGTGTG GGCCTACGAA ATGGACCGGG TGCGCGACTA TGTCGCAGAC TCCCTGCCTA AGGTGCGCTG CCCGAAACAG CCGCCCCGCC CGGCCGGCGA CGACGTCTGG CAAGCGGCGC TGGCCAAGGC CGACCGTCGA ATCGAGCTGA TGATCCGCCT AGCCGGTGAG GCCGGGCTGC GACGCGCCGA AGCCGCCCAG GCGCACACCG GCGACTTGAT GGACGGCGGG CTTCTCCTCG TTCACGGCAA AGGTGGTAAA CGCCGTATTG TGCCGATCAG CGACTACTTG GCCGCGCTCA TCCGCGACAC CCCGCACGGC TACCTGTTCC CCAACGGCAC CGGCGGCCAC CTCACCGCCG AACACGTGGG AAAACTCGTC TCCCGGGCAT TACCCGGTGA CGCGACCATG CACACCCTGC GGCACCGATA CGCCACCCGC GCCTACCGCG GCTCCCACAA CTTGCGAGCT GTACAACAAC TTCTCGGTCA CGCCTCGATC GTGACAACAG AACGCTACAC AGCGCTGTGC GACGACGAGG TGCGCGCCGC AGCAGCAGCC GCATGG
Details for MytuD.00148.d.A1.GE30707
HARVESTED ON: 11/9/2010
SEQUENCED ON: 11/15/2010
EXPECTED MW: 38kDa
OBSERVED MW: 38kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 51
Validated AA Sequence
MDRVRDYVAD SLPKVRCPKQ PPRPAGDDVW QAALAKADRR IELMIRLAGE AGLRRAEAAQ AHTGDLMDGG LLLVHGKGGK RRIVPISDYL AALIRDTPHG YLFPNGTGGH LTAEHVGKLV SRALPGDATM HTLRHRYATR AYRGSHNLRA VQQLLGHASI VTTERYTALC DDEVRAAAAA AW
Validated NT Sequence
ttattaccat gcggctgctg ctgcggcgcg cacctcgtcg tcgcacagcg ctgtgtagcg ttctgttgtc acgatcgagg cgtgaccgag aagttgttgt acagctcgca agttgtggga gccgcggtag gcgcgggtgg cgtatcggtg ccgcagggtg tgcatggtcg cgtcaccggg taatgcccgg gagacgagtt ttcccacgtg ttcggcggtg aggtggccgc cggtgccgtt ggggaacagg tagccgtgcg gggtgtcgcg gatgagcgcg gccaagtagt cgctgatcgg cacaatacgg cgtttaccac ctttgccgtg aacgaggaga agcccgccgt ccatcaagtc gccggtgtgc gcctgggcgg cttcggcgcg tcgcagcccg gcctcaccgg ctaggcggat catcagctcg attcgacggt cggccttggc cagcgccgct tgccagacgt cgtcgccggc cgggcggggc ggctgtttcg ggcagcgcac cttaggcagg gagtctgcga catagtcgcg cacccggtcc atttcgtagg cccacacgaa gaagccccgc aacgtgctgc gataggtttt gcggccctct gggggagagg tgctgctgtt tgcctagcca gtccaggagg tgttcggcgg tgacgtcggc gggtgggcgg ccgaggccgt gggcggctgc gcacagcacc gtcttgcgta gccggatcgt cgctgggcgt tgaccggcgg cgttcaggtt gagcaggtag tcgtcgatgt cgcgccgcca ggatggcggg gcgtggaggc tcggcggcgg accgggcggc ttgttttgcg acttggccgc cgccaggaag gcttccacca cccctaggtg cccgaccgcc agcgtgatct cgtcggcgtc acgcaacctg aagtggtggc cgttcttgat tagtttcaga ttgagccggt cggcgcgttt gcgggccagc cggacccggg tcgcggtgtt caccatcgaa ccaggaccct gggtctgagc ttccagg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVNTATRVR LARKRADRLN LKLIKNGHHF RLRDADEITL AVGHLGVVEA FLAAAKSQNK PPGPPPSLHA PPSWRRDIDD YLLNLNAAGQ RPATIRLRKT VLCAAAHGLG RPPADVTAEH LLDWLGKQQH LSPEGRKTYR STLRGFFVWA YEMDRVRDYV ADSLPKVRCP KQPPRPAGDD VWQAALAKAD RRIELMIRLA GEAGLRRAEA AQAHTGDLMD GGLLLVHGKG GKRRIVPISD YLAALIRDTP HGYLFPNGTG GHLTAEHVGK LVSRALPGDA TMHTLRHRYA TRAYRGSHNL RAVQQLLGHA SIVTTERYTA LCDDEVRAAA AAAW
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgaacacc gcgacccggg tccggctggc ccgcaaacgc gccgaccggc tcaatctgaa actaatcaag aacggccacc acttcaggtt gcgtgacgcc gacgagatca cgctggcggt cgggcaccta ggggtggtgg aagccttcct ggcggcggcc aagtcgcaaa acaagccgcc cggtccgccg ccgagcctcc acgccccgcc atcctggcgg cgcgacatcg acgactacct gctcaacctg aacgccgccg gtcaacgccc agcgacgatc cggctacgca agacggtgct gtgcgcagcc gcccacggcc tcggccgccc acccgccgac gtcaccgccg aacacctcct ggactggcta ggcaaacagc agcacctctc cccagagggc cgcaaaacct atcgcagcac gttgcggggc ttcttcgtgt gggcctacga aatggaccgg gtgcgcgact atgtcgcaga ctccctgcct aaggtgcgct gcccgaaaca gccgccccgc ccggccggcg acgacgtctg gcaagcggcg ctggccaagg ccgaccgtcg aatcgagctg atgatccgcc tagccggtga ggccgggctg cgacgcgccg aagccgccca ggcgcacacc ggcgacttga tggacggcgg gcttctcctc gttcacggca aaggtggtaa acgccgtatt gtgccgatca gcgactactt ggccgcgctc atccgcgaca ccccgcacgg ctacctgttc cccaacggca ccggcggcca cctcaccgcc gaacacgtgg gaaaactcgt ctcccgggca ttacccggtg acgcgaccat gcacaccctg cggcaccgat acgccacccg cgcctaccgc ggctcccaca acttgcgagc tgtacaacaa cttctcggtc acgcctcgat cgtgacaaca gaacgctaca cagcgctgtg cgacgacgag gtgcgcgccg cagcagcagc cgcatggtaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa