SSGCID: Target Page

MytuD.00114.a

Uncharacterized protein Rv0525

CENTER ID:	MytuD.00114.a
ORGANISM:	Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE:	Tuberculosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MytuD.00114.a.B1.GE37905	full length		1	202

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|83332.12.peg.582
RefSeq:	NP_215039.1
RefSeq:	YP_006513858.1
TubercuList:	Rv0525
MTB Network Portal:	Rv0525
UniProt:	O06391

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPEETQVHVV RHGEVHNPTG ILYGRLPGFH LSATGAAQAA AVADALADRD IVAVIASPLQ RAQETAAPIA ARHDLAVETD PDLIESANFF EGRRVGPGDG AWRDPRVWWQ LRNPFTPSWG EPYVDIAARM TTAVDKARVR GAGHEVVCVS HQLPVWTLRL YLTGKRLWHD PRRRDCALAS VTSLIYDGDR LVDVVYSQPA AL

NT Sequence

atgcccgagg aaacccaagt ccacgtggtg cgccacggtg aggtgcacaa ccctaccggc atcctgtacg ggcggctgcc cggattccac ctgtccgcaa ccggcgcggc gcaggccgcc gccgtcgccg acgcgctggc cgaccgcgac atcgtcgcgg taatcgcatc gcccttgcag cgtgcccagg agaccgccgc gcccatcgcc gcccggcatg accttgcggt ggagacagac ccggatctga tcgaatcggc caacttcttc gagggccgcc gcgtcggccc cggtgacggg gcatggcgcg acccgcgggt gtggtggcag ctgcgtaacc cgttcacccc gtcgtggggt gagccttacg tggatatcgc tgcccgaatg acgaccgcgg tggacaaggc acgtgtccgc ggcgccggcc atgaggtggt gtgcgtcagc catcagctgc cggtgtggac gctgcggctg tatctgaccg gtaagcgcct ctggcacgat ccgcgccgtc gggactgcgc actggcctcg gtgacgtcgt tgatctacga cggcgaccgc ctggttgacg tggtgtattc gcagccggcg gcgctttga

Details for MytuD.00114.a.B1.GE37905

HARVESTED ON:	4/10/2014
SEQUENCED ON:	4/8/2014
EXPECTED MW:	23kDa
OBSERVED MW:	23kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMP EETQVHVVRH GEVHNPTGIL YGRLPGFHLS ATGAAQAAAV ADALADRDIV AVIASPLQRA QETAAPIAAR HDLAVETDPD LIESANFFEG RRVGPGDGAW RDPRVWWQLR NPFTPSWGEP YVDIAARMTT AVDKARVRGA GHEVVCVSHQ LPVWTLRLYL TGKRLWHDPR RRDCALASVT SLIYDGDRLV DVVYSQPAAL

Validated NT Sequence

cnctagnata ttttgtttaa ctttaagaag gagatatacc atggctcacc accaccacca ccatatgccc gaggaaaccc aagtccacgt ggtgcgccac ggtgaggtgc acaaccctac cggcatcctg tacgggcggc tgcccggatt ccacctgtcc gcaaccggcg cggcgcaggc cgccgccgtc gccgacgcgc tggccgaccg cgacatcgtc gcggtaatcg catcgccctt gcagcgtgcc caggagaccg ccgcgcccat cgccgcccgg catgaccttg cggtggagac agacccggat ctgatcgaat cggccaactt cttcgagggc cgccgcgtcg gccccggtga cggggcatgg cgcgacccgc gggtgtggtg gcagctgcgt aacccgttca ccccgtcgtg gggtgagcct tacgtggata tcgctgcccg aatgacgacc gcggtggaca aggcacgtgt ccgcggcgcc ggccatgagg tggtgtgcgt cagccatcag ctgccggtgt ggacgctgcg gctgtatctg accggtaagc gcctctggca cgatccgcgc cgtcgggact gcgcactggc ctcggtgacg tcgttgatct acgacggcga ccgcctggtt gacgtggtgt attcgcagcc ggcggcgctt taagtgagta agataggatc cggctgctaa cancccccca aaanga

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcccg aggaaaccca agtccacgtg gtgcgccacg gtgaggtgca caaccctacc ggcatcctgt acgggcggct gcccggattc cacctgtccg caaccggcgc ggcgcaggcc gccgccgtcg ccgacgcgct ggccgaccgc gacatcgtcg cggtaatcgc atcgcccttg cagcgtgccc aggagaccgc cgcgcccatc gccgcccggc atgaccttgc ggtggagaca gacccggatc tgatcgaatc ggccaacttc ttcgagggcc gccgcgtcgg ccccggtgac ggggcatggc gcgacccgcg ggtgtggtgg cagctgcgta acccgttcac cccgtcgtgg ggtgagcctt acgtggatat cgctgcccga atgacgaccg cggtggacaa ggcacgtgtc cgcggcgccg gccatgaggt ggtgtgcgtc agccatcagc tgccggtgtg gacgctgcgg ctgtatctga ccggtaagcg cctctggcac gatccgcgcc gtcgggactg cgcactggcc tcggtgacgt cgttgatcta cgacggcgac cgcctggttg acgtggtgta ttcgcagccg gcggcgcttt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat