MytuD.00111.a

Probable glutamine synthetase GlnA4 (Glutamine synthase) (GS-II) (Rv2860c)

CENTER ID: MytuD.00111.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:
IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
MytuD.00111.a.B1.GE43820 full length 1 457
MytuD.00111.a.HX1.GE43821 full length 1 457
MytuD.00111.a.MB1.GE43822 full length 1 457
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.111.peg.3181
TubercuList: Rv2860c
MTB Network Portal: Rv2860c
UniProt: I6X5K1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTGPGSPPLA WTELERLVAA GDVDTVIVAF TDMQGRLAGK RISGRHFVDD IATRGVECCS YLLAVDVDLN TVPGYAMASW DTGYGDMVMT PDLSTLRLIP WLPGTALVIA DLVWADGSEV AVSPRSILRR QLDRLKARGL VADVATELEF IVFDQPYRQA WASGYRGLTP ASDYNIDYAI LASSRMEPLL RDIRLGMAGA GLRFEAVKGE CNMGQQEIGF RYDEALVTCD NHAIYKNGAK EIADQHGKSL TFMAKYDERE GNSCHIHVSL RGTDGSAVFA DSNGPHGMSS MFRSFVAGQL ATLREFTLCY APTINSYKRF ADSSFAPTAL AWGLDNRTCA LRVVGHGQNI RVECRVPGGD VNQYLAVAAL IAGGLYGIER GLQLPEPCVG NAYQGADVER LPVTLADAAV LFEDSALVRE AFGEDVVAHY LNNARVELAA FNAAVTDWER IRGFERL
NT Sequence
gtgaccggcc ccggttcgcc gccgttggcg tggaccgagt tggagcgact ggtcgcggcc ggtgacgtcg acaccgtcat cgtcgcgttc accgacatgc agggccggct ggccggcaaa cggatatcgg gccggcattt cgtcgacgac atagccaccc gcggcgtcga gtgctgcagt tatctgctgg ccgtggacgt cgacctgaac acggtgcccg gctatgcgat ggccagttgg gacaccggct acggcgatat ggtgatgacg ccggacttgt ccactctgcg gctgattcct tggctaccgg gaacggcgct ggtgatcgcc gacctggtct gggccgacgg cagcgaggtc gccgtctcgc cgcgcagcat tctgcgccgt cagctcgatc ggctcaaggc gcgcggactg gtcgccgatg tggccaccga gctggagttc atcgtgttcg accagccgta tcgccaggca tgggccagcg ggtatcgcgg gctgaccccg gccagcgact acaacatcga ctacgcgata ttggcatcct cgcggatgga gccgttgctg cgcgacatcc ggttgggtat ggccggtgcg ggtctgcgat tcgaggcggt caaaggcgaa tgcaacatgg gccagcagga gatcgggttt cgttacgacg aggcgctggt cacctgcgac aaccatgcga tctacaagaa cggcgccaag gaaatcgccg accagcacgg caagagccta acgttcatgg cgaaatacga tgaacgcgaa ggtaatagct gtcacatcca tgtctcgctg cgtggcacgg atggctccgc ggtgtttgcc gacagtaacg ggccgcacgg catgtcgtcg atgttccgca gcttcgtcgc cggccagttg gccacgttgc gcgaattcac gctgtgctat gcgccgacca ttaactccta caagcgattt gccgatagca gtttcgcgcc gacggcgctg gcttgggggc tggacaatcg cacctgcgcc ctgcgggtgg ttggccacgg gcaaaacatc cgggtcgaat gccgggttcc cggcggtgat gtcaaccagt acctggcggt ggcggctctc attgctggag ggttgtacgg tatcgagcgg ggccttcagc tgcccgagcc ctgtgtcggc aacgcctacc aaggcgccga tgtcgaacgg ctgccggtta cgctggccga cgccgcggtg ctgttcgagg attctgcgct ggtgcgcgag gcgttcggcg aggatgttgt cgcgcactac ctgaacaacg cgcgtgtgga gctggcggcg ttcaacgcgg cggtcaccga ttgggagagg atacgtggat ttgagcgcct ctag
Details for MytuD.00111.a.B1.GE43820
HARVESTED ON: 8/24/2020
SEQUENCED ON: 8/28/2020
EXPECTED MW: 51kDa
OBSERVED MW: 51kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 92
PERCENT COVERAGE: 57
Validated AA Sequence
MAHHHHHHMV TGPGSPPLAW TELERLVAAG DVDTVIVAFT DMQGRLAGKR ISGRHFVDDI ATRGVECCSY LLAVDVDLNT VPGYAMASWD TGYGDMVMTP DLSTLRLIPW LPGTALVIAD LVWADGSEVA VSPRSILRRQ LDRLKARGLV ADVATELEFI VFDQPYRQAW ASGYRGLTPA SDYNIDYAIL ASSRMEPLLR DIRLGMAGAG LRFEAVKGEC NMGQQEIGFR YDEALVTCDN PCDLQERRQG NRRPARQEPN VHGEIR
Validated NT Sequence
atggctcacc accaccacca ccatatggtg accggccccg gttcgccgcc gttggcgtgg accgagttgg agcgactggt cgcggccggt gacgtcgaca ccgtcatcgt cgcgttcacc gacatgcagg gccggctggc cggcaaacgg atatcgggcc ggcatttcgt cgacgacata gccacccgcg gcgtcgagtg ctgcagttat ctgctggccg tggacgtcga cctgaacacg gtgcccggct atgcgatggc cagttgggac accggctacg gcgatatggt gatgacgccg gacttgtcca ctctgcggct gattccttgg ctaccgggaa cggcgctggt gatcgccgac ctggtctggg ccgacggcag cgaggtcgcc gtctcgccgc gcagcattct gcgccgtcag ctcgatcggc tcaaggcgcg cggactggtc gccgatgtgg ccaccgagct ggagttcatc gtgttcgacc agccgtatcg ccaggcatgg gccagcgggt atcgcgggct gaccccggcc agcgactaca acatcgacta cgcgatattg gcatcctcgc ggatggagcc gttgctgcgc gacatccggt tgggtatggc cggtgcgggt ctgcgattcg aggcggtcaa aggcgaatgc aacatgggcc agcaggagat cgggtttcgt tacgacgagg cgctggtcac ctgcgacaac ccatgcgatc tacaagaacg gcgccaagga aatcgccgac cagcacggca agagcctaac gttcatggcg aaatacgatg aacgcgaagg taatagctgt cacatccatg tctcgctgcg tggcacggat ggctccgcgg tgtttgccga cagtaacggg ccgcacggca tgtcgtcgat gttccgcagc ttcgtcgccg gccagttggc cacgttgcgc gaattcacgc tgtgctatgc gccgaccatt aactcctaca agcgatttgc cgatagcagt ttcgcgccga cggcgctggc ttgggggctg gacaatcgca cctgcgccct gcgggtggtt ggccacgggc aaaacatccg ggtcgaatgc cgggttcccg gcggtgatgt caaccagtac ctggcggtgg cggctctcat tgctggaggg ttgtacggta tcgagcgggg ccttcagctg cccgagccct gtgtcggcaa cgcctaccaa ggcgccgatg tcgaacggct gccggttacg ctggccgacg ccgcggtgct gttcgaggat tctgcgctgg tgcgcgaggc gttcggcgag gatgttgtcg cgcactacct gaacaacgcg cgtgtggagc tggcggcgtt caacgcggcg gtcaccgatt gggagaggat
Expected Protein Sequence
MAHHHHHHMT GPGSPPLAWT ELERLVAAGD VDTVIVAFTD MQGRLAGKRI SGRHFVDDIA TRGVECCSYL LAVDVDLNTV PGYAMASWDT GYGDMVMTPD LSTLRLIPWL PGTALVIADL VWADGSEVAV SPRSILRRQL DRLKARGLVA DVATELEFIV FDQPYRQAWA SGYRGLTPAS DYNIDYAILA SSRMEPLLRD IRLGMAGAGL RFEAVKGECN MGQQEIGFRY DEALVTCDNH AIYKNGAKEI ADQHGKSLTF MAKYDEREGN SCHIHVSLRG TDGSAVFADS NGPHGMSSMF RSFVAGQLAT LREFTLCYAP TINSYKRFAD SSFAPTALAW GLDNRTCALR VVGHGQNIRV ECRVPGGDVN QYLAVAALIA GGLYGIERGL QLPEPCVGNA YQGADVERLP VTLADAAVLF EDSALVREAF GEDVVAHYLN NARVELAAFN AAVTDWERIR GFERL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgaccg gccccggttc gccgccgttg gcgtggaccg agttggagcg actggtcgcg gccggtgacg tcgacaccgt catcgtcgcg ttcaccgaca tgcagggccg gctggccggc aaacggatat cgggccggca tttcgtcgac gacatagcca cccgcggcgt cgagtgctgc agttatctgc tggccgtgga cgtcgacctg aacacggtgc ccggctatgc gatggccagt tgggacaccg gctacggcga tatggtgatg acgccggact tgtccactct gcggctgatt ccttggctac cgggaacggc gctggtgatc gccgacctgg tctgggccga cggcagcgag gtcgccgtct cgccgcgcag cattctgcgc cgtcagctcg atcggctcaa ggcgcgcgga ctggtcgccg atgtggccac cgagctggag ttcatcgtgt tcgaccagcc gtatcgccag gcatgggcca gcgggtatcg cgggctgacc ccggccagcg actacaacat cgactacgcg atattggcat cctcgcggat ggagccgttg ctgcgcgaca tccggttggg tatggccggt gcgggtctgc gattcgaggc ggtcaaaggc gaatgcaaca tgggccagca ggagatcggg tttcgttacg acgaggcgct ggtcacctgc gacaaccatg cgatctacaa gaacggcgcc aaggaaatcg ccgaccagca cggcaagagc ctaacgttca tggcgaaata cgatgaacgc gaaggtaata gctgtcacat ccatgtctcg ctgcgtggca cggatggctc cgcggtgttt gccgacagta acgggccgca cggcatgtcg tcgatgttcc gcagcttcgt cgccggccag ttggccacgt tgcgcgaatt cacgctgtgc tatgcgccga ccattaactc ctacaagcga tttgccgata gcagtttcgc gccgacggcg ctggcttggg ggctggacaa tcgcacctgc gccctgcggg tggttggcca cgggcaaaac atccgggtcg aatgccgggt tcccggcggt gatgtcaacc agtacctggc ggtggcggct ctcattgctg gagggttgta cggtatcgag cggggccttc agctgcccga gccctgtgtc ggcaacgcct accaaggcgc cgatgtcgaa cggctgccgg ttacgctggc cgacgccgcg gtgctgttcg aggattctgc gctggtgcgc gaggcgttcg gcgaggatgt tgtcgcgcac tacctgaaca acgcgcgtgt ggagctggcg gcgttcaacg cggcggtcac cgattgggag aggatacgtg gatttgagcg cctctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for MytuD.00111.a.HX1.GE43821
HARVESTED ON: 8/24/2020
SEQUENCED ON: 8/28/2020
EXPECTED MW: 107kDa
OBSERVED MW: 107kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMK NKEILAVVEA VSNEKALPRE KIFEALESAL ATATKKKYEQ EIDVRVQIDR KSGDFDTFRR WLVVDEVTQP TKEITLEAAR YEDESLNLGD YVEDQIESVT FDRITTQTAK QVIVQKVREA ERAMVVDQFR EHEGEIITGV VKKVNRDNIS LDLGNNAEAV ILREDMLPRE NFRPGDRVRG VLYSVRPEAR GAQLFVTRSK PEMLIELFRI EVPEIGEEVI EIKAAARDPG SRAKIAVKTN DKRIDPVGAC VGMRGARVQA VSTELGGERI DIVLWDDNPA QFVINAMAPA DVASIVVDED KHTMDIAVEA GNLAQAIGRN GQNVRLASQL SGWELNVMTV DDLQAKHQAE AHAAIDTFTK YLDIDEDFAT VLVEEGFSTL EELAYVPMKE LLEIEGLDEP TVEALRERAK NALATIAQAQ EESLGDNKPA DDLLNLEGVD RDLAFKLAAR GVCTLEDLAE QGIDDLADIE GLTDEKAGAL IMAARNICWF GDEAGTLEAQ TQGPGSMTGP GSPPLAWTEL ERLVAAGDVD TVIVAFTDMQ GRLAGKRISG RHFVDDIATR GVECCSYLLA VDVDLNTVPG YAMASWDTGY GDMVMTPDLS TLRLIPWLPG TALVIADLVW ADGSEVAVSP RSILRRQLDR LKARGLVADV ATELEFIVFD QPYRQAWASG YRGLTPASDY NIDYAILASS RMEPLLRDIR LGMAGAGLRF EAVKGECNMG QQEIGFRYDE ALVTCDNHAI YKNGAKEIAD QHGKSLTFMA KYDEREGNSC HIHVSLRGTD GSAVFADSNG PHGMSSMFRS FVAGQLATLR EFTLCYAPTI NSYKRFADSS FAPTALAWGL DNRTCALRVV GHGQNIRVEC RVPGGDVNQY LAVAALIAGG LYGIERGLQL PEPCVGNAYQ GADVERLPVT LADAAVLFED SALVREAFGE DVVAHYLNNA RVELAAFNAA VTDWERIRGF ERL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgaagaacaa agaaattttg gctgtagttg aagccgtatc caatgaaaag gcgctacctc gcgagaagat tttcgaagca ttggaaagcg cgctggcgac agcaacaaag aaaaaatatg aacaagagat cgacgtccgc gtacagatcg atcgcaaaag cggtgatttt gacactttcc gtcgctggtt agttgttgat gaagtcaccc agccgaccaa ggaaatcacc cttgaagccg cacgttatga agatgaaagc ctgaacctgg gcgattacgt tgaagatcag attgagtctg ttacctttga ccgtatcact acccagacgg caaaacaggt tatcgtgcag aaagtgcgtg aagccgaacg tgcgatggtg gttgatcagt tccgtgaaca cgaaggtgaa atcatcaccg gcgtggtgaa aaaagtaaac cgcgacaaca tctctctgga tctgggcaac aacgctgaag ccgtgatcct gcgcgaagat atgctgccgc gtgaaaactt ccgccctggc gaccgcgttc gtggcgtgct ctattccgtt cgcccggaag cgcgtggcgc gcaactgttc gtcactcgtt ccaagccgga aatgctgatc gaactgttcc gtattgaagt gccagaaatc ggcgaagaag tgattgaaat taaagcagcg gctcgcgatc cgggttctcg tgcgaaaatc gcggtgaaaa ccaacgataa acgtatcgat ccggtaggtg cttgcgtagg tatgcgtggc gcgcgtgttc aggcggtgtc tactgaactg ggtggcgagc gtatcgatat cgtcctgtgg gatgataacc cggcgcagtt cgtgattaac gcaatggcac cggcagacgt tgcttctatc gtggtggatg aagataaaca caccatggat atcgccgttg aagccggtaa cctggcgcag gcgattggcc gtaacggtca gaacgtgcgt ctggcttcgc agctgagcgg ttgggaactc aacgtgatga ccgttgacga cctgcaggct aagcatcagg cggaagcgca cgcagcgatc gacaccttca ccaaatatct cgacatcgac gaagacttcg cgactgttct ggtagaagaa ggcttctcga cgctggaaga attggcctat gtgccgatga aagagctgtt ggaaatcgaa ggccttgatg agccgaccgt tgaagcactg cgcgagcgtg ctaaaaatgc actggccacc attgcacagg cccaggaaga aagcctcggt gataacaaac cggctgacga tctgctgaac cttgaagggg tagatcgtga tttggcattc aaactggccg cccgtggcgt ttgtacgctg gaagatctcg ccgaacaggg cattgatgat ctggctgata tcgaagggtt gaccgacgaa aaagccggag cactgattat ggctgcccgt aacatttgct ggttcggtga cgaagcgggt accctggaag ctcagaccca gggtcctggt tcggtgaccg gccccggttc gccgccgttg gcgtggaccg agttggagcg actggtcgcg gccggtgacg tcgacaccgt catcgtcgcg ttcaccgaca tgcagggccg gctggccggc aaacggatat cgggccggca tttcgtcgac gacatagcca cccgcggcgt cgagtgctgc agttatctgc tggccgtgga cgtcgacctg aacacggtgc ccggctatgc gatggccagt tgggacaccg gctacggcga tatggtgatg acgccggact tgtccactct gcggctgatt ccttggctac cgggaacggc gctggtgatc gccgacctgg tctgggccga cggcagcgag gtcgccgtct cgccgcgcag cattctgcgc cgtcagctcg atcggctcaa ggcgcgcgga ctggtcgccg atgtggccac cgagctggag ttcatcgtgt tcgaccagcc gtatcgccag gcatgggcca gcgggtatcg cgggctgacc ccggccagcg actacaacat cgactacgcg atattggcat cctcgcggat ggagccgttg ctgcgcgaca tccggttggg tatggccggt gcgggtctgc gattcgaggc ggtcaaaggc gaatgcaaca tgggccagca ggagatcggg tttcgttacg acgaggcgct ggtcacctgc gacaaccatg cgatctacaa gaacggcgcc aaggaaatcg ccgaccagca cggcaagagc ctaacgttca tggcgaaata cgatgaacgc gaaggtaata gctgtcacat ccatgtctcg ctgcgtggca cggatggctc cgcggtgttt gccgacagta acgggccgca cggcatgtcg tcgatgttcc gcagcttcgt cgccggccag ttggccacgt tgcgcgaatt cacgctgtgc tatgcgccga ccattaactc ctacaagcga tttgccgata gcagtttcgc gccgacggcg ctggcttggg ggctggacaa tcgcacctgc gccctgcggg tggttggcca cgggcaaaac atccgggtcg aatgccgggt tcccggcggt gatgtcaacc agtacctggc ggtggcggct ctcattgctg gagggttgta cggtatcgag cggggccttc agctgcccga gccctgtgtc ggcaacgcct accaaggcgc cgatgtcgaa cggctgccgg ttacgctggc cgacgccgcg gtgctgttcg aggattctgc gctggtgcgc gaggcgttcg gcgaggatgt tgtcgcgcac tacctgaaca acgcgcgtgt ggagctggcg gcgttcaacg cggcggtcac cgattgggag aggatacgtg gatttgagcg cctcaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for MytuD.00111.a.MB1.GE43822
HARVESTED ON: 8/24/2020
SEQUENCED ON: 8/28/2020
EXPECTED MW: 95kDa
OBSERVED MW: 95kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 28
PERCENT COVERAGE: 28
Validated AA Sequence
MEPLLRDIRV GYGXCGSAIR GGQRRMQXWA SRRSGFVTTR RWSLRQPCDL QERRQGNRRP ARQEPNVHGE IRXNAKVIAV TSMSRCVARM APRCLPTVTG RTACRRCSAA SSPASWPRCA NSRCAMRRPL TPTSDLPIAV SRRRRWLGGW TIAPAPCGWL ATGKTSGSNA GFPAVMSTST WRWRLSLLEG CTVSSGAFSC PSPVSATPTK APMSNGCRLR WPTPRCCSRI LRWCARRSAR MLSRTT
Validated NT Sequence
ttnnnnnnnn nnggnnnnnn gttagcagcc ggatcctcga gaagcttggc tgcagaactt gttcgtgctg tttattagag gcgctcaaat ccacgtatcc tctcccaatc ggtgaccgcc gcgttgaacg ccgccagctc cacacgcgcg ttgttcaggt agtgcgcgac aacatcctcg ccgaacgcct cgcgcaccag cgcagaatcc tcgaacagca ccgcggcgtc ggccagcgta accggcagcc gttcgacatc ggcgccttgg taggcgttgc cgacacaggg ctcgggcagc tgaaggcccc gctcgatacc gtacaaccct ccagcaatga gagccgccac cgccaggtac tggttgacat caccgccggg aacccggcat tcgacccgga tgttttgccc gtggccaacc acccgcaggg cgcaggtgcg attgtccagc ccccaagcca gcgccgtcgg cgcgaaactg ctatcggcaa atcgcttgta ggagttaatg gtcggcgcat agcacagcgt gaattcgcgc aacgtggcca actggccggc gacgaagctg cggaacatcg acgacatgcc gtgcggcccg ttactgtcgg caaacaccgc ggagccatcc gtgccacgca gcgagacatg gatgtgacag ctattacctt cgcgttncat cgtatttcgc catgaacgtt aggctcttgc cgtgctggtc ggcgatttcc ttggcgccgt tcttgtagat cgcatggttg tcgcagtgac cagcgcctcg tcgtaacgaa acccgatctc ctgctggccc atgnttgcat tcgcctttga ccgcctcgaa tcgcagancc gcacnngcca tacccaaccc ggatgtcgcg cagcaacggc tccatnccgc ngaggatgnc atatcgcgta ntcgatgttg nagtcgctng gnggggtcan nnccgcgata cccgctggnc catgcctggn ggatacggnt gggtcgaacn acgatgaact tncancntcg gnggcnnnat tcggaacaag ttccgcggcn ccttganccg atcgaanctg angnnnnana anngnnnnnn nnnnggnnnn nanggnnacn tnnnntgncn gtnnngcnnn nnggnnaatn nnnnnnnnnn nnnnnccngn nnnncnngna ancncnnann nnnnnnna
Expected Protein Sequence
MAHHHHHHMK IEEGKLVIWI NGDKGYNGLA EVGKKFEKDT GIKVTVEHPD KLEEKFPQVA ATGDGPDIIF WAHDRFGGYA QSGLLAEITP DKAFQDKLYP FTWDAVRYNG KLIAYPIAVE ALSLIYNKDL LPNPPKTWEE IPALDKELKA KGKSALMFNL QEPYFTWPLI AADGGYAFKY ENGKYDIKDV GVDNAGAKAG LTFLVDLIKN KHMNADTDYS IAEAAFNKGE TAMTINGPWA WSNIDTSKVN YGVTVLPTFK GQPSKPFVGV LSAGINAASP NKELAKEFLE NYLLTDEGLE AVNKDKPLGA VALKSYEEEL AKDPRIAATM ENAQKGEIMP NIPQMSAFWY AVRTAVINAA SGRQTVDEAL KDAQTNSSSN NNNNNNNNNL GIEGRISEGT LEAQTQGPGS MTGPGSPPLA WTELERLVAA GDVDTVIVAF TDMQGRLAGK RISGRHFVDD IATRGVECCS YLLAVDVDLN TVPGYAMASW DTGYGDMVMT PDLSTLRLIP WLPGTALVIA DLVWADGSEV AVSPRSILRR QLDRLKARGL VADVATELEF IVFDQPYRQA WASGYRGLTP ASDYNIDYAI LASSRMEPLL RDIRLGMAGA GLRFEAVKGE CNMGQQEIGF RYDEALVTCD NHAIYKNGAK EIADQHGKSL TFMAKYDERE GNSCHIHVSL RGTDGSAVFA DSNGPHGMSS MFRSFVAGQL ATLREFTLCY APTINSYKRF ADSSFAPTAL AWGLDNRTCA LRVVGHGQNI RVECRVPGGD VNQYLAVAAL IAGGLYGIER GLQLPEPCVG NAYQGADVER LPVTLADAAV LFEDSALVRE AFGEDVVAHY LNNARVELAA FNAAVTDWER IRGFERL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcaca tgaaaatcga agaaggtaaa ctggtaatct ggattaacgg cgataaaggc tataacggtc tcgctgaagt cggtaagaaa ttcgagaaag ataccggaat taaagtcacc gttgagcatc cggataaact ggaagagaaa ttcccacagg ttgcggcaac tggcgatggc cctgacatta tcttctgggc acacgaccgc tttggtggct acgctcaatc tggcctgttg gctgaaatca ccccggacaa agcgttccag gacaagctgt atccgtttac ctgggatgcc gtacgttaca acggcaagct gattgcttac ccgatcgctg ttgaagcgtt atcgctgatt tataacaaag atctgctgcc gaacccgcca aaaacctggg aagagatccc ggcgctggat aaagaactga aagcgaaagg taagagcgcg ctgatgttca acctgcaaga accgtacttc acctggccgc tgattgctgc tgacgggggt tatgcgttca agtatgaaaa cggcaagtac gacattaaag acgtgggcgt ggataacgct ggcgcgaaag cgggtctgac cttcctggtt gacctgatta aaaacaaaca catgaatgca gacaccgatt actccatcgc agaagctgcc tttaataaag gcgaaacagc gatgaccatc aacggcccgt gggcatggtc caacatcgac accagcaaag tgaattatgg tgtaacggta ctgccgacct tcaagggtca accatccaaa ccgttcgttg gcgtgctgag cgcaggtatt aacgccgcca gtccgaacaa agagctggca aaagagttcc tcgaaaacta tctgctgact gatgaaggtc tggaagcggt taataaagac aaaccgctgg gtgccgtagc gctgaagtct tacgaggaag agttggcgaa agatccacgt attgccgcca ctatggaaaa cgcccagaaa ggtgaaatca tgccgaacat cccgcagatg tccgctttct ggtatgccgt gcgtactgcg gtgatcaacg ccgccagcgg tcgtcagact gtcgatgaag ccctgaaaga cgcgcagact aattcgagct cgaacaacaa caacaacaac aataacaaca acctcgggat cgagggaagg atttcagaag gtaccctgga agctcagacc cagggtcctg gttcggtgac cggccccggt tcgccgccgt tggcgtggac cgagttggag cgactggtcg cggccggtga cgtcgacacc gtcatcgtcg cgttcaccga catgcagggc cggctggccg gcaaacggat atcgggccgg catttcgtcg acgacatagc cacccgcggc gtcgagtgct gcagttatct gctggccgtg gacgtcgacc tgaacacggt gcccggctat gcgatggcca gttgggacac cggctacggc gatatggtga tgacgccgga cttgtccact ctgcggctga ttccttggct accgggaacg gcgctggtga tcgccgacct ggtctgggcc gacggcagcg aggtcgccgt ctcgccgcgc agcattctgc gccgtcagct cgatcggctc aaggcgcgcg gactggtcgc cgatgtggcc accgagctgg agttcatcgt gttcgaccag ccgtatcgcc aggcatgggc cagcgggtat cgcgggctga ccccggccag cgactacaac atcgactacg cgatattggc atcctcgcgg atggagccgt tgctgcgcga catccggttg ggtatggccg gtgcgggtct gcgattcgag gcggtcaaag gcgaatgcaa catgggccag caggagatcg ggtttcgtta cgacgaggcg ctggtcacct gcgacaacca tgcgatctac aagaacggcg ccaaggaaat cgccgaccag cacggcaaga gcctaacgtt catggcgaaa tacgatgaac gcgaaggtaa tagctgtcac atccatgtct cgctgcgtgg cacggatggc tccgcggtgt ttgccgacag taacgggccg cacggcatgt cgtcgatgtt ccgcagcttc gtcgccggcc agttggccac gttgcgcgaa ttcacgctgt gctatgcgcc gaccattaac tcctacaagc gatttgccga tagcagtttc gcgccgacgg cgctggcttg ggggctggac aatcgcacct gcgccctgcg ggtggttggc cacgggcaaa acatccgggt cgaatgccgg gttcccggcg gtgatgtcaa ccagtacctg gcggtggcgg ctctcattgc tggagggttg tacggtatcg agcggggcct tcagctgccc gagccctgtg tcggcaacgc ctaccaaggc gccgatgtcg aacggctgcc ggttacgctg gccgacgccg cggtgctgtt cgaggattct gcgctggtgc gcgaggcgtt cggcgaggat gttgtcgcgc actacctgaa caacgcgcgt gtggagctgg cggcgttcaa cgcggcggtc accgattggg agaggatacg tggatttgag cgcctctaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa