MytuD.00095.b

Putative non-heme bromoperoxidase BpoC (EC 1.11.1.18) (Rv0554)

CENTER ID: MytuD.00095.b
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: in PDB
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:
IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
MytuD.00095.b.B1.GE43817 full length 1 262
MytuD.00095.b.HX1.GE43818 full length 1 262
MytuD.00095.b.MB1.GE43819 full length 1 262
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
Structures
7LD8
DEPOSITED: 1/12/2021
DETERMINATION: XRay
CLONE: MytuD.00095.b.B1.GE43817
PROTEIN: MytuD.00095.b.B1.PW38899
External Resources
RESOURCE REFERENCE ID
PATRIC ID: fig|83332.12.peg.614
TubercuList: Rv0554
MTB Network Portal: Rv0554
UniProt: P9WNH1
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MINLAYDDNG TGDPVVFIAG RGGAGRTWHP HQVPAFLAAG YRCITFDNRG IGATENAEGF TTQTMVADTA ALIETLDIAP ARVVGVSMGA FIAQELMVVA PELVSSAVLM ATRGRLDRAR QFFNKAEAEL YDSGVQLPPT YDARARLLEN FSRKTLNDDV AVGDWIAMFS MWPIKSTPGL RCQLDCAPQT NRLPAYRNIA APVLVIGFAD DVVTPPYLGR EVADALPNGR YLQIPDAGHL GFFERPEAVN TAMLKFFASV KA
NT Sequence
gtgatcaacc tggcctacga cgacaacggg accggtgacc cggtggtctt tatcgccggc cgcggcggcg ccggacgcac ctggcaccca catcaagtcc cggcctttct ggcggctgga tatcggtgca tcacgttcga caatcggggc atcggcgcca ccgaaaacgc cgaaggcttc accacgcaaa ccatggtcgc cgacaccgcg gcgctgatcg aaaccctaga catcgccccg gcgcgcgttg tcggggtgtc gatgggggca ttcatcgcgc aggaactcat ggtggtcgca cccgagctgg tcagctcggc ggtgctgatg gccactcgcg gccgcctgga ccgcgcccgc cagttcttta acaaagccga ggccgaactc tatgactcgg gtgtccagct gccacccaca tacgacgcga gggctcgctt actggagaac ttctcccgaa agacgctcaa cgatgacgtg gccgttggcg actggatcgc gatgttttcc atgtggccga ttaagtccac ccccggactg cgctgtcagc tagattgcgc tccgcagacc aaccggctgc ccgcctaccg caacatcgcc gcgccggtgc tggtgattgg tttcgccgac gacgtggtga cgccgcccta cctgggtcgg gaggtcgccg acgccctgcc gaacggccgt tacctgcaga tacctgacgc cggtcatctc gggttcttcg agcggccgga agccgtcaac accgcgatgc tgaagttctt cgccagtgtc aaggcctga
Details for MytuD.00095.b.B1.GE43817
HARVESTED ON: 8/24/2020
SEQUENCED ON: 8/28/2020
EXPECTED MW: 29kDa
OBSERVED MW: 29kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 97
PERCENT COVERAGE: 90
Validated AA Sequence
MAHHHHHMVI NLAYDDNGTG DPVVFIAGRG GAGRTWHPHQ VPAFLAAGYR CITFDNRGIG ATENAEGFTT QTMVADTAAL IETLDIAPAR VVGVSMGAFI AQELMVVAPE LVSSAVLMAT RGRLDRARQF FNKAEAELYD SGVQLPPTYD ARARLLENFS RKTLNDDVAV GDWIAMFSMW PIKSTPGLRC QLDCAPQTYR LPAYRNIAAP VLVIGFADDV VTPPYLGREV ADALPNGPLP ADT
Validated NT Sequence
atggctcacc accaccacca tatggtgatc aacctggcct acgacgacaa cgggaccggt gacccggtgg tctttatcgc cggccgcggc ggcgccggac gcacctggca cccacatcaa gtcccggcct ttctggcggc tggatatcgg tgcatcacgt tcgacaatcg gggcatcggc gccaccgaaa acgccgaagg cttcaccacg caaaccatgg tcgccgacac cgcggcgctg atcgaaaccc tagacatcgc cccggcgcgc gttgtcgggg tgtcgatggg ggcattcatc gcgcaggaac tcatggtggt cgcacccgag ctggtcagct cggcggtgct gatggccact cgcggccgcc tggaccgcgc ccgccagttc tttaacaaag ccgaggccga actctatgac tcgggtgtcc agctgccacc cacatacgac gcgagggctc gcttactgga gaacttctcc cgaaagacgc tcaacgatga cgtggccgtt ggcgactgga tcgcgatgtt ttccatgtgg ccgattaagt ccacccccgg actgcgctgt cagctagatt gcgctccgca gacctaccgg ctgcccgcct accgcaacat cgccgcgccg gtgctggtga ttggtttcgc cgacgacgtg gtgacgccgc cctacctggg tcgggaggtc gccgacgccc tgccgaacgg gccgttacct gcagatacct gacgccggtc atctcgggtt cttcgagcgg ccggaagccg tcaacaccgc gatgctgaag tncttcgcca gtgtcaaggc ctaagtgagt aagat
Expected Protein Sequence
MAHHHHHHMI NLAYDDNGTG DPVVFIAGRG GAGRTWHPHQ VPAFLAAGYR CITFDNRGIG ATENAEGFTT QTMVADTAAL IETLDIAPAR VVGVSMGAFI AQELMVVAPE LVSSAVLMAT RGRLDRARQF FNKAEAELYD SGVQLPPTYD ARARLLENFS RKTLNDDVAV GDWIAMFSMW PIKSTPGLRC QLDCAPQTNR LPAYRNIAAP VLVIGFADDV VTPPYLGREV ADALPNGRYL QIPDAGHLGF FERPEAVNTA MLKFFASVKA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgatca acctggccta cgacgacaac gggaccggtg acccggtggt ctttatcgcc ggccgcggcg gcgccggacg cacctggcac ccacatcaag tcccggcctt tctggcggct ggatatcggt gcatcacgtt cgacaatcgg ggcatcggcg ccaccgaaaa cgccgaaggc ttcaccacgc aaaccatggt cgccgacacc gcggcgctga tcgaaaccct agacatcgcc ccggcgcgcg ttgtcggggt gtcgatgggg gcattcatcg cgcaggaact catggtggtc gcacccgagc tggtcagctc ggcggtgctg atggccactc gcggccgcct ggaccgcgcc cgccagttct ttaacaaagc cgaggccgaa ctctatgact cgggtgtcca gctgccaccc acatacgacg cgagggctcg cttactggag aacttctccc gaaagacgct caacgatgac gtggccgttg gcgactggat cgcgatgttt tccatgtggc cgattaagtc cacccccgga ctgcgctgtc agctagattg cgctccgcag accaaccggc tgcccgccta ccgcaacatc gccgcgccgg tgctggtgat tggtttcgcc gacgacgtgg tgacgccgcc ctacctgggt cgggaggtcg ccgacgccct gccgaacggc cgttacctgc agatacctga cgccggtcat ctcgggttct tcgagcggcc ggaagccgtc aacaccgcga tgctgaagtt cttcgccagt gtcaaggcct gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for MytuD.00095.b.HX1.GE43818
HARVESTED ON: 8/24/2020
SEQUENCED ON: 8/28/2020
EXPECTED MW: 85kDa
OBSERVED MW: 85kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 96
PERCENT COVERAGE: 33
Validated AA Sequence
MVINXXTTTN ETGDPVVFIA GRGGAGRTXH PHQVPAFLAA GYRCITFDNR GIGATENAEG FTTQTMVADT AALIETLDIA PARVVGVSMG AFIAQELMVV APELVSSAVL MATRGRLDRA RQFFNKAEAE LYDSGVQLPP TYDARARLLE NFSRKTLNDD VAVGDWIAMF SMWPIKSTPG LRCQLDCAPQ TNRLPAYRNI AAPVLVIGFA DDVVTPPYLG REVADALPNG RYLQIPDAGH LGFFERPEAV NTAMLKFFAS VKA
Validated NT Sequence
ggnnnnnngt tagcagccgg atcctcgaga agcttggctg cagaacttgt tcgtgctgtt tattaggcct tgacactggc gaagaacttc agcatcgcgg tgttgacggc ttccggccgc tcgaagaacc cgagatgacc ggcgtcaggt atctgcaggt aacggccgtt cggcagggcg tcggcgacct cccgacccag gtagggcggc gtcaccacgt cgtcggcgaa accaatcacc agcaccggcg cggcgatgtt gcggtaggcg ggcagccggt tggtctgcgg agcgcaatct agctgacagc gcagtccggg ggtggactta atcggccaca tggaaaacat cgcgatccag tcgccaacgg ccacgtcatc gttgagcgtc tttcgggaga agttctccag taagcgagcc ctcgcgtcgt atgtgggtgg cagctggaca cccgagtcat agagttcggc ctcggctttg ttaaagaact ggcgggcgcg gtccaggcgg ccgcgagtgg ccatcagcac cgccgagctg accagctcgg gtgcgaccac catgagttcc tgcgcgatga atgcccccat cgacaccccg acaacgcgcg ccggggcgat gtctagggtt tcgatcagcg ccgcggtgtc ggcgaccatg gtttgcgtgg tgaagccttc ggcgttttcg gtggcgccga tgccccgatt gtcgaacgtg atgcaccgat atccagccgc cagaaaggcc gggacttgat gtgggtgnca ggtgcgtccg gcgccgccgc ggccggcgat aaagaccacc gggtcaccgg tctcgttngt cgtcgtngnc angttgatca ccatcgaacc agnaccctgg gtctgagctt tcagggtacc ccgcttcgtc accnaaccag caaatgttac gggcagccat aatcagngct ccnggctttt ttcgtcggnc aaccctttcg annatcagcc agatnntcaa nngccctgtt tcgngaagat tctttccnnc gtannnaaac nncnnngggn ggnccagttt gaatgnnnaa tcncgatcnn ncccnncann ntnnagnnna tcgtcannnn ntnnntnnnn nnncgnangn ttttntnngn nnnnnnngcn anngggnggn nnngnnannt nnnnnnnnnn ntnnnnnngn gnnttncaan gggnncnnnn nnc
Expected Protein Sequence
MAHHHHHHMK NKEILAVVEA VSNEKALPRE KIFEALESAL ATATKKKYEQ EIDVRVQIDR KSGDFDTFRR WLVVDEVTQP TKEITLEAAR YEDESLNLGD YVEDQIESVT FDRITTQTAK QVIVQKVREA ERAMVVDQFR EHEGEIITGV VKKVNRDNIS LDLGNNAEAV ILREDMLPRE NFRPGDRVRG VLYSVRPEAR GAQLFVTRSK PEMLIELFRI EVPEIGEEVI EIKAAARDPG SRAKIAVKTN DKRIDPVGAC VGMRGARVQA VSTELGGERI DIVLWDDNPA QFVINAMAPA DVASIVVDED KHTMDIAVEA GNLAQAIGRN GQNVRLASQL SGWELNVMTV DDLQAKHQAE AHAAIDTFTK YLDIDEDFAT VLVEEGFSTL EELAYVPMKE LLEIEGLDEP TVEALRERAK NALATIAQAQ EESLGDNKPA DDLLNLEGVD RDLAFKLAAR GVCTLEDLAE QGIDDLADIE GLTDEKAGAL IMAARNICWF GDEAGTLEAQ TQGPGSMINL AYDDNGTGDP VVFIAGRGGA GRTWHPHQVP AFLAAGYRCI TFDNRGIGAT ENAEGFTTQT MVADTAALIE TLDIAPARVV GVSMGAFIAQ ELMVVAPELV SSAVLMATRG RLDRARQFFN KAEAELYDSG VQLPPTYDAR ARLLENFSRK TLNDDVAVGD WIAMFSMWPI KSTPGLRCQL DCAPQTNRLP AYRNIAAPVL VIGFADDVVT PPYLGREVAD ALPNGRYLQI PDAGHLGFFE RPEAVNTAML KFFASVKA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgaagaacaa agaaattttg gctgtagttg aagccgtatc caatgaaaag gcgctacctc gcgagaagat tttcgaagca ttggaaagcg cgctggcgac agcaacaaag aaaaaatatg aacaagagat cgacgtccgc gtacagatcg atcgcaaaag cggtgatttt gacactttcc gtcgctggtt agttgttgat gaagtcaccc agccgaccaa ggaaatcacc cttgaagccg cacgttatga agatgaaagc ctgaacctgg gcgattacgt tgaagatcag attgagtctg ttacctttga ccgtatcact acccagacgg caaaacaggt tatcgtgcag aaagtgcgtg aagccgaacg tgcgatggtg gttgatcagt tccgtgaaca cgaaggtgaa atcatcaccg gcgtggtgaa aaaagtaaac cgcgacaaca tctctctgga tctgggcaac aacgctgaag ccgtgatcct gcgcgaagat atgctgccgc gtgaaaactt ccgccctggc gaccgcgttc gtggcgtgct ctattccgtt cgcccggaag cgcgtggcgc gcaactgttc gtcactcgtt ccaagccgga aatgctgatc gaactgttcc gtattgaagt gccagaaatc ggcgaagaag tgattgaaat taaagcagcg gctcgcgatc cgggttctcg tgcgaaaatc gcggtgaaaa ccaacgataa acgtatcgat ccggtaggtg cttgcgtagg tatgcgtggc gcgcgtgttc aggcggtgtc tactgaactg ggtggcgagc gtatcgatat cgtcctgtgg gatgataacc cggcgcagtt cgtgattaac gcaatggcac cggcagacgt tgcttctatc gtggtggatg aagataaaca caccatggat atcgccgttg aagccggtaa cctggcgcag gcgattggcc gtaacggtca gaacgtgcgt ctggcttcgc agctgagcgg ttgggaactc aacgtgatga ccgttgacga cctgcaggct aagcatcagg cggaagcgca cgcagcgatc gacaccttca ccaaatatct cgacatcgac gaagacttcg cgactgttct ggtagaagaa ggcttctcga cgctggaaga attggcctat gtgccgatga aagagctgtt ggaaatcgaa ggccttgatg agccgaccgt tgaagcactg cgcgagcgtg ctaaaaatgc actggccacc attgcacagg cccaggaaga aagcctcggt gataacaaac cggctgacga tctgctgaac cttgaagggg tagatcgtga tttggcattc aaactggccg cccgtggcgt ttgtacgctg gaagatctcg ccgaacaggg cattgatgat ctggctgata tcgaagggtt gaccgacgaa aaagccggag cactgattat ggctgcccgt aacatttgct ggttcggtga cgaagcgggt accctggaag ctcagaccca gggtcctggt tcggtgatca acctggccta cgacgacaac gggaccggtg acccggtggt ctttatcgcc ggccgcggcg gcgccggacg cacctggcac ccacatcaag tcccggcctt tctggcggct ggatatcggt gcatcacgtt cgacaatcgg ggcatcggcg ccaccgaaaa cgccgaaggc ttcaccacgc aaaccatggt cgccgacacc gcggcgctga tcgaaaccct agacatcgcc ccggcgcgcg ttgtcggggt gtcgatgggg gcattcatcg cgcaggaact catggtggtc gcacccgagc tggtcagctc ggcggtgctg atggccactc gcggccgcct ggaccgcgcc cgccagttct ttaacaaagc cgaggccgaa ctctatgact cgggtgtcca gctgccaccc acatacgacg cgagggctcg cttactggag aacttctccc gaaagacgct caacgatgac gtggccgttg gcgactggat cgcgatgttt tccatgtggc cgattaagtc cacccccgga ctgcgctgtc agctagattg cgctccgcag accaaccggc tgcccgccta ccgcaacatc gccgcgccgg tgctggtgat tggtttcgcc gacgacgtgg tgacgccgcc ctacctgggt cgggaggtcg ccgacgccct gccgaacggc cgttacctgc agatacctga cgccggtcat ctcgggttct tcgagcggcc ggaagccgtc aacaccgcga tgctgaagtt cttcgccagt gtcaaggcca aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for MytuD.00095.b.MB1.GE43819
HARVESTED ON: 8/24/2020
SEQUENCED ON: 8/28/2020
EXPECTED MW: 73kDa
OBSERVED MW: 73kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 92
PERCENT COVERAGE: 39
Validated AA Sequence
MVINLAYDDN GTGDPVVFYR RPRRRRTHLG THIKSRPFWR AGYRCITFDX RGIGAXENAE GFTTQTMVAD TAALIETLDI APARVVGVSM GAFIAQELMV VAPELVSSAV LMATRGRLDR ARQFFNKAEA ELYDSGVQLP PTYDARARLL ENFSRKTLND DVAVGDWIAM FSMWPIKSTP GLRCQLDCAP QTNRLPAYRN IAAPVLVIGF ADDVVTPPYL GREVADALPN GRYLQIPDAG HLGFFERPEA VNTAMLKFFA SVKA
Validated NT Sequence
cnnnnnnnng ttagcagccg gatcctcgag aagcttggct gcagaacttg ttcgtgctgt ttattaggcc ttgacactgg cgaagaactt cagcatcgcg gtgttgacgg cttccggccg ctcgaagaac ccgagatgac cggcgtcagg tatctgcagg taacggccgt tcggcagggc gtcggcgacc tcccgaccca ggtagggcgg cgtcaccacg tcgtcggcga aaccaatcac cagcaccggc gcggcgatgt tgcggtaggc gggcagccgg ttggtctgcg gagcgcaatc tagctgacag cgcagtccgg gggtggactt aatcggccac atggaaaaca tcgcgatcca gtcgccaacg gccacgtcat cgttgagcgt ctttcgggag aagttctcca gtaagcgagc cctcgcgtcg tatgtgggtg gcagctggac acccgagtca tagagttcgg cctcggcttt gttaaagaac tggcgggcgc ggtccaggcg gccgcgagtg gccatcagca ccgccgagct gaccagctcg ggtgcgacca ccatgagttc ctgcgcgatg aatgccccca tcgacacccc gacaacgcgc gccggggcga tgtctagggt ttcgatcagc gccgcggtgt cggcgaccat ggtttgcgtg gtgaagcctt cggcgttttc ggnggcgccg atgccccgan tgtcgaacgt gatgcaccga tatccagccc gccagaaagg ccgggacttg atgtgggtgc cnaggtgcgt ccggcgccgc cgcggccggc gataaaagac caccgggtca ccggtcccgt tgtcgtcgta ggccaggttg atcaccatcg aancaggacc ctggggtctn nagcttccag ggtaccttct gaannccttt ccctcgatcc cgangttgtt nntgttgttg nngttgttgt tcnagcnnnn nttantcnnn nnncgtcttt cagggnttnn tanngannag nctgangacc gctggcggng nttgatcnnc cgcannacnc nnngnntann ngannngnnn ntctgnnngg angntcggna tgnattnnnn nnnnngnnnn nnnnnnnngg nnnnannnnn gnntntnnnn annnnnccnn nnnnnanntn anngntnngn nnnnccncnn nngg
Expected Protein Sequence
MAHHHHHHMK IEEGKLVIWI NGDKGYNGLA EVGKKFEKDT GIKVTVEHPD KLEEKFPQVA ATGDGPDIIF WAHDRFGGYA QSGLLAEITP DKAFQDKLYP FTWDAVRYNG KLIAYPIAVE ALSLIYNKDL LPNPPKTWEE IPALDKELKA KGKSALMFNL QEPYFTWPLI AADGGYAFKY ENGKYDIKDV GVDNAGAKAG LTFLVDLIKN KHMNADTDYS IAEAAFNKGE TAMTINGPWA WSNIDTSKVN YGVTVLPTFK GQPSKPFVGV LSAGINAASP NKELAKEFLE NYLLTDEGLE AVNKDKPLGA VALKSYEEEL AKDPRIAATM ENAQKGEIMP NIPQMSAFWY AVRTAVINAA SGRQTVDEAL KDAQTNSSSN NNNNNNNNNL GIEGRISEGT LEAQTQGPGS MINLAYDDNG TGDPVVFIAG RGGAGRTWHP HQVPAFLAAG YRCITFDNRG IGATENAEGF TTQTMVADTA ALIETLDIAP ARVVGVSMGA FIAQELMVVA PELVSSAVLM ATRGRLDRAR QFFNKAEAEL YDSGVQLPPT YDARARLLEN FSRKTLNDDV AVGDWIAMFS MWPIKSTPGL RCQLDCAPQT NRLPAYRNIA APVLVIGFAD DVVTPPYLGR EVADALPNGR YLQIPDAGHL GFFERPEAVN TAMLKFFASV KA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcaca tgaaaatcga agaaggtaaa ctggtaatct ggattaacgg cgataaaggc tataacggtc tcgctgaagt cggtaagaaa ttcgagaaag ataccggaat taaagtcacc gttgagcatc cggataaact ggaagagaaa ttcccacagg ttgcggcaac tggcgatggc cctgacatta tcttctgggc acacgaccgc tttggtggct acgctcaatc tggcctgttg gctgaaatca ccccggacaa agcgttccag gacaagctgt atccgtttac ctgggatgcc gtacgttaca acggcaagct gattgcttac ccgatcgctg ttgaagcgtt atcgctgatt tataacaaag atctgctgcc gaacccgcca aaaacctggg aagagatccc ggcgctggat aaagaactga aagcgaaagg taagagcgcg ctgatgttca acctgcaaga accgtacttc acctggccgc tgattgctgc tgacgggggt tatgcgttca agtatgaaaa cggcaagtac gacattaaag acgtgggcgt ggataacgct ggcgcgaaag cgggtctgac cttcctggtt gacctgatta aaaacaaaca catgaatgca gacaccgatt actccatcgc agaagctgcc tttaataaag gcgaaacagc gatgaccatc aacggcccgt gggcatggtc caacatcgac accagcaaag tgaattatgg tgtaacggta ctgccgacct tcaagggtca accatccaaa ccgttcgttg gcgtgctgag cgcaggtatt aacgccgcca gtccgaacaa agagctggca aaagagttcc tcgaaaacta tctgctgact gatgaaggtc tggaagcggt taataaagac aaaccgctgg gtgccgtagc gctgaagtct tacgaggaag agttggcgaa agatccacgt attgccgcca ctatggaaaa cgcccagaaa ggtgaaatca tgccgaacat cccgcagatg tccgctttct ggtatgccgt gcgtactgcg gtgatcaacg ccgccagcgg tcgtcagact gtcgatgaag ccctgaaaga cgcgcagact aattcgagct cgaacaacaa caacaacaac aataacaaca acctcgggat cgagggaagg atttcagaag gtaccctgga agctcagacc cagggtcctg gttcggtgat caacctggcc tacgacgaca acgggaccgg tgacccggtg gtctttatcg ccggccgcgg cggcgccgga cgcacctggc acccacatca agtcccggcc tttctggcgg ctggatatcg gtgcatcacg ttcgacaatc ggggcatcgg cgccaccgaa aacgccgaag gcttcaccac gcaaaccatg gtcgccgaca ccgcggcgct gatcgaaacc ctagacatcg ccccggcgcg cgttgtcggg gtgtcgatgg gggcattcat cgcgcaggaa ctcatggtgg tcgcacccga gctggtcagc tcggcggtgc tgatggccac tcgcggccgc ctggaccgcg cccgccagtt ctttaacaaa gccgaggccg aactctatga ctcgggtgtc cagctgccac ccacatacga cgcgagggct cgcttactgg agaacttctc ccgaaagacg ctcaacgatg acgtggccgt tggcgactgg atcgcgatgt tttccatgtg gccgattaag tccacccccg gactgcgctg tcagctagat tgcgctccgc agaccaaccg gctgcccgcc taccgcaaca tcgccgcgcc ggtgctggtg attggtttcg ccgacgacgt ggtgacgccg ccctacctgg gtcgggaggt cgccgacgcc ctgccgaacg gccgttacct gcagatacct gacgccggtc atctcgggtt cttcgagcgg ccggaagccg tcaacaccgc gatgctgaag ttcttcgcca gtgtcaaggc ctaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa