MytuD.00014.a

Isocitrate lyase (Rv0467)

CENTER ID: MytuD.00014.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.00014.a.B1.GE43499 full length 1 428
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.00014.a.B1.PW38797 full length 1 428

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.515
TubercuList: Rv0467
MTB Network Portal: Rv0467
UniProt: P9WKK7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSVVGTPKSA EQIQQEWDTN PRWKDVTRTY SAEDVVALQG SVVEEHTLAR RGAEVLWEQL HDLEWVNALG ALTGNMAVQQ VRAGLKAIYL SGWQVAGDAN LSGHTYPDQS LYPANSVPQV VRRINNALQR ADQIAKIEGD TSVENWLAPI VADGEAGFGG ALNVYELQKA LIAAGVAGSH WEDQLASEKK CGHLGGKVLI PTQQHIRTLT SARLAADVAD VPTVVIARTD AEAATLITSD VDERDQPFIT GERTREGFYR TKNGIEPCIA RAKAYAPFAD LIWMETGTPD LEAARQFSEA VKAEYPDQML AYNCSPSFNW KKHLDDATIA KFQKELAAMG FKFQFITLAG FHALNYSMFD LAYGYAQNQM SAYVELQERE FAAEERGYTA TKHQREVGAG YFDRIATTVD PNSSTTALTG STEEGQFH
NT Sequence
atgtctgtcg tcggcacccc gaagagcgcg gagcagatcc agcaggaatg ggacacgaac ccgcgctgga aggacgtcac ccgcacctac tccgccgagg acgtcgtcgc cctccagggc agcgtggtcg aggagcacac gctggcccgc cgcggtgcgg aggtgctgtg ggagcagctg cacgacctcg agtgggtcaa cgcgctgggc gcgctgaccg gcaacatggc cgtccagcag gtgcgcgccg gcctgaaggc catctacctg tcgggctggc aggtcgccgg cgatgccaac ctgtccgggc acacctaccc cgaccagagc ctgtatcccg ccaactcggt gccgcaggtg gtccgccgga tcaacaacgc actgcagcgc gccgaccaga tcgccaagat cgagggcgat acttcggtgg agaactggct ggcgccgatt gtcgccgacg gcgaggccgg ctttggcggc gcgctcaacg tctacgagct gcagaaagcc ctgatcgccg cgggcgttgc gggttcgcac tgggaggacc agttggcctc tgagaagaag tgcggccacc tgggcggcaa ggtgttgatc ccgacccagc agcacatccg cactttgacg tctgctcggc tcgcggccga tgtggctgat gttcccacgg tggtgatcgc ccgtaccgac gccgaggcgg ccacgctgat cacctccgac gtcgacgagc gcgaccagcc gttcatcacc ggcgagcgca cccgggaagg cttctaccgc accaagaacg gcatcgagcc ttgcatcgct cgggcgaagg cctacgcccc gttcgccgac ttgatctgga tggagaccgg taccccggac ctcgaggccg cccggcagtt ctccgaggcg gtcaaggcgg agtacccgga ccagatgctg gcctacaact gctcgccatc gttcaactgg aaaaagcacc tcgacgacgc caccatcgcc aagttccaga aggagctggc agccatgggc ttcaagttcc agttcatcac gctggccggc ttccatgcgc tgaactactc gatgttcgat ctggcctacg gctacgccca gaaccagatg agcgcgtatg tcgaactgca ggaacgcgag ttcgccgccg aagaacgggg ctacaccgcg accaagcacc agcgcgaggt cggcgccggc tacttcgacc ggattgccac caccgtggac ccgaattcgt cgaccaccgc gttgaccggt tccaccgaag agggccagtt ccactag
Details for MytuD.00014.a.B1.GE43499
HARVESTED ON: 11/19/2019
SEQUENCED ON: 12/3/2019
EXPECTED MW: 48kDa
OBSERVED MW: 48kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMS VVGTPKSAEQ IQQEWDTNPR WKDVTRTYSA EDVVALQGSV VEEHTLARRG AEVLWEQLHD LEWVNALGAL TGNMAVQQVR AGLKAIYLSG WQVAGDANLS GHTYPDQSLY PANSVPQVVR RINNALQRAD QIAKIEGDTS VENWLAPIVA DGEAGFGGAL NVYELQKALI AAGVAGSHWE DQLASEKKCG HLGGKVLIPT QQHIRTLTSA RLAADVADVP TVVIARTDAE AATLITSDVD ERDQPFITGE RTREGFYRTK NGIEPCIARA KAYAPFADLI WMETGTPDLE AARQFSEAVK AEYPDQMLAY NCSPSFNWKK HLDDATIAKF QKELAAMGFK FQFITLAGFH ALNYSMFDLA YGYAQNQMSA YVELQEREFA AEERGYTATK HQREVGAGYF DRIATTVDPN SSTTALTGS
Validated NT Sequence
ggtggaaccg gtcaacgcgg tggtcgacga attcgggtcc acggtggtgg caatccggtc gaagtagccg gcgccgacct cgcgctggtg cttggtcgcg gtgtagcccc gttcttcggc ggcgaactcg cgttcctgca gttcgacata cgcgctcatc tggttctggg cgtagccgta ggccagatcg aacatcgagt agttcagcgc atggaagccg gccagcgtga tgaactggaa cttgaagccc atggctgcca gctccttctg gaacttggcg atggtggcgt cgtcgaggtg ctttttccag ttgaacgatg gcgagcagtt gtaggccagc atctggtccg ggtactccgc cttgaccgcc tcggagaact gccgggcggc ctcgaggtcc ggggtaccgg tctccatcca gatcaagtcg gcgaacgggg cgtaggcctt cgcccgagcg atgcaaggct cgatgccgtt cttggtgcgg tagaagcctt cccgggtgcg ctcgccggtg atgaacggct ggtcgcgctc gtcgacgtcg gaggtgatca gcgtggccgc ctcggcgtcg gtacgggcga tcaccaccgt gggaacatca gccacatcgg ccgcgagccg agcagacgtc aaagtgcgga tgtgctgctg ggtcgggatc aacaccttgc cgcccaggtg gccgcacttc ttctcagagg ccaactggtc ctcccagtgc gaacccgcaa cgcccgcggc gatcagggct ttctgcagct cgtagacgtt gagcgcgccg ccaaagccgg cctcgccgtc ggcgacaatc ggcgccagcc agttctccac cgaagtatcg ccctcgatct tggcgatctg gtcggcgcgc tgcagtgcgt tgttgatccg gcggaccacc tgcggcaccg agttggcggg atacaggctc tggtcggggt aggtgtgccc ggacaggttg gcatcgccgg cgacctgcca gcccgacagg tagatggcct tcaggccggc gcgcacctgc tggacggcca tgttgccggt cagcgcgccc agcgcgttga cccactcgag gtcgtgcagc tgctcccaca gcacctccgc accgcggcgg gccagcgtgt gctcctcgac cacgctgccc tggagggcga cgacgtcctc ggcggagtag gtgcgggtga cgtccttcca gcgcgggttc gtgtcccatt cctgctggat ctgctccgcg ctcttcgggg tgccgacgac agacatatgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHMS VVGTPKSAEQ IQQEWDTNPR WKDVTRTYSA EDVVALQGSV VEEHTLARRG AEVLWEQLHD LEWVNALGAL TGNMAVQQVR AGLKAIYLSG WQVAGDANLS GHTYPDQSLY PANSVPQVVR RINNALQRAD QIAKIEGDTS VENWLAPIVA DGEAGFGGAL NVYELQKALI AAGVAGSHWE DQLASEKKCG HLGGKVLIPT QQHIRTLTSA RLAADVADVP TVVIARTDAE AATLITSDVD ERDQPFITGE RTREGFYRTK NGIEPCIARA KAYAPFADLI WMETGTPDLE AARQFSEAVK AEYPDQMLAY NCSPSFNWKK HLDDATIAKF QKELAAMGFK FQFITLAGFH ALNYSMFDLA YGYAQNQMSA YVELQEREFA AEERGYTATK HQREVGAGYF DRIATTVDPN SSTTALTGST EEGQFH
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtctg tcgtcggcac cccgaagagc gcggagcaga tccagcagga atgggacacg aacccgcgct ggaaggacgt cacccgcacc tactccgccg aggacgtcgt cgccctccag ggcagcgtgg tcgaggagca cacgctggcc cgccgcggtg cggaggtgct gtgggagcag ctgcacgacc tcgagtgggt caacgcgctg ggcgcgctga ccggcaacat ggccgtccag caggtgcgcg ccggcctgaa ggccatctac ctgtcgggct ggcaggtcgc cggcgatgcc aacctgtccg ggcacaccta ccccgaccag agcctgtatc ccgccaactc ggtgccgcag gtggtccgcc ggatcaacaa cgcactgcag cgcgccgacc agatcgccaa gatcgagggc gatacttcgg tggagaactg gctggcgccg attgtcgccg acggcgaggc cggctttggc ggcgcgctca acgtctacga gctgcagaaa gccctgatcg ccgcgggcgt tgcgggttcg cactgggagg accagttggc ctctgagaag aagtgcggcc acctgggcgg caaggtgttg atcccgaccc agcagcacat ccgcactttg acgtctgctc ggctcgcggc cgatgtggct gatgttccca cggtggtgat cgcccgtacc gacgccgagg cggccacgct gatcacctcc gacgtcgacg agcgcgacca gccgttcatc accggcgagc gcacccggga aggcttctac cgcaccaaga acggcatcga gccttgcatc gctcgggcga aggcctacgc cccgttcgcc gacttgatct ggatggagac cggtaccccg gacctcgagg ccgcccggca gttctccgag gcggtcaagg cggagtaccc ggaccagatg ctggcctaca actgctcgcc atcgttcaac tggaaaaagc acctcgacga cgccaccatc gccaagttcc agaaggagct ggcagccatg ggcttcaagt tccagttcat cacgctggcc ggcttccatg cgctgaacta ctcgatgttc gatctggcct acggctacgc ccagaaccag atgagcgcgt atgtcgaact gcaggaacgc gagttcgccg ccgaagaacg gggctacacc gcgaccaagc accagcgcga ggtcggcgcc ggctacttcg accggattgc caccaccgtg gacccgaatt cgtcgaccac cgcgttgacc ggttccaccg aagagggcca gttccactga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for MytuD.00014.a.B1.PW38797
PURIFICATION DATe: 2/3/2020
CONCENTRATION: 109mg/ml
OBSERVED MW: 48kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 3
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS VVGTPKSAEQ IQQEWDTNPR WKDVTRTYSA EDVVALQGSV VEEHTLARRG AEVLWEQLHD LEWVNALGAL TGNMAVQQVR AGLKAIYLSG WQVAGDANLS GHTYPDQSLY PANSVPQVVR RINNALQRAD QIAKIEGDTS VENWLAPIVA DGEAGFGGAL NVYELQKALI AAGVAGSHWE DQLASEKKCG HLGGKVLIPT QQHIRTLTSA RLAADVADVP TVVIARTDAE AATLITSDVD ERDQPFITGE RTREGFYRTK NGIEPCIARA KAYAPFADLI WMETGTPDLE AARQFSEAVK AEYPDQMLAY NCSPSFNWKK HLDDATIAKF QKELAAMGFK FQFITLAGFH ALNYSMFDLA YGYAQNQMSA YVELQEREFA AEERGYTATK HQREVGAGYF DRIATTVDPN SSTTALTGS
Validated NT Sequence
ggtggaaccg gtcaacgcgg tggtcgacga attcgggtcc acggtggtgg caatccggtc gaagtagccg gcgccgacct cgcgctggtg cttggtcgcg gtgtagcccc gttcttcggc ggcgaactcg cgttcctgca gttcgacata cgcgctcatc tggttctggg cgtagccgta ggccagatcg aacatcgagt agttcagcgc atggaagccg gccagcgtga tgaactggaa cttgaagccc atggctgcca gctccttctg gaacttggcg atggtggcgt cgtcgaggtg ctttttccag ttgaacgatg gcgagcagtt gtaggccagc atctggtccg ggtactccgc cttgaccgcc tcggagaact gccgggcggc ctcgaggtcc ggggtaccgg tctccatcca gatcaagtcg gcgaacgggg cgtaggcctt cgcccgagcg atgcaaggct cgatgccgtt cttggtgcgg tagaagcctt cccgggtgcg ctcgccggtg atgaacggct ggtcgcgctc gtcgacgtcg gaggtgatca gcgtggccgc ctcggcgtcg gtacgggcga tcaccaccgt gggaacatca gccacatcgg ccgcgagccg agcagacgtc aaagtgcgga tgtgctgctg ggtcgggatc aacaccttgc cgcccaggtg gccgcacttc ttctcagagg ccaactggtc ctcccagtgc gaacccgcaa cgcccgcggc gatcagggct ttctgcagct cgtagacgtt gagcgcgccg ccaaagccgg cctcgccgtc ggcgacaatc ggcgccagcc agttctccac cgaagtatcg ccctcgatct tggcgatctg gtcggcgcgc tgcagtgcgt tgttgatccg gcggaccacc tgcggcaccg agttggcggg atacaggctc tggtcggggt aggtgtgccc ggacaggttg gcatcgccgg cgacctgcca gcccgacagg tagatggcct tcaggccggc gcgcacctgc tggacggcca tgttgccggt cagcgcgccc agcgcgttga cccactcgag gtcgtgcagc tgctcccaca gcacctccgc accgcggcgg gccagcgtgt gctcctcgac cacgctgccc tggagggcga cgacgtcctc ggcggagtag gtgcgggtga cgtccttcca gcgcgggttc gtgtcccatt cctgctggat ctgctccgcg ctcttcgggg tgccgacgac agacatatgg tggtggtggt ggtgagccat
Expressed Protein Sequence
MAHHHHHHMS VVGTPKSAEQ IQQEWDTNPR WKDVTRTYSA EDVVALQGSV VEEHTLARRG AEVLWEQLHD LEWVNALGAL TGNMAVQQVR AGLKAIYLSG WQVAGDANLS GHTYPDQSLY PANSVPQVVR RINNALQRAD QIAKIEGDTS VENWLAPIVA DGEAGFGGAL NVYELQKALI AAGVAGSHWE DQLASEKKCG HLGGKVLIPT QQHIRTLTSA RLAADVADVP TVVIARTDAE AATLITSDVD ERDQPFITGE RTREGFYRTK NGIEPCIARA KAYAPFADLI WMETGTPDLE AARQFSEAVK AEYPDQMLAY NCSPSFNWKK HLDDATIAKF QKELAAMGFK FQFITLAGFH ALNYSMFDLA YGYAQNQMSA YVELQEREFA AEERGYTATK HQREVGAGYF DRIATTVDPN SSTTALTGST EEGQFH
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTCTG TCGTCGGCAC CCCGAAGAGC GCGGAGCAGA TCCAGCAGGA ATGGGACACG AACCCGCGCT GGAAGGACGT CACCCGCACC TACTCCGCCG AGGACGTCGT CGCCCTCCAG GGCAGCGTGG TCGAGGAGCA CACGCTGGCC CGCCGCGGTG CGGAGGTGCT GTGGGAGCAG CTGCACGACC TCGAGTGGGT CAACGCGCTG GGCGCGCTGA CCGGCAACAT GGCCGTCCAG CAGGTGCGCG CCGGCCTGAA GGCCATCTAC CTGTCGGGCT GGCAGGTCGC CGGCGATGCC AACCTGTCCG GGCACACCTA CCCCGACCAG AGCCTGTATC CCGCCAACTC GGTGCCGCAG GTGGTCCGCC GGATCAACAA CGCACTGCAG CGCGCCGACC AGATCGCCAA GATCGAGGGC GATACTTCGG TGGAGAACTG GCTGGCGCCG ATTGTCGCCG ACGGCGAGGC CGGCTTTGGC GGCGCGCTCA ACGTCTACGA GCTGCAGAAA GCCCTGATCG CCGCGGGCGT TGCGGGTTCG CACTGGGAGG ACCAGTTGGC CTCTGAGAAG AAGTGCGGCC ACCTGGGCGG CAAGGTGTTG ATCCCGACCC AGCAGCACAT CCGCACTTTG ACGTCTGCTC GGCTCGCGGC CGATGTGGCT GATGTTCCCA CGGTGGTGAT CGCCCGTACC GACGCCGAGG CGGCCACGCT GATCACCTCC GACGTCGACG AGCGCGACCA GCCGTTCATC ACCGGCGAGC GCACCCGGGA AGGCTTCTAC CGCACCAAGA ACGGCATCGA GCCTTGCATC GCTCGGGCGA AGGCCTACGC CCCGTTCGCC GACTTGATCT GGATGGAGAC CGGTACCCCG GACCTCGAGG CCGCCCGGCA GTTCTCCGAG GCGGTCAAGG CGGAGTACCC GGACCAGATG CTGGCCTACA ACTGCTCGCC ATCGTTCAAC TGGAAAAAGC ACCTCGACGA CGCCACCATC GCCAAGTTCC AGAAGGAGCT GGCAGCCATG GGCTTCAAGT TCCAGTTCAT CACGCTGGCC GGCTTCCATG CGCTGAACTA CTCGATGTTC GATCTGGCCT ACGGCTACGC CCAGAACCAG ATGAGCGCGT ATGTCGAACT GCAGGAACGC GAGTTCGCCG CCGAAGAACG GGGCTACACC GCGACCAAGC ACCAGCGCGA GGTCGGCGCC GGCTACTTCG ACCGGATTGC CACCACCGTG GACCCGAATT CGTCGACCAC CGCGTTGACC GGTTCCACCG AAGAGGGCCA GTTCCACTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT